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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFF225
Library VF
(Link to library)
Clone ID VFF225
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15602-1
Original site URL
Representative seq. ID VFF225P
(Link to Original site)
Representative DNA sequence 
>VFF225 (VFF225Q) /CSM/VF/VFF2-B/VFF225Q.Seq.d/
CTGTTTTACAAGTCCATGGAGTTAATATCACTACAACCTTCTATAGAAATATGTTTAAAG
CTAATCCACAACTTTTAAATATTTTCAATCATTCAAATCAAAGAGAAGGAAAACAACAAA
ATGCTTTAGCAAATACAGTTTTACAAGCTGCCATTCATATTGATAAATTGAATGAATTAA
ATTTAGCTCCAATCGTTCATAAACATGTTGCATTGGGTGTATTACCAGAACATTATCCAA
TTGTTGGTACAAATTTATTGGGTGCAATTAAAGAAGTTCTTCAAGATGCTGCCACTGATG
AGATTTTAGGTGCATGGGGTGAAGCTTATGGTGTAATTGCTCAAGCTTTCATTGATGCTG
AAGCCGCTTTATATAAAGTTACAGAGGAGCAAATTGGAGGTTGGAGAGATACTCGTGAAT
TCATTGTCGATAGAAAGGTTGAAGAGTCGTCCAATATCATTTCATTCTACTTTAAACCAG
CCGATGGTAXXXXXXXXXXGAACTCACATCAGACATTACAGTTTATCAGACACACCATCC
GAACAATACTATCGTATTAGTGTAAAGAAAGAAGACGCCCTCAAAAAGTCTGATCCAAAT
GGTGTAGTTTCAAATCATTTGCATGCAAATGTTAAGGTTGGCGATAAAGTTTTACTCTCC
CCTCCAGCTGGTGACTATGTAGTCGACCAATTATCAAGCAATCCAATCCTTTTGGTTAGT
GGTGGTGTTGGTATCACTCCATTATTAAGTATGGCAAAGGCAACTTTAGCTAAACAACCA
GAAAGAGAAGTCACCTTTGTTCACTCTTCAAAGAACAAACAATATCAACCATTTGCAAAT
GAATTATCCCAATTGGAAAAATCAAATAAAGTAAAGGTATCAACTGTTCATTCTGAAACT
GATGGTCAAATCACCAAAGATAAATTAGAAAAATTCATCAATCCTTCTCAAATTAAAGAT
ACTAAAGTTTTCATCTGTGGTCCAGTATCTTTTATGTCTGCAATTAATAAACAATTAATT
GAATTAGGTTATCCAAAAGAAAATATTTCTTATGAAATTTTTGGTCCATTAACAAATGTA
TAAAAAAAAATAAAAAATAAAAAATAAAAAAAATAACATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
VLQVHGVNITTTFYRNMFKANPQLLNIFNHSNQREGKQQNALANTVLQAAIHIDKLNELN
LAPIVHKHVALGVLPEHYPIVGTNLLGAIKEVLQDAATDEILGAWGEAYGVIAQAFIDAE
AALYKVTEEQIGGWRDTREFIVDRKVEESSNIISFYFKPADG---

---THIRHYSLSDTPSEQYYRISVKKEDALKKSDPNGVVSNHLHANVKVGDKVLLSPPAG
DYVVDQLSSNPILLVSGGVGITPLLSMAKATLAKQPEREVTFVHSSKNKQYQPFANELSQ
LEKSNKVKVSTVHSETDGQITKDKLEKFINPSQIKDTKVFICGPVSFMSAINKQLIELGY
PKENISYEIFGPLTNV*kkiknkk*kk*h
Translated Amino Acid sequence (All Frames) 
Frame A:
lfyksmelislqpsieiclklihnf*ifsiiqikekennkml*qiqfyklpfilin*mn*
i*lqsfinmlhwvyyqniiqllvqiywvqlkkffkmlplmrf*vhgvklmv*llklslml
kplyiklqrsklevgeilvnslsierlksrpisfhstlnqpmv---

---eltsditvyqthhpnntivlv*rkktpsksliqmv*fqiicmqmlrlaikfysplql
vtm*stnyqaiqsfwlvvvlvslhy*vwqrql*lnnqkeksplftlqrtnninhlqmnyp
nwknqik*ryqlfilklmvkspkin*knssillklkilkfssvvqyllclqlinn*ln*v
iqkkiflmkflvh*qmykkk*kiknkknni

Frame B:
cftspws*yhynll*kyv*s*sttfkyfqsfkskrrkttkcfskysftschsy**ie*ik
fssnrs*tccigcitrtlsncwykfigcn*rsssrcch**dfrcmg*slwcncssfh*c*
srfi*syrganwrlerys*ihcr*kg*rvvqyhfill*tsrw---

---nshqtlqfirhtirtilsy*ckerrrpqkv*skwcsfksfackc*gwr*sftlpssw
*lcsrpiikqsnpfg*wwcwyhsiikygkgnfs*ttrkrshlcslfkeqtistick*iip
igkik*skgincsf*n*wsnhqr*irkihqsfsn*ry*sfhlwssifyvcn**tin*irl
skrkyfl*nfwsinkcikknkk*kikkit

Frame C:
VLQVHGVNITTTFYRNMFKANPQLLNIFNHSNQREGKQQNALANTVLQAAIHIDKLNELN
LAPIVHKHVALGVLPEHYPIVGTNLLGAIKEVLQDAATDEILGAWGEAYGVIAQAFIDAE
AALYKVTEEQIGGWRDTREFIVDRKVEESSNIISFYFKPADG---

---THIRHYSLSDTPSEQYYRISVKKEDALKKSDPNGVVSNHLHANVKVGDKVLLSPPAG
DYVVDQLSSNPILLVSGGVGITPLLSMAKATLAKQPEREVTFVHSSKNKQYQPFANELSQ
LEKSNKVKVSTVHSETDGQITKDKLEKFINPSQIKDTKVFICGPVSFMSAINKQLIELGY
PKENISYEIFGPLTNV*kkiknkk*kk*h
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF225 (VFF225Q) /CSM/VF/VFF2-B/VFF225Q.Seq.d/                       2083   0.0
VSH228 (VSH228Q) /CSM/VS/VSH2-B/VSH228Q.Seq.d/                       1154   0.0
VSA370 (VSA370Q) /CSM/VS/VSA3-C/VSA370Q.Seq.d/                       1154   0.0
SLA171 (SLA171Q) /CSM/SL/SLA1-C/SLA171Q.Seq.d/                       1154   0.0
VSA808 (VSA808Q) /CSM/VS/VSA8-A/VSA808Q.Seq.d/                       1148   0.0
SLC715 (SLC715Q) /CSM/SL/SLC7-A/SLC715Q.Seq.d/                       1142   0.0
VSD490 (VSD490Q) /CSM/VS/VSD4-D/VSD490Q.Seq.d/                       1120   0.0
VSI227 (VSI227Q) /CSM/VS/VSI2-B/VSI227Q.Seq.d/                       1094   0.0
FCL-AA17 (FCL-AA17Q) /CSM/FCL/FCL-AA/FCL-AA17Q.Seq.d/                1082   0.0
VFN493 (VFN493Q) /CSM/VF/VFN4-D/VFN493Q.Seq.d/                       1074   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AB025583|AB025583.1 Dictyostelium discoideum DdFHa mRNA for flavohemoglobin, complete cds.  1154  0.0  2
AB025584|AB025584.1 Dictyostelium discoideum DdFHb mRNA for flavohemoglobin, complete cds.    80  8e-51  8
AE017002|AE017002.1 Bacillus cereus ATCC 14579 section 5 of 18 of the complete genome.    46  0.004  9
AC097819|AC097819.6 Rattus norvegicus clone CH230-62A5, WORKING DRAFT SEQUENCE, 1 ordered piece.    42  0.36  5
CC258722|CC258722.1 CH261-79H18_Sp6.1 CH261 Gallus gallus genomic clone CH261-79H18, DNA sequence.    32  0.48  3
AE017028|AE017028.1 Bacillus anthracis str. Ames section 5 of 18 of the complete genome.    42  0.60  6
AC102259|AC102259.3 Mus musculus clone RP24-199C12, WORKING DRAFT SEQUENCE, 3 unordered pieces.    46  0.94  1
AZ718695|AZ718695.1 RPCI-24-159K19.TJ RPCI-24 Mus musculus genomic clone RPCI-24-159K19 , DNA sequence.    46  0.94  1
AC101138|AC101138.1 Mus musculus clone RP23-170L15, LOW-PASS SEQUENCE SAMPLING.    46  0.94  1
AB028630|AB028630.1 Clostridium perfringens hyp27, bacH, ptp, cpd genes for hypothetical protein, bacterial hemoglobin, protein-tyrosine phosphatase,  2', 3'-cuclic nucleotide 2'-phosphodiesterase, partial and complete cds.    46  0.94  1
dna update 2003. 9.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB025584_1(AB025584|pid:none) Dictyostelium discoideum DdFHb mRN...   226   e-116
AP008955_1144(AP008955|pid:none) Brevibacillus brevis NBRC 10059...   196   4e-85
CP000903_1350(CP000903|pid:none) Bacillus weihenstephanensis KBA...   179   5e-82
CP001186_1337(CP001186|pid:none) Bacillus cereus G9842, complete...   177   2e-81
(Q81FW4) RecName: Full=Flavohemoprotein; AltName: Full=Hemoglobi...   177   4e-81
CP001176_1375(CP001176|pid:none) Bacillus cereus B4264, complete...   177   9e-81
(Q73B49) RecName: Full=Flavohemoprotein; AltName: Full=Hemoglobi...   177   5e-80
CP001407_1354(CP001407|pid:none) Bacillus cereus 03BB102, comple...   177   8e-80
(Q6HLA6) RecName: Full=Flavohemoprotein; AltName: Full=Hemoglobi...   177   8e-80
CP000485_1227(CP000485|pid:none) Bacillus thuringiensis str. Al ...   177   1e-79
protein update 2009. 6.19
PSORT 

 psg: 0.85  gvh: 0.30  alm: 0.32  top: 0.73  tms: 0.07  mit: 0.36  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.08  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 52.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for VFF225 is nuc
5' end seq. ID VFF225F
5' end seq. 
>VFF225F.Seq
CTGTTTTACAAGTCCATGGAGTTAATATCACTACAACCTTCTATAGAAATATGTTTAAAG
CTAATCCACAACTTTTAAATATTTTCAATCATTCAAATCAAAGAGAAGGAAAACAACAAA
ATGCTTTAGCAAATACAGTTTTACAAGCTGCCATTCATATTGATAAATTGAATGAATTAA
ATTTAGCTCCAATCGTTCATAAACATGTTGCATTGGGTGTATTACCAGAACATTATCCAA
TTGTTGGTACAAATTTATTGGGTGCAATTAAAGAAGTTCTTCAAGATGCTGCCACTGATG
AGATTTTAGGTGCATGGGGTGAAGCTTATGGTGTAATTGCTCAAGCTTTCATTGATGCTG
AAGCCGCTTTATATAAAGTTACAGAGGAGCAAATTGGAGGTTGGAGAGATACTCGTGAAT
TCATTGTCGATAGAAAGGTTGAAGAGTCGTCCAATATCATTTCATTCTACTTTAAACCAG
CCGATGGTA----------
Length of 5' end seq. 489
3' end seq. ID VFF225Z
3' end seq. 
>VFF225Z.Seq
----------GAACTCACATCAGACATTACAGTTTATCAGACACACCATCCGAACAATAC
TATCGTATTAGTGTAAAGAAAGAAGACGCCCTCAAAAAGTCTGATCCAAATGGTGTAGTT
TCAAATCATTTGCATGCAAATGTTAAGGTTGGCGATAAAGTTTTACTCTCCCCTCCAGCT
GGTGACTATGTAGTCGACCAATTATCAAGCAATCCAATCCTTTTGGTTAGTGGTGGTGTT
GGTATCACTCCATTATTAAGTATGGCAAAGGCAACTTTAGCTAAACAACCAGAAAGAGAA
GTCACCTTTGTTCACTCTTCAAAGAACAAACAATATCAACCATTTGCAAATGAATTATCC
CAATTGGAAAAATCAAATAAAGTAAAGGTATCAACTGTTCATTCTGAAACTGATGGTCAA
ATCACCAAAGATAAATTAGAAAAATTCATCAATCCTTCTCAAATTAAAGATACTAAAGTT
TTCATCTGTGGTCCAGTATCTTTTATGTCTGCAATTAATAAACAATTAATTGAATTAGGT
TATCCAAAAGAAAATATTTCTTATGAAATTTTTGGTCCATTAACAAATGTATAAAAAAAA
ATAAAAAATAAAAAATAAAAAAAATAACATA
Length of 3' end seq. 621
Connected seq. ID VFF225P
Connected seq. 
>VFF225P.Seq
CTGTTTTACAAGTCCATGGAGTTAATATCACTACAACCTTCTATAGAAATATGTTTAAAG
CTAATCCACAACTTTTAAATATTTTCAATCATTCAAATCAAAGAGAAGGAAAACAACAAA
ATGCTTTAGCAAATACAGTTTTACAAGCTGCCATTCATATTGATAAATTGAATGAATTAA
ATTTAGCTCCAATCGTTCATAAACATGTTGCATTGGGTGTATTACCAGAACATTATCCAA
TTGTTGGTACAAATTTATTGGGTGCAATTAAAGAAGTTCTTCAAGATGCTGCCACTGATG
AGATTTTAGGTGCATGGGGTGAAGCTTATGGTGTAATTGCTCAAGCTTTCATTGATGCTG
AAGCCGCTTTATATAAAGTTACAGAGGAGCAAATTGGAGGTTGGAGAGATACTCGTGAAT
TCATTGTCGATAGAAAGGTTGAAGAGTCGTCCAATATCATTTCATTCTACTTTAAACCAG
CCGATGGTA----------GAACTCACATCAGACATTACAGTTTATCAGACACACCATCC
GAACAATACTATCGTATTAGTGTAAAGAAAGAAGACGCCCTCAAAAAGTCTGATCCAAAT
GGTGTAGTTTCAAATCATTTGCATGCAAATGTTAAGGTTGGCGATAAAGTTTTACTCTCC
CCTCCAGCTGGTGACTATGTAGTCGACCAATTATCAAGCAATCCAATCCTTTTGGTTAGT
GGTGGTGTTGGTATCACTCCATTATTAAGTATGGCAAAGGCAACTTTAGCTAAACAACCA
GAAAGAGAAGTCACCTTTGTTCACTCTTCAAAGAACAAACAATATCAACCATTTGCAAAT
GAATTATCCCAATTGGAAAAATCAAATAAAGTAAAGGTATCAACTGTTCATTCTGAAACT
GATGGTCAAATCACCAAAGATAAATTAGAAAAATTCATCAATCCTTCTCAAATTAAAGAT
ACTAAAGTTTTCATCTGTGGTCCAGTATCTTTTATGTCTGCAATTAATAAACAATTAATT
GAATTAGGTTATCCAAAAGAAAATATTTCTTATGAAATTTTTGGTCCATTAACAAATGTA
TAAAAAAAAATAAAAAATAAAAAATAAAAAAAATAACATA
Length of connected seq. 1110
Full length Seq ID -
Full length Seq. -
Length of full length seq. -