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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB476
Library AF
(Link to library)
Clone ID AFB476
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16463-1
Original site URL
Representative seq. ID AFB476P
(Link to Original site)
Representative DNA sequence 
>AFB476 (AFB476Q) /CSM/AF/AFB4-D/AFB476Q.Seq.d/
ATATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATATGTCAGCTCCAGTTTTA
ACCACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAATGACCGCAGGTGTTAAT
GGTCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTGCTCATTTCGATCGTGAA
CGTATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCCATGGTTATTTCGAAGTT
ACCTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAAATAAAGTTGGTAAGAGA
ACACCAATCTTCACCCGTTTCTCTACTGTTGGTGGTGAAAAAGGTTCATCCGATTCTGAA
AGAGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAGGTAACTTTGATATGGTC
GGTAATAATACTCCAGTTTTCTTTATTCGTGATCCATCAAAATTCCCAGATTTCATTCAT
ACACAAAAACGTAATCCACAAACAAATTGTAAAGATCCAAATATGTTTTGGGATTTTTTA
GGTCAAACACCAGAATCAACACATCAAGTATCAATTTTATTCTCTGATAGAGGTACACCA
AAATCATATCGTCATATGCATGGTTTCTCATCACATACCTTAAAATTTGTTAATGCTCAA
GGTAAACCATATXXXXXXXXXXTAGGTGTAAACTATCAACAAATCCCAGTCAATTGTCCA
TTCGCCGTAAAGGGTGGTGTTAAAAACTACCAACGTGATGGTTTCATGGCTGTCAATGGT
AATGGTGGTAAAGGTCCAAACTATCAACCAAACTCTTTCGGTGGTCCAGAGCCACATCCA
GAATTTGCTCAACATAAATTTGACGTCTCTGGTTTCGCTGCTCGTCAACCATACAATCAT
CCAAATGACGACTTTGTTCAACCAGGTGATTTATATAGACTCATGTCTGAAGATGCCAAA
TCTCGTTTCGTCTCTAATTTAGTCGGTCACATGTCCGGTGTCACCATCAAAGAAATTCAA
GTTCGTGCCGTTTCAAACTTTTACAAGGCTGACAAAGATTTAGGTGCTCGTTTATGTAAG
GGTCTTGGTATTGATGTTAATGATGTCATCAAATTCGCTGCTAGATCAAACTTGTAAATC
AATATATTAAAAATAATAAAAAAATAAAAAAATAAAAAAAAATAAAAAATTAATCTCAAT
CTTTTTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
iyfiwek*ink*INMSAPVLTTSSGSPIDNNLNSMTAGVNGPILIQDFTLIDKLAHFDRE
RIPERVVHAKGAGAHGYFEVTSSDVPKWCKAKFLNKVGKRTPIFTRFSTVGGEKGSSDSE
RDPRGFAVKFYTEEGNFDMVGNNTPVFFIRDPSKFPDFIHTQKRNPQTNCKDPNMFWDFL
GQTPESTHQVSILFSDRGTPKSYRHMHGFSSHTLKFVNAQGKPY---

---GVNYQQIPVNCPFAVKGGVKNYQRDGFMAVNGNGGKGPNYQPNSFGGPEPHPEFAQH
KFDVSGFAARQPYNHPNDDFVQPGDLYRLMSEDAKSRFVSNLVGHMSGVTIKEIQVRAVS
NFYKADKDLGARLCKGLGIDVNDVIKFAARSNL*inilkiikk*knkkk*kinlnlf
Translated Amino Acid sequence (All Frames) 
Frame A:
iyfiwek*ink*INMSAPVLTTSSGSPIDNNLNSMTAGVNGPILIQDFTLIDKLAHFDRE
RIPERVVHAKGAGAHGYFEVTSSDVPKWCKAKFLNKVGKRTPIFTRFSTVGGEKGSSDSE
RDPRGFAVKFYTEEGNFDMVGNNTPVFFIRDPSKFPDFIHTQKRNPQTNCKDPNMFWDFL
GQTPESTHQVSILFSDRGTPKSYRHMHGFSSHTLKFVNAQGKPY---

---*v*tinksqsivhsp*rvvlkttnvmvswlsmvmvvkvqtinqtlsvvqshiqnlln
inltslvsllvnhtiiqmttlfnqviyidsclkmpnlvssli*svtcpvspskkfkfvpf
qtftrltki*vlvyvrvlvlmlmmssnslldqtcksiy*k**knkkikknkklisiff

Frame B:
yilfgknk*ink*icqlqf*ppvvvhqliti*tq*pqvlmvqf*fkisl*linllisivn
vsqnvlymlkvlvpmvisklpqvmyqngvklns*iklvrehqsspvslllvvkkvhpilk
eiqevslsnfilkkvtliwsviilqfslfvihqnsqisfihknvihkqivkiqicfgif*
vkhqnqhikyqfyslievhqnhivicmvshhip*nllmlkvnh---

---rcklstnpsqlsirrkgwc*klpt*wfhgcqw*ww*rsklstklfrwsratsricst
*i*rlwfrcsstiqssk*rlcstr*fi*thv*rcqisfrl*fsrshvrchhqrnssscrf
kllqg*qrfrcsfm*gswy*c**chqirc*iklvnqyiknnkkikk*kkikn*sqsf

Frame C:
ifylgkink*inkyvsssfnhq*wftn**qfklndrrc*wsnfnsrfhfn**tcsfrs*t
yprtcctc*rcwcpwlfrsylk*ctkmv*s*ilk*sw*entnlhpflycww*krfirf*k
rskrfrcqily*rr*l*ygr**yssflys*sikiprfhsytkt*stnkl*rskyvlgffr
sntrintssinfil**rytkiissyawflitylkic*csr*ti---

---GVNYQQIPVNCPFAVKGGVKNYQRDGFMAVNGNGGKGPNYQPNSFGGPEPHPEFAQH
KFDVSGFAARQPYNHPNDDFVQPGDLYRLMSEDAKSRFVSNLVGHMSGVTIKEIQVRAVS
NFYKADKDLGARLCKGLGIDVNDVIKFAARSNL*inilkiikk*knkkk*kinlnlf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB476 (AFB476Q) /CSM/AF/AFB4-D/AFB476Q.Seq.d/                       2131   0.0
AFD459 (AFD459Q) /CSM/AF/AFD4-C/AFD459Q.Seq.d/                       1233   0.0
CFI885 (CFI885Q) /CSM/CF/CFI8-D/CFI885Q.Seq.d/                       1225   0.0
AFM195 (AFM195Q) /CSM/AF/AFM1-D/AFM195Q.Seq.d/                       1225   0.0
VFF539 (VFF539Q) /CSM/VF/VFF5-B/VFF539Q.Seq.d/                       1223   0.0
VFA796 (VFA796Q) /CSM/VF/VFA7-D/VFA796Q.Seq.d/                       1221   0.0
AFG803 (AFG803Q) /CSM/AF/AFG8-A/AFG803Q.Seq.d/                       1221   0.0
CFI568 (CFI568Q) /CSM/CF/CFI5-C/CFI568Q.Seq.d/                       1219   0.0
AFO181 (AFO181Q) /CSM/AF/AFO1-D/AFO181Q.Seq.d/                       1203   0.0
AFN639 (AFN639Q) /CSM/AF/AFN6-B/AFN639Q.Seq.d/                       1195   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF090443|AF090443.1 Dictyostelium discoideum catalase (cat) mRNA, complete cds.  1247  0.0  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   924  0.0  5
AB064338|AB064338.1 Candida boidinii CTA1 gene for peroxisomal catalase, complete cds.   111  6e-44  5
AP004595|AP004595.1 Oceanobacillus iheyensis genomic DNA, section 3/13.    72  7e-26  5
AJ005939|AJ005939.1 Methanosarcina barkeri kat gene.              68  1e-17  3
AX536932|AX536932.1 Sequence 533 from Patent WO02064766.         101  1e-17  2
AE006036|AE006036.1 Pasteurella multocida PM70 section 3 of 204 of the complete genome.    98  3e-16  1
AB006327|AB006327.1 Candida albicans mRNA for catalase, complete cds.    94  3e-15  2
E65314|E65314.1 Novel catalase, gene of said catalase and composition containing  said  catalase, and process for preparing catalase using the genetic engineering  technology.    68  6e-15  3
BH404083|BH404083.1 AG-ND-173L2.TR ND-TAM Anopheles gambiae genomic clone AG-ND-173L2, DNA sequence.    56  2e-14  3
dna update 2003. 7.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF090443_1(AF090443|pid:none) Dictyostelium discoideum catalase ...   442   e-123
AY378080_1(AY378080|pid:none) Tetrahymena thermophila putative c...   335   2e-90
(O93662) RecName: Full=Catalase;          EC=1.11.1.6;  &AJ00593...   323   5e-87
CP000300_481(CP000300|pid:none) Methanococcoides burtonii DSM 62...   316   1e-84
CQ796333_1(CQ796333|pid:none) Sequence 109 from Patent WO2004027...   312   1e-83
AE017333_3935(AE017333|pid:none) Bacillus licheniformis DSM 13, ...   312   1e-83
(Q9XZD5) RecName: Full=Peroxisomal catalase;          EC=1.11.1....   311   2e-83
(P26901) RecName: Full=Vegetative catalase;          EC=1.11.1.6...   306   7e-82
CP000560_826(CP000560|pid:none) Bacillus amyloliquefaciens FZB42...   305   3e-81
(P90682) RecName: Full=Catalase;          EC=1.11.1.6;  &Y10611_...   303   1e-80
protein update 2009. 3.27
PSORT 

 psg: 0.89  gvh: 0.57  alm: 0.44  top: 0.40  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: extracellular, including cell wall
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for AFB476 is cyt
5' end seq. ID AFB476F
5' end seq. 
>AFB476F.Seq
ATATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATATGTCAGCTCCAGTTTTA
ACCACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAATGACCGCAGGTGTTAAT
GGTCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTGCTCATTTCGATCGTGAA
CGTATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCCATGGTTATTTCGAAGTT
ACCTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAAATAAAGTTGGTAAGAGA
ACACCAATCTTCACCCGTTTCTCTACTGTTGGTGGTGAAAAAGGTTCATCCGATTCTGAA
AGAGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAGGTAACTTTGATATGGTC
GGTAATAATACTCCAGTTTTCTTTATTCGTGATCCATCAAAATTCCCAGATTTCATTCAT
ACACAAAAACGTAATCCACAAACAAATTGTAAAGATCCAAATATGTTTTGGGATTTTTTA
GGTCAAACACCAGAATCAACACATCAAGTATCAATTTTATTCTCTGATAGAGGTACACCA
AAATCATATCGTCATATGCATGGTTTCTCATCACATACCTTAAAATTTGTTAATGCTCAA
GGTAAACCATAT----------
Length of 5' end seq. 672
3' end seq. ID AFB476Z
3' end seq. 
>AFB476Z.Seq
----------TAGGTGTAAACTATCAACAAATCCCAGTCAATTGTCCATTCGCCGTAAAG
GGTGGTGTTAAAAACTACCAACGTGATGGTTTCATGGCTGTCAATGGTAATGGTGGTAAA
GGTCCAAACTATCAACCAAACTCTTTCGGTGGTCCAGAGCCACATCCAGAATTTGCTCAA
CATAAATTTGACGTCTCTGGTTTCGCTGCTCGTCAACCATACAATCATCCAAATGACGAC
TTTGTTCAACCAGGTGATTTATATAGACTCATGTCTGAAGATGCCAAATCTCGTTTCGTC
TCTAATTTAGTCGGTCACATGTCCGGTGTCACCATCAAAGAAATTCAAGTTCGTGCCGTT
TCAAACTTTTACAAGGCTGACAAAGATTTAGGTGCTCGTTTATGTAAGGGTCTTGGTATT
GATGTTAATGATGTCATCAAATTCGCTGCTAGATCAAACTTGTAAATCAATATATTAAAA
ATAATAAAAAAATAAAAAAATAAAAAAAAATAAAAAATTAATCTCAATCTTTTTT
Length of 3' end seq. 525
Connected seq. ID AFB476P
Connected seq. 
>AFB476P.Seq
ATATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATATGTCAGCTCCAGTTTTA
ACCACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAATGACCGCAGGTGTTAAT
GGTCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTGCTCATTTCGATCGTGAA
CGTATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCCATGGTTATTTCGAAGTT
ACCTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAAATAAAGTTGGTAAGAGA
ACACCAATCTTCACCCGTTTCTCTACTGTTGGTGGTGAAAAAGGTTCATCCGATTCTGAA
AGAGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAGGTAACTTTGATATGGTC
GGTAATAATACTCCAGTTTTCTTTATTCGTGATCCATCAAAATTCCCAGATTTCATTCAT
ACACAAAAACGTAATCCACAAACAAATTGTAAAGATCCAAATATGTTTTGGGATTTTTTA
GGTCAAACACCAGAATCAACACATCAAGTATCAATTTTATTCTCTGATAGAGGTACACCA
AAATCATATCGTCATATGCATGGTTTCTCATCACATACCTTAAAATTTGTTAATGCTCAA
GGTAAACCATAT----------TAGGTGTAAACTATCAACAAATCCCAGTCAATTGTCCA
TTCGCCGTAAAGGGTGGTGTTAAAAACTACCAACGTGATGGTTTCATGGCTGTCAATGGT
AATGGTGGTAAAGGTCCAAACTATCAACCAAACTCTTTCGGTGGTCCAGAGCCACATCCA
GAATTTGCTCAACATAAATTTGACGTCTCTGGTTTCGCTGCTCGTCAACCATACAATCAT
CCAAATGACGACTTTGTTCAACCAGGTGATTTATATAGACTCATGTCTGAAGATGCCAAA
TCTCGTTTCGTCTCTAATTTAGTCGGTCACATGTCCGGTGTCACCATCAAAGAAATTCAA
GTTCGTGCCGTTTCAAACTTTTACAAGGCTGACAAAGATTTAGGTGCTCGTTTATGTAAG
GGTCTTGGTATTGATGTTAATGATGTCATCAAATTCGCTGCTAGATCAAACTTGTAAATC
AATATATTAAAAATAATAAAAAAATAAAAAAATAAAAAAAAATAAAAAATTAATCTCAAT
CTTTTTT
Length of connected seq. 1197
Full length Seq ID -
Full length Seq. -
Length of full length seq. -