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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFF224
Library VF
(Link to library)
Clone ID VFF224
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15434-1
Original site URL
Representative seq. ID VFF224P
(Link to Original site)
Representative DNA sequence 
>VFF224 (VFF224Q) /CSM/VF/VFF2-A/VFF224Q.Seq.d/
TTCAGCGTTTAAACATACACACTTAAGAGAATGACTAAATTACACTACAAAGTTAAAGAT
ATTTCACTTGCCGCTTGGGGTCGTAAGGAAATTGAAATTGCCGAAAATGAAATGCCAGGT
TTAATGACCTTAAGAAAGAAATATGGTCCAGCTCAAATCTTAAAAGGTGCTCGTATTGCA
GGTTGTTTACACATGACTATCCAAACCGCCGTTTTAATTGAAACTTTAACTGCTCTCGGT
GCTCAAGTCCAATGGTCATCATGTAACATTTTCTCCACTCAAGATCAAGCCGCCGCTGCC
ATCGCTGCCACTGGTGTCCCAGTCTATGCCTGGAAAGGTGAAACCGAAGAAGAATACAAC
TGGTGTGTCGAACAAACCATTGTTTTCCAAGATGGTCAACCATTAAATATGATCTTAGAT
GATGGTGGTGATTTAACCAACCTCGTCCACGAGAAATACCCACAATTCTTAGCTGGTATC
AAAGGTATCTCTGAAGAAACCACCACTGGTGTCCACAACCTCTACAAAATGTTCAAAGAA
GGTAAATTAAAGGTCCCAGCCATCAACGTCAATGACTCTGTCACCAAATCCAAATTCGAT
AACTTATATGGTTGTCGTGAATCTTTAATCGATGGTATTAAACGTGCCAXXXXXXXXXXA
TCTTTAATCGATGGTATTAAACGTGCCACCGATGTTATGATTGCCGGTAAAGTTGCCGTC
GTCGCTGGTTACGGTGATGTAGGTAAAGGTTGTGCTCAATCATTATCAAAAATGGGTGCT
CGTGTTTTAGTCACTGAAATCGATCCAATCAATGCCCTCCAAGCCTGTATGGATGGTTAC
CAAATCGTCACCATGGAAACCGCCGCTCCATTATCAAACATTTTCGTCACCACCACCGGT
TGTCGTGATATCGTCAGAGGTGAACACTTTGCCGTCATGAAAGAAGATGCCATCGTTTGT
AACATTGGTCACTTTGATTGTGAAATCGATGTCGCTTGGTTAAACGCCAACGCCAAAAAA
GATACCGTCAAACCACAAGTTGACCGTTACACCCTTGCCAACGGTGTCCACATCATCCTC
TTAGCTGAAGGTCGTCTCGTCAATTTAGGTTGTGGTACTGGTCATCCATCTTTTGTTATG
TCAAACTCTTTCTGTAACCAGACTTTAGCTCAAATCGCCCTCTGGACTAAAACTGAAGAA
TACCCATTAGGTGTCCACTTCTTACCAAAGATTTTAGATGAAGAAGTTGCTCGTTTACAT
TTAGATCAATTAGGTGCTAAATTAACTACCCTCACTGAAAAACAATCCGAATATTTATCA
GTTCCAGTCGCTGGTCCATACAAAGTNATCACTACAGAATTAAAATCTTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
fsv*TYTLKRMTKLHYKVKDISLAAWGRKEIEIAENEMPGLMTLRKKYGPAQILKGARIA
GCLHMTIQTAVLIETLTALGAQVQWSSCNIFSTQDQAAAAIAATGVPVYAWKGETEEEYN
WCVEQTIVFQDGQPLNMILDDGGDLTNLVHEKYPQFLAGIKGISEETTTGVHNLYKMFKE
GKLKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRA---

---SLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQSLSKMGARVLVTEIDPINALQACM
DGYQIVTMETAAPLSNIFVTTTGCRDIVRGEHFAVMKEDAIVCNIGHFDCEIDVAWLNAN
AKKDTVKPQVDRYTLANGVHIILLAEGRLVNLGCGTGHPSFVMSNSFCNQTLAQIALWTK
TEEYPLGVHFLPKILDEEVARLHLDQLGAKLTTLTEKQSEYLSVPVAGPYKVITTELKS
Translated Amino Acid sequence (All Frames) 
Frame A:
fsv*TYTLKRMTKLHYKVKDISLAAWGRKEIEIAENEMPGLMTLRKKYGPAQILKGARIA
GCLHMTIQTAVLIETLTALGAQVQWSSCNIFSTQDQAAAAIAATGVPVYAWKGETEEEYN
WCVEQTIVFQDGQPLNMILDDGGDLTNLVHEKYPQFLAGIKGISEETTTGVHNLYKMFKE
GKLKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRA---

---ifnrwy*tchrcydcr*scrrrwlr*cr*rlcsiiikngcscfsh*nrsnqcppsly
gwlpnrhhgnrrsiikhfrhhhrls*yrqr*tlcrherrchrl*hwsl*l*nrcrlvkrq
rqkryrqtts*plhpcqrcphhpls*rssrqfrlwywssifcyvklfl*pdfssnrpld*
n*ripircplltkdfr*rscsftfrsirc*inyph*ktirifisssrwsiqsxhyrikif

Frame B:
safkhthlre*lnyttklkifhlplgvvrklklpkmkcqv**p*ernmvqlks*kvlvlq
vvyt*lskppf*lkl*llsvlksnghhvtfsplkikpplpslplvsqsmpgkvkpkkntt
gvsnkplfskmvnh*i*s*mmvvi*ptsstrnthns*lvskvslkkpplvsttstkcskk
vn*rsqpstsmtlspnpnsitymvvvnl*smvlnvp---

---SLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQSLSKMGARVLVTEIDPINALQACM
DGYQIVTMETAAPLSNIFVTTTGCRDIVRGEHFAVMKEDAIVCNIGHFDCEIDVAWLNAN
AKKDTVKPQVDRYTLANGVHIILLAEGRLVNLGCGTGHPSFVMSNSFCNQTLAQIALWTK
TEEYPLGVHFLPKILDEEVARLHLDQLGAKLTTLTEKQSEYLSVPVAGPYKVITTELKS

Frame C:
qrlniht*end*itlqs*ryftcrlgs*gn*ncrk*narfndlkkeiwsssnlkrcsycr
lfthdypnrrfn*nfncsrcsspmvim*hflhsrssrrchrchwcpslcler*nrrriql
vcrtnhcfprwstikydlr*ww*fnqprpreiptilswyqryl*rnhhwcpqplqnvqrr
*ikgpshqrq*lchqiqir*liwls*ifnrwy*tc---

---l*smvlnvppml*lpvklpsslvtvm*vkvvlnhyqkwvlvf*slksiqsmpskpvw
mvtksspwkpplhyqtfsspppvvvissevntlps*kkmpsfvtlvtlivksmslg*tpt
pkkipsnhkltvtplptvstsss*lkvvssi*vvvlvihlllcqtlsvtrl*lkspsglk
lknth*vstsyqrf*mkkllvyi*in*vln*lpslknnpniyqfqslvhtkxslqn*nl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF224 (VFF224Q) /CSM/VF/VFF2-A/VFF224Q.Seq.d/                       2650   0.0
CHB217 (CHB217Q) /CSM/CH/CHB2-A/CHB217Q.Seq.d/                       1396   0.0
VFI402 (VFI402Q) /CSM/VF/VFI4-A/VFI402Q.Seq.d/                       1382   0.0
SFK585 (SFK585Q) /CSM/SF/SFK5-D/SFK585Q.Seq.d/                       1382   0.0
AFM216 (AFM216Q) /CSM/AF/AFM2-A/AFM216Q.Seq.d/                       1382   0.0
CFF110 (CFF110Q) /CSM/CF/CFF1-A/CFF110Q.Seq.d/                       1380   0.0
SFE854 (SFE854Q) /CSM/SF/SFE8-C/SFE854Q.Seq.d/                       1376   0.0
VFM246 (VFM246Q) /CSM/VF/VFM2-B/VFM246Q.Seq.d/                       1374   0.0
SFH192 (SFH192Q) /CSM/SF/SFH1-D/SFH192Q.Seq.d/                       1374   0.0
VFI896 (VFI896Q) /CSM/VF/VFI8-D/VFI896Q.Seq.d/                       1368   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M19937|M19937.1 Slime mold (D.discoideum) S-adenosyl-L-homocysteine hydrolase mRNA, complete cds.  1346  0.0  2
X12523|X12523.1 D. discoideum mRNA fragment for S-adenosyl-L-homocysteine hydrolase (EC 3.3.1.1).   513  0.0  3
AL399016|AL399016.1 T7 end of clone AS0AA012E07 of library AS0AA from strain CLIB 533 of Saccharomyces bayanus.   129  2e-62  7
AB004537|AB004537.1 Schizosaccharomyces pombe 37 kb genomic DNA, clone c213.    88  2e-60  8
AL022072|AL022072.1 S.pombe chromosome II cosmid c8D2.            88  5e-60  8
BE580064|BE580064.1 kq36f02.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:K02F2.2 CE17154 S-ADENOSYLHOMOCYSTEINE HYDROLASE ;, mRNA sequence.   133  6e-50  5
BE580043|BE580043.1 kq36c04.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:K02F2.2 CE17154 S-ADENOSYLHOMOCYSTEINE HYDROLASE ;, mRNA sequence.   133  1e-49  5
BF015319|BF015319.1 kq62e12.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to SW:SAHH_CAEEL P27604 ADENOSYLHOMOCYSTEINASE ;, mRNA sequence.   133  5e-48  4
BG227111|BG227111.1 kp97b11.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:K02F2.2 CE17154 S-ADENOSYLHOMOCYSTEINE HYDROLASE ;, mRNA sequence.   133  8e-48  4
U18796|U18796.1 Saccharomyces cerevisiae chromosome V cosmids 9379, 9581, and lambda clone 4678.    68  3e-45  8
dna update 2003. 9.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27655(A27655;S06394) adenosylhomocysteinase (EC 3.3.1.1) - slim...   820   0.0
(P27604) RecName: Full=Adenosylhomocysteinase;          Short=Ad...   662   0.0
DQ440384_1(DQ440384|pid:none) Aedes aegypti clone AET-5159 S-ade...   662   0.0
AY398307_1(AY398307|pid:none) Danio rerio clone RK113A3C03 S-ade...   661   0.0
(Q27580) RecName: Full=Adenosylhomocysteinase;          Short=Ad...   660   0.0
DQ167408_1(DQ167408|pid:none) Volvariella volvacea S-adenosyl-L-...   657   0.0
BC135905_1(BC135905|pid:none) Xenopus tropicalis S-adenosylhomoc...   654   0.0
BT043699_1(BT043699|pid:none) Salmo salar clone HM5_2286 S-adeno...   652   0.0
(P51893) RecName: Full=Adenosylhomocysteinase A;          Short=...   651   0.0
X95636_1(X95636|pid:none) D.melanogaster mRNA for S-adenosyl-L-h...   651   0.0
protein update 2009. 6.19
PSORT 

 psg: 0.53  gvh: 0.24  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.41  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.12  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: mitochondrial
	 32.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: cytoskeletal

>> prediction for VFF224 is mit
5' end seq. ID VFF224F
5' end seq. 
>VFF224F.Seq
TTCAGCGTTTAAACATACACACTTAAGAGAATGACTAAATTACACTACAAAGTTAAAGAT
ATTTCACTTGCCGCTTGGGGTCGTAAGGAAATTGAAATTGCCGAAAATGAAATGCCAGGT
TTAATGACCTTAAGAAAGAAATATGGTCCAGCTCAAATCTTAAAAGGTGCTCGTATTGCA
GGTTGTTTACACATGACTATCCAAACCGCCGTTTTAATTGAAACTTTAACTGCTCTCGGT
GCTCAAGTCCAATGGTCATCATGTAACATTTTCTCCACTCAAGATCAAGCCGCCGCTGCC
ATCGCTGCCACTGGTGTCCCAGTCTATGCCTGGAAAGGTGAAACCGAAGAAGAATACAAC
TGGTGTGTCGAACAAACCATTGTTTTCCAAGATGGTCAACCATTAAATATGATCTTAGAT
GATGGTGGTGATTTAACCAACCTCGTCCACGAGAAATACCCACAATTCTTAGCTGGTATC
AAAGGTATCTCTGAAGAAACCACCACTGGTGTCCACAACCTCTACAAAATGTTCAAAGAA
GGTAAATTAAAGGTCCCAGCCATCAACGTCAATGACTCTGTCACCAAATCCAAATTCGAT
AACTTATATGGTTGTCGTGAATCTTTAATCGATGGTATTAAACGTGCCA----------
Length of 5' end seq. 649
3' end seq. ID VFF224Z
3' end seq. 
>VFF224Z.Seq
----------ATCTTTAATCGATGGTATTAAACGTGCCACCGATGTTATGATTGCCGGTA
AAGTTGCCGTCGTCGCTGGTTACGGTGATGTAGGTAAAGGTTGTGCTCAATCATTATCAA
AAATGGGTGCTCGTGTTTTAGTCACTGAAATCGATCCAATCAATGCCCTCCAAGCCTGTA
TGGATGGTTACCAAATCGTCACCATGGAAACCGCCGCTCCATTATCAAACATTTTCGTCA
CCACCACCGGTTGTCGTGATATCGTCAGAGGTGAACACTTTGCCGTCATGAAAGAAGATG
CCATCGTTTGTAACATTGGTCACTTTGATTGTGAAATCGATGTCGCTTGGTTAAACGCCA
ACGCCAAAAAAGATACCGTCAAACCACAAGTTGACCGTTACACCCTTGCCAACGGTGTCC
ACATCATCCTCTTAGCTGAAGGTCGTCTCGTCAATTTAGGTTGTGGTACTGGTCATCCAT
CTTTTGTTATGTCAAACTCTTTCTGTAACCAGACTTTAGCTCAAATCGCCCTCTGGACTA
AAACTGAAGAATACCCATTAGGTGTCCACTTCTTACCAAAGATTTTAGATGAAGAAGTTG
CTCGTTTACATTTAGATCAATTAGGTGCTAAATTAACTACCCTCACTGAAAAACAATCCG
AATATTTATCAGTTCCAGTCGCTGGTCCATACAAAGTNATCACTACAGAATTAAAATCTT
T
Length of 3' end seq. 711
Connected seq. ID VFF224P
Connected seq. 
>VFF224P.Seq
TTCAGCGTTTAAACATACACACTTAAGAGAATGACTAAATTACACTACAAAGTTAAAGAT
ATTTCACTTGCCGCTTGGGGTCGTAAGGAAATTGAAATTGCCGAAAATGAAATGCCAGGT
TTAATGACCTTAAGAAAGAAATATGGTCCAGCTCAAATCTTAAAAGGTGCTCGTATTGCA
GGTTGTTTACACATGACTATCCAAACCGCCGTTTTAATTGAAACTTTAACTGCTCTCGGT
GCTCAAGTCCAATGGTCATCATGTAACATTTTCTCCACTCAAGATCAAGCCGCCGCTGCC
ATCGCTGCCACTGGTGTCCCAGTCTATGCCTGGAAAGGTGAAACCGAAGAAGAATACAAC
TGGTGTGTCGAACAAACCATTGTTTTCCAAGATGGTCAACCATTAAATATGATCTTAGAT
GATGGTGGTGATTTAACCAACCTCGTCCACGAGAAATACCCACAATTCTTAGCTGGTATC
AAAGGTATCTCTGAAGAAACCACCACTGGTGTCCACAACCTCTACAAAATGTTCAAAGAA
GGTAAATTAAAGGTCCCAGCCATCAACGTCAATGACTCTGTCACCAAATCCAAATTCGAT
AACTTATATGGTTGTCGTGAATCTTTAATCGATGGTATTAAACGTGCCA----------A
TCTTTAATCGATGGTATTAAACGTGCCACCGATGTTATGATTGCCGGTAAAGTTGCCGTC
GTCGCTGGTTACGGTGATGTAGGTAAAGGTTGTGCTCAATCATTATCAAAAATGGGTGCT
CGTGTTTTAGTCACTGAAATCGATCCAATCAATGCCCTCCAAGCCTGTATGGATGGTTAC
CAAATCGTCACCATGGAAACCGCCGCTCCATTATCAAACATTTTCGTCACCACCACCGGT
TGTCGTGATATCGTCAGAGGTGAACACTTTGCCGTCATGAAAGAAGATGCCATCGTTTGT
AACATTGGTCACTTTGATTGTGAAATCGATGTCGCTTGGTTAAACGCCAACGCCAAAAAA
GATACCGTCAAACCACAAGTTGACCGTTACACCCTTGCCAACGGTGTCCACATCATCCTC
TTAGCTGAAGGTCGTCTCGTCAATTTAGGTTGTGGTACTGGTCATCCATCTTTTGTTATG
TCAAACTCTTTCTGTAACCAGACTTTAGCTCAAATCGCCCTCTGGACTAAAACTGAAGAA
TACCCATTAGGTGTCCACTTCTTACCAAAGATTTTAGATGAAGAAGTTGCTCGTTTACAT
TTAGATCAATTAGGTGCTAAATTAACTACCCTCACTGAAAAACAATCCGAATATTTATCA
GTTCCAGTCGCTGGTCCATACAAAGTNATCACTACAGAATTAAAATCTTT
Length of connected seq. 1360
Full length Seq ID -
Full length Seq. -
Length of full length seq. -