>VFG251 (VFG251Q) /CSM/VF/VFG2-C/VFG251Q.Seq.d/
XXXXXXXXXXAAATGTATCAGCTGAAGATAAATCANCTGGTAACAAACAAAAGATTACAA
TTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAG
AGAAATTCAAACAACAAGATGAACAACAAAAAGATNGTGTTGAATCAAAGAATAANTTGG
AAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTT
CAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAAT
CAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTG
TCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGC
CAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATT
GACGGAATTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTT
AGTAAAATCAATAAAGCTTNCTCATATATA

---NVSAEDKSXGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDXVESKNXLE
NYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVV
NPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kiis*
*nq*sxliy

Frame A:
---NVSAEDKSXGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDXVESKNXLE
NYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVV
NPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kiis*
*nq*sxliy


Frame B:
---myqlkinxlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnnkkxvlnqrixwk
imhsllkiqlkmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*kp*kqls
iqs*vnsikkevchkvvvcqvvcqmihqrvqttk*tn*idgifqrktslsnrflkk**vs
kinkaxsyi


Frame C:
---cis*r*ixw*qtkdynyq**rssfkrrn*kngc*creiqttr*ttkrxc*ike*xgk
lcihc*kfn*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*deslrsscq
snhe*tlsrrryatrwwyarwyvk*ftkefkqqsrrirlteffrgkhlyqidflknnklv
ksiklxhi

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP873 (VHP873Q) /CSM/VH/VHP8-D/VHP873Q.Seq.d/                       1086   0.0
VHP627 (VHP627Q) /CSM/VH/VHP6-B/VHP627Q.Seq.d/                       1086   0.0
VHO655 (VHO655Q) /CSM/VH/VHO6-C/VHO655Q.Seq.d/                       1086   0.0
VHO461 (VHO461Q) /CSM/VH/VHO4-C/VHO461Q.Seq.d/                       1086   0.0
VHK415 (VHK415Q) /CSM/VH/VHK4-A/VHK415Q.Seq.d/                       1086   0.0
VHJ275 (VHJ275Q) /CSM/VH/VHJ2-D/VHJ275Q.Seq.d/                       1086   0.0
VHJ108 (VHJ108Q) /CSM/VH/VHJ1-A/VHJ108Q.Seq.d/                       1086   0.0
VHI336 (VHI336Q) /CSM/VH/VHI3-B/VHI336Q.Seq.d/                       1086   0.0
VHH806 (VHH806Q) /CSM/VH/VHH8-A/VHH806Q.Seq.d/                       1086   0.0
VHH558 (VHH558Q) /CSM/VH/VHH5-C/VHH558Q.Seq.d/                       1086   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1070  0.0  1
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.  1063  0.0  1
M84019|M84019.1 Hydra magnipapillata heat shock protein 70.1 (hsp70.1) gene, partial cds.    58  1e-11  2
AI218785|AI218785.1 SWOvAFCAP12C05SK Onchocerca volvulus adult female cDNA (SAW98MLW-OvAF) Onchocerca volvulus cDNA clone SWOvAFCAP12C05 5', mRNA sequence.    44  3e-10  3
AI239270|AI239270.1 SWOvAFCAP13G08SK Onchocerca volvulus adult female cDNA (SAW98MLW-OvAF) Onchocerca volvulus cDNA clone SWOvAFCAP13G08 5', mRNA sequence.    44  3e-10  3
AF221542|AF221542.1 Cryptosporidium muris isolate 589 heat shock protein 70 (HSP70) gene, partial cds.    56  2e-09  3
AF221541|AF221541.1 Cryptosporidium serpentis heat shock protein 70 (HSP70) gene, partial cds.    52  2e-09  3
BM162567|BM162567.1 EST565090 PyBS Plasmodium yoelii yoelii cDNA clone PYCKU24 5' end, mRNA sequence.    40  3e-09  4
BM159459|BM159459.1 EST561982 PyBS Plasmodium yoelii yoelii cDNA clone PYCJE40 5' end, mRNA sequence.    40  1e-08  4
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.          64  1e-07  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   228   7e-59
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   228   7e-59
AF033852_1(AF033852|pid:none) Spinacia oleracea cytosolic heat s...   149   5e-35
FB790108_1(FB790108|pid:none) Sequence 9381 from Patent WO200803...   148   9e-35
(P24629) RecName: Full=Heat shock cognate 70 kDa protein 1;  &FB...   146   3e-34
AY253326_1(AY253326|pid:none) Nicotiana tabacum heat shock prote...   145   7e-34
(P22953) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   144   1e-33
FB790106_1(FB790106|pid:none) Sequence 9379 from Patent WO200803...   144   1e-33
S46302(S46302;S41402;JA0169;S36623) dnaK-type molecular chaperon...   144   1e-33
AY035123_1(AY035123|pid:none) Arabidopsis thaliana putative dnaK...   144   1e-33

 psg: 0.75  gvh: 0.08  alm: 0.55  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 24.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for VFG251 is nuc

>VFG251Z.Seq
----------AAATGTATCAGCTGAAGATAAATCANCTGGTAACAAACAAAAGATTACAA
TTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAG
AGAAATTCAAACAACAAGATGAACAACAAAAAGATNGTGTTGAATCAAAGAATAANTTGG
AAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTT
CAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAAT
CAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTG
TCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGC
CAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATT
GACGGAATTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTT
AGTAAAATCAATAAAGCTTNCTCATATATA