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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFG365
Library VF
(Link to library)
Clone ID VFG365
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12898-1
Original site URL
Representative seq. ID VFG365E
(Link to Original site)
Representative DNA sequence 
>VFG365 (VFG365Q) /CSM/VF/VFG3-C/VFG365Q.Seq.d/
AACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTAGAGTTTATGTTGATG
GTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGACAAGCAAGAGAATTAG
GAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAAAAAATAAAGGTCCAC
CAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTAAATGGGCAGATGAAG
TTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATAGTTTAAACTGTGATT
TTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAGATGTTTATGAAGGCA
TTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTGTTTCAACAACCGAGT
TGGTTGGTCGTATGTTATTGTGCACCAAAGACCATCTTCAAAATGTTTCAGGTGAGCAAA
CCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAATCACCATACACAAGTT
TATCACATTTCCTCCCAACCACCAGAAAGATCGTCCAATTCTCAGAAGGTCGTTCACCAA
AACCAAACGATAAAATCATTTATATGGATGGTGGTTTCGATTTATTCCATGTAGGTCATA
CTGAGGCTTTGAAACAAGCAAGAGCACTCGGTGACTATCTCATCGTTGGTGTTCATGATG
ATAGAGTAGTTCACGAACAAAAAGGTAGCAACTTCCCAATTATGAATTTACATGAACGTG
TACTCTCTGTACTCTCATGTCGTTATGTCGATGAAGTTGTCATTGGTGCTCCATTCAGTG
TCACCAAAGATATGATTGACTCACTTCACATCAATGTTGTCGTTCATGGTGATGATCAAG
TTGTACTCGGTCCAGAAGGTGGTGTAGATCCATACAAACTCCCAAGAGAATTAGGTATTT
ACAAAGAAGTTAAACATACTGAAGGTCTCACTGCAACTGAAATCGTTAAAAGAATCATTG
ATAATAGACTTCAATATGAAGCTCGTAATCGTAAAAAAGAAGCAAAAGAAATTAATTTCA
TTNAACAATCAAATTAAAAAAAATTAAAAAATAAAAAATAAAAATAAAACAA
sequence update 2001. 6. 9
Translated Amino Acid sequence 
QVFTMSTTTNKKPIRVYVDGCFDLMHFGHANALRQARELGDILVVGVHTDEEIAKNKGPP
VMNEQERYKAVRACKWADEVAEGAPYTLTEEYLDSLNCDFCVHGEDISVGADGKDVYEGI
KKSGKFRFIKRTEGVSTTELVGRMLLCTKDHLQNVSGEQTSPLGGVNPNVLHKQSPYTSL
SHFLPTTRKIVQFSEGRSPKPNDKIIYMDGGFDLFHVGHTEALKQARALGDYLIVGVHDD
RVVHEQKGSNFPIMNLHERVLSVLSCRYVDEVVIGAPFSVTKDMIDSLHINVVVHGDDQV
VLGPEGGVDPYKLPRELGIYKEVKHTEGLTATEIVKRIIDNRLQYEARNRKKEAKEINFI
XQSN*kklknkk*k*n
Translated Amino Acid sequence (All Frames) 
Frame A:
nkflq*vlqliksqlefmlmvvli*cildmqmh*dkqen*eif**lvyiqmkklqkikvh
ql*mnkkdikqyvhvngqmklqkvhlil*lkniwiv*tvifvymvkifqlvqmvkmfmka
lkrvvnldssnvlkvfqqpswlvvcycapktifkmfqvskphhwevsiqtfytnnhhtqv
yhissqpperssnsqkvvhqnqtiksfiwmvvsiysm*vilrl*nkqehsvtisslvfmm
ie*ftnkkvatsql*iymnvyslyshvvmsmklslvlhsvspki*lthftsmlsfmvmik
lysvqkvv*ihtnsqen*vftkklnilkvslqlkslkesliidfnmklvivkkkqkklis
xnnqikkn*kiknknkt


Frame B:
tsfyneyyn**kan*slc*wlf*fnafwtckciktskrirryfsswctyr*rnckk*rst
sye*trki*sstcm*mgr*scrrctlyfn*rifg*fkl*flctw*ryfswcrw*rcl*rh
*kew*i*ihqty*rcfnnrvgwsyvivhqrpsskcfr*anltigrcqskrftqtitihkf
itfppnhqkdrpilrrsftktkr*nhlygwwfrfipcrsy*gfetskstr*lshrwcs**
*sssrtkr*qlpnyeft*tctlctlmslcr*schwcsiqchqryd*ltshqccrsw**ss
ctrsrrwcrsiqtpkrirylqrs*ty*rshcn*nr*knh***tsi*ss*s*krskrn*fh
xtiklkkikk*kikikq


Frame C:
QVFTMSTTTNKKPIRVYVDGCFDLMHFGHANALRQARELGDILVVGVHTDEEIAKNKGPP
VMNEQERYKAVRACKWADEVAEGAPYTLTEEYLDSLNCDFCVHGEDISVGADGKDVYEGI
KKSGKFRFIKRTEGVSTTELVGRMLLCTKDHLQNVSGEQTSPLGGVNPNVLHKQSPYTSL
SHFLPTTRKIVQFSEGRSPKPNDKIIYMDGGFDLFHVGHTEALKQARALGDYLIVGVHDD
RVVHEQKGSNFPIMNLHERVLSVLSCRYVDEVVIGAPFSVTKDMIDSLHINVVVHGDDQV
VLGPEGGVDPYKLPRELGIYKEVKHTEGLTATEIVKRIIDNRLQYEARNRKKEAKEINFI
XQSN*kklknkk*k*n
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFG365 (VFG365Q) /CSM/VF/VFG3-C/VFG365Q.Seq.d/                       2165   0.0
VFN722 (VFN722Q) /CSM/VF/VFN7-A/VFN722Q.Seq.d/                       2147   0.0
VFI626 (VFI626Q) /CSM/VF/VFI6-B/VFI626Q.Seq.d/                       2119   0.0
CFG421 (CFG421Q) /CSM/CF/CFG4-A/CFG421Q.Seq.d/                       2103   0.0
VFJ833 (VFJ833Q) /CSM/VF/VFJ8-B/VFJ833Q.Seq.d/                       2076   0.0
VFA266 (VFA266Q) /CSM/VF/VFA2-C/VFA266Q.Seq.d/                       2046   0.0
VFE318 (VFE318Q) /CSM/VF/VFE3-A/VFE318Q.Seq.d/                       2006   0.0
VFM413 (VFM413Q) /CSM/VF/VFM4-A/VFM413Q.Seq.d/                       1994   0.0
VFG517 (VFG517Q) /CSM/VF/VFG5-A/VFG517Q.Seq.d/                       1994   0.0
VFF530 (VFF530Q) /CSM/VF/VFF5-B/VFF530Q.Seq.d/                       1830   0.0
own update 2001.12. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BG140383|BG140383.1 EST480825 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP17G1 5' sequence, mRNA sequence.    92  2e-16  3
BG131085|BG131085.1 EST463977 tomato crown gall Lycopersicon esculentum cDNA clone cTOE2D11 5' sequence, mRNA sequence.    92  4e-16  3
AW217896|AW217896.1 EST296610 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD1C15, mRNA sequence.    92  3e-15  2
AI778006|AI778006.1 EST258885 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES3N17, mRNA sequence.    92  3e-15  2
AW217261|AW217261.1 EST295975 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC89G22, mRNA sequence.    92  3e-15  2
AW219042|AW219042.1 EST301524 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX2H3, mRNA sequence.    92  4e-15  2
BG138134|BG138134.1 EST478576 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP8K18 5' sequence, mRNA sequence.    92  4e-15  2
BG642411|BG642411.1 EST355887 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD11C15 5' end, mRNA sequence.    92  4e-15  2
BG601025|BG601025.1 EST505920 cSTS Solanum tuberosum cDNA clone cSTS30D8 5' sequence, mRNA sequence.    90  2e-14  2
BG133537|BG133537.1 EST466525 tomato crown gall Lycopersicon esculentum cDNA clone cTOE13N15 5' sequence, mRNA sequence.    92  2e-14  1
dna update 2003. 9.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55BZ4) RecName: Full=Ethanolamine-phosphate cytidylyltransfera...   728   0.0
AE014134_2379(AE014134|pid:none) Drosophila melanogaster chromos...   390   e-107
BT077764_1(BT077764|pid:none) Lepeophtheirus salmonis Pacific fo...   387   e-106
(Q5EA75) RecName: Full=Ethanolamine-phosphate cytidylyltransfera...   385   e-105
AK166319_1(AK166319|pid:none) Mus musculus mammary gland RCB-052...   385   e-105
AL663030_9(AL663030|pid:none) Mouse DNA sequence from clone RP23...   385   e-105
AY189524_1(AY189524|pid:none) Mus musculus CTP:ethanolaminephosp...   379   e-103
AM502250_130(AM502250|pid:none) Leishmania infantum chromosome 32.    377   e-103
BC088018_1(BC088018|pid:none) Xenopus tropicalis phosphate cytid...   374   e-102
AM494969_98(AM494969|pid:none) Leishmania braziliensis chromosom...   374   e-102
protein update 2009. 7.10
PSORT 

 psg: 0.52  gvh: 0.53  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.04
 nuc: 0.08  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFG365 is nuc
5' end seq. ID VFG365F
5' end seq. 
>VFG365F.Seq
AACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTAGAGTTTATGTTGATG
GTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGACAAGCAAGAGAATTAG
GAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAAAAAATAAAGGTCCAC
CAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTAAATGGGCAGATGAAG
TTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATAGTTTAAACTGTGATT
TTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAGATGTTTATGAAGGCA
TTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTGTTTCAACAACCGAGT
TGGTTGGTCGTATGTTATTGTGCACCAAAGAC----------
Length of 5' end seq. 452
3' end seq. ID VFG365Z
3' end seq. 
>VFG365Z.Seq
----------TCAAACGTACTGAAGGTGTTTCAACAACCGAGTTGGTTGGTCGTATGTTA
TTGTGCACCAAAGACCATCTTCAAAATGTTTCAGGTGAGCAAACCTCACCATTGGGAGGT
GTCAATCCAAACGTTTTACACAAACAATCACCATACACAAGTTTATCACATTTCCTCCCA
ACCACCAGAAAGATCGTCCAATTCTCAGAAGGTCGTTCACCAAAACCAAACGATAAAATC
ATTTATATGGATGGTGGTTTCGATTTATTCCATGTAGGTCATACTGAGGCTTTGAAACAA
GCAAGAGCACTCGGTGACTATCTCATCGTTGGTGTTCATGATGATAGAGTAGTTCACGAA
CAAAAAGGTAGCAACTTCCCAATTATGAATTTACATGAACGTGTACTCTCTGTACTCTCA
TGTCGTTATGTCGATGAAGTTGTCATTGGTGCTCCATTCAGTGTCACCAAAGATATGATT
GACTCACTTCACATCAATGTTGTCGTTCATGGTGATGATCAAGTTGTACTCGGTCCAGAA
GGTGGTGTAGATCCATACAAACTCCCAAGAGAATTAGGTATTTACAAAGAAGTTAAACAT
ACTGAAGGTCTCACTGCAACTGAAATCGTTAAAAGAATCATTGATAATAGACTTCAATAT
GAAGCTCGTAATCGTAAAAAAGAAGCAAAAGAAATTAATTTCATTNAACAATCAAATTAA
AAAAAATTAAAAAATAAAAAATAAAAATAAAACAA
Length of 3' end seq. 745
Connected seq. ID VFG365P
Connected seq. 
>VFG365P.Seq
AACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTAGAGTTTATGTTGATG
GTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGACAAGCAAGAGAATTAG
GAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAAAAAATAAAGGTCCAC
CAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTAAATGGGCAGATGAAG
TTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATAGTTTAAACTGTGATT
TTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAGATGTTTATGAAGGCA
TTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTGTTTCAACAACCGAGT
TGGTTGGTCGTATGTTATTGTGCACCAAAGAC----------TCAAACGTACTGAAGGTG
TTTCAACAACCGAGTTGGTTGGTCGTATGTTATTGTGCACCAAAGACCATCTTCAAAATG
TTTCAGGTGAGCAAACCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAAT
CACCATACACAAGTTTATCACATTTCCTCCCAACCACCAGAAAGATCGTCCAATTCTCAG
AAGGTCGTTCACCAAAACCAAACGATAAAATCATTTATATGGATGGTGGTTTCGATTTAT
TCCATGTAGGTCATACTGAGGCTTTGAAACAAGCAAGAGCACTCGGTGACTATCTCATCG
TTGGTGTTCATGATGATAGAGTAGTTCACGAACAAAAAGGTAGCAACTTCCCAATTATGA
ATTTACATGAACGTGTACTCTCTGTACTCTCATGTCGTTATGTCGATGAAGTTGTCATTG
GTGCTCCATTCAGTGTCACCAAAGATATGATTGACTCACTTCACATCAATGTTGTCGTTC
ATGGTGATGATCAAGTTGTACTCGGTCCAGAAGGTGGTGTAGATCCATACAAACTCCCAA
GAGAATTAGGTATTTACAAAGAAGTTAAACATACTGAAGGTCTCACTGCAACTGAAATCG
TTAAAAGAATCATTGATAATAGACTTCAATATGAAGCTCGTAATCGTAAAAAAGAAGCAA
AAGAAATTAATTTCATTNAACAATCAAATTAAAAAAAATTAAAAAATAAAAAATAAAAAT
AAAACAA
Length of connected seq. 1197
Full length Seq ID VFG365E
Full length Seq. 
>VFG365E.Seq
AACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTAGAGTTTATGTTGATG
GTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGACAAGCAAGAGAATTAG
GAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAAAAAATAAAGGTCCAC
CAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTAAATGGGCAGATGAAG
TTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATAGTTTAAACTGTGATT
TTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAGATGTTTATGAAGGCA
TTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTGTTTCAACAACCGAGT
TGGTTGGTCGTATGTTATTGTGCACCAAAGACCATCTTCAAAATGTTTCAGGTGAGCAAA
CCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAATCACCATACACAAGTT
TATCACATTTCCTCCCAACCACCAGAAAGATCGTCCAATTCTCAGAAGGTCGTTCACCAA
AACCAAACGATAAAATCATTTATATGGATGGTGGTTTCGATTTATTCCATGTAGGTCATA
CTGAGGCTTTGAAACAAGCAAGAGCACTCGGTGACTATCTCATCGTTGGTGTTCATGATG
ATAGAGTAGTTCACGAACAAAAAGGTAGCAACTTCCCAATTATGAATTTACATGAACGTG
TACTCTCTGTACTCTCATGTCGTTATGTCGATGAAGTTGTCATTGGTGCTCCATTCAGTG
TCACCAAAGATATGATTGACTCACTTCACATCAATGTTGTCGTTCATGGTGATGATCAAG
TTGTACTCGGTCCAGAAGGTGGTGTAGATCCATACAAACTCCCAAGAGAATTAGGTATTT
ACAAAGAAGTTAAACATACTGAAGGTCTCACTGCAACTGAAATCGTTAAAAGAATCATTG
ATAATAGACTTCAATATGAAGCTCGTAATCGTAAAAAAGAAGCAAAAGAAATTAATTTCA
TTNAACAATCAAATTAAAAAAAATTAAAAAATAAAAAATAAAAATAAAACAA
Length of full length seq. 1132