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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFF530
Library VF
(Link to library)
Clone ID VFF530
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12898-1
Original site URL
Representative seq. ID VFF530E
(Link to Original site)
Representative DNA sequence 
>VFF530 (VFF530Q) /CSM/VF/VFF5-B/VFF530Q.Seq.d/
TTGGCCTACTGGAATTAACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATT
AGAGTTTATGTTGATGGTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGA
CAAGCAAGAGAATTAGGAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCA
AAAAATAAAGGTCCACCAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGT
AAATGGGCAGATGAAGTTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGAT
AGTTTAAACTGTGATTTTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAA
GATGTTTATGAAGGCATTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGT
GTTTCAACAACCGAGTTGGTTGGTCGTATGTTATTATGCACCAAAGACCATCTTCAAAAT
GTTTCAGGTGAGCAAACCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAA
TCACCATACACAAGTTTATCACATTTCCTCCCAACCACCAGAAAGATCGTCCAATTCTCA
GAAGGTCGTTCACCAAAACCAAACGATAAAATCATTTATATGGATGGTGGTTTCGATTTA
TTCCATGTAGGTCATACTGAGGCTTTGAAACAAGCAAGAGCACTCGGTGACTATCTCATC
GTTGGTGTTCATGATGATAGAGTAGTTCACGAACAAAAAGGTAGCAACTTCCCAATTATG
AATTTACATGAACGTGTACTCTCTGTACTCTCATGTCGTTATGTCGATGAAGTTGTCATT
GGTGCTCCATTCAGTGTCACCAAGGATATGATTGACTCACTTCACATCAATGTTGTCGTT
CATGGTGATGATCAAGTTGTACTCGGTCCAGAAGGTGGNGNANANCCANACAAACTCCCA
AAANAANTAGGT
sequence update 2001. 6. 9
Translated Amino Acid sequence 
laywn*QVFTMSTTTNKKPIRVYVDGCFDLMHFGHANALRQARELGDILVVGVHTDEEIA
KNKGPPVMNEQERYKAVRACKWADEVAEGAPYTLTEEYLDSLNCDFCVHGEDISVGADGK
DVYEGIKKSGKFRFIKRTEGVSTTELVGRMLLCTKDHLQNVSGEQTSPLGGVNPNVLHKQ
SPYTSLSHFLPTTRKIVQFSEGRSPKPNDKIIYMDGGFDLFHVGHTEALKQARALGDYLI
VGVHDDRVVHEQKGSNFPIMNLHERVLSVLSCRYVDEVVIGAPFSVTKDMIDSLHINVVV
HGDDQVVLGPEGGXXPXKLPKXXG
Translated Amino Acid sequence (All Frames) 
Frame A:
laywn*QVFTMSTTTNKKPIRVYVDGCFDLMHFGHANALRQARELGDILVVGVHTDEEIA
KNKGPPVMNEQERYKAVRACKWADEVAEGAPYTLTEEYLDSLNCDFCVHGEDISVGADGK
DVYEGIKKSGKFRFIKRTEGVSTTELVGRMLLCTKDHLQNVSGEQTSPLGGVNPNVLHKQ
SPYTSLSHFLPTTRKIVQFSEGRSPKPNDKIIYMDGGFDLFHVGHTEALKQARALGDYLI
VGVHDDRVVHEQKGSNFPIMNLHERVLSVLSCRYVDEVVIGAPFSVTKDMIDSLHINVVV
HGDDQVVLGPEGGXXPXKLPKXXG


Frame B:
wptginkflq*vlqliksqlefmlmvvli*cildmqmh*dkqen*eif**lvyiqmkklq
kikvhql*mnkkdikqyvhvngqmklqkvhlil*lkniwiv*tvifvymvkifqlvqmvk
mfmkalkrvvnldssnvlkvfqqpswlvvcyyapktifkmfqvskphhwevsiqtfytnn
hhtqvyhissqpperssnsqkvvhqnqtiksfiwmvvsiysm*vilrl*nkqehsvtiss
lvfmmie*ftnkkvatsql*iymnvyslyshvvmsmklslvlhsvspri*lthftsmlsf
mvmiklysvqkvxxxxtnsqxx*


Frame C:
glleltsfyneyyn**kan*slc*wlf*fnafwtckciktskrirryfsswctyr*rnck
k*rstsye*trki*sstcm*mgr*scrrctlyfn*rifg*fkl*flctw*ryfswcrw*r
cl*rh*kew*i*ihqty*rcfnnrvgwsyvimhqrpsskcfr*anltigrcqskrftqti
tihkfitfppnhqkdrpilrrsftktkr*nhlygwwfrfipcrsy*gfetskstr*lshr
wcs***sssrtkr*qlpnyeft*tctlctlmslcr*schwcsiqchqgyd*ltshqccrs
w**ssctrsrrwxxxxqtpkxxr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF530 (VFF530Q) /CSM/VF/VFF5-B/VFF530Q.Seq.d/                       1885   0.0
VFN722 (VFN722Q) /CSM/VF/VFN7-A/VFN722Q.Seq.d/                       1846   0.0
VFI626 (VFI626Q) /CSM/VF/VFI6-B/VFI626Q.Seq.d/                       1838   0.0
CFG421 (CFG421Q) /CSM/CF/CFG4-A/CFG421Q.Seq.d/                       1832   0.0
VFG365 (VFG365Q) /CSM/VF/VFG3-C/VFG365Q.Seq.d/                       1830   0.0
VFA266 (VFA266Q) /CSM/VF/VFA2-C/VFA266Q.Seq.d/                       1820   0.0
VFJ833 (VFJ833Q) /CSM/VF/VFJ8-B/VFJ833Q.Seq.d/                       1812   0.0
VFE318 (VFE318Q) /CSM/VF/VFE3-A/VFE318Q.Seq.d/                       1788   0.0
VFM413 (VFM413Q) /CSM/VF/VFM4-A/VFM413Q.Seq.d/                       1750   0.0
VFG517 (VFG517Q) /CSM/VF/VFG5-A/VFG517Q.Seq.d/                       1742   0.0
own update 2001.12. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BG140383|BG140383.1 EST480825 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP17G1 5' sequence, mRNA sequence.    92  2e-16  3
BG131085|BG131085.1 EST463977 tomato crown gall Lycopersicon esculentum cDNA clone cTOE2D11 5' sequence, mRNA sequence.    92  2e-16  3
AW217896|AW217896.1 EST296610 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD1C15, mRNA sequence.    92  2e-15  2
AI778006|AI778006.1 EST258885 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES3N17, mRNA sequence.    92  2e-15  2
AW217261|AW217261.1 EST295975 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC89G22, mRNA sequence.    92  3e-15  2
AW219042|AW219042.1 EST301524 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX2H3, mRNA sequence.    92  3e-15  2
BG138134|BG138134.1 EST478576 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP8K18 5' sequence, mRNA sequence.    92  3e-15  2
BG642411|BG642411.1 EST355887 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD11C15 5' end, mRNA sequence.    92  3e-15  2
BG601025|BG601025.1 EST505920 cSTS Solanum tuberosum cDNA clone cSTS30D8 5' sequence, mRNA sequence.    90  1e-14  2
BG133537|BG133537.1 EST466525 tomato crown gall Lycopersicon esculentum cDNA clone cTOE13N15 5' sequence, mRNA sequence.    92  2e-14  1
dna update 2003. 9.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55BZ4) RecName: Full=Ethanolamine-phosphate cytidylyltransfera...   631   e-180
AE014134_2379(AE014134|pid:none) Drosophila melanogaster chromos...   352   1e-95
AM502250_130(AM502250|pid:none) Leishmania infantum chromosome 32.    350   3e-95
BT077764_1(BT077764|pid:none) Lepeophtheirus salmonis Pacific fo...   347   3e-94
AM494969_98(AM494969|pid:none) Leishmania braziliensis chromosom...   345   2e-93
CT005269_94(CT005269|pid:none) Leishmania major strain Friedlin,...   340   4e-92
(Q5EA75) RecName: Full=Ethanolamine-phosphate cytidylyltransfera...   338   2e-91
AK166319_1(AK166319|pid:none) Mus musculus mammary gland RCB-052...   337   4e-91
AL663030_9(AL663030|pid:none) Mouse DNA sequence from clone RP23...   337   5e-91
AY189524_1(AY189524|pid:none) Mus musculus CTP:ethanolaminephosp...   330   3e-89
protein update 2009. 7.10
PSORT 

 psg: 0.52  gvh: 0.53  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: mitochondrial
	 36.0 %: cytoplasmic
	 16.0 %: nuclear
	  4.0 %: vesicles of secretory system

>> prediction for VFF530 is mit
5' end seq. ID VFF530F
5' end seq. 
>VFF530F.Seq
TTGGCCTACTGGAATTAACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATT
AGAGTTTATGTTGATGGTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGA
CAAGCAAGAGAATTAGGAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCA
AAAAATAAAGGTCCACCAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGT
AAATGGGCAGATGAAGTTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGAT
AGTTTAAACTGTGATTTTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAA
GATGTTTATGAAGGCATTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGT
GTTTCAACAACCGAGTTGGTTGGTCGTATGTTATTATGCACCAAAGACCATCTTCAAAAT
GTTTCAGGTGAGCAAACCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAA
TCACCATACACAAGTTTATCACATTTCCTCC----------
Length of 5' end seq. 571
3' end seq. ID VFF530Z
3' end seq. 
>VFF530Z.Seq
----------TGTCAATCCAAACGTTTTACACAAACAATCACCATACACAAGTTTATCAC
ATTTCCTCCCAACCACCAGAAAGATCGTCCAATTCTCAGAAGGTCGTTCACCAAAACCAA
ACGATAAAATCATTTATATGGATGGTGGTTTCGATTTATTCCATGTAGGTCATACTGAGG
CTTTGAAACAAGCAAGAGCACTCGGTGACTATCTCATCGTTGGTGTTCATGATGATAGAG
TAGTTCACGAACAAAAAGGTAGCAACTTCCCAATTATGAATTTACATGAACGTGTACTCT
CTGTACTCTCATGTCGTTATGTCGATGAAGTTGTCATTGGTGCTCCATTCAGTGTCACCA
AGGATATGATTGACTCACTTCACATCAATGTTGTCGTTCATGGTGATGATCAAGTTGTAC
TCGGTCCAGAAGGTGGNGNANANCCANACAAACTCCCAAAANAANTAGGT
Length of 3' end seq. 460
Connected seq. ID VFF530P
Connected seq. 
>VFF530P.Seq
TTGGCCTACTGGAATTAACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATT
AGAGTTTATGTTGATGGTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGA
CAAGCAAGAGAATTAGGAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCA
AAAAATAAAGGTCCACCAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGT
AAATGGGCAGATGAAGTTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGAT
AGTTTAAACTGTGATTTTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAA
GATGTTTATGAAGGCATTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGT
GTTTCAACAACCGAGTTGGTTGGTCGTATGTTATTATGCACCAAAGACCATCTTCAAAAT
GTTTCAGGTGAGCAAACCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAA
TCACCATACACAAGTTTATCACATTTCCTCC----------TGTCAATCCAAACGTTTTA
CACAAACAATCACCATACACAAGTTTATCACATTTCCTCCCAACCACCAGAAAGATCGTC
CAATTCTCAGAAGGTCGTTCACCAAAACCAAACGATAAAATCATTTATATGGATGGTGGT
TTCGATTTATTCCATGTAGGTCATACTGAGGCTTTGAAACAAGCAAGAGCACTCGGTGAC
TATCTCATCGTTGGTGTTCATGATGATAGAGTAGTTCACGAACAAAAAGGTAGCAACTTC
CCAATTATGAATTTACATGAACGTGTACTCTCTGTACTCTCATGTCGTTATGTCGATGAA
GTTGTCATTGGTGCTCCATTCAGTGTCACCAAGGATATGATTGACTCACTTCACATCAAT
GTTGTCGTTCATGGTGATGATCAAGTTGTACTCGGTCCAGAAGGTGGNGNANANCCANAC
AAACTCCCAAAANAANTAGGT
Length of connected seq. 1031
Full length Seq ID VFF530E
Full length Seq. 
>VFF530E.Seq
TTGGCCTACTGGAATTAACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATT
AGAGTTTATGTTGATGGTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGA
CAAGCAAGAGAATTAGGAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCA
AAAAATAAAGGTCCACCAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGT
AAATGGGCAGATGAAGTTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGAT
AGTTTAAACTGTGATTTTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAA
GATGTTTATGAAGGCATTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGT
GTTTCAACAACCGAGTTGGTTGGTCGTATGTTATTATGCACCAAAGACCATCTTCAAAAT
GTTTCAGGTGAGCAAACCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAA
TCACCATACACAAGTTTATCACATTTCCTCCCAACCACCAGAAAGATCGTCCAATTCTCA
GAAGGTCGTTCACCAAAACCAAACGATAAAATCATTTATATGGATGGTGGTTTCGATTTA
TTCCATGTAGGTCATACTGAGGCTTTGAAACAAGCAAGAGCACTCGGTGACTATCTCATC
GTTGGTGTTCATGATGATAGAGTAGTTCACGAACAAAAAGGTAGCAACTTCCCAATTATG
AATTTACATGAACGTGTACTCTCTGTACTCTCATGTCGTTATGTCGATGAAGTTGTCATT
GGTGCTCCATTCAGTGTCACCAAGGATATGATTGACTCACTTCACATCAATGTTGTCGTT
CATGGTGATGATCAAGTTGTACTCGGTCCAGAAGGTGGNGNANANCCANACAAACTCCCA
AAANAANTAGGT
Length of full length seq. 972