>VFF269 (VFF269Q) /CSM/VF/VFF2-C/VFF269Q.Seq.d/
ACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGGAAAGCAATACTCTT
GGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAGTCGATGGTAAAG
TTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAGTTTTACTTTTAG
CAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGTCAGATAAACCAA
CATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCACATGAACATCCAAAATTTG
TTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAAACATTTTAAAGCAA
CCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGCATTGGTTTATCTTT
TAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTTTTATTTAAATTGTT
TCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCATATGATGCATGATT
CATGTCATGCTGCCATTTCACATTATCCAGGCGTATGGAAATGGATGGGCGCATCATTTG
ATTTCGTCACTGGTGCTTCATTCCTTXXXXXXXXXXTGTCACTCCATTCCAAACTCGTTC
ATGGTATCATAAATATCAACACATTTACGCTCCACTCCTCTATGGTATTTACACACTCAA
ATATCGTACTCAAGATTGGGAAGCTTTTGTAAAGGATGGTAAAAATGGTGCAATTCGTGT
TAGTGTCGCCACAAATTTCGATAAGGCCGCTTACGTCATTGGTAAATTGTCTTTTGTTTT
CTTCCGTTTCATCCTTCCACTCCGTTATCATAGCTTTACAGATTTAATTTGTTATTTCCT
CATTGCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGC
TGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGA
AGATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGCTCACTCCTTTG
TACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGC
TCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACAT
TACTTACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACACATTAATTACCTTTA
CAAAATGGGTAATGATCCAGATTATGTTAAAAAACCATAGCCTCAAAAGA

iy**NCNKMSKVITGKQYSWSELAKHNTENDCWVAVDGKVYDITRWVPLHPGGKEVLLLA
AGRDVTNLFESYHPMSDKPTSILKNYEIGYISSHEHPKFVQKSDFYKTLKERVRKHFKAT
DQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAIVYALCNSLFSMHMMHDS
CHAAISHYPGVWKWMGASFDFVTGASFL---

---VTPFQTRSWYHKYQHIYAPLLYGIYTLKYRTQDWEAFVKDGKNGAIRVSVATNFDKA
AYVIGKLSFVFFRFILPLRYHSFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATP
ERPDEPSQINEDWAILQLKTTQDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVP
IVKEVCKEHNITYHIKPNFTEAIMSHINYLYKMGNDPDYVKKP*pqk

Frame A:
tyinrivikcqk*lqesntlgqs*pnitlkmivglqsmvkfmilqdgyhyiqvvkkfyf*
qqvemlqiylkviiqcqinqhqf*rimkldifhhmniqnlfknqistrh*krelenilkq
pikihkwqlvfshv*hwfif*cl*lti*hiipvtifi*ivswqsfthfathysqci*cmi
hvmlpfhiiqaygngwahhlisslvlhs---

---chsipnsfmvs*isthlrstplwylhtqisysrlgsfckgw*kwcnsc*crhkfr*g
rlrhw*ivfcflpfhpstpls*lyrfnllfphc*irlwlvshn*fps*scr*rsqilcyp
*ktr*tisnq*rlgnpst*nysrlwswltplylf*wffkssscssfipincsrflpttct
nckrsl*rt*hylph*tklh*syyvth*lplqng**srlc*ktiask

Frame B:
hiliel**nvksnyrkaillvrvsqt*h*k*llgcsrw*sl*yykmgtitsrw*rsftfs
sr*rcykfi*klssnvr*tninfkel*nwiyfit*tskicskirflqdikres*ktf*sn
rsrstngs*yfltfsiglsfsvcnllfstlyq*qflfklflgnrlrtlqliilnayda*f
mscchftlsrrmemdgrii*frhwcfip---

---VTPFQTRSWYHKYQHIYAPLLYGIYTLKYRTQDWEAFVKDGKNGAIRVSVATNFDKA
AYVIGKLSFVFFRFILPLRYHSFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATP
ERPDEPSQINEDWAILQLKTTQDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVP
IVKEVCKEHNITYHIKPNFTEAIMSHINYLYKMGNDPDYVKKP*pqk

Frame C:
iy**NCNKMSKVITGKQYSWSELAKHNTENDCWVAVDGKVYDITRWVPLHPGGKEVLLLA
AGRDVTNLFESYHPMSDKPTSILKNYEIGYISSHEHPKFVQKSDFYKTLKERVRKHFKAT
DQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAIVYALCNSLFSMHMMHDS
CHAAISHYPGVWKWMGASFDFVTGASFL---

---slhsklvhgiinintftlhssmvfthsnivlkigkll*rmvkmvqfvlvspqisirp
ltslvncllfssvssfhsviialqi*fvissllnsslvgisqlisklvmslkisnsllpl
kdqmnhlksmkigqsfnlkllkimvmahsfvpflvvl*iiklfiiyshqllkisthnlyq
l*kkfvknitllttlnqtslkllchtlitftkwvmiqimlknhslkr

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF269 (VFF269Q) /CSM/VF/VFF2-C/VFF269Q.Seq.d/                       2537   0.0
VFB864 (VFB864Q) /CSM/VF/VFB8-C/VFB864Q.Seq.d/                       1328   0.0
VFI746 (VFI746Q) /CSM/VF/VFI7-B/VFI746Q.Seq.d/                       1314   0.0
VFE188 (VFE188Q) /CSM/VF/VFE1-D/VFE188Q.Seq.d/                       1314   0.0
SSB813 (SSB813Q) /CSM/SS/SSB8-A/SSB813Q.Seq.d/                       1314   0.0
SLA259 (SLA259Q) /CSM/SL/SLA2-C/SLA259Q.Seq.d/                       1314   0.0
SLA256 (SLA256Q) /CSM/SL/SLA2-C/SLA256Q.Seq.d/                       1314   0.0
VFM295 (VFM295Q) /CSM/VF/VFM2-D/VFM295Q.Seq.d/                       1312   0.0
VFF662 (VFF662Q) /CSM/VF/VFF6-C/VFF662Q.Seq.d/                       1312   0.0
VFN123 (VFN123Q) /CSM/VF/VFN1-A/VFN123Q.Seq.d/                       1310   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BD092935|BD092935.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants.  1304  0.0  2
BD082645|BD082645.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids.  1304  0.0  2
BD082630|BD082630.1 Methods and compositions for synthesis of long chain poly-unsaturated fatty acids.  1304  0.0  2
AB029311|AB029311.1 Dictyostelium discoideum Dd des5 gene for fatty acid desaturase, complete cds.   969  0.0  5
AB022097|AB022097.1 Dictyostelium discoideum mRNA for delta 5 fatty acid desaturase, complete cds.   149  e-115  12
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    34  0.16  16
AL355612|AL355612.8 Human DNA sequence from clone RP11-343P23 on chromosome 6 Contains  GSSs.    42  0.17  3
AC129112|AC129112.4 Rattus norvegicus clone CH230-265O9, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    48  0.28  1
AC137050|AC137050.1 Rattus norvegicus clone CH230-unknown, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    48  0.28  1
AC117308|AC117308.7 Rattus norvegicus clone CH230-216M16, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    48  0.28  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   467   0.0
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   323   e-157
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   267   e-139
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   162   1e-73
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   159   4e-73
CQ831428_1(CQ831428|pid:none) Sequence 9 from Patent WO2004057001.    143   1e-59
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   147   2e-59
CP000592_278(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   156   3e-57
CP001574_328(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   146   3e-57
AM949596_1(AM949596|pid:none) Mantoniella squamata d5 gene for A...   132   1e-56

 psg: 0.70  gvh: 0.24  alm: 0.25  top: 0.63  tms: 0.07  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.05  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VFF269 is cyt

>VFF269F.Seq
ACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGGAAAGCAATACTCTT
GGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAGTCGATGGTAAAG
TTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAGTTTTACTTTTAG
CAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGTCAGATAAACCAA
CATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCACATGAACATCCAAAATTTG
TTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAAACATTTTAAAGCAA
CCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGCATTGGTTTATCTTT
TAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTTTTATTTAAATTGTT
TCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCATATGATGCATGATT
CATGTCATGCTGCCATTTCACATTATCCAGGCGTATGGAAATGGATGGGCGCATCATTTG
ATTTCGTCACTGGTGCTTCATTCCTT----------

>VFF269Z.Seq
----------TGTCACTCCATTCCAAACTCGTTCATGGTATCATAAATATCAACACATTT
ACGCTCCACTCCTCTATGGTATTTACACACTCAAATATCGTACTCAAGATTGGGAAGCTT
TTGTAAAGGATGGTAAAAATGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGATAAGG
CCGCTTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTT
ATCATAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGGT
ATCTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACCC
CTGAAAGACCAGATGAACCATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTAAAA
CTACTCAAGATTATGGTCATGGCTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATC
ATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTAC
CAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTTACCACATTAAACCAAACTTCA
CTGAAGCTATTATGTCACACATTAATTACCTTTACAAAATGGGTAATGATCCAGATTATG
TTAAAAAACCATAGCCTCAAAAGA

>VFF269P.Seq
ACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGGAAAGCAATACTCTT
GGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAGTCGATGGTAAAG
TTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAGTTTTACTTTTAG
CAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGTCAGATAAACCAA
CATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCACATGAACATCCAAAATTTG
TTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAAACATTTTAAAGCAA
CCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGCATTGGTTTATCTTT
TAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTTTTATTTAAATTGTT
TCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCATATGATGCATGATT
CATGTCATGCTGCCATTTCACATTATCCAGGCGTATGGAAATGGATGGGCGCATCATTTG
ATTTCGTCACTGGTGCTTCATTCCTT----------TGTCACTCCATTCCAAACTCGTTC
ATGGTATCATAAATATCAACACATTTACGCTCCACTCCTCTATGGTATTTACACACTCAA
ATATCGTACTCAAGATTGGGAAGCTTTTGTAAAGGATGGTAAAAATGGTGCAATTCGTGT
TAGTGTCGCCACAAATTTCGATAAGGCCGCTTACGTCATTGGTAAATTGTCTTTTGTTTT
CTTCCGTTTCATCCTTCCACTCCGTTATCATAGCTTTACAGATTTAATTTGTTATTTCCT
CATTGCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGC
TGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGA
AGATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGCTCACTCCTTTG
TACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGC
TCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACAT
TACTTACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACACATTAATTACCTTTA
CAAAATGGGTAATGATCCAGATTATGTTAAAAAACCATAGCCTCAAAAGA