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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFG184
Library VF
(Link to library)
Clone ID VFG184
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16151-1
Original site URL
Representative seq. ID VFG184P
(Link to Original site)
Representative DNA sequence 
>VFG184 (VFG184Q) /CSM/VF/VFG1-D/VFG184Q.Seq.d/
AATATTTTAATCCCAACTTTTATTATTTATTTATAAGAATGAGACTTCTTATTTCATGTA
TTTTAATACTTTCAATTTTGGTAAACTTTATCAGTGGTCATGCTGTTTTAGTTGCACCAA
CCCCATTTTCTACCAACCCAAGTAAAACTAAACTATGTGGTGGTGGTACAAAACAAACTG
TTGCACAAATTACTTGGTGTCCAAACTCAAGCAAAACAAACAGAGCCACATGGAAAATTG
TTGTTGGTGATGGTGCAGGTGCAGTCACATTTAAATTAGCAACTAATGGTGGAACAACAG
AAGGTGATTTCACTACCACATTAACAAGTAAAGTTCTTTCAGGTAGTGATCCAAAGGAAG
TTGGAACTTATTATATGGATGTCACAGTTCCAACTGGTACCACATGCACAGGTACCAATG
GTATTTGTACACTCCAAGCCTACACAGAAAGCAGTGGTTGGTATTCATGCTCAGCTATTA
AACTTGACTCAAGTGCTTGTXXXXXXXXXXCCCNACNTTATTAAAAANCNCTANTTTANC
NTTGNTTTTAANGNTTTAANAAAAAAATTTCNAAAAAAAAAAAACAAAACAAAAAATCAT
TAATAAAATCACAAAC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
YFNPNFYYLFIRMRLLISCILILSILVNFISGHAVLVAPTPFSTNPSKTKLCGGGTKQTV
AQITWCPNSSKTNRATWKIVVGDGAGAVTFKLATNGGTTEGDFTTTLTSKVLSGSDPKEV
GTYYMDVTVPTGTTCTGTNGICTLQAYTESSGWYSCSAIKLDSSAC---

---PXXIKXXXFXXXFXXLXKKFXKKKNKTKNH**nhk
Translated Amino Acid sequence (All Frames) 
Frame A:
niliptfiiyl*e*dflfhvf*yfqfw*tlsvvmlf*lhqphflptqvklnyvvvvqnkl
lhkllgvqtqakqtephgklllvmvqvqshln*qlmveqqkvislph*qvkffqvviqrk
leliiwmsqfqlvphaqvpmvfvhskptqkavvgihaqllnltqvl---

---PXXIKXXXFXXXFXXLXKKFXKKKNKTKNH**nhk

Frame B:
if*sqlllfiyknetsyfmyfntfnfgklyqwsccfsctnpifyqpk*n*tmwwwyktnc
ctnylvsklkqnkqshmenccw*wcrcshi*isn*wwnnrr*fhyhink*ssfr**skgs
wnllygchssnwyhmhryqwylytpslhrkqwlvfmlsy*t*lkcl---

---ptllkxxxlxlxlxx*xknfxkkktkqkiinkitn

Frame C:
YFNPNFYYLFIRMRLLISCILILSILVNFISGHAVLVAPTPFSTNPSKTKLCGGGTKQTV
AQITWCPNSSKTNRATWKIVVGDGAGAVTFKLATNGGTTEGDFTTTLTSKVLSGSDPKEV
GTYYMDVTVPTGTTCTGTNGICTLQAYTESSGWYSCSAIKLDSSAC---

---xxy*kxlxxxxf*xfxkkiskkkkqnkksliksq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHJ339 (VHJ339Q) /CSM/VH/VHJ3-B/VHJ339Q.Seq.d/                        991   0.0
VFG184 (VFG184Q) /CSM/VF/VFG1-D/VFG184Q.Seq.d/                        991   0.0
VFD405 (VFD405Q) /CSM/VF/VFD4-A/VFD405Q.Seq.d/                        991   0.0
VHG376 (VHG376Q) /CSM/VH/VHG3-D/VHG376Q.Seq.d/                        985   0.0
VFH641 (VFH641Q) /CSM/VF/VFH6-B/VFH641Q.Seq.d/                        977   0.0
VSK140 (VSK140Q) /CSM/VS/VSK1-B/VSK140Q.Seq.d/                        965   0.0
FC-AW02 (FC-AW02Q) /CSM/FC/FC-AW/FC-AW02Q.Seq.d/                      908   0.0
FC-245 (FC-245Q) /CSM/CG/CG-FC/FC-245Q.Seq.d/                         908   0.0
FC-AN19 (FC-AN19Q) /CSM/FC/FC-AN/FC-AN19Q.Seq.d/                      904   0.0
VFC290 (VFC290Q) /CSM/VF/VFC2-D/VFC290Q.Seq.d/                        775   0.0
own update 2002.12.17
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AP003717|AP003717.3 Homo sapiens genomic DNA, chromosome 11q clone:RP11-167N4, complete sequences.    44  0.005  3
AC098647|AC098647.2 Homo sapiens chromosome 3 clone RP11-130G7, complete sequence.    42  0.006  3
AC099779|AC099779.2 Homo sapiens chromosome 3 clone RP11-760G13, complete sequence.    42  0.008  3
AC023597|AC023597.15 Homo sapiens chromosome 3 clone RP11-136H24, WORKING DRAFT SEQUENCE, 8 unordered pieces.    42  0.028  3
AT002541|AT002541.1 Antheraea yamamai posterior silkgland EST, clone AY847.    30  0.053  3
BH255918|BH255918.1 LXL1BAM0003B11f Zea mays L. XL1 methyl filtration maize leaf genomic shotgun library Zea mays genomic clone LXL1BAM0003B11f, DNA sequence.    48  0.13  1
BX005404|BX005404.5 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    42  0.45  2
AC111634|AC111634.4 Rattus norvegicus clone CH230-97M21, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    46  0.51  1
AL023883|AL023883.6 Human DNA sequence from clone RP3-404K8 on chromosome 6p22.2-22.3 Contains PRL (prolactin) gene, STSs and GSSs.    46  0.51  1
AC112833|AC112833.4 Rattus norvegicus clone CH230-335B4, WORKING DRAFT SEQUENCE, 1 ordered piece.    46  0.51  1
dna update 2003. 9.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54UI0) RecName: Full=Uncharacterized transmembrane protein DDB...   213   4e-54
protein update 2009. 2.13
PSORT 

 psg: 0.83  gvh: 0.70  alm: 0.19  top: 0.37  tms: 0.07  mit: 0.47  mip: 0.13
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 1.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 24.0 %: Golgi
	 16.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VFG184 is mit
5' end seq. ID VFG184F
5' end seq. 
>VFG184F.Seq
AATATTTTAATCCCAACTTTTATTATTTATTTATAAGAATGAGACTTCTTATTTCATGTA
TTTTAATACTTTCAATTTTGGTAAACTTTATCAGTGGTCATGCTGTTTTAGTTGCACCAA
CCCCATTTTCTACCAACCCAAGTAAAACTAAACTATGTGGTGGTGGTACAAAACAAACTG
TTGCACAAATTACTTGGTGTCCAAACTCAAGCAAAACAAACAGAGCCACATGGAAAATTG
TTGTTGGTGATGGTGCAGGTGCAGTCACATTTAAATTAGCAACTAATGGTGGAACAACAG
AAGGTGATTTCACTACCACATTAACAAGTAAAGTTCTTTCAGGTAGTGATCCAAAGGAAG
TTGGAACTTATTATATGGATGTCACAGTTCCAACTGGTACCACATGCACAGGTACCAATG
GTATTTGTACACTCCAAGCCTACACAGAAAGCAGTGGTTGGTATTCATGCTCAGCTATTA
AACTTGACTCAAGTGCTTGT----------
Length of 5' end seq. 500
3' end seq. ID VFG184Z
3' end seq. 
>VFG184Z.Seq
----------CCCNACNTTATTAAAAANCNCTANTTTANCNTTGNTTTTAANGNTTTAAN
AAAAAAATTTCNAAAAAAAAAAAACAAAACAAAAAATCATTAATAAAATCACAAAC
Length of 3' end seq. 106
Connected seq. ID VFG184P
Connected seq. 
>VFG184P.Seq
AATATTTTAATCCCAACTTTTATTATTTATTTATAAGAATGAGACTTCTTATTTCATGTA
TTTTAATACTTTCAATTTTGGTAAACTTTATCAGTGGTCATGCTGTTTTAGTTGCACCAA
CCCCATTTTCTACCAACCCAAGTAAAACTAAACTATGTGGTGGTGGTACAAAACAAACTG
TTGCACAAATTACTTGGTGTCCAAACTCAAGCAAAACAAACAGAGCCACATGGAAAATTG
TTGTTGGTGATGGTGCAGGTGCAGTCACATTTAAATTAGCAACTAATGGTGGAACAACAG
AAGGTGATTTCACTACCACATTAACAAGTAAAGTTCTTTCAGGTAGTGATCCAAAGGAAG
TTGGAACTTATTATATGGATGTCACAGTTCCAACTGGTACCACATGCACAGGTACCAATG
GTATTTGTACACTCCAAGCCTACACAGAAAGCAGTGGTTGGTATTCATGCTCAGCTATTA
AACTTGACTCAAGTGCTTGT----------CCCNACNTTATTAAAAANCNCTANTTTANC
NTTGNTTTTAANGNTTTAANAAAAAAATTTCNAAAAAAAAAAAACAAAACAAAAAATCAT
TAATAAAATCACAAAC
Length of connected seq. 606
Full length Seq ID -
Full length Seq. -
Length of full length seq. -