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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFF201
Library VF
(Link to library)
Clone ID VFF201
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VFF201P
(Link to Original site)
Representative DNA sequence 
>VFF201 (VFF201Q) /CSM/VF/VFF2-A/VFF201Q.Seq.d/
GTAGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGG
AAAGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGC
AGTCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGA
AGTTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAAT
GTCAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGA
ACATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAA
ACATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGC
ATTGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTT
TTATTTAAATTGTTTCTTGGCAATCXXXXXXXXXXAAATGGTGCAATTCGTGTTAGGTGT
CGCCACAAATTTCGATAAGGGCCGCTTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCC
GTTTCATCCTTCCACTCCGTTATCATAGCTTTACAGATTTAATTTGTTATTTCCTCATTG
CTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAG
ATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGAAGATT
GGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCACTCCTTTGTACCT
TTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAG
ATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTT
ACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACACATTAATTACCTTTACAAAA
TGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCTCAAAAGANATTAAATAAATAA
CTTAAAAACCAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
*yflyiy**NCNKMSKVITGKQYSWSELAKHNTENDCWVAVDGKVYDITRWVPLHPGGKE
VLLLAAGRDVTNLFESYHPMSDKPTSILKNYEIGYISSYEHPKFVQKSDFYKTLKERVRK
HFKATDQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAI---

---MVQFVLGVATNFDKGRLRHW*ivfcflpfhpstpls*lyrfnllfphc*irlwlvsh
n*fps*scr*rsqilcyp*ktr*tisnq*rlgnpst*nysrlwswftplylf*wffksss
cssfipincsrflpttctnckrsl*rt*hylph*tklh*syyvth*lplqng**srlc*k
tislkrx*innlktn
Translated Amino Acid sequence (All Frames) 
Frame A:
vvfsvhiliel**nvksnyrkaillvrvsqt*h*k*llgcsrw*sl*yykmgtitsrw*r
sftfssr*rcykfi*klssnvr*tninfkel*nwiyfii*tskicskirflqdikres*k
tf*snrsrstngs*yfltfsiglsfsvcnllfstlyq*qflfklflgn---

---kwcnsc*vspqisiraayvigklsfvffrfilplryhsftdlicyfliaefvfgwyl
tinfqvshvaedlkffatperpdepsqinedwailqlkttqdyghgsllctffsgslnhq
vvhhlfpsiaqdfypqlvpivkevckehnityhikpnfteaimshinylykmgndpdyvk
kplaskxik*it*kp

Frame B:
*yflyiy**NCNKMSKVITGKQYSWSELAKHNTENDCWVAVDGKVYDITRWVPLHPGGKE
VLLLAAGRDVTNLFESYHPMSDKPTSILKNYEIGYISSYEHPKFVQKSDFYKTLKERVRK
HFKATDQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAI---

---ngairvrcrhkfr*gpltslvncllfssvssfhsviialqi*fvissllnsslvgis
qlisklvmslkisnsllplkdqmnhlksmkigqsfnlkllkimvmvhsfvpflvvl*iik
lfiiyshqllkisthnlyql*kkfvknitllttlnqtslkllchtlitftkwvmiqimlk
nh*pqkxlnk*lknq

Frame C:
sifctyinrivikcqk*lqesntlgqs*pnitlkmivglqsmvkfmilqdgyhyiqvvkk
fyf*qqvemlqiylkviiqcqinqhqf*rimkldifhhmniqnlfknqistrh*krelen
ilkqpikihkwqlvfshv*hwfif*cl*lti*hiipvtifi*ivswq---

---MVQFVLGVATNFDKGRLRHW*ivfcflpfhpstpls*lyrfnllfphc*irlwlvsh
n*fps*scr*rsqilcyp*ktr*tisnq*rlgnpst*nysrlwswftplylf*wffksss
cssfipincsrflpttctnckrsl*rt*hylph*tklh*syyvth*lplqng**srlc*k
tislkrx*innlktn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF201 (VFF201Q) /CSM/VF/VFF2-A/VFF201Q.Seq.d/                       2101   0.0
VFM295 (VFM295Q) /CSM/VF/VFM2-D/VFM295Q.Seq.d/                       1102   0.0
VFN880 (VFN880Q) /CSM/VF/VFN8-D/VFN880Q.Seq.d/                       1100   0.0
VFN123 (VFN123Q) /CSM/VF/VFN1-A/VFN123Q.Seq.d/                       1092   0.0
VFK616 (VFK616Q) /CSM/VF/VFK6-A/VFK616Q.Seq.d/                       1088   0.0
VSH813 (VSH813Q) /CSM/VS/VSH8-A/VSH813Q.Seq.d/                       1082   0.0
SSB813 (SSB813Q) /CSM/SS/SSB8-A/SSB813Q.Seq.d/                       1080   0.0
SLE621 (SLE621Q) /CSM/SL/SLE6-A/SLE621Q.Seq.d/                       1080   0.0
SLA259 (SLA259Q) /CSM/SL/SLA2-C/SLA259Q.Seq.d/                       1080   0.0
SLA256 (SLA256Q) /CSM/SL/SLA2-C/SLA256Q.Seq.d/                       1080   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BD092935|BD092935.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants.  1005  0.0  4
BD082645|BD082645.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids.  1005  0.0  4
BD082630|BD082630.1 Methods and compositions for synthesis of long chain poly-unsaturated fatty acids.  1005  0.0  4
AB029311|AB029311.1 Dictyostelium discoideum Dd des5 gene for fatty acid desaturase, complete cds.   710  0.0  7
AB022097|AB022097.1 Dictyostelium discoideum mRNA for delta 5 fatty acid desaturase, complete cds.   149  e-105  10
AL355612|AL355612.8 Human DNA sequence from clone RP11-343P23 on chromosome 6 Contains  GSSs.    42  0.10  3
AX347081|AX347081.1 Sequence 2152 from Patent WO0200928.          48  0.23  1
AC117308|AC117308.7 Rattus norvegicus clone CH230-216M16, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    48  0.23  1
AC117128|AC117128.8 Rattus norvegicus clone CH230-230O8, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    48  0.23  1
AC129112|AC129112.4 Rattus norvegicus clone CH230-265O9, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    48  0.23  1
dna update 2003. 9.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   355   2e-96
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   248   3e-64
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   206   9e-52
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   138   4e-31
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   136   1e-30
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   135   2e-30
CP000592_278(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   130   6e-29
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   119   2e-25
CP001574_328(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   117   7e-25
CS020137_1(CS020137|pid:none) Sequence 91 from Patent WO20050123...   116   1e-24
protein update 2009. 6.19
PSORT 

 psg: 0.70  gvh: 0.24  alm: 0.25  top: 0.63  tms: 0.13  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 24.0 %: endoplasmic reticulum
	 20.0 %: plasma membrane
	 16.0 %: nuclear
	 16.0 %: vacuolar
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for VFF201 is end
5' end seq. ID VFF201F
5' end seq. 
>VFF201F.Seq
GTAGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGG
AAAGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGC
AGTCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGA
AGTTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAAT
GTCAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGA
ACATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAA
ACATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGC
ATTGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTT
TTATTTAAATTGTTTCTTGGCAATC----------
Length of 5' end seq. 505
3' end seq. ID VFF201Z
3' end seq. 
>VFF201Z.Seq
----------AAATGGTGCAATTCGTGTTAGGTGTCGCCACAAATTTCGATAAGGGCCGC
TTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTATCA
TAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTATCT
CACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTGA
AAGACCAGATGAACCATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTAAAACTAC
TCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATCA
AGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTACCAAT
TGTAAAAGAAGTTTGTAAAGAACATAACATTACTTACCACATTAAACCAAACTTCACTGA
AGCTATTATGTCACACATTAATTACCTTTACAAAATGGGTAATGATCCAGATTATGTTAA
AAAACCATTAGCCTCAAAAGANATTAAATAAATAACTTAAAAACCAAT
Length of 3' end seq. 578
Connected seq. ID VFF201P
Connected seq. 
>VFF201P.Seq
GTAGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGG
AAAGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGC
AGTCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGA
AGTTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAAT
GTCAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGA
ACATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAA
ACATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGC
ATTGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTT
TTATTTAAATTGTTTCTTGGCAATC----------AAATGGTGCAATTCGTGTTAGGTGT
CGCCACAAATTTCGATAAGGGCCGCTTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCC
GTTTCATCCTTCCACTCCGTTATCATAGCTTTACAGATTTAATTTGTTATTTCCTCATTG
CTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAG
ATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGAAGATT
GGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCACTCCTTTGTACCT
TTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAG
ATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTT
ACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACACATTAATTACCTTTACAAAA
TGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCTCAAAAGANATTAAATAAATAA
CTTAAAAACCAAT
Length of connected seq. 1083
Full length Seq ID -
Full length Seq. -
Length of full length seq. -