NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM520
Library AF
(Link to library)
Clone ID AFM520
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM520P
(Link to Original site)
Representative DNA sequence 
>AFM520 (AFM520Q) /CSM/AF/AFM5-A/AFM520Q.Seq.d/
ATTCATTCAATTTTGTAATTATAAAAAAAGGAATCAAATAATTATATATATATATAAAAA
TATAAAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTATTTTTAATTACATTA
TATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAAAGTCATCGTTCAGAA
TATACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTATCATGTTGTGGTAAG
AAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGATGATTTATCATGTGCT
AAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGTAAACCACATAGAGAT
TTTGTACCATATCATTCACCAAATACAACCACATGTGAATCATTAGGTTGTGAAGCAAGA
GGTATGGAATGTGAATGGGTTGAATCATCACCATGTTATGGAACTTCTTGTTGCCCAAGA
ATTCCAAGATGTGTTGGTCATCATGGTGGCGGTCATAAATGTGACAGAATGAGATGTCCA
GAAGGATTCTATTGTGAAGAACAAGGTGGTAGTGCATGCTGCGTACCACATCATGACGGT
TGXXXXXXXXXXATTAGCACCAATATCGTGAAATGTTCACCAAAGAATGACGAATGTAAA
TGGCATCGTTGTCCACCAGGTTCCAGCTGCTTCAATAGTAGAAACGGTCCACACTGTCTT
GCCAATAATGTATTCCCACAACTTTGTAAAGTTACTCAATGTCCAACTGATTTCTCTTGT
AAAATGATTAGAGGTAATCCAACTTGTATTAAAGCAAGACCACCGGTACCACCACCACAT
TGCTCAACTTGTGCAGAGCTATCATCAGCATGTAATCACGTTGGAATGATTTGTATTCAA
GTACCAAGCAATTGTACCAATACTAGATTCCCATGTTGCCCATCTCATCCAATTTGTATT
CATCCATCAACTACTGCTGCTTCAACCATTGCAACAACTGCATCAACTGTCGCAACTACA
ACCTCTGCAACTACTGCAGGTACAACAACTGGTGGAACTACAACTGGTGGTTCAACTTCT
GATAGTAGTGCTGCTTCCTCAGCCGATAGTAGCGCTGCCTCCTCATCACCATCAAGCAGT
GCTGCTTCAAGTGCCGCTTCAAGTGAACCATCAAGTAGCGCCGCTTCAAGTAGTGCTCCA
TCCTCTGCTTCAAGTAGCGCTCCATCCTCTGCCCCAAGTAGTGCTCCATCATCATCAGCT
TCAAGTTCAGCTGCATCATCAGCTGCAAGNCTCAGAAAGNCTCAGAAAGCTCATCAGCCA
CTTCATAAATAAA
sequence update 2009. 4. 3
Translated Amino Acid sequence 
ihsil*l*kkesnnyiyi*KYKKNKMRILYLASLLFLITLYLSPTFGWGGGRDCESHRSE
YTCKSDRSCAYLPFVSCCGKKEFFCVNRDHRNCCDDLSCAKNTRTGEIFEIWSSCKPHRD
FVPYHSPNTTTCESLGCEARGMECEWVESSPCYGTSCCPRIPRCVGHHGGGHKCDRMRCP
EGFYCEEQGGSACCVPHHDG---

---ISTNIVKCSPKNDECKWHRCPPGSSCFNSRNGPHCLANNVFPQLCKVTQCPTDFSCK
MIRGNPTCIKARPPVPPPHCSTCAELSSACNHVGMICIQVPSNCTNTRFPCCPSHPICIH
PSTTAASTIATTASTVATTTSATTAGTTTGGTTTGGSTSDSSAASSADSSAASSSPSSSA
ASSAASSEPSSSAASSSAPSSASSSAPSSAPSSAPSSSASSSAASSAAXLRKXQKAHQPL
HK*
Translated Amino Acid sequence (All Frames) 
Frame A:
ihsil*l*kkesnnyiyi*KYKKNKMRILYLASLLFLITLYLSPTFGWGGGRDCESHRSE
YTCKSDRSCAYLPFVSCCGKKEFFCVNRDHRNCCDDLSCAKNTRTGEIFEIWSSCKPHRD
FVPYHSPNTTTCESLGCEARGMECEWVESSPCYGTSCCPRIPRCVGHHGGGHKCDRMRCP
EGFYCEEQGGSACCVPHHDG---

---ISTNIVKCSPKNDECKWHRCPPGSSCFNSRNGPHCLANNVFPQLCKVTQCPTDFSCK
MIRGNPTCIKARPPVPPPHCSTCAELSSACNHVGMICIQVPSNCTNTRFPCCPSHPICIH
PSTTAASTIATTASTVATTTSATTAGTTTGGTTTGGSTSDSSAASSADSSAASSSPSSSA
ASSAASSEPSSSAASSSAPSSASSSAPSSAPSSAPSSSASSSAASSAAXLRKXQKAHQPL
HK*

Frame B:
fiqfcnykkrnqiiiyiyknikkik*efyi*llfyf*lhyiyhqllvgvvaeivkvivqn
ihvnqievvhiyhlyhvvvrknfsvliviieivvmiyhvlktrelvkslrfgqvvnhiei
lyhiihqiqphvnh*vvkqevwnvnglnhhhvmellvaqefqdvlvimvavinvte*dvq
kdsivknkvvvhaayhimtv---

---lapis*nvhqrmtnvngivvhqvpaasivetvhtvlpimyshnfvkllnvqlislvk
*leviqlvlkqdhryhhhiaqlvqsyhqhvitle*fvfkyqaivpildshvahliqfvfi
hqllllqplqqlhqlsqlqplqllqvqqlvelqlvvqllivvllpqpivalpphhhqavl
lqvplqvnhqvaplqvvlhpllqvalhplpqvvlhhhqlqvqlhhqlqxsexlrklishf
ink

Frame C:
sfnfviikkgik*lyiyiki*kk*nenfifsfsfifnyiisitnfwlgwwqrl*kssfri
ym*ir*klcifticimlw*eriflc*s*s*kll**fimc*khenw*nl*dlvkl*tt*rf
ctisftkynhm*iirl*skrygm*mg*iitmlwnfllpknskmcwsswwrs*m*qnemsr
rill*rtrw*cmlrtts*rl---

---*hqyremftke*rm*maslstrfqllq**krstlscq*cipttl*sysmsn*fll*n
d*r*snly*skttgttttllnlcraiism*srwndlysstkqlyqy*ipmlpissnlyss
inyccfnhcnncincrnynlcnycrynnwwnynwwfnf***ccflsr**rcllitikqcc
fkcrfk*tik*rrfk*csilcfk*rsilcpk*csiiisfkfsciisckxqkxsesssats
*i
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM520 (AFM520Q) /CSM/AF/AFM5-A/AFM520Q.Seq.d/                       2430   0.0
AFM271 (AFM271Q) /CSM/AF/AFM2-C/AFM271Q.Seq.d/                       1409   0.0
CHQ574 (CHQ574Q) /CSM/CH/CHQ5-D/CHQ574Q.Seq.d/                       1405   0.0
CFH404 (CFH404Q) /CSM/CF/CFH4-A/CFH404Q.Seq.d/                       1402   0.0
CFJ209 (CFJ209Q) /CSM/CF/CFJ2-A/CFJ209Q.Seq.d/                       1400   0.0
CFJ121 (CFJ121Q) /CSM/CF/CFJ1-A/CFJ121Q.Seq.d/                       1400   0.0
CFH370 (CFH370Q) /CSM/CF/CFH3-C/CFH370Q.Seq.d/                       1400   0.0
CFH292 (CFH292Q) /CSM/CF/CFH2-D/CFH292Q.Seq.d/                       1400   0.0
AFO728 (AFO728Q) /CSM/AF/AFO7-B/AFO728Q.Seq.d/                       1400   0.0
AFN578 (AFN578Q) /CSM/AF/AFN5-D/AFN578Q.Seq.d/                       1400   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ347090) Dictyostelium discoideum cDNA clone:dda26g05, 3' ...  1417   0.0    1
(BJ346959) Dictyostelium discoideum cDNA clone:dda25m18, 3' ...  1409   0.0    1
(BJ385672) Dictyostelium discoideum cDNA clone:ddc55c19, 3' ...  1405   0.0    1
(BJ372948) Dictyostelium discoideum cDNA clone:ddc15h02, 3' ...  1402   0.0    1
(BJ354617) Dictyostelium discoideum cDNA clone:dda54p01, 3' ...  1390   0.0    1
(BJ347097) Dictyostelium discoideum cDNA clone:dda26h06, 3' ...  1380   0.0    1
(BJ346962) Dictyostelium discoideum cDNA clone:dda25n15, 3' ...  1374   0.0    1
(BJ401115) Dictyostelium discoideum cDNA clone:dds22n03, 3' ...  1372   0.0    1
(BJ347135) Dictyostelium discoideum cDNA clone:dda26p03, 3' ...  1372   0.0    1
(BJ346761) Dictyostelium discoideum cDNA clone:dda24o20, 3' ...  1370   0.0    1
dna update 2009. 4.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;       431   e-119
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...   428   e-118
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...   183   1e-44
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...   107   7e-22
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    89   4e-16
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    87   1e-15
AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...    82   3e-14
AF245486_1(AF245486|pid:none) Polysphondylium pallidum spore coa...    75   5e-12
AC117076_50(AC117076|pid:none) Dictyostelium discoideum chromoso...    68   6e-10
(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...    64   1e-08
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM520 is

5' end seq. ID AFM520F
5' end seq. 
>AFM520F.Seq
ATTCATTCAATTTTGTAATTATAAAAAAAGGAATCAAATAATTATATATATATATAAAAA
TATAAAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTATTTTTAATTACATTA
TATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAAAGTCATCGTTCAGAA
TATACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTATCATGTTGTGGTAAG
AAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGATGATTTATCATGTGCT
AAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGTAAACCACATAGAGAT
TTTGTACCATATCATTCACCAAATACAACCACATGTGAATCATTAGGTTGTGAAGCAAGA
GGTATGGAATGTGAATGGGTTGAATCATCACCATGTTATGGAACTTCTTGTTGCCCAAGA
ATTCCAAGATGTGTTGGTCATCATGGTGGCGGTCATAAATGTGACAGAATGAGATGTCCA
GAAGGATTCTATTGTGAAGAACAAGGTGGTAGTGCATGCTGCGTACCACATCATGACGGT
TGXXXXXXXXXX
Length of 5' end seq. 612
3' end seq. ID AFM520Z
3' end seq. 
>AFM520Z.Seq
XXXXXXXXXXATTAGCACCAATATCGTGAAATGTTCACCAAAGAATGACGAATGTAAATG
GCATCGTTGTCCACCAGGTTCCAGCTGCTTCAATAGTAGAAACGGTCCACACTGTCTTGC
CAATAATGTATTCCCACAACTTTGTAAAGTTACTCAATGTCCAACTGATTTCTCTTGTAA
AATGATTAGAGGTAATCCAACTTGTATTAAAGCAAGACCACCGGTACCACCACCACATTG
CTCAACTTGTGCAGAGCTATCATCAGCATGTAATCACGTTGGAATGATTTGTATTCAAGT
ACCAAGCAATTGTACCAATACTAGATTCCCATGTTGCCCATCTCATCCAATTTGTATTCA
TCCATCAACTACTGCTGCTTCAACCATTGCAACAACTGCATCAACTGTCGCAACTACAAC
CTCTGCAACTACTGCAGGTACAACAACTGGTGGAACTACAACTGGTGGTTCAACTTCTGA
TAGTAGTGCTGCTTCCTCAGCCGATAGTAGCGCTGCCTCCTCATCACCATCAAGCAGTGC
TGCTTCAAGTGCCGCTTCAAGTGAACCATCAAGTAGCGCCGCTTCAAGTAGTGCTCCATC
CTCTGCTTCAAGTAGCGCTCCATCCTCTGCCCCAAGTAGTGCTCCATCATCATCAGCTTC
AAGTTCAGCTGCATCATCAGCTGCAAGNCTCAGAAAGNCTCAGAAAGCTCATCAGCCACT
TCATAAATAAA
Length of 3' end seq. 731
Connected seq. ID AFM520P
Connected seq. 
>AFM520P.Seq
ATTCATTCAATTTTGTAATTATAAAAAAAGGAATCAAATAATTATATATATATATAAAAA
TATAAAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTATTTTTAATTACATTA
TATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAAAGTCATCGTTCAGAA
TATACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTATCATGTTGTGGTAAG
AAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGATGATTTATCATGTGCT
AAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGTAAACCACATAGAGAT
TTTGTACCATATCATTCACCAAATACAACCACATGTGAATCATTAGGTTGTGAAGCAAGA
GGTATGGAATGTGAATGGGTTGAATCATCACCATGTTATGGAACTTCTTGTTGCCCAAGA
ATTCCAAGATGTGTTGGTCATCATGGTGGCGGTCATAAATGTGACAGAATGAGATGTCCA
GAAGGATTCTATTGTGAAGAACAAGGTGGTAGTGCATGCTGCGTACCACATCATGACGGT
TG----------ATTAGCACCAATATCGTGAAATGTTCACCAAAGAATGACGAATGTAAA
TGGCATCGTTGTCCACCAGGTTCCAGCTGCTTCAATAGTAGAAACGGTCCACACTGTCTT
GCCAATAATGTATTCCCACAACTTTGTAAAGTTACTCAATGTCCAACTGATTTCTCTTGT
AAAATGATTAGAGGTAATCCAACTTGTATTAAAGCAAGACCACCGGTACCACCACCACAT
TGCTCAACTTGTGCAGAGCTATCATCAGCATGTAATCACGTTGGAATGATTTGTATTCAA
GTACCAAGCAATTGTACCAATACTAGATTCCCATGTTGCCCATCTCATCCAATTTGTATT
CATCCATCAACTACTGCTGCTTCAACCATTGCAACAACTGCATCAACTGTCGCAACTACA
ACCTCTGCAACTACTGCAGGTACAACAACTGGTGGAACTACAACTGGTGGTTCAACTTCT
GATAGTAGTGCTGCTTCCTCAGCCGATAGTAGCGCTGCCTCCTCATCACCATCAAGCAGT
GCTGCTTCAAGTGCCGCTTCAAGTGAACCATCAAGTAGCGCCGCTTCAAGTAGTGCTCCA
TCCTCTGCTTCAAGTAGCGCTCCATCCTCTGCCCCAAGTAGTGCTCCATCATCATCAGCT
TCAAGTTCAGCTGCATCATCAGCTGCAAGNCTCAGAAAGNCTCAGAAAGCTCATCAGCCA
CTTCATAAATAAA
Length of connected seq. 1323
Full length Seq ID -
Full length Seq. -
Length of full length seq. -