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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM452
Library AF
(Link to library)
Clone ID AFM452
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM452P
(Link to Original site)
Representative DNA sequence 
>AFM452 (AFM452Q) /CSM/AF/AFM4-C/AFM452Q.Seq.d/
AATAAACAATCAAATAACACAAATACATATAATTTAAATTCAAAAAAAATAATAGAATTA
AATACAGTAATTTTTTTTGTATTTTTATATCTATCATTCATACTTTAAAAAAAAAAXXXX
XXXXXXTANAACTACTGGTATACAAGAATCAGAATTTACATTTGATAAGATTAGATTAAA
GATTGTAGATGTCGGTGGTCAAANATNTCAAANAANAAAATGGATTCATTGTTTCGATTG
TGTTACAGCAGTTATTTTNGTTGCCGCTATGAGCGATTACGATCAAGNACTTANAGAAGA
TGAATCTGTAAATAGAACTANAGAATCCTTAGCCTTATTCAAANAGATTGTAAATTGTGA
TTACTTTAAANAGACNCCNATCGTGTTATTCCTCAACAANAAGGATCTTTTCAAGGAAAA
ATTAAANAGAGNACCNCNCCAATCTTGGTTCTCTGATTNCNCTGGNCCAAATAAANATAA
ANATGCTGCCATGTTCATTCAATCNCAATATCTTGCNCAAGGTCCTTC
sequence update 2009. 4. 3
Translated Amino Acid sequence 
*tik*hkyi*FKFKKNNRIKYSNFFCIFISIIHTLKKK---

---XTTGIQESEFTFDKIRLKIVDVGGQXXQXXKWIHCFDCVTAVIXVAAMSDYDQXLXE
DESVNRTXESLALFKXIVNCDYFKXTPIVLFLNXKDLFKEKLXRXPXQSWFSDXXGPNKX
KXAAMFIQSQYLAQGP
Translated Amino Acid sequence (All Frames) 
Frame A:
nkqsnntntynlnskkiielntviffvflylsfil*kk---

---xnywytririyi**d*ikdcrcrwsxxsxxkmdslfrlcyssyfxcryerlrsxtxr
r*ick*nxrilsliqxdckl*ll*xdxxrvipqqxgsfqgkikxxtxpilvl*xxwxk*x
*xcchvhsixiscxrsf

Frame B:
innqitqihii*iqkk**n*iq*fflyfyiyhsyfkkk---

---XTTGIQESEFTFDKIRLKIVDVGGQXXQXXKWIHCFDCVTAVIXVAAMSDYDQXLXE
DESVNRTXESLALFKXIVNCDYFKXTPIVLFLNXKDLFKEKLXRXPXQSWFSDXXGPNKX
KXAAMFIQSQYLAQGP

Frame C:
*tik*hkyi*FKFKKNNRIKYSNFFCIFISIIHTLKKK---

---xllvyknqnlhlirld*rl*msvvkxxkxxngfivsivlqqlfxlpl*aitikxlxk
mnl*ielxnp*pysxrl*ivitlxrxxscysstxrifsrkn*xexxxnlgslixlxqixi
xmlpcsfnxnilxkvl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM452 (AFM452Q) /CSM/AF/AFM4-C/AFM452Q.Seq.d/                        613   e-174
SFK575 (SFK575Q) /CSM/SF/SFK5-D/SFK575Q.Seq.d/                        605   e-172
CHA330 (CHA330Q) /CSM/CH/CHA3-B/CHA330Q.Seq.d/                        605   e-172
SLA332 (SLA332Q) /CSM/SL/SLA3-B/SLA332Q.Seq.d/                        496   e-139
CHA430 (CHA430Q) /CSM/CH/CHA4-B/CHA430Q.Seq.d/                        371   e-102
AFI437 (AFI437Q) /CSM/AF/AFI4-B/AFI437Q.Seq.d/                        272   5e-72
CFI616 (CFI616Q) /CSM/CF/CFI6-A/CFI616Q.Seq.d/                        260   2e-68
SLC719 (SLC719Q) /CSM/SL/SLC7-A/SLC719Q.Seq.d/                        258   8e-68
AFO563 (AFO563Q) /CSM/AF/AFO5-C/AFO563Q.Seq.d/                        252   5e-66
AFG338 (AFG338Q) /CSM/AF/AFG3-B/AFG338Q.Seq.d/                        204   1e-51
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ346930) Dictyostelium discoideum cDNA clone:dda25h14, 3' ...   648   0.0    1
(S55498) G alpha 4=Guanine nucleotide-binding protein [Dicty...   640   e-179  1
(BJ401295) Dictyostelium discoideum cDNA clone:dds22e19, 3' ...   640   e-179  1
(BJ377044) Dictyostelium discoideum cDNA clone:ddc22l07, 3' ...   640   e-179  1
(AU040057) Dictyostelium discoideum slug cDNA, clone SLA332.      402   e-145  2
(BJ377045) Dictyostelium discoideum cDNA clone:ddc22l08, 3' ...   406   e-109  1
(AU034341) Dictyostelium discoideum slug cDNA, clone SLC719.      293   3e-75  1
(BJ343843) Dictyostelium discoideum cDNA clone:dda17j10, 3' ...   272   1e-68  1
(BJ375298) Dictyostelium discoideum cDNA clone:ddc18o04, 3' ...   260   5e-65  1
(BJ346359) Dictyostelium discoideum cDNA clone:dda30m15, 3' ...   252   1e-62  1
dna update 2009. 4.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34042) RecName: Full=Guanine nucleotide-binding protein alpha-...   223   2e-57
AB274883_1(AB274883|pid:none) Mauremys reevesii mRNA for guanine...   130   1e-29
AK056008_1(AK056008|pid:none) Homo sapiens cDNA FLJ31446 fis, cl...   130   2e-29
AY369812_1(AY369812|pid:none) Pan troglodytes guanine nucleotide...   130   2e-29
(P09471) RecName: Full=Guanine nucleotide-binding protein G(o) s...   130   2e-29
AY369813_1(AY369813|pid:none) Macaca mulatta guanine nucleotide-...   130   2e-29
BC059637_1(BC059637|pid:none) Danio rerio guanine nucleotide bin...   130   2e-29
BC030027_1(BC030027|pid:none) Homo sapiens guanine nucleotide bi...   130   2e-29
(P34044) RecName: Full=Guanine nucleotide-binding protein alpha-...   130   3e-29
M19182_1(M19182|pid:none) Homo sapiens guanine nucleotide-bindin...   130   3e-29
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM452 is

5' end seq. ID AFM452F
5' end seq. 
>AFM452F.Seq
AATAAACAATCAAATAACACAAATACATATAATTTAAATTCAAAAAAAATAATAGAATTA
AATACAGTAATTTTTTTTGTATTTTTATATCTATCATTCATACTTTAAAAAAAAAAXXXX
XXXXXX
Length of 5' end seq. 126
3' end seq. ID AFM452Z
3' end seq. 
>AFM452Z.Seq
XXXXXXXXXXTANAACTACTGGTATACAAGAATCAGAATTTACATTTGATAAGATTAGAT
TAAAGATTGTAGATGTCGGTGGTCAAANATNTCAAANAANAAAATGGATTCATTGTTTCG
ATTGTGTTACAGCAGTTATTTTNGTTGCCGCTATGAGCGATTACGATCAAGNACTTANAG
AAGATGAATCTGTAAATAGAACTANAGAATCCTTAGCCTTATTCAAANAGATTGTAAATT
GTGATTACTTTAAANAGACNCCNATCGTGTTATTCCTCAACAANAAGGATCTTTTCAAGG
AAAAATTAAANAGAGNACCNCNCCAATCTTGGTTCTCTGATTNCNCTGGNCCAAATAAAN
ATAAANATGCTGCCATGTTCATTCAATCNCAATATCTTGCNCAAGGTCCTTC
Length of 3' end seq. 412
Connected seq. ID AFM452P
Connected seq. 
>AFM452P.Seq
AATAAACAATCAAATAACACAAATACATATAATTTAAATTCAAAAAAAATAATAGAATTA
AATACAGTAATTTTTTTTGTATTTTTATATCTATCATTCATACTTTAAAAAAAAAA----
------TANAACTACTGGTATACAAGAATCAGAATTTACATTTGATAAGATTAGATTAAA
GATTGTAGATGTCGGTGGTCAAANATNTCAAANAANAAAATGGATTCATTGTTTCGATTG
TGTTACAGCAGTTATTTTNGTTGCCGCTATGAGCGATTACGATCAAGNACTTANAGAAGA
TGAATCTGTAAATAGAACTANAGAATCCTTAGCCTTATTCAAANAGATTGTAAATTGTGA
TTACTTTAAANAGACNCCNATCGTGTTATTCCTCAACAANAAGGATCTTTTCAAGGAAAA
ATTAAANAGAGNACCNCNCCAATCTTGGTTCTCTGATTNCNCTGGNCCAAATAAANATAA
ANATGCTGCCATGTTCATTCAATCNCAATATCTTGCNCAAGGTCCTTC
Length of connected seq. 518
Full length Seq ID -
Full length Seq. -
Length of full length seq. -