>VFG862 (VFG862Q) /CSM/VF/VFG8-C/VFG862Q.Seq.d/
GTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACT
ATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCT
AGAAGAAGAGTTCCACAAAAATGGTTAAACAGATTATCTGCTCTCAATGCTGGTACCACA
ATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCA
GGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAG
AAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCA
AGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCA
GGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTC
GCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTTA
GGTCXXXXXXXXXXAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTTAGG
TCTTGCTTTCCAATCCATCTCTGTTAATTCAATTCCACCAGTCTTTTACAACATGTTATC
ACAAGGTTTAGTTTCATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAGGTGCCAA
CGGTGGTGAACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGTGACATTACCTA
CGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGATGG
TCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCACT
CATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTT
AAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACCAT
CACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGA
GTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTT
CTGGATCCTTGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGGTAATAA
ACAAGTTGGTTTCGCAACTGCCATTCAAGGTTAAATTTTTTTAATTAATTTATATTTAAG
ATAG

vt*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWLNRLSALNAGTT
IPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGAS
STYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIAFDFAKFDGIL
G---

---PGIAFDFAKFDGILGLAFQSISVNSIPPVFYNMLSQGLVSSTLFSFWLSRTPGANGG
ELSFGSIDNTKYTGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSLIA
GPMADITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFVLTPKEYVLEVTEFG
KTECLSGFMGIELNMGNFWILGDVFISAYYTVFDFGNKQVGFATAIQG*iflinlylr*

Frame A:
vt*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWLNRLSALNAGTT
IPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGAS
STYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIAFDFAKFDGIL
G---

---nqvllsispnsmvf*vllsnpslliqfhqsfttcyhkv*fhqhsspsgyqelqvptv
vnshsvqsitpntlvtlptsh*ptkpignslwmtllsmvnqlvsvvllvtqfaiqvhhsl
lvqwlillpsmkn*vlss*mvkvsslivalstpyqmlpspllvvnlf*lqkntf*kllss
erlnv*vdlwvss*tweisgslvmfsslltilysilvinklvsqlpfkvkff*liyi*d

Frame B:
spdlhkrk*nylf*lyf*lllf*lkl*qyh*tsiklqenleeefhkng*tdyllsmlvpq
sqfqilkmlntmvplplvpqvkpsk*fsilvhptcgfhqrnvqslllhviyitnitavpq
ahmsptelispsntvvvlcqvlslkipsllvh*llkinyslkplpnqvllsispnsmvf*
v---

---trycfrfrqirwyfrscfpihlc*fnstsllqhvitrfsfintllllviknsrcqrw
*tlirfnr*hqihw*hylrpinqrnllgiryg*lcyrwsiswflwyylsrnlrfryithc
wsng*yycpq*kircchlkw*rcll*l*ryqhltkcyhhrcws*icfnskrirfrsy*vr
kd*mfewiygyrvkhgkfldpw*cfhlcllycirfw**tswfrnchsrlnffn*fifki

Frame C:
hltyikeneityfnfifsyycfsssfnstiklpssfkri*kksstkmvkqiicsqcwyhn
pnfrf*rcsilwchyhwyprsslqssfrywfiqlvdsikemsnhcccm*ft*qi*qrclk
hicrqrn*fhhpir*wcyvrfclsrfrhcwfinc*rsiir*shcrtrycfrfrqirwyfr
---

---PGIAFDFAKFDGILGLAFQSISVNSIPPVFYNMLSQGLVSSTLFSFWLSRTPGANGG
ELSFGSIDNTKYTGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSLIA
GPMADITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFVLTPKEYVLEVTEFG
KTECLSGFMGIELNMGNFWILGDVFISAYYTVFDFGNKQVGFATAIQG*iflinlylr*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFG862 (VFG862Q) /CSM/VF/VFG8-C/VFG862Q.Seq.d/                       2405   0.0
VFM519 (VFM519Q) /CSM/VF/VFM5-A/VFM519Q.Seq.d/                       1366   0.0
VFM401 (VFM401Q) /CSM/VF/VFM4-A/VFM401Q.Seq.d/                       1366   0.0
VFM234 (VFM234Q) /CSM/VF/VFM2-B/VFM234Q.Seq.d/                       1366   0.0
VFL828 (VFL828Q) /CSM/VF/VFL8-B/VFL828Q.Seq.d/                       1366   0.0
VFL410 (VFL410Q) /CSM/VF/VFL4-A/VFL410Q.Seq.d/                       1366   0.0
VFL274 (VFL274Q) /CSM/VF/VFL2-D/VFL274Q.Seq.d/                       1366   0.0
VFL252 (VFL252Q) /CSM/VF/VFL2-C/VFL252Q.Seq.d/                       1366   0.0
VFI770 (VFI770Q) /CSM/VF/VFI7-C/VFI770Q.Seq.d/                       1366   0.0
VFI689 (VFI689Q) /CSM/VF/VFI6-D/VFI689Q.Seq.d/                       1366   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Y16962|Y16962.1 Dictyostelium discoideum mRNA for cathepsin D.   1350   0.0    2
AJ243946|AJ243946.1 Dictyostelium discoideum ctsD gene for cathepsin D, exons 1 to 2.  1350   0.0    2
E33916|E33916.1 Candida boidinii strain with lowered protease activity and  utilization thereof as host for producing foreign protein.    36   3e-06  5
AX059531|AX059531.1 Sequence 264 from Patent WO0055325.            62   2e-05  1
AF076243|AF076243.1 Arabidopsis thaliana BAC T26N6 from chromosome IV at 19.3 cM, complete sequence.    62   2e-05  1
BX838383|BX838383.1 Arabidopsis thaliana Full-length cDNA 5PRIM end of clone  GSLTSIL89ZD03 of Silique of strain col-0 of Arabidopsis thaliana  (thale cress).    62   2e-05  1
CB264640|CB264640.1 48-E014661-035-002-P12-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000P122Q 5-PRIME, mRNA sequence.    62   2e-05  1
BE525959|BE525959.1 M64C07STM Arabidopsis developing seed Arabidopsis thaliana cDNA clone 600034526R1 5', mRNA sequence.    62   2e-05  1
BX827174|BX827174.1 Arabidopsis thaliana Full-length cDNA Complete sequence from clone  GSLTLS1ZB10 of Adult vegetative tissue of strain col-0 of  Arabidopsis thaliana (thale cress).    62   2e-05  1
CD825453|CD825453.1 BN25.060N03F011129 BN25 Brassica napus cDNA clone BN25060N03, mRNA sequence.    62   2e-05  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU259064_1(EU259064|pid:none) Epinephelus coioides pepsinogen A2...   162   9e-68
AF156787_1(AF156787|pid:none) Pleuronectes americanus pepsinogen...   156   3e-67
EU807926_1(EU807926|pid:none) Siniperca scherzeri pepsinogen A1 ...   155   3e-67
AB440200_1(AB440200|pid:none) Thunnus orientalis PGA mRNA for pe...   160   4e-67
EU807925_1(EU807925|pid:none) Siniperca chuatsi pepsinogen A1 pr...   154   1e-66
AF156788_1(AF156788|pid:none) Pleuronectes americanus pepsinogen...   169   2e-65
EU163285_1(EU163285|pid:none) Diplodus sargus pepsinogen mRNA, c...   167   5e-65
AB120129_1(AB120129|pid:none) Paralichthys olivaceus pep mRNA fo...   165   3e-64
AJ237674_1(AJ237674|pid:none) Cynara cardunculus mRNA for prepro...   135   2e-63
AJ550951_1(AJ550951|pid:none) Trematomus bernacchii mRNA for pep...   149   2e-63

 psg: 0.98  gvh: 0.77  alm: 0.41  top: 0.57  tms: 0.00  mit: 0.36  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: extracellular, including cell wall
	 20.0 %: mitochondrial
	 16.0 %: nuclear
	 12.0 %: vacuolar
	  4.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for VFG862 is exc

>VFG862F.Seq
GTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACT
ATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCT
AGAAGAAGAGTTCCACAAAAATGGTTAAACAGATTATCTGCTCTCAATGCTGGTACCACA
ATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCA
GGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAG
AAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCA
AGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCA
GGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTC
GCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTTA
GGTC----------

>VFG862Z.Seq
----------AACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTTAGGTCTT
GCTTTCCAATCCATCTCTGTTAATTCAATTCCACCAGTCTTTTACAACATGTTATCACAA
GGTTTAGTTTCATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAGGTGCCAACGGT
GGTGAACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGTGACATTACCTACGTC
CCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAA
TCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATT
GCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAAT
GGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACC
GTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTC
GGAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGG
ATCCTTGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAA
GTTGGTTTCGCAACTGCCATTCAAGGTTAAATTTTTTTAATTAATTTATATTTAAGATAG

>VFG862P.Seq
GTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACT
ATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCT
AGAAGAAGAGTTCCACAAAAATGGTTAAACAGATTATCTGCTCTCAATGCTGGTACCACA
ATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCA
GGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAG
AAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCA
AGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCA
GGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTC
GCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTTA
GGTC----------AACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTTAGG
TCTTGCTTTCCAATCCATCTCTGTTAATTCAATTCCACCAGTCTTTTACAACATGTTATC
ACAAGGTTTAGTTTCATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAGGTGCCAA
CGGTGGTGAACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGTGACATTACCTA
CGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGATGG
TCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCACT
CATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTT
AAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACCAT
CACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGA
GTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTT
CTGGATCCTTGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGGTAATAA
ACAAGTTGGTTTCGCAACTGCCATTCAAGGTTAAATTTTTTTAATTAATTTATATTTAAG
ATAG