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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFG517
Library VF
(Link to library)
Clone ID VFG517
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12898-1
Original site URL
Representative seq. ID VFG517E
(Link to Original site)
Representative DNA sequence 
>VFG517 (VFG517Q) /CSM/VF/VFG5-A/VFG517Q.Seq.d/
AACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTAGAGTTTATGTTGATG
GTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGACAAGCAAGAGAATTAG
GAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAAAAAATAAAGGTCCAC
CAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTAAATGGGCAGATGAAG
TTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATAGTTTAAACTGTGATT
TTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAGATGTTTATGAAGGCA
TTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTGTTTCAACAACCGAGT
TGGTTGGTCGTATGTTATTATGCACCAAAGGCCATCTTCAAAATGTTTCAGGTGAGCAAA
CCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAATCACCATACACAAGTT
TATCACATTTCCTCCCAACCNCCAGAAANATNGTCCAATTNTCANAAGGTCGTTCNCCAA
AACCAAACGATAAAATCATTTATATGGATGGTGGTTTCGATTTATTCCATGTAGGTCATA
CTGAGGCTTTGAAACAAGCAANAGCACTCGGTGACTATCTCATCGTTGGTGTTCATGATG
ATANAGTAGTTCNCGAACAAAAAGGTAGCAACTTCCCAATTATGAATTTACATGAACGTG
NACTCTCTGNACTCTCATGTCGTTATGTCGATGAAGTTGTCATTGGTGCTCCATTCAGTG
TCACCAAANATATGATTGACTCNCTTCACATCAATGTTGTCGTTCATGGTGATGATCAAG
TTGNACTCGGTCCANAAGGTGGTGTAGATCCANACAAACTCCCAANANAATTAGGTATTT
ACAAANAAGTTAAACATACTGAAGGTCNCNCTGCAACTGAAATCGTTAAAANAATCATTG
ATAATAGACTTCAATATGAAGCTCGNAATCGTAAAAAANAAGCAAAAGAAATTAATTTCA
TTGAACNATCNAATTAAAAAAAATTAAAAAATAAAAAATAAAAATAAAACNAATAAAAAA
AAAAATAGAAAAAAAATT
sequence update 2001. 6. 9
Translated Amino Acid sequence 
QVFTMSTTTNKKPIRVYVDGCFDLMHFGHANALRQARELGDILVVGVHTDEEIAKNKGPP
VMNEQERYKAVRACKWADEVAEGAPYTLTEEYLDSLNCDFCVHGEDISVGADGKDVYEGI
KKSGKFRFIKRTEGVSTTELVGRMLLCTKGHLQNVSGEQTSPLGGVNPNVLHKQSPYTSL
SHFLPTXRXXVQXSXGRSPKPNDKIIYMDGGFDLFHVGHTEALKQAXALGDYLIVGVHDD
XVVXEQKGSNFPIMNLHERXLSXLSCRYVDEVVIGAPFSVTKXMIDSLHINVVVHGDDQV
XLGPXGGVDPXKLPXXLGIYKXVKHTEGXXATEIVKXIIDNRLQYEARNRKKXAKEINFI
EXSN*kklknkk*k*nx*kkk*kkn
Translated Amino Acid sequence (All Frames) 
Frame A:
nkflq*vlqliksqlefmlmvvli*cildmqmh*dkqen*eif**lvyiqmkklqkikvh
ql*mnkkdikqyvhvngqmklqkvhlil*lkniwiv*tvifvymvkifqlvqmvkmfmka
lkrvvnldssnvlkvfqqpswlvvcyyapkaifkmfqvskphhwevsiqtfytnnhhtqv
yhissqppexxsnxxkvvxqnqtiksfiwmvvsiysm*vilrl*nkqxhsvtisslvfmm
ix*fxnkkvatsql*iymnvxslxshvvmsmklslvlhsvspxi*ltxftsmlsfmvmik
lxsvxkvv*ixtnsqxn*vftxklnilkvxlqlkslkxsliidfnmklxivkxkqkklis
lnxxikkn*kiknknktnkkknrkki


Frame B:
tsfyneyyn**kan*slc*wlf*fnafwtckciktskrirryfsswctyr*rnckk*rst
sye*trki*sstcm*mgr*scrrctlyfn*rifg*fkl*flctw*ryfswcrw*rcl*rh
*kew*i*ihqty*rcfnnrvgwsyvimhqrpsskcfr*anltigrcqskrftqtitihkf
itfppnxqkxxpixxrsfxktkr*nhlygwwfrfipcrsy*gfetsxstr*lshrwcs**
xsssrtkr*qlpnyeft*txtlxtlmslcr*schwcsiqchqxyd*lxshqccrsw**ss
xtrsxrwcrsxqtpxxirylqxs*ty*rsxcn*nr*xnh***tsi*ssxs*kxskrn*fh
*tixlkkikk*kikikxikkkiekk


Frame C:
QVFTMSTTTNKKPIRVYVDGCFDLMHFGHANALRQARELGDILVVGVHTDEEIAKNKGPP
VMNEQERYKAVRACKWADEVAEGAPYTLTEEYLDSLNCDFCVHGEDISVGADGKDVYEGI
KKSGKFRFIKRTEGVSTTELVGRMLLCTKGHLQNVSGEQTSPLGGVNPNVLHKQSPYTSL
SHFLPTXRXXVQXSXGRSPKPNDKIIYMDGGFDLFHVGHTEALKQAXALGDYLIVGVHDD
XVVXEQKGSNFPIMNLHERXLSXLSCRYVDEVVIGAPFSVTKXMIDSLHINVVVHGDDQV
XLGPXGGVDPXKLPXXLGIYKXVKHTEGXXATEIVKXIIDNRLQYEARNRKKXAKEINFI
EXSN*kklknkk*k*nx*kkk*kkn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFG517 (VFG517Q) /CSM/VF/VFG5-A/VFG517Q.Seq.d/                       1976   0.0
VFN722 (VFN722Q) /CSM/VF/VFN7-A/VFN722Q.Seq.d/                       1959   0.0
VFG365 (VFG365Q) /CSM/VF/VFG3-C/VFG365Q.Seq.d/                       1955   0.0
VFI626 (VFI626Q) /CSM/VF/VFI6-B/VFI626Q.Seq.d/                       1941   0.0
VFM413 (VFM413Q) /CSM/VF/VFM4-A/VFM413Q.Seq.d/                       1931   0.0
VFE318 (VFE318Q) /CSM/VF/VFE3-A/VFE318Q.Seq.d/                       1931   0.0
CFG421 (CFG421Q) /CSM/CF/CFG4-A/CFG421Q.Seq.d/                       1923   0.0
VFJ833 (VFJ833Q) /CSM/VF/VFJ8-B/VFJ833Q.Seq.d/                       1897   0.0
VFA266 (VFA266Q) /CSM/VF/VFA2-C/VFA266Q.Seq.d/                       1873   0.0
VFF530 (VFF530Q) /CSM/VF/VFF5-B/VFF530Q.Seq.d/                       1742   0.0
own update 2001.12. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BG140383|BG140383.1 EST480825 wild tomato pollen Lycopersicon pennellii cDNA clonecLPP17G1 5' sequence, mRNA sequence.    92   6e-16  3
BG131085|BG131085.1 EST463977 tomato crown gall Lycopersicon esculentum cDNA clone cTOE2D11 5' sequence, mRNA sequence.    92   1e-15  3
AW217896|AW217896.1 EST296610 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD1C15, mRNA sequence.    92   7e-15  2
AI778006|AI778006.1 EST258885 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES3N17, mRNA sequence.    92   8e-15  2
AW217261|AW217261.1 EST295975 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC89G22, mRNA sequence.    92   8e-15  2
AW219042|AW219042.1 EST301524 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX2H3, mRNA sequence.    92   9e-15  2
BG138134|BG138134.1 EST478576 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP8K18 5' sequence, mRNA sequence.    92   9e-15  2
BG642411|BG642411.1 EST355887 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD11C15 5' end, mRNA sequence.    92   1e-14  2
BG133537|BG133537.1 EST466525 tomato crown gall Lycopersicon esculentum cDNA clone cTOE13N15 5' sequence, mRNA sequence.    92   2e-14  1
BI935302|BI935302.1 EST555191 tomato flower, anthesis Lycopersicon esculentum cDNA clone cTOD22O12 5' end, mRNA sequence.    92   2e-14  1
dna update 2004. 3. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55BZ4) RecName: Full=Ethanolamine-phosphate cytidylyltransfera...   676   0.0
AE014134_2379(AE014134|pid:none) Drosophila melanogaster chromos...   362   1e-98
AK166319_1(AK166319|pid:none) Mus musculus mammary gland RCB-052...   357   3e-97
AL663030_9(AL663030|pid:none) Mouse DNA sequence from clone RP23...   357   4e-97
(Q5EA75) RecName: Full=Ethanolamine-phosphate cytidylyltransfera...   357   6e-97
BT077764_1(BT077764|pid:none) Lepeophtheirus salmonis Pacific fo...   355   2e-96
AY189524_1(AY189524|pid:none) Mus musculus CTP:ethanolaminephosp...   351   3e-95
BC088018_1(BC088018|pid:none) Xenopus tropicalis phosphate cytid...   348   2e-94
AM502250_130(AM502250|pid:none) Leishmania infantum chromosome 32.    342   1e-92
AK297996_1(AK297996|pid:none) Homo sapiens cDNA FLJ55495 complet...   341   3e-92
protein update 2009. 7.10
PSORT 

 psg: 0.52  gvh: 0.53  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: nuclear
	 28.0 %: mitochondrial
	 28.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFG517 is nuc
5' end seq. ID VFG517F
5' end seq. 
>VFG517F.Seq
AACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTAGAGTTTATGTTGATG
GTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGACAAGCAAGAGAATTAG
GAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAAAAAATAAAGGTCCAC
CAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTAAATGGGCAGATGAAG
TTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATAGTTTAAACTGTGATT
TTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAGATGTTTATGAAGGCA
TTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTGTTTCAACAACCGAGT
TGGTTGGTCGTATGTTATTATGCACCAAAGGCCATCTTCAAAATGTTTCAGGTGAGCAAA
CCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAATCACCATACACAAGTT
TATCACATTTCC----------
Length of 5' end seq. 552
3' end seq. ID VFG517Z
3' end seq. 
>VFG517Z.Seq
----------GTTTCAGGTGAGCAAACCTCACCATTGGGAGGTGTCAATCCAAACGTTTT
ACACAAACAATCACCATACACAAGTTTATCACATTTCCTCCCAACCNCCAGAAANATNGT
CCAATTNTCANAAGGTCGTTCNCCAAAACCAAACGATAAAATCATTTATATGGATGGTGG
TTTCGATTTATTCCATGTAGGTCATACTGAGGCTTTGAAACAAGCAANAGCACTCGGTGA
CTATCTCATCGTTGGTGTTCATGATGATANAGTAGTTCNCGAACAAAAAGGTAGCAACTT
CCCAATTATGAATTTACATGAACGTGNACTCTCTGNACTCTCATGTCGTTATGTCGATGA
AGTTGTCATTGGTGCTCCATTCAGTGTCACCAAANATATGATTGACTCNCTTCACATCAA
TGTTGTCGTTCATGGTGATGATCAAGTTGNACTCGGTCCANAAGGTGGTGTAGATCCANA
CAAACTCCCAANANAATTAGGTATTTACAAANAAGTTAAACATACTGAAGGTCNCNCTGC
AACTGAAATCGTTAAAANAATCATTGATAATAGACTTCAATATGAAGCTCGNAATCGTAA
AAAANAAGCAAAAGAAATTAATTTCATTGAACNATCNAATTAAAAAAAATTAAAAAATAA
AAAATAAAAATAAAACNAATAAAAAAAAAAATAGAAAAAAAATT
Length of 3' end seq. 694
Connected seq. ID VFG517P
Connected seq. 
>VFG517P.Seq
AACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTAGAGTTTATGTTGATG
GTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGACAAGCAAGAGAATTAG
GAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAAAAAATAAAGGTCCAC
CAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTAAATGGGCAGATGAAG
TTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATAGTTTAAACTGTGATT
TTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAGATGTTTATGAAGGCA
TTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTGTTTCAACAACCGAGT
TGGTTGGTCGTATGTTATTATGCACCAAAGGCCATCTTCAAAATGTTTCAGGTGAGCAAA
CCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAATCACCATACACAAGTT
TATCACATTTCC----------GTTTCAGGTGAGCAAACCTCACCATTGGGAGGTGTCAA
TCCAAACGTTTTACACAAACAATCACCATACACAAGTTTATCACATTTCCTCCCAACCNC
CAGAAANATNGTCCAATTNTCANAAGGTCGTTCNCCAAAACCAAACGATAAAATCATTTA
TATGGATGGTGGTTTCGATTTATTCCATGTAGGTCATACTGAGGCTTTGAAACAAGCAAN
AGCACTCGGTGACTATCTCATCGTTGGTGTTCATGATGATANAGTAGTTCNCGAACAAAA
AGGTAGCAACTTCCCAATTATGAATTTACATGAACGTGNACTCTCTGNACTCTCATGTCG
TTATGTCGATGAAGTTGTCATTGGTGCTCCATTCAGTGTCACCAAANATATGATTGACTC
NCTTCACATCAATGTTGTCGTTCATGGTGATGATCAAGTTGNACTCGGTCCANAAGGTGG
TGTAGATCCANACAAACTCCCAANANAATTAGGTATTTACAAANAAGTTAAACATACTGA
AGGTCNCNCTGCAACTGAAATCGTTAAAANAATCATTGATAATAGACTTCAATATGAAGC
TCGNAATCGTAAAAAANAAGCAAAAGAAATTAATTTCATTGAACNATCNAATTAAAAAAA
ATTAAAAAATAAAAAATAAAAATAAAACNAATAAAAAAAAAAATAGAAAAAAAATT
Length of connected seq. 1246
Full length Seq ID VFG517E
Full length Seq. 
>VFG517E.Seq
AACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTAGAGTTTATGTTGATG
GTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGACAAGCAAGAGAATTAG
GAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAAAAAATAAAGGTCCAC
CAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTAAATGGGCAGATGAAG
TTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATAGTTTAAACTGTGATT
TTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAGATGTTTATGAAGGCA
TTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTGTTTCAACAACCGAGT
TGGTTGGTCGTATGTTATTATGCACCAAAGGCCATCTTCAAAATGTTTCAGGTGAGCAAA
CCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAATCACCATACACAAGTT
TATCACATTTCCTCCCAACCNCCAGAAANATNGTCCAATTNTCANAAGGTCGTTCNCCAA
AACCAAACGATAAAATCATTTATATGGATGGTGGTTTCGATTTATTCCATGTAGGTCATA
CTGAGGCTTTGAAACAAGCAANAGCACTCGGTGACTATCTCATCGTTGGTGTTCATGATG
ATANAGTAGTTCNCGAACAAAAAGGTAGCAACTTCCCAATTATGAATTTACATGAACGTG
NACTCTCTGNACTCTCATGTCGTTATGTCGATGAAGTTGTCATTGGTGCTCCATTCAGTG
TCACCAAANATATGATTGACTCNCTTCACATCAATGTTGTCGTTCATGGTGATGATCAAG
TTGNACTCGGTCCANAAGGTGGTGTAGATCCANACAAACTCCCAANANAATTAGGTATTT
ACAAANAAGTTAAACATACTGAAGGTCNCNCTGCAACTGAAATCGTTAAAANAATCATTG
ATAATAGACTTCAATATGAAGCTCGNAATCGTAAAAAANAAGCAAAAGAAATTAATTTCA
TTGAACNATCNAATTAAAAAAAATTAAAAAATAAAAAATAAAAATAAAACNAATAAAAAA
AAAAATAGAAAAAAAATT
Length of full length seq. 1158