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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFF395
Library VF
(Link to library)
Clone ID VFF395
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16219-1
Original site URL
Representative seq. ID VFF395P
(Link to Original site)
Representative DNA sequence 
>VFF395 (VFF395Q) /CSM/VF/VFF3-D/VFF395Q.Seq.d/
AATATGTTTGAAGCACAACCACAATTTTTAAATATATTCAATCATTCAAATCAAAGAAAT
CAGAAACAACCGGTGGCTTTGGCCAATACAATTTTACAATCTGCAATTCATATTGAAAAA
TTAAATGAAATCAATTTAATGCCAATCGTTCATAAACACGTTGCATTAGGTATTACACCT
GAAATGTATCCAATTGTTGGTGCTCATTTATTGGGTGCTATGAAAACTGTAATGCAAGAT
GAAGCAACTCCTGAAATTATGGCTGCATGGACTGAGGCATATAGAGCAGTTGCACAAGCA
TTTATGGATGCAGAAGAAGATTTATACTTTGAGACTGAGGAACAAATTGGTGGTTGGAAA
GATACGAGAGAGTTTGTAGTTGATAGAATTGAAGAAGAGACACCATTAATTAAATCATTT
TACTTTAAAGCATATGATGGTAAAGAGATAGCTACTTATATTCCTGGACAATATATTACT
GTAAAGATTACATTACCGGGTGATGGTGTTTGATGTACCAACTGATAAGXXXXXXXXXXT
GATGGTGTTGATGTACCAACTGATAAGATGAGAACTTATGTCAGACATTACAGTTTATCA
GATAAACCAAATGATGAATACTATCGTATTTCAATAAAGAAAGAACTCGGTAAGAATACA
CCAAATGGTATAGTATCAAATCATTTTCATAATAATATTAAAGTGGGTGATGTAGTACCA
ATGTCGGTCCCAGCAGGCGATTTCGTGGTAAATAACGATTCTGAGACTCCAATCCTTTTA
ATTTGTGGTGGTGTTGGTATTAACCCGTTATTTAGTATGCTTAAAGAGATATTGGTCCAA
CAACCAGATAGAAAAATAAATTTCATTTTCTCAACTCATTGCGAATCAAGTCAACCATTT
AAAGAGGAACTTAAACAATTGGAAGACGATTATAAAGAAACTGGTAATCTTAAAATCAAT
TTAGTATACTCTGAAAATCAAGGTCATATCAATAAAGAAATCATTGAAAAATATTCAACT
CAACATGTTGATCAAGCTGAAATCGCTGAAACCGACGTTTACATTTGTGGTCCTGTACCT
TTTATGATGCAAGTTAACAAAGATTTATTACAATTAGGTTTCCATAAAGAAAATGTACAT
TATGAATTATTTGGTCCATTAACTCCAGTCCTCGAAGAAAATCAAATGTTAAGAGGTGTA
AAAAATATTATTAAAATTATTTAAATAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
iclkhnhnf*iysiiqikeirnnrwlwpiqfynlqfilkn*mksi*cqsfintlh*vlhl
kciqllvliywvl*kl*ckmkqllklwlhglrhieqlhkhlwmqkkiytlrlrnklvvgk
iresl*lielkkrhh*lnhftlkhmmvkr*llifldnill*RLHYRVMVFDVPTDK---

---DGVDVPTDKMRTYVRHYSLSDKPNDEYYRISIKKELGKNTPNGIVSNHFHNNIKVGD
VVPMSVPAGDFVVNNDSETPILLICGGVGINPLFSMLKEILVQQPDRKINFIFSTHCESS
QPFKEELKQLEDDYKETGNLKINLVYSENQGHINKEIIEKYSTQHVDQAEIAETDVYICG
PVPFMMQVNKDLLQLGFHKENVHYELFGPLTPVLEENQMLRGVKNIIKII*i
Translated Amino Acid sequence (All Frames) 
Frame A:
nmfeaqpqflnifnhsnqrnqkqpvalantilqsaihieklneinlmpivhkhvalgitp
emypivgahllgamktvmqdeatpeimaawteayravaqafmdaeedlyfeteeqiggwk
dtrefvvdrieeetpliksfyfkaydgkeiatyipgqyitvkitlpgdgv*ctn**---

---*wc*ctn**denlcqtlqfir*tk**ilsyfnkertr*eytkwysiksfs**y*sg*
cstnvgpsrrfrgk*rf*dsnpfnlwwcwy*pvi*ya*rdigpttr*knkfhflnslrik
sti*rgt*tigrrl*rnw*s*nqfsil*ksrsyq*rnh*kifnstc*ss*nr*nrrlhlw
sctfydas*qrfitirfp*rkctl*iiwsinssprrksnvkrckkyy*nylnk

Frame B:
iclkhnhnf*iysiiqikeirnnrwlwpiqfynlqfilkn*mksi*cqsfintlh*vlhl
kciqllvliywvl*kl*ckmkqllklwlhglrhieqlhkhlwmqkkiytlrlrnklvvgk
iresl*lielkkrhh*lnhftlkhmmvkr*llifldnill*RLHYRVMVFDVPTDK---

---DGVDVPTDKMRTYVRHYSLSDKPNDEYYRISIKKELGKNTPNGIVSNHFHNNIKVGD
VVPMSVPAGDFVVNNDSETPILLICGGVGINPLFSMLKEILVQQPDRKINFIFSTHCESS
QPFKEELKQLEDDYKETGNLKINLVYSENQGHINKEIIEKYSTQHVDQAEIAETDVYICG
PVPFMMQVNKDLLQLGFHKENVHYELFGPLTPVLEENQMLRGVKNIIKII*i

Frame C:
yv*stttifkyiqsfkskksettggfgqynfticnsy*kik*nqfnanrs*trciryyt*
nvsncwcsfigcyencnar*sns*nygcmd*gi*ssctsiygcrrrfil*d*gtnwwler
yervcs**n*rrdtin*iill*si*w*rdsylyswtiyyckdyitg*wclmyqli---

---mvlmyqlir*elmsditvyqinqmmntivfq*rknsvrihqmv*yqiifiiilkwvm
*yqcrsqqaisw*itilrlqsf*fvvvlvltrylvclkrywsnnqiek*isfsqlianqv
nhlkrnlnnwktiikklvilksi*ytlkikvisikkslkniqlnmliklkslkptftfvv
lyll*ckltkiyyn*vsikkmyimnylvh*lqsskkikc*ev*killklfk*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF395 (VFF395Q) /CSM/VF/VFF3-D/VFF395Q.Seq.d/                       2377   0.0
SSC690 (SSC690Q) /CSM/SS/SSC6-D/SSC690Q.Seq.d/                       1302   0.0
VSD112 (VSD112Q) /CSM/VS/VSD1-A/VSD112Q.Seq.d/                       1300   0.0
VFA836 (VFA836Q) /CSM/VF/VFA8-B/VFA836Q.Seq.d/                       1241   0.0
VFA486 (VFA486Q) /CSM/VF/VFA4-D/VFA486Q.Seq.d/                       1211   0.0
CFA206 (CFA206Q) /CSM/CF/CFA2-A/CFA206Q.Seq.d/                       1197   0.0
FC-BK04 (FC-BK04Q) /CSM/FC/FC-BK/FC-BK04Q.Seq.d/                     1195   0.0
VFK704 (VFK704Q) /CSM/VF/VFK7-A/VFK704Q.Seq.d/                       1124   0.0
VFH794 (VFH794Q) /CSM/VF/VFH7-D/VFH794Q.Seq.d/                       1104   0.0
VHK671 (VHK671Q) /CSM/VH/VHK6-C/VHK671Q.Seq.d/                       1096   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AB025584|AB025584.1 Dictyostelium discoideum DdFHb mRNA for flavohemoglobin, complete cds.  1318  0.0  2
AB025583|AB025583.1 Dictyostelium discoideum DdFHa mRNA for flavohemoglobin, complete cds.    80  5e-50  8
AC006279|AC006279.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    46  0.001  13
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    50  0.002  13
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    34  0.012  13
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.          38  0.020  12
AE014831|AE014831.1 Plasmodium falciparum 3D7 chromosome 10 section 3 of 7 of the complete sequence.    38  0.029  10
AE014852|AE014852.2 Plasmodium falciparum 3D7 chromosome 12, section 9 of 9 of the complete sequence.    46  0.039  10
AX344573|AX344573.1 Sequence 24 from Patent WO0200932.            50  0.066  1
AC116330|AC116330.2 Dictyostelium discoideum chromosome 2 map 3191214-3323468 strain AX4, complete sequence.    32  0.080  10
dna update 2003. 9.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB025584_1(AB025584|pid:none) Dictyostelium discoideum DdFHb mRN...   781   0.0
CP001186_1337(CP001186|pid:none) Bacillus cereus G9842, complete...   294   5e-78
CP000903_1350(CP000903|pid:none) Bacillus weihenstephanensis KBA...   292   1e-77
CP001407_1354(CP001407|pid:none) Bacillus cereus 03BB102, comple...   292   2e-77
(Q6HLA6) RecName: Full=Flavohemoprotein; AltName: Full=Hemoglobi...   291   2e-77
(Q73B49) RecName: Full=Flavohemoprotein; AltName: Full=Hemoglobi...   290   7e-77
(Q81FW4) RecName: Full=Flavohemoprotein; AltName: Full=Hemoglobi...   290   7e-77
CP000485_1227(CP000485|pid:none) Bacillus thuringiensis str. Al ...   290   7e-77
CP001176_1375(CP001176|pid:none) Bacillus cereus B4264, complete...   290   9e-77
(Q81T23) RecName: Full=Flavohemoprotein; AltName: Full=Hemoglobi...   289   1e-76
protein update 2009. 6.19
PSORT 

 psg: 0.55  gvh: 0.21  alm: 0.30  top: 0.80  tms: 0.07  mit: 0.35  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.07  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: nuclear
	 24.0 %: mitochondrial
	 24.0 %: cytoplasmic
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VFF395 is nuc
5' end seq. ID VFF395F
5' end seq. 
>VFF395F.Seq
AATATGTTTGAAGCACAACCACAATTTTTAAATATATTCAATCATTCAAATCAAAGAAAT
CAGAAACAACCGGTGGCTTTGGCCAATACAATTTTACAATCTGCAATTCATATTGAAAAA
TTAAATGAAATCAATTTAATGCCAATCGTTCATAAACACGTTGCATTAGGTATTACACCT
GAAATGTATCCAATTGTTGGTGCTCATTTATTGGGTGCTATGAAAACTGTAATGCAAGAT
GAAGCAACTCCTGAAATTATGGCTGCATGGACTGAGGCATATAGAGCAGTTGCACAAGCA
TTTATGGATGCAGAAGAAGATTTATACTTTGAGACTGAGGAACAAATTGGTGGTTGGAAA
GATACGAGAGAGTTTGTAGTTGATAGAATTGAAGAAGAGACACCATTAATTAAATCATTT
TACTTTAAAGCATATGATGGTAAAGAGATAGCTACTTATATTCCTGGACAATATATTACT
GTAAAGATTACATTACCGGGTGATGGTGTTTGATGTACCAACTGATAAG----------
Length of 5' end seq. 529
3' end seq. ID VFF395Z
3' end seq. 
>VFF395Z.Seq
----------TGATGGTGTTGATGTACCAACTGATAAGATGAGAACTTATGTCAGACATT
ACAGTTTATCAGATAAACCAAATGATGAATACTATCGTATTTCAATAAAGAAAGAACTCG
GTAAGAATACACCAAATGGTATAGTATCAAATCATTTTCATAATAATATTAAAGTGGGTG
ATGTAGTACCAATGTCGGTCCCAGCAGGCGATTTCGTGGTAAATAACGATTCTGAGACTC
CAATCCTTTTAATTTGTGGTGGTGTTGGTATTAACCCGTTATTTAGTATGCTTAAAGAGA
TATTGGTCCAACAACCAGATAGAAAAATAAATTTCATTTTCTCAACTCATTGCGAATCAA
GTCAACCATTTAAAGAGGAACTTAAACAATTGGAAGACGATTATAAAGAAACTGGTAATC
TTAAAATCAATTTAGTATACTCTGAAAATCAAGGTCATATCAATAAAGAAATCATTGAAA
AATATTCAACTCAACATGTTGATCAAGCTGAAATCGCTGAAACCGACGTTTACATTTGTG
GTCCTGTACCTTTTATGATGCAAGTTAACAAAGATTTATTACAATTAGGTTTCCATAAAG
AAAATGTACATTATGAATTATTTGGTCCATTAACTCCAGTCCTCGAAGAAAATCAAATGT
TAAGAGGTGTAAAAAATATTATTAAAATTATTTAAATAAA
Length of 3' end seq. 690
Connected seq. ID VFF395P
Connected seq. 
>VFF395P.Seq
AATATGTTTGAAGCACAACCACAATTTTTAAATATATTCAATCATTCAAATCAAAGAAAT
CAGAAACAACCGGTGGCTTTGGCCAATACAATTTTACAATCTGCAATTCATATTGAAAAA
TTAAATGAAATCAATTTAATGCCAATCGTTCATAAACACGTTGCATTAGGTATTACACCT
GAAATGTATCCAATTGTTGGTGCTCATTTATTGGGTGCTATGAAAACTGTAATGCAAGAT
GAAGCAACTCCTGAAATTATGGCTGCATGGACTGAGGCATATAGAGCAGTTGCACAAGCA
TTTATGGATGCAGAAGAAGATTTATACTTTGAGACTGAGGAACAAATTGGTGGTTGGAAA
GATACGAGAGAGTTTGTAGTTGATAGAATTGAAGAAGAGACACCATTAATTAAATCATTT
TACTTTAAAGCATATGATGGTAAAGAGATAGCTACTTATATTCCTGGACAATATATTACT
GTAAAGATTACATTACCGGGTGATGGTGTTTGATGTACCAACTGATAAG----------T
GATGGTGTTGATGTACCAACTGATAAGATGAGAACTTATGTCAGACATTACAGTTTATCA
GATAAACCAAATGATGAATACTATCGTATTTCAATAAAGAAAGAACTCGGTAAGAATACA
CCAAATGGTATAGTATCAAATCATTTTCATAATAATATTAAAGTGGGTGATGTAGTACCA
ATGTCGGTCCCAGCAGGCGATTTCGTGGTAAATAACGATTCTGAGACTCCAATCCTTTTA
ATTTGTGGTGGTGTTGGTATTAACCCGTTATTTAGTATGCTTAAAGAGATATTGGTCCAA
CAACCAGATAGAAAAATAAATTTCATTTTCTCAACTCATTGCGAATCAAGTCAACCATTT
AAAGAGGAACTTAAACAATTGGAAGACGATTATAAAGAAACTGGTAATCTTAAAATCAAT
TTAGTATACTCTGAAAATCAAGGTCATATCAATAAAGAAATCATTGAAAAATATTCAACT
CAACATGTTGATCAAGCTGAAATCGCTGAAACCGACGTTTACATTTGTGGTCCTGTACCT
TTTATGATGCAAGTTAACAAAGATTTATTACAATTAGGTTTCCATAAAGAAAATGTACAT
TATGAATTATTTGGTCCATTAACTCCAGTCCTCGAAGAAAATCAAATGTTAAGAGGTGTA
AAAAATATTATTAAAATTATTTAAATAAA
Length of connected seq. 1219
Full length Seq ID -
Full length Seq. -
Length of full length seq. -