>VFF768 (VFF768Q) /CSM/VF/VFF7-C/VFF768Q.Seq.d/
ACAATTTTAACTTATCATCATTCAAAAATGTTGTCTTCAATTTTAAAAAAAATTCAACCA
TCATTATTAGTTAACTCTAGAATTATCACTAGAACTTATGCTACTAAAGAGGTTACAGTT
CGTGATGCAATTAATAGTGCATTGGATGAAGAATTAGCAAGAGATGAAAAAGTTTTTATT
ATGGGTGAAGAAGTAGCTCAATATAATGGTGCATACAAGATCACTAAAGGATTATTCGAT
AAATATGGTGGTGATAGAATTATTGATACTCCAATTACAGAAGCTGGTTTTGCTGGTATT
GGTGTTGGTGCTGCTATGGCAGGTACTAGACCAATCATTGAATTTATGACTTTCAACTTT
GCTATGCAAGCAATTGATCACATCATCAATTCATCTGCTAAAACTCATTATATGTCTGGT
GGTAAAGTTTTCAATCCAATCGTTTGGAGAGGTCCAAATGGTCCACCAACTGCTGTTGGT
GCTCAACACAGTCAATGTTTTGCTGCATGGTATGGTAGTGTTCCAGGTCTTAAAGTTGTT
GCACCATGGAGTGCTGCTGATCATAGAGGTTTATTAAAATCAGCAATTCGTGATGATXXX
XXXXXXXCCNAAACNTTGNCNANGCTGCTAAAAGAGNTACCCNAANAAATAAATNAATTT
AAAAAAAAAAAAGTCTNAATTAATTTTTTCTCACGAAAAAAAAGAAATAANAAAAGAAAA
A

TILTYHHSKMLSSILKKIQPSLLVNSRIITRTYATKEVTVRDAINSALDEELARDEKVFI
MGEEVAQYNGAYKITKGLFDKYGGDRIIDTPITEAGFAGIGVGAAMAGTRPIIEFMTFNF
AMQAIDHIINSSAKTHYMSGGKVFNPIVWRGPNGPPTAVGAQHSQCFAAWYGSVPGLKVV
APWSAADHRGLLKSAIRDD---

---XTLXXLLKEXPXXINXFKKKKVXINFFSRKKRNXKRK

Frame A:
TILTYHHSKMLSSILKKIQPSLLVNSRIITRTYATKEVTVRDAINSALDEELARDEKVFI
MGEEVAQYNGAYKITKGLFDKYGGDRIIDTPITEAGFAGIGVGAAMAGTRPIIEFMTFNF
AMQAIDHIINSSAKTHYMSGGKVFNPIVWRGPNGPPTAVGAQHSQCFAAWYGSVPGLKVV
APWSAADHRGLLKSAIRDD---

---pkxxxxc*kxypxk*xnlkkkksxlifshekkeixkek

Frame B:
qf*liiiqkcclqf*kkfnhhy*ltlelslelmllkrlqfvmqlivhwmkn*qemkkfll
wvkk*lnimvhtrslkdysinmvviellilqlqklvllvlvlvllwqvldqslnl*lstl
lckqlitssihllkliiclvvkfsiqsfgevqmvhqlllvlntvnvllhgmvvfqvlkll
hhgvlliievy*nqqfvm---

---xnxxxaakrxtxxnkxi*kkksln*ffltkkkk*xkk

Frame C:
nfnlssfknvvfnfkknstiiis*l*nyh*nlcy*rgyss*cn**cig*riskr*ksfyy
g*rsssi*wciqdh*riir*iww**ny*ysnyrswfcwywcwccygry*tnh*iydfqlc
yasn*shhqfic*nslyvww*sfqsnrlerskwstnccwcstqsmfccmvw*csrs*scc
tmecc*s*rfikisns**---

---XTLXXLLKEXPXXINXFKKKKVXINFFSRKKRNXKRK

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF768 (VFF768Q) /CSM/VF/VFF7-C/VFF768Q.Seq.d/                       1148   0.0
VFO292 (VFO292Q) /CSM/VF/VFO2-D/VFO292Q.Seq.d/                       1136   0.0
VFN687 (VFN687Q) /CSM/VF/VFN6-D/VFN687Q.Seq.d/                       1136   0.0
VFK807 (VFK807Q) /CSM/VF/VFK8-A/VFK807Q.Seq.d/                       1136   0.0
FC-BN12 (FC-BN12Q) /CSM/FC/FC-BN/FC-BN12Q.Seq.d/                     1136   0.0
FC-537 (FC-537Q) /CSM/CG/CG-FC/FC-537Q.Seq.d/                        1136   0.0
VFK819 (VFK819Q) /CSM/VF/VFK8-A/VFK819Q.Seq.d/                       1126   0.0
CFI872 (CFI872Q) /CSM/CF/CFI8-C/CFI872Q.Seq.d/                       1124   0.0
SFD835 (SFD835Q) /CSM/SF/SFD8-B/SFD835Q.Seq.d/                       1114   0.0
VFM171 (VFM171Q) /CSM/VF/VFM1-C/VFM171Q.Seq.d/                       1112   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC114263|AC114263.2 Dictyostelium discoideum chromosome 2 map 215673-367476 strain AX4, complete sequence.   765   0.0    6
BU881003|BU881003.1 UM57TE08 Populus flower cDNA library Populus balsamifera subsp. trichocarpa cDNA 5 prime, mRNA sequence.    66   4e-21  4
BQ860256|BQ860256.1 QGC15E06.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGC15E06, mRNA sequence.    60   3e-20  4
BQ862298|BQ862298.1 QGC20L13.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGC20L13, mRNA sequence.    60   3e-20  4
BQ862971|BQ862971.1 QGC22J22.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGC22J22, mRNA sequence.    60   3e-20  4
BQ855000|BQ855000.1 QGB24M14.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGB24M14, mRNA sequence.    60   3e-20  4
BI120224|BI120224.1 F012P16Y Populus flower cDNA library Populus balsamifera subsp. trichocarpa cDNA, mRNA sequence.    66   2e-18  2
CB086276|CB086276.1 hj82c12.g1 Hedyotis centranthoides flower - Stage 2 (NYBG) Hedyotis centranthoides cDNA clone hj82c12, mRNA sequence.    56   4e-18  3
BI121766|BI121766.1 F046P35Y Populus flower cDNA library Populus balsamifera subsp. trichocarpa cDNA, mRNA sequence.    64   7e-18  2
AY070728|AY070728.1 Arabidopsis thaliana AT5g50850/K16E14_1 mRNA, complete cds.    58   1e-17  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86HX0) RecName: Full=Pyruvate dehydrogenase E1 component subun...   380   e-104
(Q38799) RecName: Full=Pyruvate dehydrogenase E1 component subun...   257   2e-67
U09137_1(U09137|pid:none) Arabidopsis thaliana pyruvate dehydrog...   257   2e-67
EU962529_1(EU962529|pid:none) Zea mays clone 243474 pyruvate deh...   256   4e-67
AF069910_1(AF069910|pid:none) Zea mays pyruvate dehydrogenase E1...   255   1e-66
BC084292_1(BC084292|pid:none) Xenopus laevis pyruvate dehydrogen...   254   2e-66
JC5089(JC5089) pyruvate dehydrogenase (lipoamide) (EC 1.2.4.1) b...   254   2e-66
AK104686_1(AK104686|pid:none) Oryza sativa Japonica Group cDNA c...   253   3e-66
BC071117_1(BC071117|pid:none) Xenopus laevis pyruvate dehydrogen...   253   3e-66
AK104686_2(AK104686|pid:none) Oryza sativa Japonica Group cDNA c...   253   5e-66

 psg: 0.71  gvh: 0.44  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.44  mip: 0.05
 nuc: 0.27  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 24.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for VFF768 is nuc

>VFF768F.Seq
ACAATTTTAACTTATCATCATTCAAAAATGTTGTCTTCAATTTTAAAAAAAATTCAACCA
TCATTATTAGTTAACTCTAGAATTATCACTAGAACTTATGCTACTAAAGAGGTTACAGTT
CGTGATGCAATTAATAGTGCATTGGATGAAGAATTAGCAAGAGATGAAAAAGTTTTTATT
ATGGGTGAAGAAGTAGCTCAATATAATGGTGCATACAAGATCACTAAAGGATTATTCGAT
AAATATGGTGGTGATAGAATTATTGATACTCCAATTACAGAAGCTGGTTTTGCTGGTATT
GGTGTTGGTGCTGCTATGGCAGGTACTAGACCAATCATTGAATTTATGACTTTCAACTTT
GCTATGCAAGCAATTGATCACATCATCAATTCATCTGCTAAAACTCATTATATGTCTGGT
GGTAAAGTTTTCAATCCAATCGTTTGGAGAGGTCCAAATGGTCCACCAACTGCTGTTGGT
GCTCAACACAGTCAATGTTTTGCTGCATGGTATGGTAGTGTTCCAGGTCTTAAAGTTGTT
GCACCATGGAGTGCTGCTGATCATAGAGGTTTATTAAAATCAGCAATTCGTGATGAT---
-------

>VFF768Z.Seq
----------CCNAAACNTTGNCNANGCTGCTAAAAGAGNTACCCNAANAAATAAATNAA
TTTAAAAAAAAAAAAGTCTNAATTAATTTTTTCTCACGAAAAAAAAGAAATAANAAAAGA
AAAA

>VFF768P.Seq
ACAATTTTAACTTATCATCATTCAAAAATGTTGTCTTCAATTTTAAAAAAAATTCAACCA
TCATTATTAGTTAACTCTAGAATTATCACTAGAACTTATGCTACTAAAGAGGTTACAGTT
CGTGATGCAATTAATAGTGCATTGGATGAAGAATTAGCAAGAGATGAAAAAGTTTTTATT
ATGGGTGAAGAAGTAGCTCAATATAATGGTGCATACAAGATCACTAAAGGATTATTCGAT
AAATATGGTGGTGATAGAATTATTGATACTCCAATTACAGAAGCTGGTTTTGCTGGTATT
GGTGTTGGTGCTGCTATGGCAGGTACTAGACCAATCATTGAATTTATGACTTTCAACTTT
GCTATGCAAGCAATTGATCACATCATCAATTCATCTGCTAAAACTCATTATATGTCTGGT
GGTAAAGTTTTCAATCCAATCGTTTGGAGAGGTCCAAATGGTCCACCAACTGCTGTTGGT
GCTCAACACAGTCAATGTTTTGCTGCATGGTATGGTAGTGTTCCAGGTCTTAAAGTTGTT
GCACCATGGAGTGCTGCTGATCATAGAGGTTTATTAAAATCAGCAATTCGTGATGAT---
-------CCNAAACNTTGNCNANGCTGCTAAAAGAGNTACCCNAANAAATAAATNAATTT
AAAAAAAAAAAAGTCTNAATTAATTTTTTCTCACGAAAAAAAAGAAATAANAAAAGAAAA
A