>VFF413 (VFF413Q) /CSM/VF/VFF4-A/VFF413Q.Seq.d/
ATAAAATAATAGTTAATATAGCTAAATTATAGTTATAATTTTTAAAAAAAATATGAAATT
AATTTCAATAATTTTTTCCTTATTTTTAATAATCAATTTGGTTTTTTTCTACAGATCCAA
CAATTGTTAGTTTTAGAAAGCCAGTTGAAAAAGTATATCCAAGTGAATATTGTTTGCCAT
CTTTCACTATATTGATTAATTATCCAGATCCAATCCAATATAATNATCAATTAGGTTTAT
ATGATAGGAGTGGAGGGTATGGTGGCAGCATGCCATCTGCCGTAAATGGAACCTTANTTC
TTTTTACACTTACACATAAAGTTCCAATTGGTAAAAATCAACCAGTTTCTTTGTATATCC
AAGATACCTCAAAAAATCCATGGTTTACTTTTGATTTCAATAACTATACTACCTATGATT
GCGATCCACTTCCACCCTGTTACTATGAAATCAAAACAAAATACTTGGATTAAAACTAGT
GGCCAATATTCCAATTTAATTTGGTACCATATGGTTGAAGTTTCAGGTTTAGATAAAXXX
XXXXXXXACTTAATAGTCTTTTTGATGGTTTAAGTGCACCAGCAAACCCACCTGTTTCAA
TTAGATCCTTAGAAAATTATCCAATCAATGGTTCAACTGTTACTAAAAATGAAATGGTTA
ATGATGCAATGTATATTATGAATATCATAAACAAAAATGCTCTTTGTGGTATTTCATCAC
CAAAAGATTTTTTGATTTCAAATATATTAATCCCAGTTTTAGGTACACCTGCAAATGCTT
CTTACATTGGTTTCTCATCTTTTAACTATCAAAATAGTCCAATTAAATCCCATGTTCAGA
TCATTGATAGTCAAGGTACAAAAAGAATTGGTATTGATAATGTGCTTTTTGTTAATCCAA
AATCTTATGATAATGTCATTGAAAATGTTCAAAATGCAACCTATGTTAAAAGCTCTAATA
TTAATAAAACTTTAATTAATATTAAGGCTGATGTTTCATTTAATCCAAAAGCTACTTTAA
CAAGTAGCTTTTCAACTTCTTTATACCAAACAGTGTTAGCATATCCATATGGTTATTCAA
ATGGTACAGTTAAATACCATTCAGTTTCATTGTCATCTGCATCTCCAGAGTATTCCACTT
CTTCAAATTCATATATAATTTTAGGCTCTCCATCNAAATCGGATCCGGTAATTCATCACA
ATCACAG

ik**li*lnysynf*kkyeinfnnfflifnnqfgfflqiqqllvlesqlkkyiqvnivch
lsly*liiqiqsnixin*vymigvegmvaachlp*MEPXFFLHLHIKFQLVKINQFLCIS
KIPQKIHGLLLISITILPMIAIHFHPVTMKSKQNTWIKTSGQYSNLIWYHMVEVSGLDK-
--

---LNSLFDGLSAPANPPVSIRSLENYPINGSTVTKNEMVNDAMYIMNIINKNALCGISS
PKDFLISNILIPVLGTPANASYIGFSSFNYQNSPIKSHVQIIDSQGTKRIGIDNVLFVNP
KSYDNVIENVQNATYVKSSNINKTLINIKADVSFNPKATLTSSFSTSLYQTVLAYPYGYS
NGTVKYHSVSLSSASPEYSTSSNSYIILGSPSKSDPVIHHNH

Frame A:
ik**li*lnysynf*kkyeinfnnfflifnnqfgfflqiqqllvlesqlkkyiqvnivch
lsly*liiqiqsnixin*vymigvegmvaachlp*MEPXFFLHLHIKFQLVKINQFLCIS
KIPQKIHGLLLISITILPMIAIHFHPVTMKSKQNTWIKTSGQYSNLIWYHMVEVSGLDK-
--

---t**sf*wfkctskptcfn*ilrklsnqwfncy*k*ng**cnvyyeyhkqkcslwyfi
tkrffdfkyinpsfrytckcflhwflif*lsk*sn*ipcsdh**srykknwy**cafc*s
kil**ch*kcskcnlc*kl*y**nfn*y*g*cfi*sksyfnk*lfnffipnsvsisiwlf
kwys*ipfsfivicisrvfhffkfiynfrlsixigsgnssqsq

Frame B:
*nns*ys*iiviifkknmklisiifslfliinlvffyrsnnc*f*kas*ksisk*ilfai
fhyid*lsrsnpi*xsirfi**ewrvwwqhaicrkwnlxsfytyt*ssnw*kstsffvyp
rylkksmvyf*fq*lyyl*lrststlll*nqnkilglklvanipi*fgtiwlkfqv*i--
-

---LNSLFDGLSAPANPPVSIRSLENYPINGSTVTKNEMVNDAMYIMNIINKNALCGISS
PKDFLISNILIPVLGTPANASYIGFSSFNYQNSPIKSHVQIIDSQGTKRIGIDNVLFVNP
KSYDNVIENVQNATYVKSSNINKTLINIKADVSFNPKATLTSSFSTSLYQTVLAYPYGYS
NGTVKYHSVSLSSASPEYSTSSNSYIILGSPSKSDPVIHHNH

Frame C:
kiivniakl*l*flkki*n*fq*ffpyf**siwffstdptivsfrkpvekvypseyclps
ftilinypdpiqynxqlglydrsggyggsmpsavngtlxlftlthkvpigknqpvslyiq
dtsknpwftfdfnnyttydcdplppcyyeiktkyld*n*wpifqfnlvpyg*sfrfr*--
-

---livflmv*vhqqthlfqldp*kiiqsmvqlllkmkwlmmqcil*is*tkmlfvvfhh
qkif*fqiy*sqf*vhlqmlltlvshlltikivqlnpmfrslivkvqkelvlimcflliq
nlmimslkmfkmqpmlkalilikl*lilrlmfhliqkll*qvafqllytkqc*hihmviq
mvqlntiqfhchlhlqsipllqihi*f*alhxnrir*fitit

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF413 (VFF413Q) /CSM/VF/VFF4-A/VFF413Q.Seq.d/                       2101   0.0
VHQ313 (VHQ313Q) /CSM/VH/VHQ3-A/VHQ313Q.Seq.d/                        815   0.0
VHK829 (VHK829Q) /CSM/VH/VHK8-B/VHK829Q.Seq.d/                        815   0.0
VHG314 (VHG314Q) /CSM/VH/VHG3-A/VHG314Q.Seq.d/                        799   0.0
VFE507 (VFE507Q) /CSM/VF/VFE5-A/VFE507Q.Seq.d/                         42   0.020
SSD241 (SSD241Q) /CSM/SS/SSD2-B/SSD241Q.Seq.d/                         42   0.020
SSA810 (SSA810Q) /CSM/SS/SSA8-A/SSA810Q.Seq.d/                         42   0.020
SLI447 (SLI447Q) /CSM/SL/SLI4-B/SLI447Q.Seq.d/                         42   0.020
SLI155 (SLI155Q) /CSM/SL/SLI1-C/SLI155Q.Seq.d/                         42   0.020
SLF548 (SLF548Q) /CSM/SL/SLF5-B/SLF548Q.Seq.d/                         42   0.020

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF129501|AF129501.1 Buchnera aphidicola natural-host Diuraphis noxia acetohydroxy acid synthase large subunit (ilvI) and acetohydroxy acid synthase small subunit (ilvH) genes, complete cds; and unknown genes.    42  0.12  2
AZ691992|AZ691992.1 ENTHT05TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    42  0.58  2
AC126972|AC126972.4 Rattus norvegicus clone CH230-133N10, *** SEQUENCING IN PROGRESS ***, 10 unordered pieces.    46  1.0  1
BH400246|BH400246.1 AG-ND-126M3.TR ND-TAM Anopheles gambiae genomic clone AG-ND-126M3, DNA sequence.    46  1.0  1
AC097618|AC097618.5 Rattus norvegicus clone CH230-112J18, *** SEQUENCING IN PROGRESS ***, 9 unordered pieces.    46  1.0  1
AC022204|AC022204.4 Homo sapiens chromosome 8 clone RP11-535F5, WORKING DRAFT SEQUENCE, 8 unordered pieces.    46  1.3  4
AF083031|AF083031.2 Guillardia theta nucleomorph chromosome 3, complete sequence.    34  1.3  5
AF016447|AF016447.2 Caenorhabditis elegans cosmid C54F6, complete sequence.    46  1.4  2
CD769067|CD769067.1 AGENCOURT_14716832 NICHD_MM_Hyp1 Mus musculus cDNA clone IMAGE:6974989 5', mRNA sequence.    38  1.6  2
AC114945|AC114945.2 Homo sapiens chromosome 5 clone CTD-2604I2, complete sequence.    38  1.7  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM295005_1(AM295005|pid:none) Homo sapiens mRNA for boll protein.      42   0.033
AJ717405_1(AJ717405|pid:none) Pan paniscus partial mRNA for BOUL...    42   0.033
AK093453_1(AK093453|pid:none) Homo sapiens cDNA FLJ36134 fis, cl...    42   0.033
AB074454_1(AB074454|pid:none) Macaca fascicularis testis cDNA cl...    42   0.033
AJ746579_1(AJ746579|pid:none) Microcebus murinus partial mRNA fo...    42   0.033
(Q8N9W6) RecName: Full=Protein boule-like;  &AC011997_1(AC011997...    42   0.033
FJ623266_1(FJ623266|pid:none) Bos taurus boule mRNA, complete cds.     42   0.043
AJ717407_1(AJ717407|pid:none) Callithrix jacchus partial mRNA fo...    41   0.096
AJ717408_1(AJ717408|pid:none) Saimiri sciureus partial mRNA for ...    41   0.096
AE014187_412(AE014187|pid:none) Plasmodium falciparum 3D7 chromo...    38   0.62

 psg: 0.75  gvh: 0.48  alm: 0.24  top: 0.57  tms: 0.07  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.02  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for VFF413 is nuc

>VFF413F.Seq
ATAAAATAATAGTTAATATAGCTAAATTATAGTTATAATTTTTAAAAAAAATATGAAATT
AATTTCAATAATTTTTTCCTTATTTTTAATAATCAATTTGGTTTTTTTCTACAGATCCAA
CAATTGTTAGTTTTAGAAAGCCAGTTGAAAAAGTATATCCAAGTGAATATTGTTTGCCAT
CTTTCACTATATTGATTAATTATCCAGATCCAATCCAATATAATNATCAATTAGGTTTAT
ATGATAGGAGTGGAGGGTATGGTGGCAGCATGCCATCTGCCGTAAATGGAACCTTANTTC
TTTTTACACTTACACATAAAGTTCCAATTGGTAAAAATCAACCAGTTTCTTTGTATATCC
AAGATACCTCAAAAAATCCATGGTTTACTTTTGATTTCAATAACTATACTACCTATGATT
GCGATCCACTTCCACCCTGTTACTATGAAATCAAAACAAAATACTTGGATTAAAACTAGT
GGCCAATATTCCAATTTAATTTGGTACCATATGGTTGAAGTTTCAGGTTTAGATAAA---
-------

>VFF413Z.Seq
----------ACTTAATAGTCTTTTTGATGGTTTAAGTGCACCAGCAAACCCACCTGTTT
CAATTAGATCCTTAGAAAATTATCCAATCAATGGTTCAACTGTTACTAAAAATGAAATGG
TTAATGATGCAATGTATATTATGAATATCATAAACAAAAATGCTCTTTGTGGTATTTCAT
CACCAAAAGATTTTTTGATTTCAAATATATTAATCCCAGTTTTAGGTACACCTGCAAATG
CTTCTTACATTGGTTTCTCATCTTTTAACTATCAAAATAGTCCAATTAAATCCCATGTTC
AGATCATTGATAGTCAAGGTACAAAAAGAATTGGTATTGATAATGTGCTTTTTGTTAATC
CAAAATCTTATGATAATGTCATTGAAAATGTTCAAAATGCAACCTATGTTAAAAGCTCTA
ATATTAATAAAACTTTAATTAATATTAAGGCTGATGTTTCATTTAATCCAAAAGCTACTT
TAACAAGTAGCTTTTCAACTTCTTTATACCAAACAGTGTTAGCATATCCATATGGTTATT
CAAATGGTACAGTTAAATACCATTCAGTTTCATTGTCATCTGCATCTCCAGAGTATTCCA
CTTCTTCAAATTCATATATAATTTTAGGCTCTCCATCNAAATCGGATCCGGTAATTCATC
ACAATCACAG

>VFF413P.Seq
ATAAAATAATAGTTAATATAGCTAAATTATAGTTATAATTTTTAAAAAAAATATGAAATT
AATTTCAATAATTTTTTCCTTATTTTTAATAATCAATTTGGTTTTTTTCTACAGATCCAA
CAATTGTTAGTTTTAGAAAGCCAGTTGAAAAAGTATATCCAAGTGAATATTGTTTGCCAT
CTTTCACTATATTGATTAATTATCCAGATCCAATCCAATATAATNATCAATTAGGTTTAT
ATGATAGGAGTGGAGGGTATGGTGGCAGCATGCCATCTGCCGTAAATGGAACCTTANTTC
TTTTTACACTTACACATAAAGTTCCAATTGGTAAAAATCAACCAGTTTCTTTGTATATCC
AAGATACCTCAAAAAATCCATGGTTTACTTTTGATTTCAATAACTATACTACCTATGATT
GCGATCCACTTCCACCCTGTTACTATGAAATCAAAACAAAATACTTGGATTAAAACTAGT
GGCCAATATTCCAATTTAATTTGGTACCATATGGTTGAAGTTTCAGGTTTAGATAAA---
-------ACTTAATAGTCTTTTTGATGGTTTAAGTGCACCAGCAAACCCACCTGTTTCAA
TTAGATCCTTAGAAAATTATCCAATCAATGGTTCAACTGTTACTAAAAATGAAATGGTTA
ATGATGCAATGTATATTATGAATATCATAAACAAAAATGCTCTTTGTGGTATTTCATCAC
CAAAAGATTTTTTGATTTCAAATATATTAATCCCAGTTTTAGGTACACCTGCAAATGCTT
CTTACATTGGTTTCTCATCTTTTAACTATCAAAATAGTCCAATTAAATCCCATGTTCAGA
TCATTGATAGTCAAGGTACAAAAAGAATTGGTATTGATAATGTGCTTTTTGTTAATCCAA
AATCTTATGATAATGTCATTGAAAATGTTCAAAATGCAACCTATGTTAAAAGCTCTAATA
TTAATAAAACTTTAATTAATATTAAGGCTGATGTTTCATTTAATCCAAAAGCTACTTTAA
CAAGTAGCTTTTCAACTTCTTTATACCAAACAGTGTTAGCATATCCATATGGTTATTCAA
ATGGTACAGTTAAATACCATTCAGTTTCATTGTCATCTGCATCTCCAGAGTATTCCACTT
CTTCAAATTCATATATAATTTTAGGCTCTCCATCNAAATCGGATCCGGTAATTCATCACA
ATCACAG