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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFF135
Library VF
(Link to library)
Clone ID VFF135
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16605-1
Original site URL
Representative seq. ID VFF135P
(Link to Original site)
Representative DNA sequence 
>VFF135 (VFF135Q) /CSM/VF/VFF1-B/VFF135Q.Seq.d/
ATCAATTAAATATATATATACTAAAAATGAGAGTTTTATCATTCCTTTGTTTATTATTAG
TTAGCTACGCTTCTGCTAAACAACAATTCTCTGAATTACAATACAGAAATGCTTTCACCA
ACTGGATGCAAGCTCACCAAAGAACTTATTCCTCTGAAGAATTTAATGCTCGTTATCAAA
TCTTCAAATCCAATATGGATTATGTACACCAATGGAATTCAAAAGGTGGTGAAACCGTTT
TGGGTTTAAATGTTTTCGCTGATATTACCAACCAAGAATATAGAACTACCTACTTGGGTA
CCCCATTCGATGGTTCAGCCCTCATTGGTACTGAAGAAGAGAAAATCTTCTCCACCCCAG
CCCCAACTGTTGATTGGAGAGCTCAAGGTGCTGTCACACCAATTAAAAATCAAGGTCAAT
GTGGTGGCTGCTGGTCATTCTCAACCACTGGTTCAACTGAAGGTGCTCACTTTATTGCAT
CTGGAACAAAAAAAGATTTAGTTTCATTATCTGAACAAAACTTGATCGATTGTTCAAAAT
CATACGGTAXXXXXXXXXXTGAAGCTTCATTACAATCAGCATCAAACAATGCTCCAGTCT
CTGTTGCAATTGATGCTTCAAATGAATCCTTCCAATTATATGAATCAGGTATCTACTATG
AACCAGCATGTTCTCCAACCCAACTTGATCATGGTGTTTTAGTTGTTGGTTATGGTTCAG
GTTCAAGTTCATCATCTGGTTCATCATCTGGTAAATCATCATCATCATCATCAACTGGTG
GTAAAACTTCATCCTCATCATCATCAGGTAAAGCTTCATCATCATCATCAGGCAAAGCTT
CATCATCATCATCATCAGGTAAAACTTCATCTGCTGCTTCATCAACCTCTGGTTCTCAAT
CAGGTTCCCAATCAGGTAGCCAATCAGGCCAATCCACCGGTTCACAATCAGGTCAAACCT
CTGCTTCTGGTCAAGCATCAGCATCAGGTTCTGGTTCTGGCTCAGGTTCAGGTTCAGGTT
CAGGTTCAGGCTCAGGTGCTGTTGAGGCCTCATCTGGTAACTACTGGATCGTTAAAAACT
CATGGGGTACTTCATGGGGTATGGATGGTTACATTTTTATGAGCAAAGATAGAAATAACA
ATTGTGGTATCGCAACAATGGCTTCTTTCCCAACTGCCTCATCAAATTAAAATTTTATTT
TTAATTGTCCGACTAACATTAATTTATTNTAATAAATAAAAAAAATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
QLNIYILKMRVLSFLCLLLVSYASAKQQFSELQYRNAFTNWMQAHQRTYSSEEFNARYQI
FKSNMDYVHQWNSKGGETVLGLNVFADITNQEYRTTYLGTPFDGSALIGTEEEKIFSTPA
PTVDWRAQGAVTPIKNQGQCGGCWSFSTTGSTEGAHFIASGTKKDLVSLSEQNLIDCSKS
YG---

---EASLQSASNNAPVSVAIDASNESFQLYESGIYYEPACSPTQLDHGVLVVGYGSGSSS
SSGSSSGKSSSSSSTGGKTSSSSSSGKASSSSSGKASSSSSSGKTSSAASSTSGSQSGSQ
SGSQSGQSTGSQSGQTSASGQASASGSGSGSGSGSGSGSGSGAVEASSGNYWIVKNSWGT
SWGMDGYIFMSKDRNNNCGIATMASFPTASSN*nfifncptninlx**ikki
Translated Amino Acid sequence (All Frames) 
Frame A:
in*iyiy*k*efyhsfvyy*latlllnnnslnyntemlsptgckltkeliplknlmlvik
ssnpiwimytngiqkvvkpfwv*mfslilptknielptwvphsmvqpslvlkkrkssppq
pqlligelkvlshqlkikvnvvaaghsqplvqlkvltllhleqkki*fhylnkt*sivqn
htv---

---*sfitisikqcsslccn*cfk*ilpii*iryll*tsmfsnpt*swcfscwlwfrfkf
iiwfiiw*iiiiiinww*nfiliiir*sfiiiirqsfiiiiir*nficcfinlwfsirfp
ir*pirpihrftirsnlcfwssisirfwfwlrfrfrfrfrlrcc*gliw*lldr*klmgy
fmgygwlhfyeqr*k*qlwyrnngffpncliklkfyf*lsd*h*fixinkkn

Frame B:
sikyiytknesfiiplfiis*lrfc*ttil*itiqkcfhqldasspknlfl*ri*cslsn
lqiqyglctpmefkrw*nrfgfkcfr*yyqpri*nyllgypirwfsphwy*rrenllhps
pnc*lessrcchtn*ksrsmwwllvilnhwfn*rcslyciwnkkrfsfii*tkldrlfki
ir---

---EASLQSASNNAPVSVAIDASNESFQLYESGIYYEPACSPTQLDHGVLVVGYGSGSSS
SSGSSSGKSSSSSSTGGKTSSSSSSGKASSSSSGKASSSSSSGKTSSAASSTSGSQSGSQ
SGSQSGQSTGSQSGQTSASGQASASGSGSGSGSGSGSGSGSGAVEASSGNYWIVKNSWGT
SWGMDGYIFMSKDRNNNCGIATMASFPTASSN*nfifncptninlx**ikki

Frame C:
QLNIYILKMRVLSFLCLLLVSYASAKQQFSELQYRNAFTNWMQAHQRTYSSEEFNARYQI
FKSNMDYVHQWNSKGGETVLGLNVFADITNQEYRTTYLGTPFDGSALIGTEEEKIFSTPA
PTVDWRAQGAVTPIKNQGQCGGCWSFSTTGSTEGAHFIASGTKKDLVSLSEQNLIDCSKS
YG---

---klhynqhqtmlqsllqlmlqmnpsnymnqvstmnqhvlqpnlimvf*llvmvqvqvh
hlvhhlvnhhhhhqlvvklhphhhqvklhhhhqaklhhhhhqvklhlllhqplvlnqvpn
qvanqanppvhnqvkplllvkhqhqvlvlaqvqvqvqvqaqvllrphlvttgslkthgvl
hgvwmvtfl*akieitivvsqqwllsqlphqikilflivrltliyxnk*kk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF135 (VFF135Q) /CSM/VF/VFF1-B/VFF135Q.Seq.d/                       1338   0.0
VFO306 (VFO306Q) /CSM/VF/VFO3-A/VFO306Q.Seq.d/                       1047   0.0
VFN628 (VFN628Q) /CSM/VF/VFN6-B/VFN628Q.Seq.d/                       1047   0.0
VFM544 (VFM544Q) /CSM/VF/VFM5-B/VFM544Q.Seq.d/                       1047   0.0
VFM320 (VFM320Q) /CSM/VF/VFM3-A/VFM320Q.Seq.d/                       1047   0.0
VFL463 (VFL463Q) /CSM/VF/VFL4-C/VFL463Q.Seq.d/                       1047   0.0
VFK619 (VFK619Q) /CSM/VF/VFK6-A/VFK619Q.Seq.d/                       1047   0.0
VFK603 (VFK603Q) /CSM/VF/VFK6-A/VFK603Q.Seq.d/                       1047   0.0
VFK133 (VFK133Q) /CSM/VF/VFK1-B/VFK133Q.Seq.d/                       1047   0.0
VFG723 (VFG723Q) /CSM/VF/VFG7-A/VFG723Q.Seq.d/                       1047   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

L36204|L36204.1 Dictyostelium discoideum cysteine proteinase (CP4) mRNA, complete cds.   922  0.0  6
L36205|L36205.1 Dictyostelium discoideum cysteine proteinase CP5 mRNA, complete cds.   121  e-124  9
U72746|U72746.1 Dictyostelium discoideum cysteine proteinase (cprG) mRNA, complete cds.   131  e-116  8
AC117072|AC117072.2 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.   121  e-109  11
U72745|U72745.1 Dictyostelium discoideum cysteine proteinase (cprF) mRNA, complete cds.   115  6e-95  7
X03344|X03344.1 Dictyostelium discoideum mRNA for cysteine proteinase 2.    76  6e-54  7
M16039|M16039.1 Dictyostelium discoideum pst-cath gene encoding pst-cathepsin, complete cds.    74  1e-47  9
X02407|X02407.1 D.discoideum mRNA for cysteine proteinase 1.      70  1e-18  3
BJ171907|BJ171907.1 Physcomitrella patens subsp. patens cDNA clone:pph30i20, 3' end, single read.    66  9e-12  2
BZ399359|BZ399359.1 EINDT41TF EI_10_12_KB Entamoeba invadens genomic clone EINDT41, DNA sequence.    52  2e-07  3
dna update 2003. 9.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

L36204_1(L36204|pid:none) Dictyostelium discoideum cysteine prot...   400   e-110
(P54640) RecName: Full=Cysteine proteinase 5;          EC=3.4.22...   315   2e-84
(Q94504) RecName: Full=Cysteine proteinase 7;          EC=3.4.22...   311   3e-83
L36205_1(L36205|pid:none) Dictyostelium discoideum cysteine prot...   308   2e-82
(Q94503) RecName: Full=Cysteine proteinase 6;          EC=3.4.22...   305   3e-81
(P04989) RecName: Full=Cysteine proteinase 2;          EC=3.4.22...   239   1e-61
EF053509_1(EF053509|pid:none) Acanthamoeba castellanii cysteine ...   192   3e-47
AC117076_20(AC117076|pid:none) Dictyostelium discoideum chromoso...   186   1e-45
DQ280314_1(DQ280314|pid:none) Hymeniacidon perlevis cathepsin L ...   160   1e-37
AY220615_1(AY220615|pid:none) Hydra vulgaris cathepsin L precurs...   156   1e-36
protein update 2009. 6.19
PSORT 

 psg: 0.96  gvh: 0.87  alm: 0.52  top: 0.40  tms: 0.00  mit: 0.36  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: extracellular, including cell wall
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoplasmic
	  4.0 %: Golgi

>> prediction for VFF135 is exc
5' end seq. ID VFF135F
5' end seq. 
>VFF135F.Seq
ATCAATTAAATATATATATACTAAAAATGAGAGTTTTATCATTCCTTTGTTTATTATTAG
TTAGCTACGCTTCTGCTAAACAACAATTCTCTGAATTACAATACAGAAATGCTTTCACCA
ACTGGATGCAAGCTCACCAAAGAACTTATTCCTCTGAAGAATTTAATGCTCGTTATCAAA
TCTTCAAATCCAATATGGATTATGTACACCAATGGAATTCAAAAGGTGGTGAAACCGTTT
TGGGTTTAAATGTTTTCGCTGATATTACCAACCAAGAATATAGAACTACCTACTTGGGTA
CCCCATTCGATGGTTCAGCCCTCATTGGTACTGAAGAAGAGAAAATCTTCTCCACCCCAG
CCCCAACTGTTGATTGGAGAGCTCAAGGTGCTGTCACACCAATTAAAAATCAAGGTCAAT
GTGGTGGCTGCTGGTCATTCTCAACCACTGGTTCAACTGAAGGTGCTCACTTTATTGCAT
CTGGAACAAAAAAAGATTTAGTTTCATTATCTGAACAAAACTTGATCGATTGTTCAAAAT
CATACGGTA----------
Length of 5' end seq. 549
3' end seq. ID VFF135Z
3' end seq. 
>VFF135Z.Seq
----------TGAAGCTTCATTACAATCAGCATCAAACAATGCTCCAGTCTCTGTTGCAA
TTGATGCTTCAAATGAATCCTTCCAATTATATGAATCAGGTATCTACTATGAACCAGCAT
GTTCTCCAACCCAACTTGATCATGGTGTTTTAGTTGTTGGTTATGGTTCAGGTTCAAGTT
CATCATCTGGTTCATCATCTGGTAAATCATCATCATCATCATCAACTGGTGGTAAAACTT
CATCCTCATCATCATCAGGTAAAGCTTCATCATCATCATCAGGCAAAGCTTCATCATCAT
CATCATCAGGTAAAACTTCATCTGCTGCTTCATCAACCTCTGGTTCTCAATCAGGTTCCC
AATCAGGTAGCCAATCAGGCCAATCCACCGGTTCACAATCAGGTCAAACCTCTGCTTCTG
GTCAAGCATCAGCATCAGGTTCTGGTTCTGGCTCAGGTTCAGGTTCAGGTTCAGGTTCAG
GCTCAGGTGCTGTTGAGGCCTCATCTGGTAACTACTGGATCGTTAAAAACTCATGGGGTA
CTTCATGGGGTATGGATGGTTACATTTTTATGAGCAAAGATAGAAATAACAATTGTGGTA
TCGCAACAATGGCTTCTTTCCCAACTGCCTCATCAAATTAAAATTTTATTTTTAATTGTC
CGACTAACATTAATTTATTNTAATAAATAAAAAAAATA
Length of 3' end seq. 688
Connected seq. ID VFF135P
Connected seq. 
>VFF135P.Seq
ATCAATTAAATATATATATACTAAAAATGAGAGTTTTATCATTCCTTTGTTTATTATTAG
TTAGCTACGCTTCTGCTAAACAACAATTCTCTGAATTACAATACAGAAATGCTTTCACCA
ACTGGATGCAAGCTCACCAAAGAACTTATTCCTCTGAAGAATTTAATGCTCGTTATCAAA
TCTTCAAATCCAATATGGATTATGTACACCAATGGAATTCAAAAGGTGGTGAAACCGTTT
TGGGTTTAAATGTTTTCGCTGATATTACCAACCAAGAATATAGAACTACCTACTTGGGTA
CCCCATTCGATGGTTCAGCCCTCATTGGTACTGAAGAAGAGAAAATCTTCTCCACCCCAG
CCCCAACTGTTGATTGGAGAGCTCAAGGTGCTGTCACACCAATTAAAAATCAAGGTCAAT
GTGGTGGCTGCTGGTCATTCTCAACCACTGGTTCAACTGAAGGTGCTCACTTTATTGCAT
CTGGAACAAAAAAAGATTTAGTTTCATTATCTGAACAAAACTTGATCGATTGTTCAAAAT
CATACGGTA----------TGAAGCTTCATTACAATCAGCATCAAACAATGCTCCAGTCT
CTGTTGCAATTGATGCTTCAAATGAATCCTTCCAATTATATGAATCAGGTATCTACTATG
AACCAGCATGTTCTCCAACCCAACTTGATCATGGTGTTTTAGTTGTTGGTTATGGTTCAG
GTTCAAGTTCATCATCTGGTTCATCATCTGGTAAATCATCATCATCATCATCAACTGGTG
GTAAAACTTCATCCTCATCATCATCAGGTAAAGCTTCATCATCATCATCAGGCAAAGCTT
CATCATCATCATCATCAGGTAAAACTTCATCTGCTGCTTCATCAACCTCTGGTTCTCAAT
CAGGTTCCCAATCAGGTAGCCAATCAGGCCAATCCACCGGTTCACAATCAGGTCAAACCT
CTGCTTCTGGTCAAGCATCAGCATCAGGTTCTGGTTCTGGCTCAGGTTCAGGTTCAGGTT
CAGGTTCAGGCTCAGGTGCTGTTGAGGCCTCATCTGGTAACTACTGGATCGTTAAAAACT
CATGGGGTACTTCATGGGGTATGGATGGTTACATTTTTATGAGCAAAGATAGAAATAACA
ATTGTGGTATCGCAACAATGGCTTCTTTCCCAACTGCCTCATCAAATTAAAATTTTATTT
TTAATTGTCCGACTAACATTAATTTATTNTAATAAATAAAAAAAATA
Length of connected seq. 1237
Full length Seq ID -
Full length Seq. -
Length of full length seq. -