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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM558
Library AF
(Link to library)
Clone ID AFM558
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM558P
(Link to Original site)
Representative DNA sequence 
>AFM558 (AFM558Q) /CSM/AF/AFM5-C/AFM558Q.Seq.d/
GCATATACATATACATTAAATAATGGCCACATTATCACGTAATTTACGTTTATCATTAGA
TACACCAACCTCAACTTTTGTTAGAACCAAAATTGTTTGTACAATTGGTCCAAAAACAAT
GAGTGAAGAAGCTTTAATTAAATTAATCGAAACTGGTATGAACGTTTGTAGACTCAACTT
TTCACATGGTACTCATGATTATCATGGTCAAGTTATTAAAAATGTTAGATCAGCAATGGA
GAAAACAGGTAAAATTATTGCTATTATGTTAGATACTAAAGGTCCAGAAATTAGAACTGG
TAAAATTGAAGATCGTTGTGGTTATGTTGATTTATTCGTTGGCCAAGAAATCCTCGTTGA
TACCAATATGAATCAACCAGGTACTTCATTTAGAATTTCAATCGATTATAAAGGTTTATT
AGATTCAGTTAAAGTTGGTGGTTACATTTTAATTGCTGATGGTGTAATTTCATTATCTAT
TACTGCAGTTGAAAAAGAAAAAGGTCATGTTGTTTGTAGAGTTAATAATAATTCACGTCT
TGGTGAAAATAAAAAATGTACATTTACCXXXXXXXXXXTCGTCGCTCAAAAGATGATCGT
TAGTAAATGTAATGCCGCCGGTAAACCAGTAATTACCGCCACTCAAAATGTTGGAATCAA
TGATCAAGAATCCAAGACCAACCCGTGCTGAAGCTACCGATGTTGCCAATGCCGTTTTAG
ATGGTTCCGATTGTGTTATGCTTTCAGGTGAAACCGCAAGCGGTGATTACCCATACGAAG
CCGTAGACATTATGGCAAAGATCTGTAGAGAAGCAGAGTTGGTAGAATCTTCAACTGATT
ATCAAACTTTATTCGCTGCCCTTAAATTATCATCAGCCAAACCAGTCAGCATTGCTGAAA
CCGTTGCTTCTTACGCCGTCGCAACCGCTATCGATTTGAAGGCTGATCTCATCATCACAC
TCACTGAAACCGGTCTCACTGCTCGTCTCGTCAGTAAATATCGTCCATCCATTCCAATCA
TTGCCGTCACTTCATGGTCATACACTGTTAAACATTTACTCGCCACTAGAGGTGCCATTC
CATTCTTGGTAGAATCATTGGTCGGCACTGATAAACTCGTTGAATCTTGTCTCGAATATG
CTATGAAACATAACTTATGTAAAAAAGGTTCAAGAGTTGTCATTGTTTCTGGTGTTATGG
AAGGTGTACCAGGTAAAACCAACAGTTTGAGAGTTCTTACCGTTGGTGAATCAATCAAAG
ATTTAAAGAAAAAATAAAAATATAGCCATAATTTTAATAAGCGCA
sequence update 2009. 4. 3
Translated Amino Acid sequence 
hihih*IMATLSRNLRLSLDTPTSTFVRTKIVCTIGPKTMSEEALIKLIETGMNVCRLNF
SHGTHDYHGQVIKNVRSAMEKTGKIIAIMLDTKGPEIRTGKIEDRCGYVDLFVGQEILVD
TNMNQPGTSFRISIDYKGLLDSVKVGGYILIADGVISLSITAVEKEKGHVVCRVNNNSRL
GENKKCTFT---

---VAQKMIVSKCNAAGKPVITATQNVGINDQESKTNPC*syrccqcrfrwfrlcyafr*
nrkr*lpirsrrhygkdl*rsrvgrifn*lsnfircp*iiisqtsqhc*nrcflrrrnry
rfeg*shhhth*nrshcssrq*issihsnhcrhfmvihc*tftrh*rchsilgriigrh*
*tr*ilsricyet*lm*krfkschcfwcygrctr*nqqfessyrw*inqrfkekiki*p*
f**a
Translated Amino Acid sequence (All Frames) 
Frame A:
aytytlnnghiit*ftfiirytnlnfc*nqnclynwsknne*rsfn*inrnwyerl*tql
ftwys*lswssy*kc*isngenr*nycyyvry*rsrn*nw*n*rslwlc*firwprnpr*
yqyestryfi*nfnrl*rfirfs*swwlhfnc*wcnfiiyycs*krkrsccl*s***fts
w*k*kmyiy---

---sslkr*slvnvmppvnq*lpplkmlesmiknprptraeatdvanavldgsdcvmlsg
etasgdypyeavdimakicreaelvesstdyqtlfaalklssakpvsiaetvasyavata
idlkadliitltetgltarlvskyrpsipiiavtswsytvkhllatrgaipflveslvgt
dklvescleyamkhnlckkgsrvvivsgvmegvpgktnslrvltvgesikdlkkk*kysh
nfnkr

Frame B:
hihih*IMATLSRNLRLSLDTPTSTFVRTKIVCTIGPKTMSEEALIKLIETGMNVCRLNF
SHGTHDYHGQVIKNVRSAMEKTGKIIAIMLDTKGPEIRTGKIEDRCGYVDLFVGQEILVD
TNMNQPGTSFRISIDYKGLLDSVKVGGYILIADGVISLSITAVEKEKGHVVCRVNNNSRL
GENKKCTFT---

---rrskddr**m*crr*tsnyrhskcwnq*sriqdqpvlklpmlpmpf*mvpivlcfqv
kpqavithtkp*tlwqrsvekqsw*nlqliiklyslplnyhqpnqsallkpllltpsqpl
si*rlissshslkpvsllvssvnivhpfqslpslhghtllniysplevpfhsw*nhwsal
inslnlvsnml*nityvkkvqelslflvlwkvyqvkptv*eflplvnqski*rknkniai
ilisa

Frame C:
iyiyik*wphyhviyvyh*ihqpqlllepklfvqlvqkq*vkkl*ln*sklv*tfvdstf
hmvlmiimvkllkmldqqwrkqvkllllc*ilkvqklelvklkivvvmliyslakkssli
pi*inqvlhlefqsiikvy*iqlklvvtf*llmv*fhylllqlkkkkvmlfveliiihvl
vkiknvhl---

---VAQKMIVSKCNAAGKPVITATQNVGINDQESKTNPC*syrccqcrfrwfrlcyafr*
nrkr*lpirsrrhygkdl*rsrvgrifn*lsnfircp*iiisqtsqhc*nrcflrrrnry
rfeg*shhhth*nrshcssrq*issihsnhcrhfmvihc*tftrh*rchsilgriigrh*
*tr*ilsricyet*lm*krfkschcfwcygrctr*nqqfessyrw*inqrfkekiki*p*
f**a
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM558 (AFM558Q) /CSM/AF/AFM5-C/AFM558Q.Seq.d/                       2456   0.0
SFJ336 (SFJ336Q) /CSM/SF/SFJ3-B/SFJ336Q.Seq.d/                       1362   0.0
SFI280 (SFI280Q) /CSM/SF/SFI2-D/SFI280Q.Seq.d/                       1360   0.0
AFK736 (AFK736Q) /CSM/AF/AFK7-B/AFK736Q.Seq.d/                       1358   0.0
SFI138 (SFI138Q) /CSM/SF/SFI1-B/SFI138Q.Seq.d/                       1346   0.0
SFC160 (SFC160Q) /CSM/SF/SFC1-C/SFC160Q.Seq.d/                       1346   0.0
SFB624 (SFB624Q) /CSM/SF/SFB6-A/SFB624Q.Seq.d/                       1346   0.0
CFJ485 (CFJ485Q) /CSM/CF/CFJ4-D/CFJ485Q.Seq.d/                       1346   0.0
CFC605 (CFC605Q) /CSM/CF/CFC6-A/CFC605Q.Seq.d/                       1346   0.0
CFC470 (CFC470Q) /CSM/CF/CFC4-C/CFC470Q.Seq.d/                       1346   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ347238) Dictyostelium discoideum cDNA clone:dda26c15, 3' ...  1368   0.0    2
(BJ402761) Dictyostelium discoideum cDNA clone:dds17o20, 3' ...  1360   0.0    2
(BJ401769) Dictyostelium discoideum cDNA clone:dds19h09, 3' ...  1360   0.0    2
(BJ343201) Dictyostelium discoideum cDNA clone:dda22h09, 3' ...  1356   0.0    2
(BJ343359) Dictyostelium discoideum cDNA clone:dda22g22, 3' ...  1346   0.0    2
(BJ399134) Dictyostelium discoideum cDNA clone:dds4g08, 3' e...  1249   0.0    2
(BJ401721) Dictyostelium discoideum cDNA clone:dds19n06, 3' ...  1245   0.0    2
(BJ402656) Dictyostelium discoideum cDNA clone:dds17i18, 3' ...  1227   0.0    3
(BJ402576) Dictyostelium discoideum cDNA clone:dds17k09, 3' ...  1227   0.0    3
(BJ401928) Dictyostelium discoideum cDNA clone:dds19i24, 3' ...  1227   0.0    3
dna update 2009. 4.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RF5) RecName: Full=Pyruvate kinase;          Short=PK;      ...   398   e-116
BC061541_1(BC061541|pid:none) Rattus norvegicus pyruvate kinase,...   135   3e-61
AB050726_1(AB050726|pid:none) Toxoplasma gondii pk mRNA for pyru...   191   2e-52
CR382401_10(CR382401|pid:none) Plasmodium falciparum chromosome ...   191   4e-52
AY608682_1(AY608682|pid:none) Achlya bisexualis pyruvate kinase ...   176   6e-47
AE015927_2237(AE015927|pid:none) Clostridium tetani E88, complet...   169   5e-46
AB005234_16(AB005234|pid:none) Arabidopsis thaliana genomic DNA,...   168   2e-45
FB912634_1(FB912634|pid:none) Sequence 131907 from Patent WO2008...   167   3e-45
FB912628_1(FB912628|pid:none) Sequence 131901 from Patent WO2008...   167   3e-45
FB912638_1(FB912638|pid:none) Sequence 131911 from Patent WO2008...   167   3e-45
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM558 is

5' end seq. ID AFM558F
5' end seq. 
>AFM558F.Seq
GCATATACATATACATTAAATAATGGCCACATTATCACGTAATTTACGTTTATCATTAGA
TACACCAACCTCAACTTTTGTTAGAACCAAAATTGTTTGTACAATTGGTCCAAAAACAAT
GAGTGAAGAAGCTTTAATTAAATTAATCGAAACTGGTATGAACGTTTGTAGACTCAACTT
TTCACATGGTACTCATGATTATCATGGTCAAGTTATTAAAAATGTTAGATCAGCAATGGA
GAAAACAGGTAAAATTATTGCTATTATGTTAGATACTAAAGGTCCAGAAATTAGAACTGG
TAAAATTGAAGATCGTTGTGGTTATGTTGATTTATTCGTTGGCCAAGAAATCCTCGTTGA
TACCAATATGAATCAACCAGGTACTTCATTTAGAATTTCAATCGATTATAAAGGTTTATT
AGATTCAGTTAAAGTTGGTGGTTACATTTTAATTGCTGATGGTGTAATTTCATTATCTAT
TACTGCAGTTGAAAAAGAAAAAGGTCATGTTGTTTGTAGAGTTAATAATAATTCACGTCT
TGGTGAAAATAAAAAATGTACATTTACCXXXXXXXXXX
Length of 5' end seq. 578
3' end seq. ID AFM558Z
3' end seq. 
>AFM558Z.Seq
XXXXXXXXXXTCGTCGCTCAAAAGATGATCGTTAGTAAATGTAATGCCGCCGGTAAACCA
GTAATTACCGCCACTCAAAATGTTGGAATCAATGATCAAGAATCCAAGACCAACCCGTGC
TGAAGCTACCGATGTTGCCAATGCCGTTTTAGATGGTTCCGATTGTGTTATGCTTTCAGG
TGAAACCGCAAGCGGTGATTACCCATACGAAGCCGTAGACATTATGGCAAAGATCTGTAG
AGAAGCAGAGTTGGTAGAATCTTCAACTGATTATCAAACTTTATTCGCTGCCCTTAAATT
ATCATCAGCCAAACCAGTCAGCATTGCTGAAACCGTTGCTTCTTACGCCGTCGCAACCGC
TATCGATTTGAAGGCTGATCTCATCATCACACTCACTGAAACCGGTCTCACTGCTCGTCT
CGTCAGTAAATATCGTCCATCCATTCCAATCATTGCCGTCACTTCATGGTCATACACTGT
TAAACATTTACTCGCCACTAGAGGTGCCATTCCATTCTTGGTAGAATCATTGGTCGGCAC
TGATAAACTCGTTGAATCTTGTCTCGAATATGCTATGAAACATAACTTATGTAAAAAAGG
TTCAAGAGTTGTCATTGTTTCTGGTGTTATGGAAGGTGTACCAGGTAAAACCAACAGTTT
GAGAGTTCTTACCGTTGGTGAATCAATCAAAGATTTAAAGAAAAAATAAAAATATAGCCA
TAATTTTAATAAGCGCA
Length of 3' end seq. 737
Connected seq. ID AFM558P
Connected seq. 
>AFM558P.Seq
GCATATACATATACATTAAATAATGGCCACATTATCACGTAATTTACGTTTATCATTAGA
TACACCAACCTCAACTTTTGTTAGAACCAAAATTGTTTGTACAATTGGTCCAAAAACAAT
GAGTGAAGAAGCTTTAATTAAATTAATCGAAACTGGTATGAACGTTTGTAGACTCAACTT
TTCACATGGTACTCATGATTATCATGGTCAAGTTATTAAAAATGTTAGATCAGCAATGGA
GAAAACAGGTAAAATTATTGCTATTATGTTAGATACTAAAGGTCCAGAAATTAGAACTGG
TAAAATTGAAGATCGTTGTGGTTATGTTGATTTATTCGTTGGCCAAGAAATCCTCGTTGA
TACCAATATGAATCAACCAGGTACTTCATTTAGAATTTCAATCGATTATAAAGGTTTATT
AGATTCAGTTAAAGTTGGTGGTTACATTTTAATTGCTGATGGTGTAATTTCATTATCTAT
TACTGCAGTTGAAAAAGAAAAAGGTCATGTTGTTTGTAGAGTTAATAATAATTCACGTCT
TGGTGAAAATAAAAAATGTACATTTACC----------TCGTCGCTCAAAAGATGATCGT
TAGTAAATGTAATGCCGCCGGTAAACCAGTAATTACCGCCACTCAAAATGTTGGAATCAA
TGATCAAGAATCCAAGACCAACCCGTGCTGAAGCTACCGATGTTGCCAATGCCGTTTTAG
ATGGTTCCGATTGTGTTATGCTTTCAGGTGAAACCGCAAGCGGTGATTACCCATACGAAG
CCGTAGACATTATGGCAAAGATCTGTAGAGAAGCAGAGTTGGTAGAATCTTCAACTGATT
ATCAAACTTTATTCGCTGCCCTTAAATTATCATCAGCCAAACCAGTCAGCATTGCTGAAA
CCGTTGCTTCTTACGCCGTCGCAACCGCTATCGATTTGAAGGCTGATCTCATCATCACAC
TCACTGAAACCGGTCTCACTGCTCGTCTCGTCAGTAAATATCGTCCATCCATTCCAATCA
TTGCCGTCACTTCATGGTCATACACTGTTAAACATTTACTCGCCACTAGAGGTGCCATTC
CATTCTTGGTAGAATCATTGGTCGGCACTGATAAACTCGTTGAATCTTGTCTCGAATATG
CTATGAAACATAACTTATGTAAAAAAGGTTCAAGAGTTGTCATTGTTTCTGGTGTTATGG
AAGGTGTACCAGGTAAAACCAACAGTTTGAGAGTTCTTACCGTTGGTGAATCAATCAAAG
ATTTAAAGAAAAAATAAAAATATAGCCATAATTTTAATAAGCGCA
Length of connected seq. 1295
Full length Seq ID -
Full length Seq. -
Length of full length seq. -