>VFG501 (VFG501Q) /CSM/VF/VFG5-A/VFG501Q.Seq.d/
XXXXXXXXXXTGCACGTAATGTTGTAAGACAAGTATGTGCTGGTGTTGAATATCTCCATT
CACAAGGTATTGCTCATAGAGATTTGAAACCAGAGAATTTATTATGTAATGGTGAAGGTG
AAGATATGACAATCAAAATTGCAGATTTTGGTCTTAGTAAAATATTTGGAACTGGTGAAG
CATTGGAAACAAGTTGTGGTACACCAGATTATGTTGCACCAGAAGTTTTAACTGGTGGTT
CATATGATAATGCAGTCGATATGTGGTCAATTGGTGTCATTACCTATATTTTATTATGTG
GTTTCCCACCATTCTATGCTTCATCTCAAAACTTATTATTTGAAAAAATTTTAACCGCCG
ATTATGATTTCCCAGAACCAGAATGGACTCATGTCTCTGAACATGCCAAAGCTTTCATTA
GAAACTTAATTGTCAAAGATCCAGATCAACGTCATACTGCCAAACAATGTCTTGATGACC
TTTGGCTCTCTGGTAGTGATCAATCAATTGGTTCTGCTGCTGATCTCCACTCTCATTTCG
CNAAAAGATGAAGAAATATAAGACCAAAGAAGAGGGGTCAATCTTCAAAAATTAAATTAA
TATAATAANA

---ARNVVRQVCAGVEYLHSQGIAHRDLKPENLLCNGEGEDMTIKIADFGLSKIFGTGEA
LETSCGTPDYVAPEVLTGGSYDNAVDMWSIGVITYILLCGFPPFYASSQNLLFEKILTAD
YDFPEPEWTHVSEHAKAFIRNLIVKDPDQRHTAKQCLDDLWLSGSDQSIGSAADLHSHFA
KR*rnirpkkrgqsskikli**

Frame A:
---ARNVVRQVCAGVEYLHSQGIAHRDLKPENLLCNGEGEDMTIKIADFGLSKIFGTGEA
LETSCGTPDYVAPEVLTGGSYDNAVDMWSIGVITYILLCGFPPFYASSQNLLFEKILTAD
YDFPEPEWTHVSEHAKAFIRNLIVKDPDQRHTAKQCLDDLWLSGSDQSIGSAADLHSHFA
KR*rnirpkkrgqsskikli**


Frame B:
---hvml*dkyvlvlnisihkvlliei*nqriyyvmvkvki*qsklqilvlvkylelvkh
wkqvvvhqimlhqkf*lvvhmimqsicgqlvslpifyyvvshhsmlhlktyylkkf*ppi
misqnqnglmslnmpklslet*lskiqinvilpnnvlmtfgslvvinqlvlllistlisx
kdeei*dqrrgvnlqkln*ynx


Frame C:
---t*ccktsmcwc*ispftrycs*rfetrefim*w*r*rydnqncrfws**niwnw*si
gnklwytrlcctrsfnwwfi**csryvvnwchylyfimwfptilcfisklii*knfnrrl
*fprtrmdscl*tcqsfh*klncqrsrstsycqtms**plalw**sinwfcc*splsfrx
kmkkyktkeegsifkn*iniix

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFG501 (VFG501Q) /CSM/VF/VFG5-A/VFG501Q.Seq.d/                       1179   0.0
SFK820 (SFK820Q) /CSM/SF/SFK8-A/SFK820Q.Seq.d/                       1122   0.0
AFL780 (AFL780Q) /CSM/AF/AFL7-D/AFL780Q.Seq.d/                       1120   0.0
SSI101 (SSI101Q) /CSM/SS/SSI1-A/SSI101Q.Seq.d/                       1096   0.0
VFG381 (VFG381Q) /CSM/VF/VFG3-D/VFG381Q.Seq.d/                       1090   0.0
SSC643 (SSC643Q) /CSM/SS/SSC6-B/SSC643Q.Seq.d/                       1080   0.0
SLC252 (SLC252Q) /CSM/SL/SLC2-C/SLC252Q.Seq.d/                       1066   0.0
VFJ379 (VFJ379Q) /CSM/VF/VFJ3-D/VFJ379Q.Seq.d/                       1049   0.0
SFH150 (SFH150Q) /CSM/SF/SFH1-C/SFH150Q.Seq.d/                       1013   0.0
CFB218 (CFB218Q) /CSM/CF/CFB2-A/CFB218Q.Seq.d/                       1001   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.  1045   0.0    4
M64176|M64176.1 D.discoideum myosin light chain kinase (MLCK) mRNA, complete cds.    84   6e-27  3
AZ667950|AZ667950.1 ENTFN88TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   1e-14  3
AB118103|AB118103.1 Entamoeba histolytica gene for hypothetical protein, complete cds, clone:Eh-2109.    52   1e-14  3
AZ545332|AZ545332.1 ENTGA35TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   2e-10  2
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    50   4e-08  9
BH137166|BH137166.1 ENTNR83TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   2e-06  2
AZ672839|AZ672839.1 ENTIX25TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   2e-06  2
CD130804|CD130804.1 MG1-0007T-L270-F12-U.B MG1-0007 Schistosoma mansoni cDNA clone MG1-0007T-L270-F12.B, mRNA sequence.    56   1e-05  2
AU083681|AU083681.1 Cryptomeria japonica mRNA, clone:CC0359, 5' end, expressed in inner bark.    44   2e-05  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q869W6) RecName: Full=Probable myosin light chain kinase DDB_G0...   375   e-103
(Q86AD7) RecName: Full=Probable myosin light chain kinase DDB_G0...   211   2e-53
BC074183_1(BC074183|pid:none) Xenopus laevis MGC82022 protein, m...   191   1e-47
AB076903_1(AB076903|pid:none) Ciona intestinalis Ci-CaM-K mRNA f...   190   2e-47
AK052401_1(AK052401|pid:none) Mus musculus 13 days embryo lung c...   190   3e-47
(Q8IU85) RecName: Full=Calcium/calmodulin-dependent protein kina...   190   3e-47
AY335686_1(AY335686|pid:none) Synthetic construct Homo sapiens C...   190   3e-47
(Q8BW96) RecName: Full=Calcium/calmodulin-dependent protein kina...   190   3e-47
BC110966_1(BC110966|pid:none) Xenopus laevis calcium/calmodulin-...   188   1e-46
BC041721_1(BC041721|pid:none) Xenopus laevis calcium/calmodulin-...   186   4e-46

 psg: 0.72  gvh: 0.34  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.38  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	  4.0 %: vacuolar

>> prediction for VFG501 is cyt

>VFG501Z.Seq
----------TGCACGTAATGTTGTAAGACAAGTATGTGCTGGTGTTGAATATCTCCATT
CACAAGGTATTGCTCATAGAGATTTGAAACCAGAGAATTTATTATGTAATGGTGAAGGTG
AAGATATGACAATCAAAATTGCAGATTTTGGTCTTAGTAAAATATTTGGAACTGGTGAAG
CATTGGAAACAAGTTGTGGTACACCAGATTATGTTGCACCAGAAGTTTTAACTGGTGGTT
CATATGATAATGCAGTCGATATGTGGTCAATTGGTGTCATTACCTATATTTTATTATGTG
GTTTCCCACCATTCTATGCTTCATCTCAAAACTTATTATTTGAAAAAATTTTAACCGCCG
ATTATGATTTCCCAGAACCAGAATGGACTCATGTCTCTGAACATGCCAAAGCTTTCATTA
GAAACTTAATTGTCAAAGATCCAGATCAACGTCATACTGCCAAACAATGTCTTGATGACC
TTTGGCTCTCTGGTAGTGATCAATCAATTGGTTCTGCTGCTGATCTCCACTCTCATTTCG
CNAAAAGATGAAGAAATATAAGACCAAAGAAGAGGGGTCAATCTTCAAAAATTAAATTAA
TATAATAANA