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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFG381
Library VF
(Link to library)
Clone ID VFG381
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U11996-1
Original site URL
Representative seq. ID VFG381P
(Link to Original site)
Representative DNA sequence 
>VFG381 (VFG381Q) /CSM/VF/VFG3-D/VFG381Q.Seq.d/
AAGATATAAAATTACAGACATTTAGCAATAAATATAAAAGTACCAGTACATTTACTTACA
ACCCTATTTAATAGTAGGGATAGTTAATAATATAAATATAAATAACAACAATAAGAACAA
TAAAAAAAAAAXXXXXXXXXXAAAAGGTGCACGGTAATGTCGTAAGACAAGTATGTGCTG
GTGTTGAATATCTCCATTCACAAGGTATTGCTCATAGAGATTTGAAACCAGAGAATTTAT
TATGTAATGGTGAAGGTGAAGATATGACAATCAAAATTGCAGATTTTGGTCTTAGTAAAA
TATTTGGAACTGGTGAAGCATTGGAAACAAGTTGTGGTACACCAGATTATGTTGCACCAG
AAGTTTTAACTGGTGGTTCATATGATAATGCAGTCGATATGTGGTCAATTGGTGTCATTA
CCTATATTTTATTATGTGGTTTCCCACCATTCTATGCTTCATCTCAAAACTTATTATTTG
AAAAAATTTTAACCGCTGATTATGATTTCCCAGAACCAGAATGGACTCATGTCTCTGAAC
ATGCCAAAGCTTTCATTAGAAACTTAATTGTCAAAGATCCAGATCAACGTCATACTGCCA
AACAATGTCTTGATGACCTTTGGCTCTCTGGTAGTGATCAATCAATTGGTTCTGCTGCTG
ATCTCCACTCTCATTTCGCTGAAAAGAGAAGAAATATAAGACCAAAGAAGAGGGGTCAAT
CTTCAAATAATTAAATTTAATAATAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
rykitdi*q*i*KYQYIYLQPYLIVGIVNNININNNNKNNKKK---

---KVHGNVVRQVCAGVEYLHSQGIAHRDLKPENLLCNGEGEDMTIKIADFGLSKIFGTG
EALETSCGTPDYVAPEVLTGGSYDNAVDMWSIGVITYILLCGFPPFYASSQNLLFEKILT
ADYDFPEPEWTHVSEHAKAFIRNLIVKDPDQRHTAKQCLDDLWLSGSDQSIGSAADLHSH
FAEKRRNIRPKKRGQSSNN*i***
Translated Amino Acid sequence (All Frames) 
Frame A:
ki*nyrhlainikvpvhllttlfnsrds**ykyk*qq*eq*kk---

---krctvms*dkyvlvlnisihkvlliei*nqriyyvmvkvki*qsklqilvlvkylel
vkhwkqvvvhqimlhqkf*lvvhmimqsicgqlvslpifyyvvshhsmlhlktyylkkf*
plimisqnqnglmslnmpklslet*lskiqinvilpnnvlmtfgslvvinqlvlllistl
islkreei*dqrrgvnlqiikfnnn

Frame B:
rykitdi*q*i*KYQYIYLQPYLIVGIVNNININNNNKNNKKK---

---kgar*crktsmcwc*ispftrycs*rfetrefim*w*r*rydnqncrfws**niwnw
*signklwytrlcctrsfnwwfi**csryvvnwchylyfimwfptilcfisklii*knfn
r*l*fprtrmdscl*tcqsfh*klncqrsrstsycqtms**plalw**sinwfcc*spls
fr*kekkyktkeegsifk*lnlii

Frame C:
diklqtfsnkykststftynpi***g*lii*i*ittirtikkk---

---KVHGNVVRQVCAGVEYLHSQGIAHRDLKPENLLCNGEGEDMTIKIADFGLSKIFGTG
EALETSCGTPDYVAPEVLTGGSYDNAVDMWSIGVITYILLCGFPPFYASSQNLLFEKILT
ADYDFPEPEWTHVSEHAKAFIRNLIVKDPDQRHTAKQCLDDLWLSGSDQSIGSAADLHSH
FAEKRRNIRPKKRGQSSNN*i***
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFG381 (VFG381Q) /CSM/VF/VFG3-D/VFG381Q.Seq.d/                       1201   0.0
SFK820 (SFK820Q) /CSM/SF/SFK8-A/SFK820Q.Seq.d/                       1142   0.0
SSI101 (SSI101Q) /CSM/SS/SSI1-A/SSI101Q.Seq.d/                       1136   0.0
SLC252 (SLC252Q) /CSM/SL/SLC2-C/SLC252Q.Seq.d/                       1106   0.0
AFL780 (AFL780Q) /CSM/AF/AFL7-D/AFL780Q.Seq.d/                       1104   0.0
VFG501 (VFG501Q) /CSM/VF/VFG5-A/VFG501Q.Seq.d/                       1090   0.0
SSC643 (SSC643Q) /CSM/SS/SSC6-B/SSC643Q.Seq.d/                       1084   0.0
VFJ379 (VFJ379Q) /CSM/VF/VFJ3-D/VFJ379Q.Seq.d/                       1053   0.0
SFH150 (SFH150Q) /CSM/SF/SFH1-C/SFH150Q.Seq.d/                       1011   0.0
CFB218 (CFB218Q) /CSM/CF/CFB2-A/CFB218Q.Seq.d/                        999   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.  1051  0.0  4
M64176|M64176.1 D.discoideum myosin light chain kinase (MLCK) mRNA, complete cds.    84  5e-24  3
AB118103|AB118103.1 Entamoeba histolytica gene for hypothetical protein, complete cds, clone:Eh-2109.    52  8e-17  4
AZ667950|AZ667950.1 ENTFN88TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52  9e-15  3
AZ545332|AZ545332.1 ENTGA35TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52  8e-11  3
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    50  1e-08  10
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    56  3e-07  12
BH137166|BH137166.1 ENTNR83TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52  1e-06  2
AZ672839|AZ672839.1 ENTIX25TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52  1e-06  2
CD130804|CD130804.1 MG1-0007T-L270-F12-U.B MG1-0007 Schistosoma mansoni cDNA clone MG1-0007T-L270-F12.B, mRNA sequence.    56  8e-06  2
dna update 2003. 9.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q869W6) RecName: Full=Probable myosin light chain kinase DDB_G0...   380   e-104
(Q86AD7) RecName: Full=Probable myosin light chain kinase DDB_G0...   213   4e-54
AB076903_1(AB076903|pid:none) Ciona intestinalis Ci-CaM-K mRNA f...   191   2e-47
BC074183_1(BC074183|pid:none) Xenopus laevis MGC82022 protein, m...   191   2e-47
AK052401_1(AK052401|pid:none) Mus musculus 13 days embryo lung c...   190   5e-47
(Q8IU85) RecName: Full=Calcium/calmodulin-dependent protein kina...   190   5e-47
(Q8BW96) RecName: Full=Calcium/calmodulin-dependent protein kina...   190   5e-47
AY335686_1(AY335686|pid:none) Synthetic construct Homo sapiens C...   189   8e-47
BC110966_1(BC110966|pid:none) Xenopus laevis calcium/calmodulin-...   188   1e-46
BC041721_1(BC041721|pid:none) Xenopus laevis calcium/calmodulin-...   186   5e-46
protein update 2009. 6.20
PSORT 

 psg: 0.90  gvh: 0.33  alm: 0.34  top: 0.83  tms: 0.00  mit: 0.22  mip: 0.07
 nuc: 0.16  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial

>> prediction for VFG381 is nuc
5' end seq. ID VFG381F
5' end seq. 
>VFG381F.Seq
AAGATATAAAATTACAGACATTTAGCAATAAATATAAAAGTACCAGTACATTTACTTACA
ACCCTATTTAATAGTAGGGATAGTTAATAATATAAATATAAATAACAACAATAAGAACAA
TAAAAAAAAAA----------
Length of 5' end seq. 131
3' end seq. ID VFG381Z
3' end seq. 
>VFG381Z.Seq
----------AAAAGGTGCACGGTAATGTCGTAAGACAAGTATGTGCTGGTGTTGAATAT
CTCCATTCACAAGGTATTGCTCATAGAGATTTGAAACCAGAGAATTTATTATGTAATGGT
GAAGGTGAAGATATGACAATCAAAATTGCAGATTTTGGTCTTAGTAAAATATTTGGAACT
GGTGAAGCATTGGAAACAAGTTGTGGTACACCAGATTATGTTGCACCAGAAGTTTTAACT
GGTGGTTCATATGATAATGCAGTCGATATGTGGTCAATTGGTGTCATTACCTATATTTTA
TTATGTGGTTTCCCACCATTCTATGCTTCATCTCAAAACTTATTATTTGAAAAAATTTTA
ACCGCTGATTATGATTTCCCAGAACCAGAATGGACTCATGTCTCTGAACATGCCAAAGCT
TTCATTAGAAACTTAATTGTCAAAGATCCAGATCAACGTCATACTGCCAAACAATGTCTT
GATGACCTTTGGCTCTCTGGTAGTGATCAATCAATTGGTTCTGCTGCTGATCTCCACTCT
CATTTCGCTGAAAAGAGAAGAAATATAAGACCAAAGAAGAGGGGTCAATCTTCAAATAAT
TAAATTTAATAATAAT
Length of 3' end seq. 606
Connected seq. ID VFG381P
Connected seq. 
>VFG381P.Seq
AAGATATAAAATTACAGACATTTAGCAATAAATATAAAAGTACCAGTACATTTACTTACA
ACCCTATTTAATAGTAGGGATAGTTAATAATATAAATATAAATAACAACAATAAGAACAA
TAAAAAAAAAA----------AAAAGGTGCACGGTAATGTCGTAAGACAAGTATGTGCTG
GTGTTGAATATCTCCATTCACAAGGTATTGCTCATAGAGATTTGAAACCAGAGAATTTAT
TATGTAATGGTGAAGGTGAAGATATGACAATCAAAATTGCAGATTTTGGTCTTAGTAAAA
TATTTGGAACTGGTGAAGCATTGGAAACAAGTTGTGGTACACCAGATTATGTTGCACCAG
AAGTTTTAACTGGTGGTTCATATGATAATGCAGTCGATATGTGGTCAATTGGTGTCATTA
CCTATATTTTATTATGTGGTTTCCCACCATTCTATGCTTCATCTCAAAACTTATTATTTG
AAAAAATTTTAACCGCTGATTATGATTTCCCAGAACCAGAATGGACTCATGTCTCTGAAC
ATGCCAAAGCTTTCATTAGAAACTTAATTGTCAAAGATCCAGATCAACGTCATACTGCCA
AACAATGTCTTGATGACCTTTGGCTCTCTGGTAGTGATCAATCAATTGGTTCTGCTGCTG
ATCTCCACTCTCATTTCGCTGAAAAGAGAAGAAATATAAGACCAAAGAAGAGGGGTCAAT
CTTCAAATAATTAAATTTAATAATAAT
Length of connected seq. 737
Full length Seq ID -
Full length Seq. -
Length of full length seq. -