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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFF382
Library VF
(Link to library)
Clone ID VFF382
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12898-1
Original site URL
Representative seq. ID VFF382P
(Link to Original site)
Representative DNA sequence 
>VFF382 (VFF382Q) /CSM/VF/VFF3-D/VFF382Q.Seq.d/
AAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTAGAGTTTATGTTGATGGTT
GTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGACAAGCAAGAGAATTAGGAG
ATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAAAAAATAAAGGTCCACCAG
TTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTAAATGGGCAGATGAAGTTG
CAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATAGTTTAAACTGTGATTTTT
GTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAGATGTTTATGAAGGCATTA
AAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTGTTTCAACAACCGAGTTGG
TTGGTCGTATGTTATTATGCACCAAAGACCATCTTCAAAATGTTTCAGGTGAGCAAACCT
CACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAATCACCATACACAAGTTTAT
CACATTTNCXXXXXXXXXXTCATGTCGTTANGTCGATGAAGTTGTCATTGGTGCTCCATT
CAGTGTCNCCAAAAATATGATTGACTCNCTTCNCATCAATGTTGTCGTTCATGGTGATGA
TCAAGTTGNACTCGGTCCANAAGGTGGTGTANATCCATACAAACTCCCAAAANAATTAGG
NATTTACAAANAAGTTNAACANACTGAAGGTCNCNCTGCAACTGAAATCGTTAAAANAAT
CATTGATAATAGACTTCAANATGAAGCTCGNAATCGTAAAAAANAAGCNAAANAAATTAA
TTTCNTTGAACNATCNAATTAAAAAAAATTAAAAAATAAAAAATAAAAATAAAACNAATA
AAAAAAAAAAAATAGAAAAAAAATTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
VFTMSTTTNKKPIRVYVDGCFDLMHFGHANALRQARELGDILVVGVHTDEEIAKNKGPPV
MNEQERYKAVRACKWADEVAEGAPYTLTEEYLDSLNCDFCVHGEDISVGADGKDVYEGIK
KSGKFRFIKRTEGVSTTELVGRMLLCTKDHLQNVSGEQTSPLGGVNPNVLHKQSPYTSLS
HX---

---SCRXVDEVVIGAPFSVXKNMIDSLXINVVVHGDDQVXLGPXGGVXPYKLPKXLGIYK
XVXXTEGXXATEIVKXIIDNRLQXEARNRKKXAKXINFXEXSN*kklknkk*k*nx*kkk
nrkki
Translated Amino Acid sequence (All Frames) 
Frame A:
kflq*vlqliksqlefmlmvvli*cildmqmh*dkqen*eif**lvyiqmkklqkikvhq
l*mnkkdikqyvhvngqmklqkvhlil*lkniwiv*tvifvymvkifqlvqmvkmfmkal
krvvnldssnvlkvfqqpswlvvcyyapktifkmfqvskphhwevsiqtfytnnhhtqvy
hix---

---SCRXVDEVVIGAPFSVXKNMIDSLXINVVVHGDDQVXLGPXGGVXPYKLPKXLGIYK
XVXXTEGXXATEIVKXIIDNRLQXEARNRKKXAKXINFXEXSN*kklknkk*k*nx*kkk
nrkki

Frame B:
sfyneyyn**kan*slc*wlf*fnafwtckciktskrirryfsswctyr*rnckk*rsts
ye*trki*sstcm*mgr*scrrctlyfn*rifg*fkl*flctw*ryfswcrw*rcl*rh*
kew*i*ihqty*rcfnnrvgwsyvimhqrpsskcfr*anltigrcqskrftqtitihkfi
tf---

---hvvxsmklslvlhsvspki*ltxfxsmlsfmvmiklxsvxkvvxihtnsqxn*xftx
kxnxlkvxlqlkslkxsliidfxmklxivkxkxxklislnxxikkn*kiknknktnkkkk
iekkf

Frame C:
VFTMSTTTNKKPIRVYVDGCFDLMHFGHANALRQARELGDILVVGVHTDEEIAKNKGPPV
MNEQERYKAVRACKWADEVAEGAPYTLTEEYLDSLNCDFCVHGEDISVGADGKDVYEGIK
KSGKFRFIKRTEGVSTTELVGRMLLCTKDHLQNVSGEQTSPLGGVNPNVLHKQSPYTSLS
HX---

---mslxr*schwcsiqcxqkyd*lxshqccrsw**ssxtrsxrwcxsiqtpkxirxlqx
sxtx*rsxcn*nr*xnh***tsx*ssxs*kxsxxn*fx*tixlkkikk*kikikxikkkk
*kkn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF382 (VFF382Q) /CSM/VF/VFF3-D/VFF382Q.Seq.d/                       1467   0.0
VFN722 (VFN722Q) /CSM/VF/VFN7-A/VFN722Q.Seq.d/                       1084   0.0
VFI626 (VFI626Q) /CSM/VF/VFI6-B/VFI626Q.Seq.d/                       1084   0.0
VFG581 (VFG581Q) /CSM/VF/VFG5-D/VFG581Q.Seq.d/                       1084   0.0
VFF663 (VFF663Q) /CSM/VF/VFF6-C/VFF663Q.Seq.d/                       1084   0.0
VFF530 (VFF530Q) /CSM/VF/VFF5-B/VFF530Q.Seq.d/                       1084   0.0
VFE361 (VFE361Q) /CSM/VF/VFE3-C/VFE361Q.Seq.d/                       1084   0.0
VFE318 (VFE318Q) /CSM/VF/VFE3-A/VFE318Q.Seq.d/                       1084   0.0
AFF396 (AFF396Q) /CSM/AF/AFF3-D/AFF396Q.Seq.d/                       1084   0.0
AFF388 (AFF388Q) /CSM/AF/AFF3-D/AFF388Q.Seq.d/                       1084   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BG140383|BG140383.1 EST480825 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP17G1 5' sequence, mRNA sequence.    92  1e-16  3
BG131085|BG131085.1 EST463977 tomato crown gall Lycopersicon esculentum cDNA clone cTOE2D11 5' sequence, mRNA sequence.    92  2e-16  3
AW217896|AW217896.1 EST296610 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD1C15, mRNA sequence.    92  2e-15  2
AI778006|AI778006.1 EST258885 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES3N17, mRNA sequence.    92  2e-15  2
AW217261|AW217261.1 EST295975 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC89G22, mRNA sequence.    92  2e-15  2
AW219042|AW219042.1 EST301524 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX2H3, mRNA sequence.    92  2e-15  2
BG138134|BG138134.1 EST478576 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP8K18 5' sequence, mRNA sequence.    92  3e-15  2
BG642411|BG642411.1 EST355887 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD11C15 5' end, mRNA sequence.    92  3e-15  2
BG601025|BG601025.1 EST505920 cSTS Solanum tuberosum cDNA clone cSTS30D8 5' sequence, mRNA sequence.    90  1e-14  2
BI935302|BI935302.1 EST555191 tomato flower, anthesis Lycopersicon esculentum cDNA clone cTOD22O12 5' end, mRNA sequence.    92  1e-14  1
dna update 2003. 9.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55BZ4) RecName: Full=Ethanolamine-phosphate cytidylyltransfera...   369   e-101
(Q5EA75) RecName: Full=Ethanolamine-phosphate cytidylyltransfera...   199   1e-49
AL663030_9(AL663030|pid:none) Mouse DNA sequence from clone RP23...   199   1e-49
AK166319_1(AK166319|pid:none) Mus musculus mammary gland RCB-052...   199   1e-49
AL663030_11(AL663030|pid:none) Mouse DNA sequence from clone RP2...   198   2e-49
BT077764_1(BT077764|pid:none) Lepeophtheirus salmonis clone lsal...   195   2e-48
BC088018_1(BC088018|pid:none) Xenopus tropicalis phosphate cytid...   195   2e-48
AY189524_1(AY189524|pid:none) Mus musculus CTP:ethanolaminephosp...   194   3e-48
AE014134_2379(AE014134|pid:none) Drosophila melanogaster chromos...   192   1e-47
AC087079_11(AC087079|pid:none) Caenorhabditis elegans cosmid Y37...   188   2e-46
protein update 2009. 6.19
PSORT 

 psg: 0.62  gvh: 0.53  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: mitochondrial
	 24.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFF382 is nuc
5' end seq. ID VFF382F
5' end seq. 
>VFF382F.Seq
AAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTAGAGTTTATGTTGATGGTT
GTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGACAAGCAAGAGAATTAGGAG
ATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAAAAAATAAAGGTCCACCAG
TTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTAAATGGGCAGATGAAGTTG
CAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATAGTTTAAACTGTGATTTTT
GTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAGATGTTTATGAAGGCATTA
AAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTGTTTCAACAACCGAGTTGG
TTGGTCGTATGTTATTATGCACCAAAGACCATCTTCAAAATGTTTCAGGTGAGCAAACCT
CACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAATCACCATACACAAGTTTAT
CACATTTNC----------
Length of 5' end seq. 549
3' end seq. ID VFF382Z
3' end seq. 
>VFF382Z.Seq
----------TCATGTCGTTANGTCGATGAAGTTGTCATTGGTGCTCCATTCAGTGTCNC
CAAAAATATGATTGACTCNCTTCNCATCAATGTTGTCGTTCATGGTGATGATCAAGTTGN
ACTCGGTCCANAAGGTGGTGTANATCCATACAAACTCCCAAAANAATTAGGNATTTACAA
ANAAGTTNAACANACTGAAGGTCNCNCTGCAACTGAAATCGTTAAAANAATCATTGATAA
TAGACTTCAANATGAAGCTCGNAATCGTAAAAAANAAGCNAAANAAATTAATTTCNTTGA
ACNATCNAATTAAAAAAAATTAAAAAATAAAAAATAAAAATAAAACNAATAAAAAAAAAA
AAATAGAAAAAAAATTT
Length of 3' end seq. 367
Connected seq. ID VFF382P
Connected seq. 
>VFF382P.Seq
AAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTAGAGTTTATGTTGATGGTT
GTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGACAAGCAAGAGAATTAGGAG
ATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAAAAAATAAAGGTCCACCAG
TTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTAAATGGGCAGATGAAGTTG
CAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATAGTTTAAACTGTGATTTTT
GTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAGATGTTTATGAAGGCATTA
AAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTGTTTCAACAACCGAGTTGG
TTGGTCGTATGTTATTATGCACCAAAGACCATCTTCAAAATGTTTCAGGTGAGCAAACCT
CACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAATCACCATACACAAGTTTAT
CACATTTNC----------TCATGTCGTTANGTCGATGAAGTTGTCATTGGTGCTCCATT
CAGTGTCNCCAAAAATATGATTGACTCNCTTCNCATCAATGTTGTCGTTCATGGTGATGA
TCAAGTTGNACTCGGTCCANAAGGTGGTGTANATCCATACAAACTCCCAAAANAATTAGG
NATTTACAAANAAGTTNAACANACTGAAGGTCNCNCTGCAACTGAAATCGTTAAAANAAT
CATTGATAATAGACTTCAANATGAAGCTCGNAATCGTAAAAAANAAGCNAAANAAATTAA
TTTCNTTGAACNATCNAATTAAAAAAAATTAAAAAATAAAAAATAAAAATAAAACNAATA
AAAAAAAAAAAATAGAAAAAAAATTT
Length of connected seq. 916
Full length Seq ID -
Full length Seq. -
Length of full length seq. -