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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFF227
Library VF
(Link to library)
Clone ID VFF227
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16602-1
Original site URL
Representative seq. ID VFF227E
(Link to Original site)
Representative DNA sequence 
>VFF227 (VFF227Q) /CSM/VF/VFF2-B/VFF227Q.Seq.d/
AGTCTCAGTATTACTTTATCTTTAAATTCTCATACACAACAAGATGTCCGGGGCAGGAAG
TAAAAGAAAGAACGTTTTCATTGAAAAAGCTACCAAGCTTTTCACCACTTACGACAAAAT
GATCGTCGCCGAAGCTGATTTTGTAGGTTCAAGCCAATTACAAAAGATCAGAAAATCCAT
CCGTGGTATCGGTGCCGTTCTCATGGGTAAGAAGACTATGATCCGTAAGGTCATCCGTGA
TTTAGCTGATTCTAAACCAGAACTCGATGCCTTAAATACCTACTTAAAACAAAACACCTG
TATCATTTTCTGTAAAGATAACATCGCTGAAGTTAAACGTGTTATCAATACTCAACGTGT
TGGTGCCCCAGCTAAAGCAGGTGTTTTCGCTCCAAACGATGTTATCATCCCAGCCGGTCC
AACTGGTATGGAACCAACCCAAACCTCTTTCCTCCAAGATTTAAAGATTGCCACTAAAAT
CAACAGAGGTCAAATCGATATCGTCAATGAAGTCCACATCATTAAAACCGGTCAAAAAGT
TGGTGCCTCAGAAGCTACCCTNTTACAAAAATTGAACATCAAACCATTCACCTATGGTTT
AGAACCAAAAATCATCTATGATGCTGGTGCCTGTTATTCACCATCTATCTCTGAAGAAGA
TCTCATCAACAAATTCAAACAAGGTATCTTCAACATTGCTGCCATCTCCTTGGAAATTGG
TTACCCAACCGTTGCTTCAATTCCACATTCAGTCATGAATGCTTTCAAGAATTTATTAGC
CATCTCATTCGAAACCTCTTACACTTTCGATGCTGCTGAAAAATTCAAATCAGCCGCTGC
TGCTGCCCCAGTTGCTGCCGCTCCATCCGCTGCTGCTCCAGCTGCCGCTGCCAAGAAAGT
CGTCGTCGAAGAAAAGAAAGAAGAATCAGATGATGATATGGGTATGGGTCTCTTNGATTA
AGATTNAATAAATAAATTTTTTTTTTCAAAAA
sequence update 2001. 6. 9
Translated Amino Acid sequence 
vsvllyl*ILIHNKMSGAGSKRKNVFIEKATKLFTTYDKMIVAEADFVGSSQLQKIRKSI
RGIGAVLMGKKTMIRKVIRDLADSKPELDALNTYLKQNTCIIFCKDNIAEVKRVINTQRV
GAPAKAGVFAPNDVIIPAGPTGMEPTQTSFLQDLKIATKINRGQIDIVNEVHIIKTGQKV
GASEATLLQKLNIKPFTYGLEPKIIYDAGACYSPSISEEDLINKFKQGIFNIAAISLEIG
YPTVASIPHSVMNAFKNLLAISFETSYTFDAAEKFKSAAAAAPVAAAPSAAAPAAAAKKV
VVEEKKEESDDDMGMGLXD*dxinkfffsk
Translated Amino Acid sequence (All Frames) 
Frame A:
slsitlslnshtqqdvrgrk*kkerfh*ksyqafhhlrqndrrrs*fcrfkpitkdqkih
pwyrcrshg*edydp*ghp*fs*f*trtrclkyllktkhlyhfl*r*hr*s*tcyqystc
wcps*srcfrskrcyhpsrsnwygtnpnlfpprfkdch*nqqrsnryrq*sphh*nrsks
wclrsypxtkiehqtihlwfrtknhl*cwcllftiyl*rrshqqiqtrylqhcchllgnw
lpnrcfnstfshecfqefishlirnllhfrcc*kiqisrcccpsccrsirccsscrcqes
rrrrkerrir**ygygslxlrxnk*ifffk


Frame B:
vsvllyl*ILIHNKMSGAGSKRKNVFIEKATKLFTTYDKMIVAEADFVGSSQLQKIRKSI
RGIGAVLMGKKTMIRKVIRDLADSKPELDALNTYLKQNTCIIFCKDNIAEVKRVINTQRV
GAPAKAGVFAPNDVIIPAGPTGMEPTQTSFLQDLKIATKINRGQIDIVNEVHIIKTGQKV
GASEATLLQKLNIKPFTYGLEPKIIYDAGACYSPSISEEDLINKFKQGIFNIAAISLEIG
YPTVASIPHSVMNAFKNLLAISFETSYTFDAAEKFKSAAAAAPVAAAPSAAAPAAAAKKV
VVEEKKEESDDDMGMGLXD*dxinkfffsk


Frame C:
sqyyfifkfsyttrcpgqevkertfslkklpsfsplttk*sspklil*vqanykrsenps
vvsvpfswvrrl*svrssvi*lilnqnsmp*ipt*nktpvsfsvkitslklnvlsilnvl
vpqlkqvfslqtmlssqpvqlvwnqpkplsski*rlplkstevksissmkstslkpvkkl
vpqklpxykn*tsnhspmv*nqkssmmlvpvihhlslkkisstnsnkvsstllpspwklv
tqpllqfhiqs*mlsriy*pshskpltlsmllknsnqplllpqllplhplllqlplprks
sskkrkknqmmiwvwvsxikix*infffqk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL888 (VFL888Q) /CSM/VF/VFL8-D/VFL888Q.Seq.d/                       1643   0.0
VFG702 (VFG702Q) /CSM/VF/VFG7-A/VFG702Q.Seq.d/                       1643   0.0
VFF854 (VFF854Q) /CSM/VF/VFF8-C/VFF854Q.Seq.d/                       1643   0.0
VFF227 (VFF227Q) /CSM/VF/VFF2-B/VFF227Q.Seq.d/                       1643   0.0
VFE158 (VFE158Q) /CSM/VF/VFE1-C/VFE158Q.Seq.d/                       1643   0.0
VFD767 (VFD767Q) /CSM/VF/VFD7-C/VFD767Q.Seq.d/                       1643   0.0
VFB326 (VFB326Q) /CSM/VF/VFB3-B/VFB326Q.Seq.d/                       1643   0.0
VFA871 (VFA871Q) /CSM/VF/VFA8-C/VFA871Q.Seq.d/                       1643   0.0
VFA341 (VFA341Q) /CSM/VF/VFA3-B/VFA341Q.Seq.d/                       1643   0.0
VFF848 (VFF848Q) /CSM/VF/VFF8-B/VFF848Q.Seq.d/                       1641   0.0
own update 2003.10.30
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X56194|X56194.1 D. discoideum mRNA for ribosomal acidic phosphoprotein P0.  1544  0.0  2
CD203589|CD203589.1 Ls_AM1_10H07_T7 Litomosoides sigmodontis adult male 1 Litomosoides sigmodontis cDNA clone Ls_AM1_10H07 5', mRNA sequence.    62  7e-07  2
AA901452|AA901452.1 SWOv3MCAM03C04 Onchocerca volvulus molting L3 larva cDNA (SL96MLW-OvmL3) Onchocerca volvulus cDNA clone onch28 5' similar to TR:P91913 P91913 RIBOSOMAL PROTEIN P1 HOMOLOG. ;contains element MER22 repetitive element ;, mRNA sequence.    54  3e-04  2
BG310507|BG310507.1 SWOv3MCAM56B08SK Onchocerca volvulus molting L3 larva cDNA (SL96MLW-OvmL3) Onchocerca volvulus cDNA clone SWOv3MCAM56B08 5', mRNA sequence.    54  3e-04  2
AW355195|AW355195.1 PFOvAFCB597K Onchocerca volvulus adult female cDNA following ivermectin (SAW98PF-OvAF) Onchocerca volvulus cDNA clone PFOvAFCB597 5', mRNA sequence.    54  3e-04  2
BE760989|BE760989.1 SWOvL2CAS15E12SK Onchocerca volvulus L2 larvae cDNA (SAW98MLW-OvL2) Onchocerca volvulus cDNA clone SWOvL2CAS15E12 5', mRNA sequence.    54  3e-04  2
AI130065|AI130065.1 SWOvL3CAN53C10SK Onchocerca volvulus infective larva cDNA (SAW94WL-OvL3) Onchocerca volvulus cDNA clone SWOvL3CAN53C10 5', mRNA sequence.    54  3e-04  2
AA618720|AA618720.1 SWOv3MCA1033SK Onchocerca volvulus molting L3 larva cDNA (SL96MLW-OvmL3) Onchocerca volvulus cDNA clone SWmL3CO1033 5', mRNA sequence.    54  3e-04  2
BE491921|BE491921.1 SWOvMfCAR14E06SK Onchocerca volvulus microfilaria cDNA (SAW98MLW-OvMf) Onchocerca volvulus cDNA clone SWOvMfCAR14E06 5', mRNA sequence.    54  3e-04  2
AW288169|AW288169.1 SWOv3MCAM34F10SK Onchocerca volvulus molting L3 larva cDNA (SL96MLW-OvmL3) Onchocerca volvulus cDNA clone SWOv3MCAM34F10 5', mRNA sequence.    52  0.001  2
dna update 2003. 9.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P22685) RecName: Full=60S acidic ribosomal protein P0;               500   e-140
R5DOP0(S14012) ribosomal protein P0 - slime mold (Dictyostelium ...   496   e-139
AY857422_1(AY857422|pid:none) Suberites domuncula L10e/P0 mRNA, ...   252   2e-65
EU069460_1(EU069460|pid:none) Stylophora pistillata 60S acidic r...   244   3e-63
AY217739_1(AY217739|pid:none) Toxoplasma gondii ribosomal P prot...   244   4e-63
(Q9U3U0) RecName: Full=60S acidic ribosomal protein P0;         ...   242   2e-62
AF390866_1(AF390866|pid:none) Toxoplasma gondii ribosomal phosph...   241   4e-62
AB284186_1(AB284186|pid:none) Neospora caninum mRNA for ribosoma...   240   5e-62
AJ457827_1(AJ457827|pid:none) Bombyx mori mRNA for ribosomal P0 ...   239   1e-61
DQ516397_1(DQ516397|pid:none) Strongyloides ratti acidic ribosom...   239   1e-61
protein update 2009. 7.10
PSORT 

 psg: 0.62  gvh: 0.44  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for VFF227 is cyt
5' end seq. ID VFF227F
5' end seq. 
>VFF227F.Seq
AGTCTCAGTATTACTTTATCTTTAAATTCTCATACACAACAAGATGTCCGGGGCAGGAAG
TAAAAGAAAGAACGTTTTCATTGAAAAAGCTACCAAGCTTTTCACCACTTACGACAAAAT
GATCGTCGCCGAAGCTGATTTTGTAGGTTCAAGCCAATTACAAAAGATCAGAAAATCCAT
CCGTGGTATCGGTGCCGTTCTCATGGGTAAGAAGACTATGATCCGTAAGGTCATCCGTGA
TTTAGCTGATTCTAAACCAGAACTCGATGCCTTAAATACCTACTTAAAACAAAACACCTG
TATCATTTTCTGTAAAGATAACATCGCTGAAGTTAAACGTGTTATCAATACTCAACGTGT
TGGTGCCCCAGCTAAAGCAGGTGTTTTCGCTCCAAACGATGTTATCATCCCAGCCGGTCC
AACTGGTATGGAACCAACCCAAACCTCTTTCCTCCAAGATTTAAAGATTGCCACTAAAAT
CAACAGAGGTCAAATCGATATCGTCAATGAAGTCCACATCATTAAAACC----------
Length of 5' end seq. 529
3' end seq. ID VFF227Z
3' end seq. 
>VFF227Z.Seq
----------ACTGGTATGGAACCAACCCAAACCTCTTTCCTCCAAGATTTAAAGATTGC
CACTAAAATCAACAGAGGTCAAATCGATATCGTCAATGAAGTCCACATCATTAAAACCGG
TCAAAAAGTTGGTGCCTCAGAAGCTACCCTNTTACAAAAATTGAACATCAAACCATTCAC
CTATGGTTTAGAACCAAAAATCATCTATGATGCTGGTGCCTGTTATTCACCATCTATCTC
TGAAGAAGATCTCATCAACAAATTCAAACAAGGTATCTTCAACATTGCTGCCATCTCCTT
GGAAATTGGTTACCCAACCGTTGCTTCAATTCCACATTCAGTCATGAATGCTTTCAAGAA
TTTATTAGCCATCTCATTCGAAACCTCTTACACTTTCGATGCTGCTGAAAAATTCAAATC
AGCCGCTGCTGCTGCCCCAGTTGCTGCCGCTCCATCCGCTGCTGCTCCAGCTGCCGCTGC
CAAGAAAGTCGTCGTCGAAGAAAAGAAAGAAGAATCAGATGATGATATGGGTATGGGTCT
CTTNGATTAAGATTNAATAAATAAATTTTTTTTTTCAAAAA
Length of 3' end seq. 571
Connected seq. ID VFF227P
Connected seq. 
>VFF227P.Seq
AGTCTCAGTATTACTTTATCTTTAAATTCTCATACACAACAAGATGTCCGGGGCAGGAAG
TAAAAGAAAGAACGTTTTCATTGAAAAAGCTACCAAGCTTTTCACCACTTACGACAAAAT
GATCGTCGCCGAAGCTGATTTTGTAGGTTCAAGCCAATTACAAAAGATCAGAAAATCCAT
CCGTGGTATCGGTGCCGTTCTCATGGGTAAGAAGACTATGATCCGTAAGGTCATCCGTGA
TTTAGCTGATTCTAAACCAGAACTCGATGCCTTAAATACCTACTTAAAACAAAACACCTG
TATCATTTTCTGTAAAGATAACATCGCTGAAGTTAAACGTGTTATCAATACTCAACGTGT
TGGTGCCCCAGCTAAAGCAGGTGTTTTCGCTCCAAACGATGTTATCATCCCAGCCGGTCC
AACTGGTATGGAACCAACCCAAACCTCTTTCCTCCAAGATTTAAAGATTGCCACTAAAAT
CAACAGAGGTCAAATCGATATCGTCAATGAAGTCCACATCATTAAAACC----------A
CTGGTATGGAACCAACCCAAACCTCTTTCCTCCAAGATTTAAAGATTGCCACTAAAATCA
ACAGAGGTCAAATCGATATCGTCAATGAAGTCCACATCATTAAAACCGGTCAAAAAGTTG
GTGCCTCAGAAGCTACCCTNTTACAAAAATTGAACATCAAACCATTCACCTATGGTTTAG
AACCAAAAATCATCTATGATGCTGGTGCCTGTTATTCACCATCTATCTCTGAAGAAGATC
TCATCAACAAATTCAAACAAGGTATCTTCAACATTGCTGCCATCTCCTTGGAAATTGGTT
ACCCAACCGTTGCTTCAATTCCACATTCAGTCATGAATGCTTTCAAGAATTTATTAGCCA
TCTCATTCGAAACCTCTTACACTTTCGATGCTGCTGAAAAATTCAAATCAGCCGCTGCTG
CTGCCCCAGTTGCTGCCGCTCCATCCGCTGCTGCTCCAGCTGCCGCTGCCAAGAAAGTCG
TCGTCGAAGAAAAGAAAGAAGAATCAGATGATGATATGGGTATGGGTCTCTTNGATTAAG
ATTNAATAAATAAATTTTTTTTTTCAAAAA
Length of connected seq. 1100
Full length Seq ID VFF227E
Full length Seq. 
>VFF227E.Seq
AGTCTCAGTATTACTTTATCTTTAAATTCTCATACACAACAAGATGTCCGGGGCAGGAAG
TAAAAGAAAGAACGTTTTCATTGAAAAAGCTACCAAGCTTTTCACCACTTACGACAAAAT
GATCGTCGCCGAAGCTGATTTTGTAGGTTCAAGCCAATTACAAAAGATCAGAAAATCCAT
CCGTGGTATCGGTGCCGTTCTCATGGGTAAGAAGACTATGATCCGTAAGGTCATCCGTGA
TTTAGCTGATTCTAAACCAGAACTCGATGCCTTAAATACCTACTTAAAACAAAACACCTG
TATCATTTTCTGTAAAGATAACATCGCTGAAGTTAAACGTGTTATCAATACTCAACGTGT
TGGTGCCCCAGCTAAAGCAGGTGTTTTCGCTCCAAACGATGTTATCATCCCAGCCGGTCC
AACTGGTATGGAACCAACCCAAACCTCTTTCCTCCAAGATTTAAAGATTGCCACTAAAAT
CAACAGAGGTCAAATCGATATCGTCAATGAAGTCCACATCATTAAAACCGGTCAAAAAGT
TGGTGCCTCAGAAGCTACCCTNTTACAAAAATTGAACATCAAACCATTCACCTATGGTTT
AGAACCAAAAATCATCTATGATGCTGGTGCCTGTTATTCACCATCTATCTCTGAAGAAGA
TCTCATCAACAAATTCAAACAAGGTATCTTCAACATTGCTGCCATCTCCTTGGAAATTGG
TTACCCAACCGTTGCTTCAATTCCACATTCAGTCATGAATGCTTTCAAGAATTTATTAGC
CATCTCATTCGAAACCTCTTACACTTTCGATGCTGCTGAAAAATTCAAATCAGCCGCTGC
TGCTGCCCCAGTTGCTGCCGCTCCATCCGCTGCTGCTCCAGCTGCCGCTGCCAAGAAAGT
CGTCGTCGAAGAAAAGAAAGAAGAATCAGATGATGATATGGGTATGGGTCTCTTNGATTA
AGATTNAATAAATAAATTTTTTTTTTCAAAAA
Length of full length seq. 992