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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFG201
Library VF
(Link to library)
Clone ID VFG201
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID VFG201P
(Link to Original site)
Representative DNA sequence 
>VFG201 (VFG201Q) /CSM/VF/VFG2-A/VFG201Q.Seq.d/
GTTAAATTTATTTCACAAAAAGTATAATACAAGATGGGTAAAGAAAAAACACATATTAAT
ATCGTCGTTATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTAC
AAATGTGGTGGTATCGATAAAAGAGTTATCGAAAAATACGAAAAGGAAGCTTCTGAAATG
GGTAAAXXXXXXXXXXTGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTTAAA
TCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTC
AACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAA
AACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCA
GGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGT
AAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAA
GGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCTGCTATGGTCGAATTAACCCCATCT
CGCCCAATGTGTGTTGAATCATTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGA
GATATGAGACAAACCGTTGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGT
AAAGCAGGTGATAAGAAAGGTGCTGCTGCTCCATCAAAGAAGAAATAAATAATTTAAAAA
AAATTTTATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
vkfisqkv*YKMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEM
GK---

---VVTFAPAGLSTEVKSVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDP
PQETEKFVAQVIVLNHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTA
AVVLKNGDAAMVELTPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAG
DKKGAAAPSKKK*ii*kkfy
Translated Amino Acid sequence (All Frames) 
Frame A:
vkfisqkv*YKMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEM
GK---

---wssplpqlvsqlklnqskcitnnsqkpvqvtm*vstlktfqskklkevwslvtpkti
hhkklknsllklssstiqvksmlvthqs*ivtlltlpvnslkssiklivvlvpslpkkvl
ppss*rmvmllwsn*phlaqcvlnhslnthh*vvspsei*dkplpsvssnqpsrkpqvkq
virkvlllhqrrnk*fkknfi

Frame B:
lnlfhkkyntrwvkkkhilissllvt*mlvnqpplvi*stnvvvsikelskntkrkllkw
v---

---grhlcpswsln*s*isrnasrttprspsr*qcrfqr*krfsqrn*krygrw*lqkrs
ttrn*kircssyrpqpsrsnpcwlltslrlshcshcl*ih*nrr*s*ssywcrrcqrryc
rrrlkew*ccygrinpispnvc*iih*iptirsfrrqryetnrcrrchqinrqespr*sr
**ercccsikeeinnlkkil

Frame C:
*iyftksiiqdg*rknty*yrrywsrrcw*inhhwsfnlqmwwyr*ksyrkirkgsf*ng
*---

---VVTFAPAGLSTEVKSVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDP
PQETEKFVAQVIVLNHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTA
AVVLKNGDAAMVELTPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAG
DKKGAAAPSKKK*ii*kkfy
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFG201 (VFG201Q) /CSM/VF/VFG2-A/VFG201Q.Seq.d/                       1477   0.0
VFF334 (VFF334Q) /CSM/VF/VFF3-B/VFF334Q.Seq.d/                       1140   0.0
VFE185 (VFE185Q) /CSM/VF/VFE1-D/VFE185Q.Seq.d/                       1140   0.0
SLA633 (SLA633Q) /CSM/SL/SLA6-B/SLA633Q.Seq.d/                       1140   0.0
SFG782 (SFG782Q) /CSM/SF/SFG7-D/SFG782Q.Seq.d/                       1140   0.0
SFC243 (SFC243Q) /CSM/SF/SFC2-B/SFC243Q.Seq.d/                       1140   0.0
FC-BO15 (FC-BO15Q) /CSM/FC/FC-BO/FC-BO15Q.Seq.d/                     1140   0.0
FC-BG17 (FC-BG17Q) /CSM/FC/FC-BG/FC-BG17Q.Seq.d/                     1140   0.0
CHE287 (CHE287Q) /CSM/CH/CHE2-D/CHE287Q.Seq.d/                       1140   0.0
CHB566 (CHB566Q) /CSM/CH/CHB5-C/CHB566Q.Seq.d/                       1140   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.  1132  0.0  2
X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.  1053  0.0  2
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.   896  0.0  2
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   125  e-106  8
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.   125  e-104  8
X00779|X00779.1 Yeast gene for elongation factor 1-alpha (EF-1-alpha).    96  6e-68  6
M10992|M10992.1 Yeast (S.cerevisiae) EF-1-alpha-* (elongation factor 1-alpha) gene (TEF1), complete cds.    96  2e-67  6
AX109679|AX109679.1 Sequence 412 from Patent WO0123604.          101  2e-67  5
M15666|M15666.1 S.cerevisiae EF1 alpha-A gene encoding polypeptide chain elongation factor 1-alpha, complete cds.    96  4e-67  6
AX111255|AX111255.1 Sequence 1988 from Patent WO0123604.          96  6e-67  5
dna update 2003. 9.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   344   2e-93
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   344   2e-93
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   344   2e-93
AY542532_1(AY542532|pid:none) Cladonema radiatum translation elo...   268   1e-70
(Q00251) RecName: Full=Elongation factor 1-alpha;          Short...   268   2e-70
FN392319_1522(FN392319|pid:none) Pichia pastoris GS115 chromosom...   266   4e-70
Y09023_1(Y09023|pid:none) G.cydonium mRNA for elongation factor ...   266   6e-70
(P41745) RecName: Full=Elongation factor 1-alpha;          Short...   266   7e-70
AY643816_1(AY643816|pid:none) Lycogala epidendrum elongation fac...   264   2e-69
AY643820_1(AY643820|pid:none) Arcyria denudata elongation factor...   263   4e-69
protein update 2009. 6.20
PSORT 

 psg: 0.84  gvh: 0.34  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFG201 is nuc
5' end seq. ID VFG201F
5' end seq. 
>VFG201F.Seq
GTTAAATTTATTTCACAAAAAGTATAATACAAGATGGGTAAAGAAAAAACACATATTAAT
ATCGTCGTTATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTAC
AAATGTGGTGGTATCGATAAAAGAGTTATCGAAAAATACGAAAAGGAAGCTTCTGAAATG
GGTAAA----------
Length of 5' end seq. 186
3' end seq. ID VFG201Z
3' end seq. 
>VFG201Z.Seq
----------TGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTTAAATCAGTC
GAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTT
AAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGAT
CCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAA
ATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTC
ACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACT
GCCGCCGTCGTCTTAAAGAATGGTGATGCTGCTATGGTCGAATTAACCCCATCTCGCCCA
ATGTGTGTTGAATCATTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATG
AGACAAACCGTTGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCA
GGTGATAAGAAAGGTGCTGCTGCTCCATCAAAGAAGAAATAAATAATTTAAAAAAAATTT
TATA
Length of 3' end seq. 594
Connected seq. ID VFG201P
Connected seq. 
>VFG201P.Seq
GTTAAATTTATTTCACAAAAAGTATAATACAAGATGGGTAAAGAAAAAACACATATTAAT
ATCGTCGTTATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTAC
AAATGTGGTGGTATCGATAAAAGAGTTATCGAAAAATACGAAAAGGAAGCTTCTGAAATG
GGTAAA----------TGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTTAAA
TCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTC
AACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAA
AACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCA
GGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGT
AAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAA
GGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCTGCTATGGTCGAATTAACCCCATCT
CGCCCAATGTGTGTTGAATCATTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGA
GATATGAGACAAACCGTTGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGT
AAAGCAGGTGATAAGAAAGGTGCTGCTGCTCCATCAAAGAAGAAATAAATAATTTAAAAA
AAATTTTATA
Length of connected seq. 780
Full length Seq ID -
Full length Seq. -
Length of full length seq. -