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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFF242
Library VF
(Link to library)
Clone ID VFF242
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16605-1
Original site URL
Representative seq. ID VFF242P
(Link to Original site)
Representative DNA sequence 
>VFF242 (VFF242Q) /CSM/VF/VFF2-B/VFF242Q.Seq.d/
AATTAAATATATATATACTAAAAATGAGAGTTTTATCATTCCTTTGTTTATTATTAGTTA
GCTACGCTTCTGCTAAACAACAATTCTCTGAATTACAATACAGAAATGCTTTCACCAACT
GGATGCAAGCTCACCAAAGAACTTATTCCTCTGAAGAATTTAATGCTCGTTATCAAATCT
TCAAATCCAATATGGATTATGTACACCAATGGAATTCAAAAGGTGGTGAAACCGTTTTGG
GTTTAAATGTTTTCGCTGATATTACCAACCAAGAATATAGAACTACCTACTTGGGTACCC
CATTCGATGGTTCAGCCCTCATTGGTACTGAAGAAGAGAAAATCTTCTCCACCCCAGCCC
CAACTGTTGATTGGAGAGCTCAAGGTGCTGTCACACCAATTAAAAATCAAGGTCAATGTG
GTGGCTGCTGGTCATTCTCAACCACTGGTTCAACTGAAGGTGCTCACTTTATTGCATCTG
GAACAAAAAAAGATTTAGTTTCATTATCTGAACAAAACTTGATCGATTGTTCAAAATCAT
ACGGTAACAATGGTTGTGAAGGTGGTTTAATGACTCTTGCCTTTGAATATATCATCAATA
ACAAAGGTATTGATACTGAAAXXXXXXXXXXGGTTCTGAAGCTTCATTACAATCAGCATC
AAACAATGCTCCAGTCTCTGTTGCAATTGATGCTTCAAATGAATCCTTCCAATTATATGA
ATCAGGTATCTACTATGAACCAGCATGTTCTCCAACCCAACTTGATCATGGTGTTTTAGT
TGTTGGTTATGGTTCAGGTTCAAGTTCATCATCTGGTTCATCATCTGGTAAATCATCATC
ATCATCATCAACTGGTGGTAAAACTTCATCCTCATCATCATCAGGTAAAGCTTCATCATC
ATCATCAGGCAAAGCTTCATCATCATCATCATCAGGTAAAACTTCATCTGCTGCTTCATC
AACCTCTGGTTCTCAATCAGGTTCCCAATCAGGTAGCCAATCAGGCCAATCCACCGGTTC
ACAATCAGGTCAAACCTCTGCTTCGTCAAGCATCAGCATCAGGTTCTGGTTCTGGCTCAG
GTTCAGGTTCAGGTTCAGGTTCAGGCTCAGGTGCTGTTGAGGCCTCATCTGGTAACTACT
GGATCGTTAAAAACTCATGGGGTACTTCATGGGGTATGGATGGTTACATTTTTATGAGCA
AAGATAGAAATAACAATTGTGGTATCGCAACAATGGCTTCTTTCCCAACTGCCTCATCAA
ATTAAAATTTTATTTTTAATTGTCCGACTAACATTAATTTATTGTAATAAATAAAAAAAA
TAAAAATAAAAAATAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LNIYILKMRVLSFLCLLLVSYASAKQQFSELQYRNAFTNWMQAHQRTYSSEEFNARYQIF
KSNMDYVHQWNSKGGETVLGLNVFADITNQEYRTTYLGTPFDGSALIGTEEEKIFSTPAP
TVDWRAQGAVTPIKNQGQCGGCWSFSTTGSTEGAHFIASGTKKDLVSLSEQNLIDCSKSY
GNNGCEGGLMTLAFEYIINNKGIDTE---

---GSEASLQSASNNAPVSVAIDASNESFQLYESGIYYEPACSPTQLDHGVLVVGYGSGS
SSSSGSSSGKSSSSSSTGGKTSSSSSSGKASSSSSGKASSSSSSGKTSSAASSTSGSQSG
SQSGSQSGQSTGSQSGQTSASSSISIRFWFWLRFRFRFRFRLRCC*gliw*lldr*klmg
yfmgygwlhfyeqr*k*qlwyrnngffpncliklkfyf*lsd*h*fivinkknknkk*
Translated Amino Acid sequence (All Frames) 
Frame A:
n*iyiy*k*efyhsfvyy*latlllnnnslnyntemlsptgckltkeliplknlmlviks
snpiwimytngiqkvvkpfwv*mfslilptknielptwvphsmvqpslvlkkrkssppqp
qlligelkvlshqlkikvnvvaaghsqplvqlkvltllhleqkki*fhylnkt*sivqnh
tvtmvvkvv**llplnissitkvlilk---

---GSEASLQSASNNAPVSVAIDASNESFQLYESGIYYEPACSPTQLDHGVLVVGYGSGS
SSSSGSSSGKSSSSSSTGGKTSSSSSSGKASSSSSGKASSSSSSGKTSSAASSTSGSQSG
SQSGSQSGQSTGSQSGQTSASSSISIRFWFWLRFRFRFRFRLRCC*gliw*lldr*klmg
yfmgygwlhfyeqr*k*qlwyrnngffpncliklkfyf*lsd*h*fivinkknknkk*

Frame B:
ikyiytknesfiiplfiis*lrfc*ttil*itiqkcfhqldasspknlfl*ri*cslsnl
qiqyglctpmefkrw*nrfgfkcfr*yyqpri*nyllgypirwfsphwy*rrenllhpsp
nc*lessrcchtn*ksrsmwwllvilnhwfn*rcslyciwnkkrfsfii*tkldrlfkii
r*qwl*rwfndscl*iyhq*qry*y*---

---vlklhynqhqtmlqsllqlmlqmnpsnymnqvstmnqhvlqpnlimvf*llvmvqvq
vhhlvhhlvnhhhhhqlvvklhphhhqvklhhhhqaklhhhhhqvklhlllhqplvlnqv
pnqvanqanppvhnqvkpllrqasasgsgsgsgsgsgsgsgsgaveassgnywivknswg
tswgmdgyifmskdrnnncgiatmasfptassn*nfifncptninll**ikkikiknk

Frame C:
LNIYILKMRVLSFLCLLLVSYASAKQQFSELQYRNAFTNWMQAHQRTYSSEEFNARYQIF
KSNMDYVHQWNSKGGETVLGLNVFADITNQEYRTTYLGTPFDGSALIGTEEEKIFSTPAP
TVDWRAQGAVTPIKNQGQCGGCWSFSTTGSTEGAHFIASGTKKDLVSLSEQNLIDCSKSY
GNNGCEGGLMTLAFEYIINNKGIDTE---

---f*sfitisikqcsslccn*cfk*ilpii*iryll*tsmfsnpt*swcfscwlwfrfk
fiiwfiiw*iiiiiinww*nfiliiir*sfiiiirqsfiiiiir*nficcfinlwfsirf
pir*pirpihrftirsnlcfvkhqhqvlvlaqvqvqvqvqaqvllrphlvttgslkthgv
lhgvwmvtfl*akieitivvsqqwllsqlphqikilflivrltliycnk*kk*k*ki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF242 (VFF242Q) /CSM/VF/VFF2-B/VFF242Q.Seq.d/                       1518   0.0
VFN628 (VFN628Q) /CSM/VF/VFN6-B/VFN628Q.Seq.d/                       1189   0.0
VFN294 (VFN294Q) /CSM/VF/VFN2-D/VFN294Q.Seq.d/                       1189   0.0
VFM804 (VFM804Q) /CSM/VF/VFM8-A/VFM804Q.Seq.d/                       1189   0.0
VFL463 (VFL463Q) /CSM/VF/VFL4-C/VFL463Q.Seq.d/                       1189   0.0
VFL389 (VFL389Q) /CSM/VF/VFL3-D/VFL389Q.Seq.d/                       1189   0.0
VFK680 (VFK680Q) /CSM/VF/VFK6-D/VFK680Q.Seq.d/                       1189   0.0
VFK619 (VFK619Q) /CSM/VF/VFK6-A/VFK619Q.Seq.d/                       1189   0.0
VFK133 (VFK133Q) /CSM/VF/VFK1-B/VFK133Q.Seq.d/                       1189   0.0
VFI606 (VFI606Q) /CSM/VF/VFI6-A/VFI606Q.Seq.d/                       1189   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

L36204|L36204.1 Dictyostelium discoideum cysteine proteinase (CP4) mRNA, complete cds.   922  0.0  7
L36205|L36205.1 Dictyostelium discoideum cysteine proteinase CP5 mRNA, complete cds.   121  e-146  10
U72746|U72746.1 Dictyostelium discoideum cysteine proteinase (cprG) mRNA, complete cds.   145  e-144  8
AC117072|AC117072.2 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.   121  e-126  13
U72745|U72745.1 Dictyostelium discoideum cysteine proteinase (cprF) mRNA, complete cds.   115  e-117  7
X03344|X03344.1 Dictyostelium discoideum mRNA for cysteine proteinase 2.    76  9e-66  8
M16039|M16039.1 Dictyostelium discoideum pst-cath gene encoding pst-cathepsin, complete cds.    74  2e-52  10
X02407|X02407.1 D.discoideum mRNA for cysteine proteinase 1.      70  2e-18  3
BJ171907|BJ171907.1 Physcomitrella patens subsp. patens cDNA clone:pph30i20, 3' end, single read.    66  1e-11  2
AJ489298|AJ489298.1 Aphis gossypii mRNA for putative cathepsin L (catL gene).    56  3e-10  3
dna update 2003. 9.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

L36204_1(L36204|pid:none) Dictyostelium discoideum cysteine prot...   410   e-138
(Q94504) RecName: Full=Cysteine proteinase 7;          EC=3.4.22...   327   e-104
(P54640) RecName: Full=Cysteine proteinase 5;          EC=3.4.22...   313   e-101
L36205_1(L36205|pid:none) Dictyostelium discoideum cysteine prot...   306   1e-99
(Q94503) RecName: Full=Cysteine proteinase 6;          EC=3.4.22...   315   2e-99
(P04989) RecName: Full=Cysteine proteinase 2;          EC=3.4.22...   230   3e-78
EF053509_1(EF053509|pid:none) Acanthamoeba castellanii cysteine ...   203   2e-64
AC117076_20(AC117076|pid:none) Dictyostelium discoideum chromoso...   172   2e-55
AY220615_1(AY220615|pid:none) Hydra vulgaris cathepsin L precurs...   164   3e-54
EU143709_1(EU143709|pid:none) Stichopus japonicus cathepsin L mR...   157   4e-52
protein update 2009. 6.19
PSORT 

 psg: 0.96  gvh: 0.87  alm: 0.51  top: 0.40  tms: 0.00  mit: 0.37  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: extracellular, including cell wall
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoplasmic
	  4.0 %: Golgi

>> prediction for VFF242 is exc
5' end seq. ID VFF242F
5' end seq. 
>VFF242F.Seq
AATTAAATATATATATACTAAAAATGAGAGTTTTATCATTCCTTTGTTTATTATTAGTTA
GCTACGCTTCTGCTAAACAACAATTCTCTGAATTACAATACAGAAATGCTTTCACCAACT
GGATGCAAGCTCACCAAAGAACTTATTCCTCTGAAGAATTTAATGCTCGTTATCAAATCT
TCAAATCCAATATGGATTATGTACACCAATGGAATTCAAAAGGTGGTGAAACCGTTTTGG
GTTTAAATGTTTTCGCTGATATTACCAACCAAGAATATAGAACTACCTACTTGGGTACCC
CATTCGATGGTTCAGCCCTCATTGGTACTGAAGAAGAGAAAATCTTCTCCACCCCAGCCC
CAACTGTTGATTGGAGAGCTCAAGGTGCTGTCACACCAATTAAAAATCAAGGTCAATGTG
GTGGCTGCTGGTCATTCTCAACCACTGGTTCAACTGAAGGTGCTCACTTTATTGCATCTG
GAACAAAAAAAGATTTAGTTTCATTATCTGAACAAAACTTGATCGATTGTTCAAAATCAT
ACGGTAACAATGGTTGTGAAGGTGGTTTAATGACTCTTGCCTTTGAATATATCATCAATA
ACAAAGGTATTGATACTGAAA----------
Length of 5' end seq. 621
3' end seq. ID VFF242Z
3' end seq. 
>VFF242Z.Seq
----------GGTTCTGAAGCTTCATTACAATCAGCATCAAACAATGCTCCAGTCTCTGT
TGCAATTGATGCTTCAAATGAATCCTTCCAATTATATGAATCAGGTATCTACTATGAACC
AGCATGTTCTCCAACCCAACTTGATCATGGTGTTTTAGTTGTTGGTTATGGTTCAGGTTC
AAGTTCATCATCTGGTTCATCATCTGGTAAATCATCATCATCATCATCAACTGGTGGTAA
AACTTCATCCTCATCATCATCAGGTAAAGCTTCATCATCATCATCAGGCAAAGCTTCATC
ATCATCATCATCAGGTAAAACTTCATCTGCTGCTTCATCAACCTCTGGTTCTCAATCAGG
TTCCCAATCAGGTAGCCAATCAGGCCAATCCACCGGTTCACAATCAGGTCAAACCTCTGC
TTCGTCAAGCATCAGCATCAGGTTCTGGTTCTGGCTCAGGTTCAGGTTCAGGTTCAGGTT
CAGGCTCAGGTGCTGTTGAGGCCTCATCTGGTAACTACTGGATCGTTAAAAACTCATGGG
GTACTTCATGGGGTATGGATGGTTACATTTTTATGAGCAAAGATAGAAATAACAATTGTG
GTATCGCAACAATGGCTTCTTTCCCAACTGCCTCATCAAATTAAAATTTTATTTTTAATT
GTCCGACTAACATTAATTTATTGTAATAAATAAAAAAAATAAAAATAAAAAATAAA
Length of 3' end seq. 706
Connected seq. ID VFF242P
Connected seq. 
>VFF242P.Seq
AATTAAATATATATATACTAAAAATGAGAGTTTTATCATTCCTTTGTTTATTATTAGTTA
GCTACGCTTCTGCTAAACAACAATTCTCTGAATTACAATACAGAAATGCTTTCACCAACT
GGATGCAAGCTCACCAAAGAACTTATTCCTCTGAAGAATTTAATGCTCGTTATCAAATCT
TCAAATCCAATATGGATTATGTACACCAATGGAATTCAAAAGGTGGTGAAACCGTTTTGG
GTTTAAATGTTTTCGCTGATATTACCAACCAAGAATATAGAACTACCTACTTGGGTACCC
CATTCGATGGTTCAGCCCTCATTGGTACTGAAGAAGAGAAAATCTTCTCCACCCCAGCCC
CAACTGTTGATTGGAGAGCTCAAGGTGCTGTCACACCAATTAAAAATCAAGGTCAATGTG
GTGGCTGCTGGTCATTCTCAACCACTGGTTCAACTGAAGGTGCTCACTTTATTGCATCTG
GAACAAAAAAAGATTTAGTTTCATTATCTGAACAAAACTTGATCGATTGTTCAAAATCAT
ACGGTAACAATGGTTGTGAAGGTGGTTTAATGACTCTTGCCTTTGAATATATCATCAATA
ACAAAGGTATTGATACTGAAA----------GGTTCTGAAGCTTCATTACAATCAGCATC
AAACAATGCTCCAGTCTCTGTTGCAATTGATGCTTCAAATGAATCCTTCCAATTATATGA
ATCAGGTATCTACTATGAACCAGCATGTTCTCCAACCCAACTTGATCATGGTGTTTTAGT
TGTTGGTTATGGTTCAGGTTCAAGTTCATCATCTGGTTCATCATCTGGTAAATCATCATC
ATCATCATCAACTGGTGGTAAAACTTCATCCTCATCATCATCAGGTAAAGCTTCATCATC
ATCATCAGGCAAAGCTTCATCATCATCATCATCAGGTAAAACTTCATCTGCTGCTTCATC
AACCTCTGGTTCTCAATCAGGTTCCCAATCAGGTAGCCAATCAGGCCAATCCACCGGTTC
ACAATCAGGTCAAACCTCTGCTTCGTCAAGCATCAGCATCAGGTTCTGGTTCTGGCTCAG
GTTCAGGTTCAGGTTCAGGTTCAGGCTCAGGTGCTGTTGAGGCCTCATCTGGTAACTACT
GGATCGTTAAAAACTCATGGGGTACTTCATGGGGTATGGATGGTTACATTTTTATGAGCA
AAGATAGAAATAACAATTGTGGTATCGCAACAATGGCTTCTTTCCCAACTGCCTCATCAA
ATTAAAATTTTATTTTTAATTGTCCGACTAACATTAATTTATTGTAATAAATAAAAAAAA
TAAAAATAAAAAATAAA
Length of connected seq. 1327
Full length Seq ID -
Full length Seq. -
Length of full length seq. -