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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM502
Library AF
(Link to library)
Clone ID AFM502
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM502P
(Link to Original site)
Representative DNA sequence 
>AFM502 (AFM502Q) /CSM/AF/AFM5-A/AFM502Q.Seq.d/
AATTATTTTAAAATGAGATTATTAGTATTTTTAATATTATTAATTTTTGTAAATTTTTCA
TTTGCAAATGTTAGACCAAATGGTAGAAGATTCTCAGAAAGTCAATATAGAACCGCTTTT
ACTGAATGGACATTAAAATTCAATAGACAATATTCATCAAGTGAATTTAGCAATCGTTAT
TCCATCTTCAAATCAAATATGGACTATGTTGATAACTGGAACTCCAAAGGTGATTCTCAA
ACAGTTTTAGGCTTAAACAATTTTGCTGATATTACAAATGAAGAGTATAGAAAAACTTAT
TTGGGTACCAGAGTTAACGCACATTCCTATAATGGTTATGATGGTCGTGAAGTTTTAAAT
GTTGAAGATCTTCAAACTAATCCAAAATCAATTGATTGGAGAACTAAGAATGCTGTAACT
CCAATTAAAGATCAAGGTCAATGTGGTAGTTGTTGGTCTTTCTCTACTACTGGTAGTACT
GAAGGTGCTCATGCATTAAAAACTAAXXXXXXXXXXAATTTAGTTGATTGCTCTGGTCCA
GAAGAAAACTTTGGTTGTGATGGTGGTTTAATGAATAATGCATTTGATTATATCATTAAA
AACAAGGGTATTGACACTGAAAGTAGTTATCCATATACTGCTGAAACTGGTTCAACATGT
TTATTCAATAAATCTGATATTGGTGCAACCATTAAAGGTTATGTTAACATTACTGCCGGT
TCAGAGATTTCATTAGAGAATGGTGCTCAACATGGTCCAGTATCTGTTGCAATTGATGCT
TCCCATAATAGTTTCCAACTCTATACATCTGGTATCTATTATGAGCCAAAATGTTCTCCA
ACTGAATTGGATCATGGTGTTTTAGTTGTTGGTTATGGTGTTCAAGGTAAAGATGATGAA
GGTCCAGTATTAAACAGAAAACAAACCATTGTTATCCACAAGAATGAAGATAATAAAGTT
GAATCCTCTGATGATTCATCTGATAGTGTTAGACCAAAGGCAAATAATTATTGGATCGTT
AAAAATTCATGGGGTACCTCATGGGGTATAAAAGGTTACATTTTAATGTCAAAGGATAGA
AAGAATAATTGTGGTATTGCATCAGTTAGCTCTTATCCACTTGCCTAGATTTATGGTTCC
AAAATGAATAGGAGAATGTTTGGATCTATCTAGTAACCNAAATGTTTTTCTATTAAAAAA
ATAAAAATAAAAATAA
sequence update 2009. 4. 3
Translated Amino Acid sequence 
NYFKMRLLVFLILLIFVNFSFANVRPNGRRFSESQYRTAFTEWTLKFNRQYSSSEFSNRY
SIFKSNMDYVDNWNSKGDSQTVLGLNNFADITNEEYRKTYLGTRVNAHSYNGYDGREVLN
VEDLQTNPKSIDWRTKNAVTPIKDQGQCGSCWSFSTTGSTEGAHALKT---

---NLVDCSGPEENFGCDGGLMNNAFDYIIKNKGIDTESSYPYTAETGSTCLFNKSDIGA
TIKGYVNITAGSEISLENGAQHGPVSVAIDASHNSFQLYTSGIYYEPKCSPTELDHGVLV
VGYGVQGKDDEGPVLNRKQTIVIHKNEDNKVESSDDSSDSVRPKANNYWIVKNSWGTSWG
IKGYILMSKDRKNNCGIASVSSYPLA*iygskmnrrmfgsi**pkcfsikkikiki
Translated Amino Acid sequence (All Frames) 
Frame A:
NYFKMRLLVFLILLIFVNFSFANVRPNGRRFSESQYRTAFTEWTLKFNRQYSSSEFSNRY
SIFKSNMDYVDNWNSKGDSQTVLGLNNFADITNEEYRKTYLGTRVNAHSYNGYDGREVLN
VEDLQTNPKSIDWRTKNAVTPIKDQGQCGSCWSFSTTGSTEGAHALKT---

---NLVDCSGPEENFGCDGGLMNNAFDYIIKNKGIDTESSYPYTAETGSTCLFNKSDIGA
TIKGYVNITAGSEISLENGAQHGPVSVAIDASHNSFQLYTSGIYYEPKCSPTELDHGVLV
VGYGVQGKDDEGPVLNRKQTIVIHKNEDNKVESSDDSSDSVRPKANNYWIVKNSWGTSWG
IKGYILMSKDRKNNCGIASVSSYPLA*iygskmnrrmfgsi**pkcfsikkikiki

Frame B:
iilk*dy*yf*yy*fl*ifhlqmldqmvedsqkvnieplllngh*nsidnihqvnlaivi
pssnqiwtmlitgtpkvilkqf*a*tillilqmksiekliwvpelthipimvmmvvkf*m
lkifkliqnqligelrml*lqlkikvnvvvvglslllvvlkvlmh*kl---

---i*lialvqkktlvvmvv**imhliislktrvltlkvvihillklvqhvysinlilvq
plkvmltllpvqrfh*rmvlnmvqyllqlmlpiivsnsihlvsimsqnvlqlnwimvf*l
lvmvfkvkmmkvqy*tenkpllstrmkiiklnplmihlivldqrqiiigslkihgvphgv
*kvtf*cqrieriivvlhqlalihlprfmvpk*igecldlssnxnvfllkk*k*k*

Frame C:
lf*neiisifniinfckffickc*tkw*kilrksi*nrfy*mdikiq*tifik*i*qslf
hlqikyglc**lelqr*fsnsfrlkqfc*yyk*rv*knlfgyqs*rtfl*wl*ws*sfkc
*rssn*skin*len*eccnsn*rsrsmw*llvflyyw*y*rcscikn*---

---fs*llwsrrklwl*wwfne*ci*lyh*kqgy*h*k*lsiyc*nwfnmfiq*i*ywcn
h*rlc*hycrfrdfirewcstwssiccn*cfp**fptlyiwyll*akmfsn*igswcfsc
wlwcsr*r**rssikqktnhcypqe*r**s*il**fi**c*tkgk*lldr*kfmgylmgy
krlhfnvkg*ke*lwycis*llstcldlwfqne*envwiylvtxmffy*knknkn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM502 (AFM502Q) /CSM/AF/AFM5-A/AFM502Q.Seq.d/                       2177   0.0
AFO753 (AFO753Q) /CSM/AF/AFO7-C/AFO753Q.Seq.d/                       1338   0.0
AFK758 (AFK758Q) /CSM/AF/AFK7-C/AFK758Q.Seq.d/                       1338   0.0
SSJ524 (SSJ524Q) /CSM/SS/SSJ5-A/SSJ524Q.Seq.d/                       1324   0.0
SSF462 (SSF462Q) /CSM/SS/SSF4-C/SSF462Q.Seq.d/                       1324   0.0
AFO852 (AFO852Q) /CSM/AF/AFO8-C/AFO852Q.Seq.d/                       1312   0.0
SFK650 (SFK650Q) /CSM/SF/SFK6-C/SFK650Q.Seq.d/                       1304   0.0
CFK280 (CFK280Q) /CSM/CF/CFK2-D/CFK280Q.Seq.d/                       1302   0.0
AFK761 (AFK761Q) /CSM/AF/AFK7-C/AFK761Q.Seq.d/                       1298   0.0
AFM621 (AFM621Q) /CSM/AF/AFM6-A/AFM621Q.Seq.d/                       1292   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ347067) Dictyostelium discoideum cDNA clone:dda26c01, 3' ...  1338   0.0    1
(BJ346339) Dictyostelium discoideum cDNA clone:dda30j13, 3' ...  1338   0.0    1
(BJ343263) Dictyostelium discoideum cDNA clone:dda22d15, 3' ...  1338   0.0    1
(X03344) Dictyostelium discoideum mRNA for cysteine proteina...  1312   0.0    2
(C92839) Dictyostelium discoideum slug cDNA, clone SSF462.       1312   0.0    1
(C91266) Dictyostelium discoideum slug cDNA, clone SSJ524.       1312   0.0    1
(BJ346328) Dictyostelium discoideum cDNA clone:dda30h14, 3' ...  1308   0.0    2
(BJ376299) Dictyostelium discoideum cDNA clone:ddc21o20, 3' ...  1304   0.0    2
(BJ343294) Dictyostelium discoideum cDNA clone:dda22j15, 3' ...  1298   0.0    1
(BJ347103) Dictyostelium discoideum cDNA clone:dda26i06, 3' ...  1292   0.0    1
dna update 2009. 4.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P04989) RecName: Full=Cysteine proteinase 2;          EC=3.4.22...   712   0.0
(P54640) RecName: Full=Cysteine proteinase 5;          EC=3.4.22...   382   e-104
L36205_1(L36205|pid:none) Dictyostelium discoideum cysteine prot...   379   e-104
L36204_1(L36204|pid:none) Dictyostelium discoideum cysteine prot...   328   2e-88
(Q94504) RecName: Full=Cysteine proteinase 7;          EC=3.4.22...   315   2e-84
(Q94503) RecName: Full=Cysteine proteinase 6;          EC=3.4.22...   302   1e-80
AY220615_1(AY220615|pid:none) Hydra vulgaris cathepsin L precurs...   287   4e-76
DQ280314_1(DQ280314|pid:none) Hymeniacidon perlevis cathepsin L ...   287   6e-76
AY126713_1(AY126713|pid:none) Metapenaeus ensis cathepsin L prec...   283   6e-75
AY126712_1(AY126712|pid:none) Metapenaeus ensis cathepsin L prec...   283   6e-75
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM502 is

5' end seq. ID AFM502F
5' end seq. 
>AFM502F.Seq
AATTATTTTAAAATGAGATTATTAGTATTTTTAATATTATTAATTTTTGTAAATTTTTCA
TTTGCAAATGTTAGACCAAATGGTAGAAGATTCTCAGAAAGTCAATATAGAACCGCTTTT
ACTGAATGGACATTAAAATTCAATAGACAATATTCATCAAGTGAATTTAGCAATCGTTAT
TCCATCTTCAAATCAAATATGGACTATGTTGATAACTGGAACTCCAAAGGTGATTCTCAA
ACAGTTTTAGGCTTAAACAATTTTGCTGATATTACAAATGAAGAGTATAGAAAAACTTAT
TTGGGTACCAGAGTTAACGCACATTCCTATAATGGTTATGATGGTCGTGAAGTTTTAAAT
GTTGAAGATCTTCAAACTAATCCAAAATCAATTGATTGGAGAACTAAGAATGCTGTAACT
CCAATTAAAGATCAAGGTCAATGTGGTAGTTGTTGGTCTTTCTCTACTACTGGTAGTACT
GAAGGTGCTCATGCATTAAAAACTAAXXXXXXXXXX
Length of 5' end seq. 516
3' end seq. ID AFM502Z
3' end seq. 
>AFM502Z.Seq
XXXXXXXXXXAATTTAGTTGATTGCTCTGGTCCAGAAGAAAACTTTGGTTGTGATGGTGG
TTTAATGAATAATGCATTTGATTATATCATTAAAAACAAGGGTATTGACACTGAAAGTAG
TTATCCATATACTGCTGAAACTGGTTCAACATGTTTATTCAATAAATCTGATATTGGTGC
AACCATTAAAGGTTATGTTAACATTACTGCCGGTTCAGAGATTTCATTAGAGAATGGTGC
TCAACATGGTCCAGTATCTGTTGCAATTGATGCTTCCCATAATAGTTTCCAACTCTATAC
ATCTGGTATCTATTATGAGCCAAAATGTTCTCCAACTGAATTGGATCATGGTGTTTTAGT
TGTTGGTTATGGTGTTCAAGGTAAAGATGATGAAGGTCCAGTATTAAACAGAAAACAAAC
CATTGTTATCCACAAGAATGAAGATAATAAAGTTGAATCCTCTGATGATTCATCTGATAG
TGTTAGACCAAAGGCAAATAATTATTGGATCGTTAAAAATTCATGGGGTACCTCATGGGG
TATAAAAGGTTACATTTTAATGTCAAAGGATAGAAAGAATAATTGTGGTATTGCATCAGT
TAGCTCTTATCCACTTGCCTAGATTTATGGTTCCAAAATGAATAGGAGAATGTTTGGATC
TATCTAGTAACCNAAATGTTTTTCTATTAAAAAAATAAAAATAAAAATAA
Length of 3' end seq. 710
Connected seq. ID AFM502P
Connected seq. 
>AFM502P.Seq
AATTATTTTAAAATGAGATTATTAGTATTTTTAATATTATTAATTTTTGTAAATTTTTCA
TTTGCAAATGTTAGACCAAATGGTAGAAGATTCTCAGAAAGTCAATATAGAACCGCTTTT
ACTGAATGGACATTAAAATTCAATAGACAATATTCATCAAGTGAATTTAGCAATCGTTAT
TCCATCTTCAAATCAAATATGGACTATGTTGATAACTGGAACTCCAAAGGTGATTCTCAA
ACAGTTTTAGGCTTAAACAATTTTGCTGATATTACAAATGAAGAGTATAGAAAAACTTAT
TTGGGTACCAGAGTTAACGCACATTCCTATAATGGTTATGATGGTCGTGAAGTTTTAAAT
GTTGAAGATCTTCAAACTAATCCAAAATCAATTGATTGGAGAACTAAGAATGCTGTAACT
CCAATTAAAGATCAAGGTCAATGTGGTAGTTGTTGGTCTTTCTCTACTACTGGTAGTACT
GAAGGTGCTCATGCATTAAAAACTAA----------AATTTAGTTGATTGCTCTGGTCCA
GAAGAAAACTTTGGTTGTGATGGTGGTTTAATGAATAATGCATTTGATTATATCATTAAA
AACAAGGGTATTGACACTGAAAGTAGTTATCCATATACTGCTGAAACTGGTTCAACATGT
TTATTCAATAAATCTGATATTGGTGCAACCATTAAAGGTTATGTTAACATTACTGCCGGT
TCAGAGATTTCATTAGAGAATGGTGCTCAACATGGTCCAGTATCTGTTGCAATTGATGCT
TCCCATAATAGTTTCCAACTCTATACATCTGGTATCTATTATGAGCCAAAATGTTCTCCA
ACTGAATTGGATCATGGTGTTTTAGTTGTTGGTTATGGTGTTCAAGGTAAAGATGATGAA
GGTCCAGTATTAAACAGAAAACAAACCATTGTTATCCACAAGAATGAAGATAATAAAGTT
GAATCCTCTGATGATTCATCTGATAGTGTTAGACCAAAGGCAAATAATTATTGGATCGTT
AAAAATTCATGGGGTACCTCATGGGGTATAAAAGGTTACATTTTAATGTCAAAGGATAGA
AAGAATAATTGTGGTATTGCATCAGTTAGCTCTTATCCACTTGCCTAGATTTATGGTTCC
AAAATGAATAGGAGAATGTTTGGATCTATCTAGTAACCNAAATGTTTTTCTATTAAAAAA
ATAAAAATAAAAATAA
Length of connected seq. 1206
Full length Seq ID -
Full length Seq. -
Length of full length seq. -