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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFF578
Library VF
(Link to library)
Clone ID VFF578
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16173-1
Original site URL
Representative seq. ID VFF578P
(Link to Original site)
Representative DNA sequence 
>VFF578 (VFF578Q) /CSM/VF/VFF5-D/VFF578Q.Seq.d/
AGATAGTCTCCACCAAACGATATTTATATACATCCAATATATTTTTATATATTGAATTAA
AATTTTAAAAAAAGAATGTCAAACAAGGTATTAATGATTGATGGTGATCGTATCAGTGGT
AATGAAGTTAGAGCACAAAATGTTTTAGCAGTTACAGCAATTGCAAATATAGTTAAAACA
TCATTTGGACCAATTGGTTTAGATAAAATGTTAATTGATAATATTGGATCAATTGTTGTT
ACCAATGATGGTGCAACCATTTTACAAAAGATTGATATAGAACATCCAGCAGCAAAGATT
TTAGTACAATTATCAGAATTGCAAGATCAAGAAGTTGGTGATGGTACAACTACAGTTGTA
ATTTTAGCAGCAGAATTATTGAAACGTGCCAATGAATTGGTAGCACGTAAAGTACATCCA
ACAGTTATTATTAGTGGTTTTAGATTAGCATGTACAGAAGCAATCAAATATATCAACGAA
ACATTAGCAGTTAAAGTTGAAACCTTACCAAAGGATTTCATTGTAAACATTGCCAAGACA
TCAATGTCATCAAAGACCATCAATGACGATAGTGATTTCTTTTCAAAGATTGTCATTGAG
GCAATCACTCGTGTGAAAACCATXXXXXXXXXXAGATCTTAAACGTATCGCCAAATCATG
TGGTGGTACAGTCTTAATCACACTTGCAAATCTCGAGGGTGAAGAATCATTTGATACCAC
CGCCTTGGGTATTGCCGATGAAGTTGTTCAAGATAGATTGGCCGATGATGAATTAATCAT
TGTCAAAAACTCAAATAAGAAATCAGCTTCAATCATTCTTCGTGGTGCCAATGAATTAAT
GTTGGATGAAATGGAACGTTCCATTCATGACTCTCTCTGTATCGTAAAGAGAACTTTAGA
ATCTGGAACCATTGTTCCAGGTGGTGGTGCAGTTGAATCTGCCCTCTCTATCTATCTCGA
TAATATTGCCGCCACAATGGGTAGTCGTAAACAATTAGCAATCTCTGAATTTGCTGAATC
CCTCTTGGTTGTACCAAAACAATTGGCTGTAAATGCTGCTCTTGATGCCTCTGATTTAGT
TTCAAAATTAAAAGCCTATCATCATGCCGCTCAAACTGATCCATCAAAGAAATCTTACGC
ATACTCTGGTCTCGATTTATTCAATAACAAAGTTAGAAATAATTTAGAGGCTGGTGTTTT
AGAACCTGCCATTGCCAAAATCAAATGTATCAAATTTGCAACTGAAAGCGCTATCACCAT
CCTTCGTANCGAATGTATAAAATCACNCNNAAACCAAGAGTAACAACAAG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
r*sppndiyihpiyfyiln*NFKKRMSNKVLMIDGDRISGNEVRAQNVLAVTAIANIVKT
SFGPIGLDKMLIDNIGSIVVTNDGATILQKIDIEHPAAKILVQLSELQDQEVGDGTTTVV
ILAAELLKRANELVARKVHPTVIISGFRLACTEAIKYINETLAVKVETLPKDFIVNIAKT
SMSSKTINDDSDFFSKIVIEAITRVKT---

---DLKRIAKSCGGTVLITLANLEGEESFDTTALGIADEVVQDRLADDELIIVKNSNKKS
ASIILRGANELMLDEMERSIHDSLCIVKRTLESGTIVPGGGAVESALSIYLDNIAATMGS
RKQLAISEFAESLLVVPKQLAVNAALDASDLVSKLKAYHHAAQTDPSKKSYAYSGLDLFN
NKVRNNLEAGVLEPAIAKIKCIKFATESAITILRXECIKSXXNQE*qq
Translated Amino Acid sequence (All Frames) 
Frame A:
r*sppndiyihpiyfyiln*NFKKRMSNKVLMIDGDRISGNEVRAQNVLAVTAIANIVKT
SFGPIGLDKMLIDNIGSIVVTNDGATILQKIDIEHPAAKILVQLSELQDQEVGDGTTTVV
ILAAELLKRANELVARKVHPTVIISGFRLACTEAIKYINETLAVKVETLPKDFIVNIAKT
SMSSKTINDDSDFFSKIVIEAITRVKT---

---rs*tyrqimwwyslnhtcksrg*rii*yhrlgycr*scsr*igr**inhcqklk*ei
sfnhsswcq*invg*ngtfhs*lslyrkenfriwnhcsrwwcs*icplylsr*ycrhng*
s*tisnl*ic*iplgctktigckccs*cl*fsfkikslsscrsn*sikeilrilwsrfiq
*qs*k*frgwcfrtchcqnqmyqicn*kryhhpsxrmykitxkprvtt

Frame B:
dslhqtifiyiqyifiy*ikilkkecqtry**lmvivsvvmklehkmf*qlqqlqi*lkh
hldqlv*ikc*liildqlllpmmvqpfykrli*niqqqrf*ynyqnckikklvmvqlql*
f*qqny*nvpmnw*hvkyiqqlllvvld*hvqkqsnistkh*qlklkpyqrisl*tlprh
qchqrpsmtivisfqrlslrqslv*kp---

---DLKRIAKSCGGTVLITLANLEGEESFDTTALGIADEVVQDRLADDELIIVKNSNKKS
ASIILRGANELMLDEMERSIHDSLCIVKRTLESGTIVPGGGAVESALSIYLDNIAATMGS
RKQLAISEFAESLLVVPKQLAVNAALDASDLVSKLKAYHHAAQTDPSKKSYAYSGLDLFN
NKVRNNLEAGVLEPAIAKIKCIKFATESAITILRXECIKSXXNQE*qq

Frame C:
ivstkrylytsniflyielkf*kknvkqgind*w*syqw**s*stkcfssysnckys*ni
iwtnwfr*nvn**ywinccyq*wcnhftkd*yrtssskdfstiiriarsrsw*wynyscn
fssriietcq*igst*stsnsyy*wf*ismyrsnqiyqrniss*s*nltkgfhckhcqdi
nvikdhq*r**flfkdch*gnhscenh---

---ilnvspnhvvvqs*shlqisrvknhlipppwvlpmklfkidwpmmn*slsktqirnq
lqsffvvpmn*cwmkwnvpfmtlsvs*rel*nleplfqvvvqlnlpslsisiilppqwvv
vnn*qslnllnpswlyqnnwl*mlllmpli*fqn*kpiimplklihqrnlthtlvsiysi
tkleii*rlvf*nlplpksnvsnlqlkalspsfvxnv*nhxxtksnnk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF578 (VFF578Q) /CSM/VF/VFF5-D/VFF578Q.Seq.d/                       2472   0.0
CFH434 (CFH434Q) /CSM/CF/CFH4-B/CFH434Q.Seq.d/                       1263   0.0
AFF451 (AFF451Q) /CSM/AF/AFF4-C/AFF451Q.Seq.d/                       1263   0.0
AFD439 (AFD439Q) /CSM/AF/AFD4-B/AFD439Q.Seq.d/                       1263   0.0
AFG366 (AFG366Q) /CSM/AF/AFG3-C/AFG366Q.Seq.d/                       1251   0.0
VFD589 (VFD589Q) /CSM/VF/VFD5-D/VFD589Q.Seq.d/                       1243   0.0
AHI886 (AHI886Q) /CSM/AH/AHI8-D/AHI886Q.Seq.d/                       1185   0.0
VFA730 (VFA730Q) /CSM/VF/VFA7-B/VFA730Q.Seq.d/                       1181   0.0
FC-AG03 (FC-AG03Q) /CSM/FC/FC-AG/FC-AG03Q.Seq.d/                     1172   0.0
FC-116 (FC-116Q) /CSM/CG/CG-FC/FC-116Q.Seq.d/                        1172   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AB008158|AB008158.1 Dictyostelium discoideum DdTcp-1 mRNA for t-complex polypeptide 1 homologue, complete cds.  1261  0.0  3
AZ530054|AZ530054.1 ENTDW96TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    66  2e-16  3
AZ526360|AZ526360.1 254PbF04 Pb MBN #21 Plasmodium berghei genomic 3', DNA sequence.    70  2e-14  3
AZ522254|AZ522254.1 204PbE04 Pb MBN #21 Plasmodium berghei genomic 3', DNA sequence.    68  1e-12  2
BF298951|BF298951.1 068PbG10 Pb cDNA #20, Charles Yowell and Jane Carlton Plasmodium berghei cDNA 5', mRNA sequence.    68  1e-12  2
BF299139|BF299139.1 069PbF12 Pb cDNA #20, Charles Yowell and Jane Carlton Plasmodium berghei cDNA 5', mRNA sequence.    68  1e-12  2
AL437502|AL437502.1 T3 end of clone BC0AA009G02 of library BC0AA from strain CBS 767 of Debaryomyces hansenii.    48  3e-10  4
AF143494|AF143494.1 Xenopus laevis t-complex polypeptide 1 (TCP1) mRNA, complete cds.    48  1e-08  5
AF143496|AF143496.1 Paleosuchus palpebrosus t-complex polypeptide 1 (TCP1) mRNA, complete cds.    38  5e-07  5
AX488977|AX488977.1 Sequence 6277 from Patent WO02053728.         42  8e-07  4
dna update 2003. 9.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB008158_1(AB008158|pid:none) Dictyostelium discoideum DdTcp-1 m...   401   e-110
AF442545_1(AF442545|pid:none) Physarum polycephalum CCT chaperon...   308   3e-82
BT002052_1(BT002052|pid:none) Arabidopsis thaliana t-complex pol...   278   2e-73
(P18279) RecName: Full=T-complex protein 1 subunit alpha;       ...   278   3e-73
AY891878_1(AY891878|pid:none) Synthetic construct Homo sapiens c...   277   7e-73
(Q4R5G2) RecName: Full=T-complex protein 1 subunit alpha;       ...   277   7e-73
(P28480) RecName: Full=T-complex protein 1 subunit alpha;       ...   276   2e-72
(Q32L40) RecName: Full=T-complex protein 1 subunit alpha;       ...   275   3e-72
AF143494_1(AF143494|pid:none) Xenopus laevis t-complex polypepti...   275   3e-72
M20130_1(M20130|pid:none) Mouse t complex polypeptide 1 (Tcp-1-a...   273   8e-72
protein update 2009. 6.19
PSORT 

 psg: 0.57  gvh: 0.40  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: mitochondrial
	 28.0 %: nuclear
	  4.0 %: vesicles of secretory system

>> prediction for VFF578 is cyt
5' end seq. ID VFF578F
5' end seq. 
>VFF578F.Seq
AGATAGTCTCCACCAAACGATATTTATATACATCCAATATATTTTTATATATTGAATTAA
AATTTTAAAAAAAGAATGTCAAACAAGGTATTAATGATTGATGGTGATCGTATCAGTGGT
AATGAAGTTAGAGCACAAAATGTTTTAGCAGTTACAGCAATTGCAAATATAGTTAAAACA
TCATTTGGACCAATTGGTTTAGATAAAATGTTAATTGATAATATTGGATCAATTGTTGTT
ACCAATGATGGTGCAACCATTTTACAAAAGATTGATATAGAACATCCAGCAGCAAAGATT
TTAGTACAATTATCAGAATTGCAAGATCAAGAAGTTGGTGATGGTACAACTACAGTTGTA
ATTTTAGCAGCAGAATTATTGAAACGTGCCAATGAATTGGTAGCACGTAAAGTACATCCA
ACAGTTATTATTAGTGGTTTTAGATTAGCATGTACAGAAGCAATCAAATATATCAACGAA
ACATTAGCAGTTAAAGTTGAAACCTTACCAAAGGATTTCATTGTAAACATTGCCAAGACA
TCAATGTCATCAAAGACCATCAATGACGATAGTGATTTCTTTTCAAAGATTGTCATTGAG
GCAATCACTCGTGTGAAAACCAT----------
Length of 5' end seq. 623
3' end seq. ID VFF578Z
3' end seq. 
>VFF578Z.Seq
----------AGATCTTAAACGTATCGCCAAATCATGTGGTGGTACAGTCTTAATCACAC
TTGCAAATCTCGAGGGTGAAGAATCATTTGATACCACCGCCTTGGGTATTGCCGATGAAG
TTGTTCAAGATAGATTGGCCGATGATGAATTAATCATTGTCAAAAACTCAAATAAGAAAT
CAGCTTCAATCATTCTTCGTGGTGCCAATGAATTAATGTTGGATGAAATGGAACGTTCCA
TTCATGACTCTCTCTGTATCGTAAAGAGAACTTTAGAATCTGGAACCATTGTTCCAGGTG
GTGGTGCAGTTGAATCTGCCCTCTCTATCTATCTCGATAATATTGCCGCCACAATGGGTA
GTCGTAAACAATTAGCAATCTCTGAATTTGCTGAATCCCTCTTGGTTGTACCAAAACAAT
TGGCTGTAAATGCTGCTCTTGATGCCTCTGATTTAGTTTCAAAATTAAAAGCCTATCATC
ATGCCGCTCAAACTGATCCATCAAAGAAATCTTACGCATACTCTGGTCTCGATTTATTCA
ATAACAAAGTTAGAAATAATTTAGAGGCTGGTGTTTTAGAACCTGCCATTGCCAAAATCA
AATGTATCAAATTTGCAACTGAAAGCGCTATCACCATCCTTCGTANCGAATGTATAAAAT
CACNCNNAAACCAAGAGTAACAACAAG
Length of 3' end seq. 677
Connected seq. ID VFF578P
Connected seq. 
>VFF578P.Seq
AGATAGTCTCCACCAAACGATATTTATATACATCCAATATATTTTTATATATTGAATTAA
AATTTTAAAAAAAGAATGTCAAACAAGGTATTAATGATTGATGGTGATCGTATCAGTGGT
AATGAAGTTAGAGCACAAAATGTTTTAGCAGTTACAGCAATTGCAAATATAGTTAAAACA
TCATTTGGACCAATTGGTTTAGATAAAATGTTAATTGATAATATTGGATCAATTGTTGTT
ACCAATGATGGTGCAACCATTTTACAAAAGATTGATATAGAACATCCAGCAGCAAAGATT
TTAGTACAATTATCAGAATTGCAAGATCAAGAAGTTGGTGATGGTACAACTACAGTTGTA
ATTTTAGCAGCAGAATTATTGAAACGTGCCAATGAATTGGTAGCACGTAAAGTACATCCA
ACAGTTATTATTAGTGGTTTTAGATTAGCATGTACAGAAGCAATCAAATATATCAACGAA
ACATTAGCAGTTAAAGTTGAAACCTTACCAAAGGATTTCATTGTAAACATTGCCAAGACA
TCAATGTCATCAAAGACCATCAATGACGATAGTGATTTCTTTTCAAAGATTGTCATTGAG
GCAATCACTCGTGTGAAAACCAT----------AGATCTTAAACGTATCGCCAAATCATG
TGGTGGTACAGTCTTAATCACACTTGCAAATCTCGAGGGTGAAGAATCATTTGATACCAC
CGCCTTGGGTATTGCCGATGAAGTTGTTCAAGATAGATTGGCCGATGATGAATTAATCAT
TGTCAAAAACTCAAATAAGAAATCAGCTTCAATCATTCTTCGTGGTGCCAATGAATTAAT
GTTGGATGAAATGGAACGTTCCATTCATGACTCTCTCTGTATCGTAAAGAGAACTTTAGA
ATCTGGAACCATTGTTCCAGGTGGTGGTGCAGTTGAATCTGCCCTCTCTATCTATCTCGA
TAATATTGCCGCCACAATGGGTAGTCGTAAACAATTAGCAATCTCTGAATTTGCTGAATC
CCTCTTGGTTGTACCAAAACAATTGGCTGTAAATGCTGCTCTTGATGCCTCTGATTTAGT
TTCAAAATTAAAAGCCTATCATCATGCCGCTCAAACTGATCCATCAAAGAAATCTTACGC
ATACTCTGGTCTCGATTTATTCAATAACAAAGTTAGAAATAATTTAGAGGCTGGTGTTTT
AGAACCTGCCATTGCCAAAATCAAATGTATCAAATTTGCAACTGAAAGCGCTATCACCAT
CCTTCGTANCGAATGTATAAAATCACNCNNAAACCAAGAGTAACAACAAG
Length of connected seq. 1300
Full length Seq ID -
Full length Seq. -
Length of full length seq. -