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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFG116
Library VF
(Link to library)
Clone ID VFG116
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFG116P
(Link to Original site)
Representative DNA sequence 
>VFG116 (VFG116Q) /CSM/VF/VFG1-A/VFG116Q.Seq.d/
ATAGTTTTAATAAACAAATATTTAAATATAATACAACAATAAAATGGACGGTGAAGATGT
TCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGA
TGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGN
CGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTT
AACCCTGAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGATATGGAAAAAAT
CTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTNCAGAAGAACACCCAGTTXXXXX
XXXXXGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAAAAGCCCTCTTCCAACCAT
CATTTTTAGGTATGGAATCAGCTGGTATCCNCGAAACCACCTATAACTCCATCATGAAAT
GTGATGTTGANATCCGTAAAGATTTATACGGTAATGTCGTNTTATCTGGTGGNACAACTA
TGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCA
TGAAAATTAAAATCATTGCTCCACCANAACGTAAATACTCTGTCTGGATTGGTGGATCTA
TCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATATGACGAAT
CAGGTCCATCAATTGTCCNCANAAAATGTTTNTAAACAATTAAAACTAGTGATGAAAGTG
CTTCTCACACAAACAAATATATTTAAATGTATAATAATAATAAAATATAATTATTTTTAA
TGGTTGTTNATCTTTATCCGACTATCAAAAAAAAAAAAATATATAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
*f**tni*i*YNNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMX
GMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAXEEHPV--
-

---VITIGNERFRCPKALFQPSFLGMESAGIXETTYNSIMKCDVXIRKDLYGNVVLSGGT
TMFPGIADRMNKELTALAPSTMKIKIIAPPXRKYSVWIGGSILASLSTFQQMWISKEEYD
ESGPSIVXXKCX*tiktsdesashtnkyi*mynnnki*lflmvvxlyptikkkkiy
Translated Amino Acid sequence (All Frames) 
Frame A:
ivlinkylniiqq*ngr*rcssfsyr*wfwyv*srfcw**cptccfpincwsskthwcyg
rygskrllrr**spikerylnpeipn*trycyqlg*ygknlasyflq*tpccxrrtps--
-

---VITIGNERFRCPKALFQPSFLGMESAGIXETTYNSIMKCDVXIRKDLYGNVVLSGGT
TMFPGIADRMNKELTALAPSTMKIKIIAPPXRKYSVWIGGSILASLSTFQQMWISKEEYD
ESGPSIVXXKCX*tiktsdesashtnkyi*mynnnki*lflmvvxlyptikkkkiy

Frame B:
*f**tni*i*YNNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMX
GMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAXEEHPV--
-

---sspsvmnvsvaqkpssnhhf*vwnqlvsxkppitps*nvmlxsvkiytvmsxylvxq
lcsqvlliv*tkn*ll*phqp*klksllhxnvntlsglvdls*lhsqlsnkcgspkknmt
nqvhqlsxxnvxkqlklvmkvlltqtnifkciiiikynyf*wlxifirlskkkkyi

Frame C:
sfnkqifkynttikwtvkmfkl*lsimvlvcvkpvllvmmphvlfshqllvvqdtlvlwx
vwvkktlt*vmkpnqrevs*p*ntqlntvllptgmiwkksgiilstmnsvllqkntq---

---hhhr**tfplpksplptiifrygiswyprnhl*lhhem*c*xp*rfir*crxiwwxn
yvpryc*syeqrincfspinhen*nhcstxt*ilcldwwiylsftlnfptnvdlqrri*r
irsincpxkmfxnn*n***kcfshkqiylnv****niiifngcxslsdyqkkkniy
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFG116 (VFG116Q) /CSM/VF/VFG1-A/VFG116Q.Seq.d/                       1455   0.0
VFO858 (VFO858Q) /CSM/VF/VFO8-C/VFO858Q.Seq.d/                        787   0.0
VFO483 (VFO483Q) /CSM/VF/VFO4-D/VFO483Q.Seq.d/                        787   0.0
VFN701 (VFN701Q) /CSM/VF/VFN7-A/VFN701Q.Seq.d/                        787   0.0
VFM863 (VFM863Q) /CSM/VF/VFM8-C/VFM863Q.Seq.d/                        787   0.0
VFM725 (VFM725Q) /CSM/VF/VFM7-B/VFM725Q.Seq.d/                        787   0.0
VFM720 (VFM720Q) /CSM/VF/VFM7-A/VFM720Q.Seq.d/                        787   0.0
VFM705 (VFM705Q) /CSM/VF/VFM7-A/VFM705Q.Seq.d/                        787   0.0
VFM283 (VFM283Q) /CSM/VF/VFM2-D/VFM283Q.Seq.d/                        787   0.0
VFL589 (VFL589Q) /CSM/VF/VFL5-D/VFL589Q.Seq.d/                        787   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   787  0.0  4
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   686  0.0  4
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.      662  0.0  2
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        611  0.0  4
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   611  0.0  5
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   561  0.0  7
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   492  0.0  5
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   484  0.0  5
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     498  0.0  2
X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.   418  0.0  2
dna update 2003. 9.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A61043(A61043)actin CA15 - sea squirt (Styela clava) (fragments)      201   2e-96
(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   246   6e-64
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   246   6e-64
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   246   6e-64
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        246   6e-64
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   244   3e-63
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   243   5e-63
AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   241   2e-62
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   241   3e-62
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          241   3e-62
protein update 2009. 6.20
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFG116 is nuc
5' end seq. ID VFG116F
5' end seq. 
>VFG116F.Seq
ATAGTTTTAATAAACAAATATTTAAATATAATACAACAATAAAATGGACGGTGAAGATGT
TCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGA
TGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGN
CGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTT
AACCCTGAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGATATGGAAAAAAT
CTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTNCAGAAGAACACCCAGTT-----
-----
Length of 5' end seq. 355
3' end seq. ID VFG116Z
3' end seq. 
>VFG116Z.Seq
----------GTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAAAAGCCCTCTTCCA
ACCATCATTTTTAGGTATGGAATCAGCTGGTATCCNCGAAACCACCTATAACTCCATCAT
GAAATGTGATGTTGANATCCGTAAAGATTTATACGGTAATGTCGTNTTATCTGGTGGNAC
AACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATC
AACCATGAAAATTAAAATCATTGCTCCACCANAACGTAAATACTCTGTCTGGATTGGTGG
ATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATATGA
CGAATCAGGTCCATCAATTGTCCNCANAAAATGTTTNTAAACAATTAAAACTAGTGATGA
AAGTGCTTCTCACACAAACAAATATATTTAAATGTATAATAATAATAAAATATAATTATT
TTTAATGGTTGTTNATCTTTATCCGACTATCAAAAAAAAAAAAATATATAT
Length of 3' end seq. 521
Connected seq. ID VFG116P
Connected seq. 
>VFG116P.Seq
ATAGTTTTAATAAACAAATATTTAAATATAATACAACAATAAAATGGACGGTGAAGATGT
TCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGA
TGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGN
CGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTT
AACCCTGAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGATATGGAAAAAAT
CTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTNCAGAAGAACACCCAGTT-----
-----GTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAAAAGCCCTCTTCCAACCAT
CATTTTTAGGTATGGAATCAGCTGGTATCCNCGAAACCACCTATAACTCCATCATGAAAT
GTGATGTTGANATCCGTAAAGATTTATACGGTAATGTCGTNTTATCTGGTGGNACAACTA
TGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCA
TGAAAATTAAAATCATTGCTCCACCANAACGTAAATACTCTGTCTGGATTGGTGGATCTA
TCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATATGACGAAT
CAGGTCCATCAATTGTCCNCANAAAATGTTTNTAAACAATTAAAACTAGTGATGAAAGTG
CTTCTCACACAAACAAATATATTTAAATGTATAATAATAATAAAATATAATTATTTTTAA
TGGTTGTTNATCTTTATCCGACTATCAAAAAAAAAAAAATATATAT
Length of connected seq. 876
Full length Seq ID -
Full length Seq. -
Length of full length seq. -