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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFF511
Library VF
(Link to library)
Clone ID VFF511
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15456-1
Original site URL
Representative seq. ID VFF511P
(Link to Original site)
Representative DNA sequence 
>VFF511 (VFF511Q) /CSM/VF/VFF5-A/VFF511Q.Seq.d/
ACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACTATT
GTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGA
AGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACAATC
CCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCAGGT
CAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAA
TGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCAAGC
ACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGT
TTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTCGCT
GAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTTAGGT
CTTGCTTTXXXXXXXXXXTTTCGATTTCGCCAAATTCGATGGTATTTTAGGTCTTGCTTT
CCAATCCATCTCTGTTAATTCAATTCCACCAGTCTTTTACAACATGTTATCACAAGGTTT
AGTTTCATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGA
ACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGTGACATTACCTACGTCCCATT
AACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGC
TGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGG
TCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGA
AGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGC
TGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAA
GACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCT
TGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGG
TTTCGCAACTGCCATTCAAGGTTAAATTTTTTTAATTAATTTATATTTAAGATAGAAATA
AAACTAAATAATAGAACAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
t*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGTTI
PISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGASS
TYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIAFDFAKFDGILG
LA---

---FDFAKFDGILGLAFQSISVNSIPPVFYNMLSQGLVSSTLFSFWLSRTPGANGGELSF
GSIDNTKYTGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSLIAGPMA
DITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFVLTPKEYVLEVTEFGKTEC
LSGFMGIELNMGNFWILGDVFISAYYTVFDFGNKQVGFATAIQG*iflinlylr*k*n*i
ieq
Translated Amino Acid sequence (All Frames) 
Frame A:
t*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGTTI
PISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGASS
TYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIAFDFAKFDGILG
LA---

---frfrqirwyfrscfpihlc*fnstsllqhvitrfsfintllllviknsrcqrw*tli
rfnr*hqihw*hylrpinqrnllgiryg*lcyrwsiswflwyylsrnlrfryithcwsng
*yycpq*kircchlkw*rcll*l*ryqhltkcyhhrcws*icfnskrirfrsy*vrkd*m
fewiygyrvkhgkfldpw*cfhlcllycirfw**tswfrnchsrlnffn*fifkieikln
nrt

Frame B:
pdlhkrk*nylf*lyf*lllf*lkl*qyh*tsiklqenleeefhkngqtdyllsmlvpqs
qfqilkmlntmvplplvpqvkpsk*fsilvhptcgfhqrnvqslllhviyitnitavpqa
hmsptelispsntvvvlcqvlslkipsllvh*llkinyslkplpnqvllsispnsmvf*v
ll---

---FDFAKFDGILGLAFQSISVNSIPPVFYNMLSQGLVSSTLFSFWLSRTPGANGGELSF
GSIDNTKYTGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSLIAGPMA
DITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFVLTPKEYVLEVTEFGKTEC
LSGFMGIELNMGNFWILGDVFISAYYTVFDFGNKQVGFATAIQG*iflinlylr*k*n*i
ieq

Frame C:
ltyikeneityfnfifsyycfsssfnstiklpssfkri*kksstkmvkqiicsqcwyhnp
nfrf*rcsilwchyhwyprsslqssfrywfiqlvdsikemsnhcccm*ft*qi*qrclkh
icrqrn*fhhpir*wcyvrfclsrfrhcwfinc*rsiir*shcrtrycfrfrqirwyfrs
cf---

---sispnsmvf*vllsnpslliqfhqsfttcyhkv*fhqhsspsgyqelqvptvvnshs
vqsitpntlvtlptsh*ptkpignslwmtllsmvnqlvsvvllvtqfaiqvhhsllvqwl
illpsmkn*vlss*mvkvsslivalstpyqmlpspllvvnlf*lqkntf*kllsserlnv
*vdlwvss*tweisgslvmfsslltilysilvinklvsqlpfkvkff*liyi*drnktk*
*n
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF511 (VFF511Q) /CSM/VF/VFF5-A/VFF511Q.Seq.d/                       2434   0.0
VFM519 (VFM519Q) /CSM/VF/VFM5-A/VFM519Q.Seq.d/                       1388   0.0
VFL410 (VFL410Q) /CSM/VF/VFL4-A/VFL410Q.Seq.d/                       1388   0.0
VFL274 (VFL274Q) /CSM/VF/VFL2-D/VFL274Q.Seq.d/                       1388   0.0
VFL252 (VFL252Q) /CSM/VF/VFL2-C/VFL252Q.Seq.d/                       1388   0.0
VFG815 (VFG815Q) /CSM/VF/VFG8-A/VFG815Q.Seq.d/                       1388   0.0
VFF141 (VFF141Q) /CSM/VF/VFF1-B/VFF141Q.Seq.d/                       1388   0.0
VFD482 (VFD482Q) /CSM/VF/VFD4-D/VFD482Q.Seq.d/                       1388   0.0
VFD224 (VFD224Q) /CSM/VF/VFD2-A/VFD224Q.Seq.d/                       1388   0.0
VFD137 (VFD137Q) /CSM/VF/VFD1-B/VFD137Q.Seq.d/                       1388   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

Y16962|Y16962.1 Dictyostelium discoideum mRNA for cathepsin D.  1324  0.0  2
AJ243946|AJ243946.1 Dictyostelium discoideum ctsD gene for cathepsin D, exons 1 to 2.  1324  0.0  3
E33916|E33916.1 Candida boidinii strain with lowered protease activity and  utilization thereof as host for producing foreign protein.    36  1e-06  5
AL161500|AL161500.2 Arabidopsis thaliana DNA chromosome 4, contig fragment No. 12.    62  2e-05  1
AL765461|AL765461.1 Arabidopsis thaliana T-DNA flanking sequence GK-139E08-012875.    62  2e-05  1
AV567523|AV567523.1 Arabidopsis thaliana cDNA clone:SQL15g03F, 3' end.    62  2e-05  1
AL762930|AL762930.1 Arabidopsis thaliana T-DNA flanking sequence GK-030C11-011522.    62  2e-05  1
AF372974|AF372974.1 Arabidopsis thaliana AT4g04460/T26N6_7 mRNA, complete cds.    62  2e-05  1
CB264640|CB264640.1 48-E014661-035-002-P12-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000P122Q 5-PRIME, mRNA sequence.    62  2e-05  1
BE525959|BE525959.1 M64C07STM Arabidopsis developing seed Arabidopsis thaliana cDNA clone 600034526R1 5', mRNA sequence.    62  2e-05  1
dna update 2003. 9.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF454831_1(AF454831|pid:none) Apriona germari cathepsin D mRNA, ...   181   1e-88
DQ868657_1(DQ868657|pid:none) Cucumis sativus aspartic proteinas...   183   1e-80
(Q805F2) RecName: Full=Cathepsin E-B;          EC=3.4.23.34; Fla...   188   5e-79
BC161297_1(BC161297|pid:none) Xenopus tropicalis hypothetical pr...   189   6e-79
(P16228) RecName: Full=Cathepsin E;          EC=3.4.23.34; Flags...   188   9e-78
Y10928_1(Y10928|pid:none) M.musculus gene encoding cathepsin E.       184   4e-76
(P70269) RecName: Full=Cathepsin E;          EC=3.4.23.34; Flags...   184   5e-76
EF676352_1(EF676352|pid:none) Picea sitchensis clone WS02732_P05...   159   2e-75
EF678577_1(EF678577|pid:none) Picea sitchensis clone WS02928_J09...   159   2e-75
FN357346_14(FN357346|pid:none) Schistosoma mansoni genome sequen...   162   7e-74
protein update 2009. 6.19
PSORT 

 psg: 0.98  gvh: 0.77  alm: 0.38  top: 0.57  tms: 0.00  mit: 0.36  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: extracellular, including cell wall
	 20.0 %: mitochondrial
	 16.0 %: nuclear
	 12.0 %: vacuolar
	  4.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for VFF511 is exc
5' end seq. ID VFF511F
5' end seq. 
>VFF511F.Seq
ACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACTATT
GTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGA
AGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACAATC
CCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCAGGT
CAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAA
TGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCAAGC
ACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGT
TTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTCGCT
GAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTTAGGT
CTTGCTTT----------
Length of 5' end seq. 548
3' end seq. ID VFF511Z
3' end seq. 
>VFF511Z.Seq
----------TTTCGATTTCGCCAAATTCGATGGTATTTTAGGTCTTGCTTTCCAATCCA
TCTCTGTTAATTCAATTCCACCAGTCTTTTACAACATGTTATCACAAGGTTTAGTTTCAT
CAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCAT
TCGGTTCAATCGATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACG
AAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCT
GTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGG
CTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCT
TCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTG
AATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAAT
GTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATG
TTTTCATCTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAA
CTGCCATTCAAGGTTAAATTTTTTTAATTAATTTATATTTAAGATAGAAATAAAACTAAA
TAATAGAACAA
Length of 3' end seq. 721
Connected seq. ID VFF511P
Connected seq. 
>VFF511P.Seq
ACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACTATT
GTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGA
AGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACAATC
CCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCAGGT
CAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAA
TGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCAAGC
ACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGT
TTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTCGCT
GAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTTAGGT
CTTGCTTT----------TTTCGATTTCGCCAAATTCGATGGTATTTTAGGTCTTGCTTT
CCAATCCATCTCTGTTAATTCAATTCCACCAGTCTTTTACAACATGTTATCACAAGGTTT
AGTTTCATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGA
ACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGTGACATTACCTACGTCCCATT
AACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGC
TGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGG
TCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGA
AGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGC
TGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAA
GACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCT
TGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGG
TTTCGCAACTGCCATTCAAGGTTAAATTTTTTTAATTAATTTATATTTAAGATAGAAATA
AAACTAAATAATAGAACAA
Length of connected seq. 1269
Full length Seq ID -
Full length Seq. -
Length of full length seq. -