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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFF663
Library VF
(Link to library)
Clone ID VFF663
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12898-1
Original site URL
Representative seq. ID VFF663P
(Link to Original site)
Representative DNA sequence 
>VFF663 (VFF663Q) /CSM/VF/VFF6-C/VFF663Q.Seq.d/
AATTAACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTAGAGTTTATGTT
GATGGTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGACAAGCAAGAGAA
TTAGGAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAAAAAATAAAGGT
CCACCAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTAAATGGGCAGAT
GAAGTTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATAGTTTAAACTGT
GATTTTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAGATGTTTATGAA
GGCATTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTGTTTCAACAACC
GAGTTGGTTGGTCGTATGTTATTATGCACCAAAGACCATCTTCAAAATGTTTCAGGTGAG
CAAACCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAATCACCATACACA
AGTTTATCACATTTCCTCCCAACCACCAGAAAGATCGTCCAATTCTCAGAAGGTCGTTCA
CCAAAACCAAACGATAAAAXXXXXXXXXXCGTCCAATTCTCANAAGGTCGTTCNCCAAAA
CCAAACGATAAAATCATTTATATGGATGGTGGTTTCGATTTATTCCATGTAGGTCANACT
GAGGCTTTGAAACAAGCAANAGCACTCGGTGACTATCTCATCGTTGGTGTTCATGATGAT
ANAGTAGTTCNCGAACAAAAAGGTAGCAACTTCCCAATTATGAATTTACATGAACGTGNA
CTCTCTGNACTCTCANGTCGTTATGTCGATGAAGTTGTCATTGGTGCTCCATTCAGTGTC
ACCAAANATATGATTGACTCNCTTCACATCAATGTTGTCGTTCATGGTGATGATCAAGTT
GNACTCGGTCCANAAGGTGGTGTANATCCANACAAACTCCCAANANAATTAGGTATTTAC
AAANAAGTTAAACATACTGAAGGTCNCNCTGCAACTGAAATCGTTAAAAGAATCATTGAT
AATAGACTTCAANATGAAGCTCGNAATCGTAAAAAANAAGCNAAAGAAATTAATTTCNTT
GAACNATCNAATTAAAAAAAATTAAAAAATAAAAAATAAAAATAAAACNAATAAAAAAAA
AAATAGAAAAAAAATT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
n*QVFTMSTTTNKKPIRVYVDGCFDLMHFGHANALRQARELGDILVVGVHTDEEIAKNKG
PPVMNEQERYKAVRACKWADEVAEGAPYTLTEEYLDSLNCDFCVHGEDISVGADGKDVYE
GIKKSGKFRFIKRTEGVSTTELVGRMLLCTKDHLQNVSGEQTSPLGGVNPNVLHKQSPYT
SLSHFLPTTRKIVQFSEGRSPKPNDK---

---VQFSXGRSPKPNDKIIYMDGGFDLFHVGXTEALKQAXALGDYLIVGVHDDXVVXEQK
GSNFPIMNLHERXLSXLSXRYVDEVVIGAPFSVTKXMIDSLHINVVVHGDDQVXLGPXGG
VXPXKLPXXLGIYKXVKHTEGXXATEIVKRIIDNRLQXEARNRKKXAKEINFXEXSN*kk
lknkk*k*nx*kkk*kkn
Translated Amino Acid sequence (All Frames) 
Frame A:
n*QVFTMSTTTNKKPIRVYVDGCFDLMHFGHANALRQARELGDILVVGVHTDEEIAKNKG
PPVMNEQERYKAVRACKWADEVAEGAPYTLTEEYLDSLNCDFCVHGEDISVGADGKDVYE
GIKKSGKFRFIKRTEGVSTTELVGRMLLCTKDHLQNVSGEQTSPLGGVNPNVLHKQSPYT
SLSHFLPTTRKIVQFSEGRSPKPNDK---

---rpilxrsfxktkr*nhlygwwfrfipcrsx*gfetsxstr*lshrwcs**xsssrtk
r*qlpnyeft*txtlxtlxslcr*schwcsiqchqxyd*lxshqccrsw**ssxtrsxrw
cxsxqtpxxirylqxs*ty*rsxcn*nr*knh***tsx*ssxs*kxsxrn*fx*tixlkk
ikk*kikikxikkkiekk

Frame B:
inkflq*vlqliksqlefmlmvvli*cildmqmh*dkqen*eif**lvyiqmkklqkikv
hql*mnkkdikqyvhvngqmklqkvhlil*lkniwiv*tvifvymvkifqlvqmvkmfmk
alkrvvnldssnvlkvfqqpswlvvcyyapktifkmfqvskphhwevsiqtfytnnhhtq
vyhissqpperssnsqkvvhqnqtik---

---VQFSXGRSPKPNDKIIYMDGGFDLFHVGXTEALKQAXALGDYLIVGVHDDXVVXEQK
GSNFPIMNLHERXLSXLSXRYVDEVVIGAPFSVTKXMIDSLHINVVVHGDDQVXLGPXGG
VXPXKLPXXLGIYKXVKHTEGXXATEIVKRIIDNRLQXEARNRKKXAKEINFXEXSN*kk
lknkk*k*nx*kkk*kkn

Frame C:
ltsfyneyyn**kan*slc*wlf*fnafwtckciktskrirryfsswctyr*rnckk*rs
tsye*trki*sstcm*mgr*scrrctlyfn*rifg*fkl*flctw*ryfswcrw*rcl*r
h*kew*i*ihqty*rcfnnrvgwsyvimhqrpsskcfr*anltigrcqskrftqtitihk
fitfppnhqkdrpilrrsftktkr*---

---snsxkvvxqnqtiksfiwmvvsiysm*vxlrl*nkqxhsvtisslvfmmix*fxnkk
vatsql*iymnvxslxsxvvmsmklslvlhsvspxi*ltxftsmlsfmvmiklxsvxkvv
xixtnsqxn*vftxklnilkvxlqlkslkesliidfxmklxivkxkxkklislnxxikkn
*kiknknktnkkknrkki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF663 (VFF663Q) /CSM/VF/VFF6-C/VFF663Q.Seq.d/                       2018   0.0
VFN722 (VFN722Q) /CSM/VF/VFN7-A/VFN722Q.Seq.d/                       1227   0.0
VFF530 (VFF530Q) /CSM/VF/VFF5-B/VFF530Q.Seq.d/                       1227   0.0
VFE318 (VFE318Q) /CSM/VF/VFE3-A/VFE318Q.Seq.d/                       1227   0.0
VFI626 (VFI626Q) /CSM/VF/VFI6-B/VFI626Q.Seq.d/                       1219   0.0
VFG581 (VFG581Q) /CSM/VF/VFG5-D/VFG581Q.Seq.d/                       1219   0.0
CFG421 (CFG421Q) /CSM/CF/CFG4-A/CFG421Q.Seq.d/                       1213   0.0
VFG365 (VFG365Q) /CSM/VF/VFG3-C/VFG365Q.Seq.d/                       1211   0.0
SSK263 (SSK263Q) /CSM/SS/SSK2-C/SSK263Q.Seq.d/                       1209   0.0
VFA266 (VFA266Q) /CSM/VF/VFA2-C/VFA266Q.Seq.d/                       1201   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BG140383|BG140383.1 EST480825 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP17G1 5' sequence, mRNA sequence.    92  3e-16  3
BG131085|BG131085.1 EST463977 tomato crown gall Lycopersicon esculentum cDNA clone cTOE2D11 5' sequence, mRNA sequence.    92  5e-16  3
AW217896|AW217896.1 EST296610 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD1C15, mRNA sequence.    92  3e-15  2
AI778006|AI778006.1 EST258885 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES3N17, mRNA sequence.    92  4e-15  2
AW217261|AW217261.1 EST295975 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC89G22, mRNA sequence.    92  4e-15  2
AW219042|AW219042.1 EST301524 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX2H3, mRNA sequence.    92  4e-15  2
BG138134|BG138134.1 EST478576 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP8K18 5' sequence, mRNA sequence.    92  4e-15  2
BG642411|BG642411.1 EST355887 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD11C15 5' end, mRNA sequence.    92  5e-15  2
BG601025|BG601025.1 EST505920 cSTS Solanum tuberosum cDNA clone cSTS30D8 5' sequence, mRNA sequence.    90  2e-14  2
BI935302|BI935302.1 EST555191 tomato flower, anthesis Lycopersicon esculentum cDNA clone cTOD22O12 5' end, mRNA sequence.    92  2e-14  1
dna update 2003. 9.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55BZ4) RecName: Full=Ethanolamine-phosphate cytidylyltransfera...   667   0.0
AE014134_2379(AE014134|pid:none) Drosophila melanogaster chromos...   350   4e-95
BT077764_1(BT077764|pid:none) Lepeophtheirus salmonis clone lsal...   345   2e-93
(Q5EA75) RecName: Full=Ethanolamine-phosphate cytidylyltransfera...   343   9e-93
AK166319_1(AK166319|pid:none) Mus musculus mammary gland RCB-052...   342   2e-92
AL663030_9(AL663030|pid:none) Mouse DNA sequence from clone RP23...   341   3e-92
AY189524_1(AY189524|pid:none) Mus musculus CTP:ethanolaminephosp...   335   2e-90
AM494969_98(AM494969|pid:none) Leishmania braziliensis chromosom...   333   7e-90
BC088018_1(BC088018|pid:none) Xenopus tropicalis phosphate cytid...   333   7e-90
AM502250_130(AM502250|pid:none) Leishmania infantum chromosome 32.    333   1e-89
protein update 2009. 6.19
PSORT 

 psg: 0.52  gvh: 0.53  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.04
 nuc: 0.08  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFF663 is nuc
5' end seq. ID VFF663F
5' end seq. 
>VFF663F.Seq
AATTAACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTAGAGTTTATGTT
GATGGTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGACAAGCAAGAGAA
TTAGGAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAAAAAATAAAGGT
CCACCAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTAAATGGGCAGAT
GAAGTTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATAGTTTAAACTGT
GATTTTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAGATGTTTATGAA
GGCATTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTGTTTCAACAACC
GAGTTGGTTGGTCGTATGTTATTATGCACCAAAGACCATCTTCAAAATGTTTCAGGTGAG
CAAACCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAATCACCATACACA
AGTTTATCACATTTCCTCCCAACCACCAGAAAGATCGTCCAATTCTCAGAAGGTCGTTCA
CCAAAACCAAACGATAAAA----------
Length of 5' end seq. 619
3' end seq. ID VFF663Z
3' end seq. 
>VFF663Z.Seq
----------CGTCCAATTCTCANAAGGTCGTTCNCCAAAACCAAACGATAAAATCATTT
ATATGGATGGTGGTTTCGATTTATTCCATGTAGGTCANACTGAGGCTTTGAAACAAGCAA
NAGCACTCGGTGACTATCTCATCGTTGGTGTTCATGATGATANAGTAGTTCNCGAACAAA
AAGGTAGCAACTTCCCAATTATGAATTTACATGAACGTGNACTCTCTGNACTCTCANGTC
GTTATGTCGATGAAGTTGTCATTGGTGCTCCATTCAGTGTCACCAAANATATGATTGACT
CNCTTCACATCAATGTTGTCGTTCATGGTGATGATCAAGTTGNACTCGGTCCANAAGGTG
GTGTANATCCANACAAACTCCCAANANAATTAGGTATTTACAAANAAGTTAAACATACTG
AAGGTCNCNCTGCAACTGAAATCGTTAAAAGAATCATTGATAATAGACTTCAANATGAAG
CTCGNAATCGTAAAAAANAAGCNAAAGAAATTAATTTCNTTGAACNATCNAATTAAAAAA
AATTAAAAAATAAAAAATAAAAATAAAACNAATAAAAAAAAAAATAGAAAAAAAATT
Length of 3' end seq. 587
Connected seq. ID VFF663P
Connected seq. 
>VFF663P.Seq
AATTAACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTAGAGTTTATGTT
GATGGTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGACAAGCAAGAGAA
TTAGGAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAAAAAATAAAGGT
CCACCAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTAAATGGGCAGAT
GAAGTTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATAGTTTAAACTGT
GATTTTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAGATGTTTATGAA
GGCATTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTGTTTCAACAACC
GAGTTGGTTGGTCGTATGTTATTATGCACCAAAGACCATCTTCAAAATGTTTCAGGTGAG
CAAACCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAATCACCATACACA
AGTTTATCACATTTCCTCCCAACCACCAGAAAGATCGTCCAATTCTCAGAAGGTCGTTCA
CCAAAACCAAACGATAAAA----------CGTCCAATTCTCANAAGGTCGTTCNCCAAAA
CCAAACGATAAAATCATTTATATGGATGGTGGTTTCGATTTATTCCATGTAGGTCANACT
GAGGCTTTGAAACAAGCAANAGCACTCGGTGACTATCTCATCGTTGGTGTTCATGATGAT
ANAGTAGTTCNCGAACAAAAAGGTAGCAACTTCCCAATTATGAATTTACATGAACGTGNA
CTCTCTGNACTCTCANGTCGTTATGTCGATGAAGTTGTCATTGGTGCTCCATTCAGTGTC
ACCAAANATATGATTGACTCNCTTCACATCAATGTTGTCGTTCATGGTGATGATCAAGTT
GNACTCGGTCCANAAGGTGGTGTANATCCANACAAACTCCCAANANAATTAGGTATTTAC
AAANAAGTTAAACATACTGAAGGTCNCNCTGCAACTGAAATCGTTAAAAGAATCATTGAT
AATAGACTTCAANATGAAGCTCGNAATCGTAAAAAANAAGCNAAAGAAATTAATTTCNTT
GAACNATCNAATTAAAAAAAATTAAAAAATAAAAAATAAAAATAAAACNAATAAAAAAAA
AAATAGAAAAAAAATT
Length of connected seq. 1206
Full length Seq ID -
Full length Seq. -
Length of full length seq. -