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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM534
Library AF
(Link to library)
Clone ID AFM534
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM534P
(Link to Original site)
Representative DNA sequence 
>AFM534 (AFM534Q) /CSM/AF/AFM5-B/AFM534Q.Seq.d/
AATATTGTTTTACATATAAATAATAATGTTAAAATCAATTTCATTATTAAAAAATAATCA
AATATTATTAAAAAATATAATTAATAATGGTAGAATTATAAATAATGTTGGTGAAAAATT
ATCATCAAAATCATTATTAAAAATTAATTATTCATCATCAACAACTGATAGAACATTTAA
TATTTTAGATGGTACAATTGATAAGAATTCAGCAGAATATAAAGATAATTTAATTAATAT
GAATTCAACATTAAAACAATTAAAAGAAAATATTGAAAAGATTAAATTAGGTGGTGGTGA
AAAATTAAATCAAAAGAATATTTCACGTGGAAAGTTATTAGGTACGTGAACGTATTGAAG
CATTGATTGATGTTGGATCACCATTTTTAGAGTTTTCTCAATTGGCAGGTTGGGGAATGT
ATGGNAAXXXXXXXXXXCGTAAGGTTATCGCACGTTTAGTCGATGGTAGTAGATTCGATG
AATTCAAAGAACTCTATGGCACAACTTTAATTTGTGGTTTTGCACGTGTACATGGTATGC
CAGTTGGTATCATCGCCAACAACGGTATTCTCTTTAGTGAAAGTGCCGTCAAGGGTGCCC
ATTTCATTGAACTTTGCAATCAAAGAGGTATCCCCTTAGTCTTCCTTCAAAACATCACTG
GTTTCATGGTTGGTAAAACTTATGAATCTAAAGGTATAGCCAAGGATGGCGCTAAAATGG
TCATGGCTGTTGCCACCGCCAAAGTTCCAAAGATTACAATGATCATTGGTGGTAGTTTTG
GTGCTGGTAATTATGGTATGTGTGGTCGTTCCTACAGTCCACGTTTCCTTTACATGTGGC
CAAATGCTAAAATCTCTGTTATGGGTGGAGAACAAGCTGCCTCTGTTTTAGCTCAAATTC
AAAAGGATAACATGGCAAAAGAAAATAAACAATGGTCACCAGAAGAAGAAAATACTTTCA
AAAAACCAATCTCTGATAAATTCGAAGAAGAAGGTTCAATCTATTACAGTTCAGCTCGTT
GTTGGGATGATGGTGTTATCGATCCACAAGATTCTCGTAAAGTTATCGCTTTAAGTTTAA
GTGCTTGTAGAATCAACCAATTAATCCNCCATC
sequence update 2009. 4. 3
Translated Amino Acid sequence 
ycftyk**c*nqfhy*kiikyy*ki*limvel*imlvknyhqnhy*kliihhqqliehli
f*mvqliriqqnikii*li*iqh*nn*kkilkrln*vvvkn*ikrifhvesy*VRERIEA
LIDVGSPFLEFSQLAGWGMYG---

---RKVIARLVDGSRFDEFKELYGTTLICGFARVHGMPVGIIANNGILFSESAVKGAHFI
ELCNQRGIPLVFLQNITGFMVGKTYESKGIAKDGAKMVMAVATAKVPKITMIIGGSFGAG
NYGMCGRSYSPRFLYMWPNAKISVMGGEQAASVLAQIQKDNMAKENKQWSPEEENTFKKP
ISDKFEEEGSIYYSSARCWDDGVIDPQDSRKVIALSLSACRINQLIXH
Translated Amino Acid sequence (All Frames) 
Frame A:
nivlhinnnvkinfiikk*sniikkyn**w*nyk*cw*kiiikiiikn*lfiinn**ni*
yfrwyn**efsri*r*fn*yefniktikrky*kd*irww*kikskeyftwkviryvnvlk
h*lmldhhf*sflnwqvgecmx---

---RKVIARLVDGSRFDEFKELYGTTLICGFARVHGMPVGIIANNGILFSESAVKGAHFI
ELCNQRGIPLVFLQNITGFMVGKTYESKGIAKDGAKMVMAVATAKVPKITMIIGGSFGAG
NYGMCGRSYSPRFLYMWPNAKISVMGGEQAASVLAQIQKDNMAKENKQWSPEEENTFKKP
ISDKFEEEGSIYYSSARCWDDGVIDPQDSRKVIALSLSACRINQLIXH

Frame B:
ilfyi*iimlksisllknnqillkniinngriinnvgeklssksllkinysssttdrtfn
ildgtidknsaeykdnlinmnstlkqlkeniekiklgggeklnqknisrgkllgt*ty*s
id*cwitifrvfsigrlgnvwx---

---vrlshv*smvvdsmnsknsmaql*fvvlhvymvcqlvsspttvfslvkvpsrvpisl
nfaikevsp*ssfktslvswlvklmnlkv*prmalkwswllpppkfqrlq*slvvvlvlv
imvcvvvptvhvsftcgqmlksllwvenklplf*lkfkritwqkkinnghqkkkilsknq
slinskkkvqsitvqlvvgmmvlsihkilvklsl*v*vlvestn*sxi

Frame C:
ycftyk**c*nqfhy*kiikyy*ki*limvel*imlvknyhqnhy*kliihhqqliehli
f*mvqliriqqnikii*li*iqh*nn*kkilkrln*vvvkn*ikrifhvesy*VRERIEA
LIDVGSPFLEFSQLAGWGMYG---

---*gyrtfsrw**ir*iqrtlwhnfnlwfctctwyaswyhrqqrysl**kcrqgcpfh*
tlqskryplslpskhhwfhgw*nl*i*rysqgwr*nghgcchrqsskdyndhww*fwcw*
lwyvwsflqstfplhvakc*nlcygwrtsclcfssnskg*hgkrk*tmvtrrrkyfqktn
l**irrrrfnllqfssllg*wcyrstrfs*syrfkfkcl*nqpinpp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM534 (AFM534Q) /CSM/AF/AFM5-B/AFM534Q.Seq.d/                       1503   0.0
VFA387 (VFA387Q) /CSM/VF/VFA3-D/VFA387Q.Seq.d/                       1310   0.0
SLE686 (SLE686Q) /CSM/SL/SLE6-D/SLE686Q.Seq.d/                       1308   0.0
SSI206 (SSI206Q) /CSM/SS/SSI2-A/SSI206Q.Seq.d/                       1158   0.0
VFK423 (VFK423Q) /CSM/VF/VFK4-A/VFK423Q.Seq.d/                       1126   0.0
VSE164 (VSE164Q) /CSM/VS/VSE1-C/VSE164Q.Seq.d/                       1078   0.0
SLE678 (SLE678Q) /CSM/SL/SLE6-D/SLE678Q.Seq.d/                       1003   0.0
VFK325 (VFK325Q) /CSM/VF/VFK3-B/VFK325Q.Seq.d/                        999   0.0
FC-BA21 (FC-BA21Q) /CSM/FC/FC-BA/FC-BA21Q.Seq.d/                      969   0.0
SSJ690 (SSJ690Q) /CSM/SS/SSJ6-D/SSJ690Q.Seq.d/                        944   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ347150) Dictyostelium discoideum cDNA clone:dda26c09, 3' ...  1334   0.0    1
(BJ428978) Dictyostelium discoideum cDNA clone:ddv1n21, 3' e...  1310   0.0    1
(AC115599) Dictyostelium discoideum chromosome 2 map 4229098...  1292   0.0    2
(AU034915) Dictyostelium discoideum slug cDNA, clone SLE686.     1281   0.0    2
(C90323) Dictyostelium discoideum slug cDNA, clone SSI206.       1130   0.0    2
(BJ433238) Dictyostelium discoideum cDNA clone:ddv21n06, 3' ...  1011   0.0    3
(AU262917) Dictyostelium discoideum vegetative cDNA clone:VS...  1051   0.0    2
(BJ433253) Dictyostelium discoideum cDNA clone:ddv21b07, 3' ...   995   0.0    1
(AU034909) Dictyostelium discoideum slug cDNA, clone SLE678.      975   0.0    2
(C25766) Dictyostelium discoideum gamete cDNA, clone FC-BA21.     942   0.0    2
dna update 2009. 4.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q8T2J9) RecName: Full=Methylcrotonoyl-CoA carboxylase beta chai...   415   e-114
AE008334_10(AE008334|pid:none) Agrobacterium tumefaciens str. C5...   328   2e-88
CP000613_374(CP000613|pid:none) Rhodospirillum centenum SW, comp...   318   3e-85
CP000148_3253(CP000148|pid:none) Geobacter metallireducens GS-15...   318   3e-85
BC091016_1(BC091016|pid:none) Xenopus tropicalis methylcrotonoyl...   315   2e-84
CP000089_92(CP000089|pid:none) Dechloromonas aromatica RCB, comp...   315   2e-84
CU633749_120(CU633749|pid:none) Cupriavidus taiwanensis str. LMG...   313   9e-84
CP000449_1730(CP000449|pid:none) Maricaulis maris MCS10, complet...   313   9e-84
CU633749_1505(CU633749|pid:none) Cupriavidus taiwanensis str. LM...   313   9e-84
(Q5XIT9) RecName: Full=Methylcrotonoyl-CoA carboxylase beta chai...   312   1e-83
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM534 is

5' end seq. ID AFM534F
5' end seq. 
>AFM534F.Seq
AATATTGTTTTACATATAAATAATAATGTTAAAATCAATTTCATTATTAAAAAATAATCA
AATATTATTAAAAAATATAATTAATAATGGTAGAATTATAAATAATGTTGGTGAAAAATT
ATCATCAAAATCATTATTAAAAATTAATTATTCATCATCAACAACTGATAGAACATTTAA
TATTTTAGATGGTACAATTGATAAGAATTCAGCAGAATATAAAGATAATTTAATTAATAT
GAATTCAACATTAAAACAATTAAAAGAAAATATTGAAAAGATTAAATTAGGTGGTGGTGA
AAAATTAAATCAAAAGAATATTTCACGTGGAAAGTTATTAGGTACGTGAACGTATTGAAG
CATTGATTGATGTTGGATCACCATTTTTAGAGTTTTCTCAATTGGCAGGTTGGGGAATGT
ATGGNAAXXXXXXXXXX
Length of 5' end seq. 437
3' end seq. ID AFM534Z
3' end seq. 
>AFM534Z.Seq
XXXXXXXXXXCGTAAGGTTATCGCACGTTTAGTCGATGGTAGTAGATTCGATGAATTCAA
AGAACTCTATGGCACAACTTTAATTTGTGGTTTTGCACGTGTACATGGTATGCCAGTTGG
TATCATCGCCAACAACGGTATTCTCTTTAGTGAAAGTGCCGTCAAGGGTGCCCATTTCAT
TGAACTTTGCAATCAAAGAGGTATCCCCTTAGTCTTCCTTCAAAACATCACTGGTTTCAT
GGTTGGTAAAACTTATGAATCTAAAGGTATAGCCAAGGATGGCGCTAAAATGGTCATGGC
TGTTGCCACCGCCAAAGTTCCAAAGATTACAATGATCATTGGTGGTAGTTTTGGTGCTGG
TAATTATGGTATGTGTGGTCGTTCCTACAGTCCACGTTTCCTTTACATGTGGCCAAATGC
TAAAATCTCTGTTATGGGTGGAGAACAAGCTGCCTCTGTTTTAGCTCAAATTCAAAAGGA
TAACATGGCAAAAGAAAATAAACAATGGTCACCAGAAGAAGAAAATACTTTCAAAAAACC
AATCTCTGATAAATTCGAAGAAGAAGGTTCAATCTATTACAGTTCAGCTCGTTGTTGGGA
TGATGGTGTTATCGATCCACAAGATTCTCGTAAAGTTATCGCTTTAAGTTTAAGTGCTTG
TAGAATCAACCAATTAATCCNCCATC
Length of 3' end seq. 686
Connected seq. ID AFM534P
Connected seq. 
>AFM534P.Seq
AATATTGTTTTACATATAAATAATAATGTTAAAATCAATTTCATTATTAAAAAATAATCA
AATATTATTAAAAAATATAATTAATAATGGTAGAATTATAAATAATGTTGGTGAAAAATT
ATCATCAAAATCATTATTAAAAATTAATTATTCATCATCAACAACTGATAGAACATTTAA
TATTTTAGATGGTACAATTGATAAGAATTCAGCAGAATATAAAGATAATTTAATTAATAT
GAATTCAACATTAAAACAATTAAAAGAAAATATTGAAAAGATTAAATTAGGTGGTGGTGA
AAAATTAAATCAAAAGAATATTTCACGTGGAAAGTTATTAGGTACGTGAACGTATTGAAG
CATTGATTGATGTTGGATCACCATTTTTAGAGTTTTCTCAATTGGCAGGTTGGGGAATGT
ATGGNAA----------CGTAAGGTTATCGCACGTTTAGTCGATGGTAGTAGATTCGATG
AATTCAAAGAACTCTATGGCACAACTTTAATTTGTGGTTTTGCACGTGTACATGGTATGC
CAGTTGGTATCATCGCCAACAACGGTATTCTCTTTAGTGAAAGTGCCGTCAAGGGTGCCC
ATTTCATTGAACTTTGCAATCAAAGAGGTATCCCCTTAGTCTTCCTTCAAAACATCACTG
GTTTCATGGTTGGTAAAACTTATGAATCTAAAGGTATAGCCAAGGATGGCGCTAAAATGG
TCATGGCTGTTGCCACCGCCAAAGTTCCAAAGATTACAATGATCATTGGTGGTAGTTTTG
GTGCTGGTAATTATGGTATGTGTGGTCGTTCCTACAGTCCACGTTTCCTTTACATGTGGC
CAAATGCTAAAATCTCTGTTATGGGTGGAGAACAAGCTGCCTCTGTTTTAGCTCAAATTC
AAAAGGATAACATGGCAAAAGAAAATAAACAATGGTCACCAGAAGAAGAAAATACTTTCA
AAAAACCAATCTCTGATAAATTCGAAGAAGAAGGTTCAATCTATTACAGTTCAGCTCGTT
GTTGGGATGATGGTGTTATCGATCCACAAGATTCTCGTAAAGTTATCGCTTTAAGTTTAA
GTGCTTGTAGAATCAACCAATTAATCCNCCATC
Length of connected seq. 1103
Full length Seq ID -
Full length Seq. -
Length of full length seq. -