>VFH121 (VFH121Q) /CSM/VF/VFH1-A/VFH121Q.Seq.d/
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCA
GATAACACCTACAAAGTACTCGTCGATAACAAXXXXXXXXXXAGAAAGCTGGCGAAAAAT
TAGCNGCCGAAAAAGNTGCTGAAAAANAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAG
TCGCTGAAGAAGATTTAGTTAAAACTGATGGCAAAAAAGAAGAAGGCAAAAAATCAACCA
AAAAAG

nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKK
EINKVETDQLTHQYTLVISPDNTYKVLVDN---

---KAGEKLAAEKXAEKXAEEADEEEEEVAEEDLVKTDGKKEEGKKSTKK

Frame A:
nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKK
EINKVETDQLTHQYTLVISPDNTYKVLVDN---

---rklakn*xpkkxlkxkpkklmkkkkkslkki*lklmakkkkaknqpkk

Frame B:
ispiiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllv
igikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*r
mnkklivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srk
kstklkpin*lintlwsfhqitptkyssit---

---eswrkisxrkxc*kxsrrs**rrrrsr*rrfs*n*wqkrrrqkinqk

Frame C:
flqssyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**
la*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke
*tks*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqer
nqqs*nrsinssihfghftr*hlqstrr*q---

---KAGEKLAAEKXAEKXAEEADEEEEEVAEEDLVKTDGKKEEGKKSTKK

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFH121 (VFH121Q) /CSM/VF/VFH1-A/VFH121Q.Seq.d/                       1063   0.0
VFM435 (VFM435Q) /CSM/VF/VFM4-B/VFM435Q.Seq.d/                       1027   0.0
VFM276 (VFM276Q) /CSM/VF/VFM2-D/VFM276Q.Seq.d/                       1027   0.0
VFH144 (VFH144Q) /CSM/VF/VFH1-B/VFH144Q.Seq.d/                       1027   0.0
VFE671 (VFE671Q) /CSM/VF/VFE6-C/VFE671Q.Seq.d/                       1027   0.0
VFD485 (VFD485Q) /CSM/VF/VFD4-D/VFD485Q.Seq.d/                       1027   0.0
VFD121 (VFD121Q) /CSM/VF/VFD1-A/VFD121Q.Seq.d/                       1027   0.0
VFA823 (VFA823Q) /CSM/VF/VFA8-A/VFA823Q.Seq.d/                       1027   0.0
VFA820 (VFA820Q) /CSM/VF/VFA8-A/VFA820Q.Seq.d/                       1027   0.0
SLE570 (SLE570Q) /CSM/SL/SLE5-C/SLE570Q.Seq.d/                       1027   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1019   0.0    3
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   6e-24  3
BZ392863|BZ392863.1 EINBW38TF EI_10_12_KB Entamoeba invadens genomic clone EINBW38, DNA sequence.    52   9e-07  2
BI876472|BI876472.1 fl72h01.y1 Sugano Kawakami zebrafish DRA Danio rerio cDNA clone 3819504 5' similar to TR:Q9PUC1 Q9PUC1 CALRETICULIN. ;, mRNA sequence.    42   5e-04  2
CB086220|CB086220.1 hj81f04.g1 Hedyotis centranthoides flower - Stage 2 (NYBG) Hedyotis centranthoides cDNA clone hj81f04, mRNA sequence.    36   7e-04  3
CB937139|CB937139.1 IpCGJx13_12_E07_23 IpCGJx13 Ictalurus punctatus cDNA clone IpCGJx13_12_E07 5', mRNA sequence.    56   8e-04  1
BI468042|BI468042.1 EST00452 Atlantic salmon Lambda Zap Express liver cDNA library Salmo salar cDNA clone LRR7-f02 5' similar to Calreticulin, mRNA sequence.    56   8e-04  1
CK420742|CK420742.1 AUF_IpTrk_27_j08 Trunk kidney cDNA library Ictalurus punctatus cDNA 5' similar to ER-resident chaperone calreticulin, mRNA sequence.    56   8e-04  1
AY342298|AY342298.1 Ictalurus punctatus ER-resident chaperone calreticulin mRNA, complete cds.    56   8e-04  1
AL374953|AL374953.1 Medicago truncatula EST MtBB10D04F2 : T3 end of clone MtBB10D04 of  cDNA library MtBB from symbiotic root nodules of cultivar Jemalong  of Medicago truncatula (barrel medic).    40   0.002  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   357   3e-97
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   226   6e-58
AK318734_1(AK318734|pid:none) Arabidopsis thaliana AT1G56340 mRN...   226   8e-58
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   225   1e-57
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   225   1e-57
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   223   4e-57
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   223   5e-57
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   223   5e-57
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   223   5e-57
AY086745_1(AY086745|pid:none) Arabidopsis thaliana clone 27210 m...   222   9e-57

 psg: 1.01  gvh: 0.69  alm: 0.49  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum

>> prediction for VFH121 is exc

>VFH121F.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCA
GATAACACCTACAAAGTACTCGTCGATAACAA----------

>VFH121Z.Seq
----------AGAAAGCTGGCGAAAAATTAGCNGCCGAAAAAGNTGCTGAAAAANAAGCC
GAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGGC
AAAAAAGAAGAAGGCAAAAAATCAACCAAAAAAG

>VFH121P.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCA
GATAACACCTACAAAGTACTCGTCGATAACAA----------AGAAAGCTGGCGAAAAAT
TAGCNGCCGAAAAAGNTGCTGAAAAANAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAG
TCGCTGAAGAAGATTTAGTTAAAACTGATGGCAAAAAAGAAGAAGGCAAAAAATCAACCA
AAAAAG