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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFF191
Library VF
(Link to library)
Clone ID VFF191
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16447-1
Original site URL
Representative seq. ID VFF191P
(Link to Original site)
Representative DNA sequence 
>VFF191 (VFF191Q) /CSM/VF/VFF1-D/VFF191Q.Seq.d/
AATTCAGCACAAACACTACTACCTAAAAAATGACACAACCAAATAACTATAAAATTGGAA
CCAACGTTATCCACGCTGGTCAATCCGCTGATAAAAATACAGGTGCTGTAATTGTACCAA
TTTCATTATCCACCACTTTCTTACAACCATCACCAGGTGTACTNCATTCTGAAXXXXXXX
XXXTTATGCACTCTGTCACCAAATATATCAATGGTCATAGTGATTGCGTTATGGGTGTTT
TGGCAACAAACAACGATGAACTCTACGCCAAGCTTAAATTCCTCCAAAATAGTATTGGTG
CCGTGCCATCACCATTCGATTGTTTCCTCGCCCTTCGTGGTTTGAAGACTCTCCACGTTC
GTATGGAAGCTCATCAAAAGAATGCTTTCGCCATTTGCAATTTCCTCGAAAAACATCCAA
AAGTTGAAAGAGTCATCTATCCAGGTCTCCCATCTCATCCACAACATGAGATTTGCAAAC
GTCAAATGAAAGGTTATGGTGGTATGGTTGTTTTCTTTGTTAAAGGTTCAATCGATCAAA
GCAGATCTTTCTTGGAGAATATTAAACTCTTTGCTCTTGCTGAATCTTTAGGTGGTGTCG
AATCTTTAATTGAACTTCCATCAGTTATGACTCACGCAAGTGTTCCAGCTGAAGAAAGAG
CTAAACTCGGTATCTCTGATACTTTATTCGTTTATCCGTTGGTATTAAGATATTAANNAT
CTTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
fstnttt*KMTQPNNYKIGTNVIHAGQSADKNTGAVIVPISLSTTFLQPSPGVLHSE---

---MHSVTKYINGHSDCVMGVLATNNDELYAKLKFLQNSIGAVPSPFDCFLALRGLKTLH
VRMEAHQKNAFAICNFLEKHPKVERVIYPGLPSHPQHEICKRQMKGYGGMVVFFVKGSID
QSRSFLENIKLFALAESLGGVESLIELPSVMTHASVPAEERAKLGISDTLFVYPLVLRY*
xs
Translated Amino Acid sequence (All Frames) 
Frame A:
nsaqtllpkk*hnqitikleptlstlvnplikiqvl*lyqfhypplsynhhqvyxil---

---lctlspnismvivialwvfwqqttmnstpslnsskivlvpchhhsivsspfvv*rls
tfvwklikrmlspfaisskniqklkessiqvshlihnmrfanvk*kvmvvwlfsllkvqs
ikadlswrilnsllllnl*vvsnl*lnfhql*ltqvfqlkkelnsvslilysfirwy*di
xxl

Frame B:
iqhkhyylkndttk*l*nwnqryprwsir**kyrccnctnfiihhflttitrctxf*---

---yalchqiyqws**lrygcfgnkqr*tlrqa*ippk*ywcraitirlfprpswfedsp
rsygssskecfrhlqfprktsks*kshlsrspisstt*dlqtsnerlwwygcflc*rfnr
skqiflgey*tlcsc*ifrwcrifn*tsisydsrkcss*rks*tryl*yfirlsvgikil
xif

Frame C:
fstnttt*KMTQPNNYKIGTNVIHAGQSADKNTGAVIVPISLSTTFLQPSPGVLHSE---

---MHSVTKYINGHSDCVMGVLATNNDELYAKLKFLQNSIGAVPSPFDCFLALRGLKTLH
VRMEAHQKNAFAICNFLEKHPKVERVIYPGLPSHPQHEICKRQMKGYGGMVVFFVKGSID
QSRSFLENIKLFALAESLGGVESLIELPSVMTHASVPAEERAKLGISDTLFVYPLVLRY*
xs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF191 (VFF191Q) /CSM/VF/VFF1-D/VFF191Q.Seq.d/                       1358   0.0
VFJ884 (VFJ884Q) /CSM/VF/VFJ8-D/VFJ884Q.Seq.d/                       1051   0.0
VFL196 (VFL196Q) /CSM/VF/VFL1-D/VFL196Q.Seq.d/                       1043   0.0
CFG346 (CFG346Q) /CSM/CF/CFG3-B/CFG346Q.Seq.d/                       1039   0.0
SFH354 (SFH354Q) /CSM/SF/SFH3-C/SFH354Q.Seq.d/                       1037   0.0
SFE327 (SFE327Q) /CSM/SF/SFE3-B/SFE327Q.Seq.d/                       1037   0.0
SFE310 (SFE310Q) /CSM/SF/SFE3-A/SFE310Q.Seq.d/                       1037   0.0
CFK279 (CFK279Q) /CSM/CF/CFK2-D/CFK279Q.Seq.d/                       1037   0.0
CFG125 (CFG125Q) /CSM/CF/CFG1-B/CFG125Q.Seq.d/                       1035   0.0
VFO896 (VFO896Q) /CSM/VF/VFO8-D/VFO896Q.Seq.d/                       1033   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U68249|U68249.1 Dictyostelium discoideum CysA (cysA) mRNA, partial cds.   272  6e-69  1
CB404981|CB404981.1 OSTR034E8_1 AD-wrmcDNA Caenorhabditis elegans cDNA, mRNA sequence.    52  0.010  1
AU210889|AU210889.1 Caenorhabditis elegans cDNA clone:yk766b01 : 3' end, single read.    52  0.010  1
U58758|U58758.2 Caenorhabditis elegans cosmid ZK1127, complete sequence.    52  0.010  1
BJ104383|BJ104383.1 Caenorhabditis elegans cDNA clone:yk1061b03 : 5' end, single read.    52  0.010  1
BQ125060|BQ125060.1 pb31b09.y1 Anc caninum L3 serum stim pAMP1 v1 Chiapelli McCarter Ancylostoma caninum cDNA 5' similar to SW:CGL_CAEEL P55216 PUTATIVE CYSTATHIONINE GAMMA-LYASE ;, mRNA sequence.    52  0.010  1
BJ132004|BJ132004.1 Caenorhabditis elegans cDNA clone:yk1061b03 : 3' end, single read.    52  0.010  1
AF170901|AF170901.1 Lactococcus lactis subsp. cremoris MG1363 cystathionine beta/gamma-lyase (metC) gene, complete cds.    48  0.15  1
AF131880|AF131880.2 Lactococcus lactis subsp. cremoris strain MG1363 cystathionine beta-lyase MetC (metC) and O-acetylserine sulfhydrylase CysK (cysK) genes, complete cds.    48  0.15  1
AE006311|AE006311.1 Lactococcus lactis subsp. lactis IL1403 section 73 of 218 of the complete genome.    48  0.15  1
dna update 2003. 9.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55DV9) RecName: Full=Cystathionine gamma-lyase;          EC=4....   339   6e-92
(Q8VCN5) RecName: Full=Cystathionine gamma-lyase;          EC=4....   235   1e-60
BT082982_1(BT082982|pid:none) Anoplopoma fimbria clone afim-evh-...   234   2e-60
BC067624_1(BC067624|pid:none) Danio rerio cystathionase (cystath...   234   3e-60
BC082653_1(BC082653|pid:none) Xenopus laevis hypothetical LOC494...   233   4e-60
(P18757) RecName: Full=Cystathionine gamma-lyase;          EC=4....   233   6e-60
EU315111_1(EU315111|pid:none) Oncorhynchus mykiss cystathionine ...   232   8e-60
BC151523_1(BC151523|pid:none) Bos taurus cystathionase (cystathi...   230   4e-59
CP000769_3804(CP000769|pid:none) Anaeromyxobacter sp. Fw109-5, c...   229   8e-59
(Q60HG7) RecName: Full=Cystathionine gamma-lyase;          EC=4....   228   1e-58
protein update 2009. 6.19
PSORT 

 psg: 0.80  gvh: 0.54  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 40.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for VFF191 is cyt
5' end seq. ID VFF191F
5' end seq. 
>VFF191F.Seq
AATTCAGCACAAACACTACTACCTAAAAAATGACACAACCAAATAACTATAAAATTGGAA
CCAACGTTATCCACGCTGGTCAATCCGCTGATAAAAATACAGGTGCTGTAATTGTACCAA
TTTCATTATCCACCACTTTCTTACAACCATCACCAGGTGTACTNCATTCTGAA-------
---
Length of 5' end seq. 173
3' end seq. ID VFF191Z
3' end seq. 
>VFF191Z.Seq
----------TTATGCACTCTGTCACCAAATATATCAATGGTCATAGTGATTGCGTTATG
GGTGTTTTGGCAACAAACAACGATGAACTCTACGCCAAGCTTAAATTCCTCCAAAATAGT
ATTGGTGCCGTGCCATCACCATTCGATTGTTTCCTCGCCCTTCGTGGTTTGAAGACTCTC
CACGTTCGTATGGAAGCTCATCAAAAGAATGCTTTCGCCATTTGCAATTTCCTCGAAAAA
CATCCAAAAGTTGAAAGAGTCATCTATCCAGGTCTCCCATCTCATCCACAACATGAGATT
TGCAAACGTCAAATGAAAGGTTATGGTGGTATGGTTGTTTTCTTTGTTAAAGGTTCAATC
GATCAAAGCAGATCTTTCTTGGAGAATATTAAACTCTTTGCTCTTGCTGAATCTTTAGGT
GGTGTCGAATCTTTAATTGAACTTCCATCAGTTATGACTCACGCAAGTGTTCCAGCTGAA
GAAAGAGCTAAACTCGGTATCTCTGATACTTTATTCGTTTATCCGTTGGTATTAAGATAT
TAANNATCTTT
Length of 3' end seq. 541
Connected seq. ID VFF191P
Connected seq. 
>VFF191P.Seq
AATTCAGCACAAACACTACTACCTAAAAAATGACACAACCAAATAACTATAAAATTGGAA
CCAACGTTATCCACGCTGGTCAATCCGCTGATAAAAATACAGGTGCTGTAATTGTACCAA
TTTCATTATCCACCACTTTCTTACAACCATCACCAGGTGTACTNCATTCTGAA-------
---TTATGCACTCTGTCACCAAATATATCAATGGTCATAGTGATTGCGTTATGGGTGTTT
TGGCAACAAACAACGATGAACTCTACGCCAAGCTTAAATTCCTCCAAAATAGTATTGGTG
CCGTGCCATCACCATTCGATTGTTTCCTCGCCCTTCGTGGTTTGAAGACTCTCCACGTTC
GTATGGAAGCTCATCAAAAGAATGCTTTCGCCATTTGCAATTTCCTCGAAAAACATCCAA
AAGTTGAAAGAGTCATCTATCCAGGTCTCCCATCTCATCCACAACATGAGATTTGCAAAC
GTCAAATGAAAGGTTATGGTGGTATGGTTGTTTTCTTTGTTAAAGGTTCAATCGATCAAA
GCAGATCTTTCTTGGAGAATATTAAACTCTTTGCTCTTGCTGAATCTTTAGGTGGTGTCG
AATCTTTAATTGAACTTCCATCAGTTATGACTCACGCAAGTGTTCCAGCTGAAGAAAGAG
CTAAACTCGGTATCTCTGATACTTTATTCGTTTATCCGTTGGTATTAAGATATTAANNAT
CTTT
Length of connected seq. 714
Full length Seq ID -
Full length Seq. -
Length of full length seq. -