>VFF253 (VFF253Q) /CSM/VF/VFF2-C/VFF253Q.Seq.d/
ATTACAAGCATTGAAAAAAATGAACAAATCAATTTTATTATTAGTTTTAGTAATTGTATC
AATTATTGGTTCAATTGACTCAAAGAAAGTATTCAATGTTCGTACTGATTTAAAAAATCA
ATTAAAATTTAGACATTTTGATGATAGTTTAGATTATGATGCAATCAATTATCAATGGTT
CACTCAAAGTGTTGATCATTTTAATCCAGCAAACCCAACAACTTTCCAACAAAGATATTT
AATTAATGACCAATATTGGGATGGTACAGGTCCAGTTTTTATCATGATTAATGGTGAAGG
TCCAATGGATATTAATACTGTAACCCAACTTCAATTTGTTGTTTGGGCTAAACAAGTTAG
TGCACTTGTTGTTTCTCTTGAACATCGTTATTATGGTGCTAGTTTTGTAACTGAAGACCT
TTCACTTGAAAATTTACAATGGTTAAATTCAGCACAAGCATTAGCAGATAATGCAGTTTT
TAGAAATTTTGTAGCACAACAATATAATGTACCAAAAGAATCAAAATGGATTTCATTTGG
TGGTAXXXXXXXXXX

LQALKKMNKSILLLVLVIVSIIGSIDSKKVFNVRTDLKNQLKFRHFDDSLDYDAINYQWF
TQSVDHFNPANPTTFQQRYLINDQYWDGTGPVFIMINGEGPMDINTVTQLQFVVWAKQVS
ALVVSLEHRYYGASFVTEDLSLENLQWLNSAQALADNAVFRNFVAQQYNVPKESKWISFG
G---

Frame A:
itsiekneqinfiisfsncinywfn*lkesiqcsy*fkksiki*tf***frl*cnqlsmv
hskc*sf*sskpnnfptkifn**pilgwyrssfyhd*w*rsngy*ycnptsicclg*ts*
ctccfs*tsllwc*fcn*rpft*kftmvkfstsisr*csf*kfcstti*ctkrikmdfiw
w---


Frame B:
LQALKKMNKSILLLVLVIVSIIGSIDSKKVFNVRTDLKNQLKFRHFDDSLDYDAINYQWF
TQSVDHFNPANPTTFQQRYLINDQYWDGTGPVFIMINGEGPMDINTVTQLQFVVWAKQVS
ALVVSLEHRYYGASFVTEDLSLENLQWLNSAQALADNAVFRNFVAQQYNVPKESKWISFG
G---


Frame C:
ykh*kk*tnqfyy*f**lyqllvqltqrkysmfvli*kin*nldilmiv*immqsiings
lkvliiliqqtqqlsnkdi*lmtnigmvqvqfls*lmvkvqwilil*pnfnllfglnklv
hllfllnivimvlvl*lktfhlkiyng*iqhkh*qimqfleil*hnnimyqknqngfhlv
---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ823 (VSJ823Q) /CSM/VS/VSJ8-A/VSJ823Q.Seq.d/                       1041   0.0
VFO856 (VFO856Q) /CSM/VF/VFO8-C/VFO856Q.Seq.d/                       1041   0.0
VFO647 (VFO647Q) /CSM/VF/VFO6-B/VFO647Q.Seq.d/                       1041   0.0
VFO629 (VFO629Q) /CSM/VF/VFO6-B/VFO629Q.Seq.d/                       1041   0.0
VFN637 (VFN637Q) /CSM/VF/VFN6-B/VFN637Q.Seq.d/                       1041   0.0
VFN534 (VFN534Q) /CSM/VF/VFN5-B/VFN534Q.Seq.d/                       1041   0.0
VFN438 (VFN438Q) /CSM/VF/VFN4-B/VFN438Q.Seq.d/                       1041   0.0
VFN372 (VFN372Q) /CSM/VF/VFN3-C/VFN372Q.Seq.d/                       1041   0.0
VFN363 (VFN363Q) /CSM/VF/VFN3-C/VFN363Q.Seq.d/                       1041   0.0
VFN304 (VFN304Q) /CSM/VF/VFN3-A/VFN304Q.Seq.d/                       1041   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BI920191|BI920191.1 EST540126 potato microtubers, in vitro-grown Solanum tuberosum cDNA clone cSTE24M6 5' end, mRNA sequence.    44  9e-06  2
AF297520|AF297520.1 Haemophilus ducreyi alanyl-tRNA synthase (alaS), carbon storage regulator (csrA), and UDP-glucose pyrophosphorylase (galU) genes, complete cds.    40  0.034  2
BI178421|BI178421.1 EST519366 cSTE Solanum tuberosum cDNA clone cSTE13N22 5' sequence, mRNA sequence.    40  0.062  2
AC084578|AC084578.1 Caenorhabditis briggsae cosmid G39O07, complete sequence.    40  0.18  2
AL713960|AL713960.17 Mouse DNA sequence from clone RP23-448I9 on chromosome 11.    36  0.39  6
AK127029|AK127029.1 Homo sapiens cDNA FLJ45085 fis, clone BRAWH3028754.    36  0.44  3
AW705505|AW705505.1 sk49h12.y1 Gm-c1019 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1019-5952 5', mRNA sequence.    46  0.46  1
AI441512|AI441512.1 sa87c11.y1 Gm-c1004 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1004-6285 5', mRNA sequence.    46  0.46  1
AW705268|AW705268.1 sk59a02.y1 Gm-c1019 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1019-6819 5', mRNA sequence.    46  0.46  1
BI317694|BI317694.1 saf19h09.y1 Gm-c1076 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1076-1506 5', mRNA sequence.    46  0.46  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC133755_1(BC133755|pid:none) Xenopus laevis hypothetical LOC495...   118   9e-26
BC108759_1(BC108759|pid:none) Xenopus laevis hypothetical protei...   118   9e-26
BT080886_1(BT080886|pid:none) Caligus clemensi clone ccle-evs-50...   116   3e-25
BC161646_1(BC161646|pid:none) Xenopus tropicalis hypothetical pr...   112   5e-24
AE014296_1236(AE014296|pid:none) Drosophila melanogaster chromos...   107   2e-22
S44851(S44851)K12H4.7 protein - Caenorhabditis elegans                102   7e-21
L14331_6(L14331|pid:none) Caenorhabditis elegans cosmid K12H4, c...   102   7e-21
(P34528) RecName: Full=Putative serine protease K12H4.7;        ...   102   7e-21
T16490(T16490)hypothetical protein F56F10.1 - Caenorhabditis ele...   100   4e-20
(P90893) RecName: Full=Putative serine protease F56F10.1;       ...   100   4e-20

 psg: 0.99  gvh: 0.72  alm: 0.37  top: 0.40  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: endoplasmic reticulum
	  8.0 %: cytoplasmic
	  8.0 %: vacuolar
	  8.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for VFF253 is exc

>VFF253F.Seq
ATTACAAGCATTGAAAAAAATGAACAAATCAATTTTATTATTAGTTTTAGTAATTGTATC
AATTATTGGTTCAATTGACTCAAAGAAAGTATTCAATGTTCGTACTGATTTAAAAAATCA
ATTAAAATTTAGACATTTTGATGATAGTTTAGATTATGATGCAATCAATTATCAATGGTT
CACTCAAAGTGTTGATCATTTTAATCCAGCAAACCCAACAACTTTCCAACAAAGATATTT
AATTAATGACCAATATTGGGATGGTACAGGTCCAGTTTTTATCATGATTAATGGTGAAGG
TCCAATGGATATTAATACTGTAACCCAACTTCAATTTGTTGTTTGGGCTAAACAAGTTAG
TGCACTTGTTGTTTCTCTTGAACATCGTTATTATGGTGCTAGTTTTGTAACTGAAGACCT
TTCACTTGAAAATTTACAATGGTTAAATTCAGCACAAGCATTAGCAGATAATGCAGTTTT
TAGAAATTTTGTAGCACAACAATATAATGTACCAAAAGAATCAAAATGGATTTCATTTGG
TGGTA----------