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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFE845
Library VF
(Link to library)
Clone ID VFE845
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14349-1
Original site URL
Representative seq. ID VFE845P
(Link to Original site)
Representative DNA sequence 
>VFE845 (VFE845Q) /CSM/VF/VFE8-B/VFE845Q.Seq.d/
AACTTACTCAACTAAATAAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCT
CCAAACTGCGTTTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATC
TACAGAATCTCTGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGT
TCATTCGATGAATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGAC
TATCAATACAAAGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCA
GATACCGCCAACATTAAAAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAA
GCCTGTGTTGGTATCCAAGTTGAAATCCAAGGTACCGATGCCTCTGAAGTCAAAGATTCA
TGTTTCTATGAAAAATGTACCAAAATCTAAATAATTTGAAAAAATTTAAAAAAAAAAXXX
XXXXXXXAACTTACTCAACTAAATAAAAAACTATATATAAAAAATGTCTTCAGGTATTGC
TTTAGCTCCAAACTGCGTTTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGG
TATCATCTACAGAATCTCTGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAGC
TGGTTGTTCATTCGATGAATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTGT
CCTCGACTATCAATACAAAGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTG
GTGCCCAGATACCGCCAACATTAAAAAGAAAATGATGGCCACCTCATCAAAAGACTCTTT
ACGTAAAGCCTGTGTTGGTATCCAAGTTGAAATCCAAGGTACCGATGCCTNCTGAAGTCA
AAGATTCATGTTTCTATAAAAATGTACCAAAATCTAAATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ltqlnkklyiknvfrycfssklrfnlqrfkvrs*iwwyhlqnl*rfkrnhc*lhltswlf
ir*ihqmsprkrm*ircprlsiqrrrcpkeqnlfcclvpryrqh*kendghlikrlft*s
lcwyps*npryrcl*sqrfmfl*KMYQNLNNLKKFKKK---

---LTQLNKKLYIKNVFRYCFSSKLRFNLQRFKVRS*iwwyhlqnl*rfkrnhc*lhlts
wlfir*ihqmsprkrm*ircprlsiqrrrcpkeqnlfcclvpryrqh*kendghlikrlf
t*slcwyps*npryrcllkskihvsikmyqnln
Translated Amino Acid sequence (All Frames) 
Frame A:
nlln*iknyi*kmssgialapncvstfndlklgrkyggiiyrisddskeiivdstlpagc
sfdeftkclpenecryvvldyqykeegaqkskicfvawcpdtanikkkmmatsskdslrk
acvgiqveiqgtdasevkdscfyekctki*ii*knlkkk---

---nlln*iknyi*kmssgialapncvstfndlklgrkyggiiyrisddskeiivdstlp
agcsfdeftkclpenecryvvldyqykeegaqkskicfvawcpdtanikkkmmatsskds
lrkacvgiqveiqgtdax*sqrfmfl*kctki*i

Frame B:
tystk*ktiykkclqvll*lqtafqpsti*s*vvnmvvsstesltiqkkslltppyqlvv
hsmnspnvsqktnvdtlsstintkkkvpkraksvllpgaqipptlkrk*wpphqktlyvk
pvlvsklkskvpmplkskihvsmknvpksk*feki*kk---

---tystk*ktiykkclqvll*lqtafqpsti*s*vvnmvvsstesltiqkkslltppyq
lvvhsmnspnvsqktnvdtlsstintkkkvpkraksvllpgaqipptlkrk*wpphqktl
yvkpvlvsklkskvpmpxevkdscfyknvpksk

Frame C:
ltqlnkklyiknvfrycfssklrfnlqrfkvrs*iwwyhlqnl*rfkrnhc*lhltswlf
ir*ihqmsprkrm*ircprlsiqrrrcpkeqnlfcclvpryrqh*kendghlikrlft*s
lcwyps*npryrcl*sqrfmfl*KMYQNLNNLKKFKKK---

---LTQLNKKLYIKNVFRYCFSSKLRFNLQRFKVRS*iwwyhlqnl*rfkrnhc*lhlts
wlfir*ihqmsprkrm*ircprlsiqrrrcpkeqnlfcclvpryrqh*kendghlikrlf
t*slcwyps*npryrcllkskihvsikmyqnln
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ890 (VSJ890Q) /CSM/VS/VSJ8-D/VSJ890Q.Seq.d/                        835   0.0
VSJ584 (VSJ584Q) /CSM/VS/VSJ5-D/VSJ584Q.Seq.d/                        835   0.0
VSJ550 (VSJ550Q) /CSM/VS/VSJ5-C/VSJ550Q.Seq.d/                        835   0.0
VSJ437 (VSJ437Q) /CSM/VS/VSJ4-B/VSJ437Q.Seq.d/                        835   0.0
VSJ243 (VSJ243Q) /CSM/VS/VSJ2-B/VSJ243Q.Seq.d/                        835   0.0
VSI135 (VSI135Q) /CSM/VS/VSI1-B/VSI135Q.Seq.d/                        835   0.0
VSG665 (VSG665Q) /CSM/VS/VSG6-C/VSG665Q.Seq.d/                        835   0.0
VSD383 (VSD383Q) /CSM/VS/VSD3-D/VSD383Q.Seq.d/                        835   0.0
VSD320 (VSD320Q) /CSM/VS/VSD3-A/VSD320Q.Seq.d/                        835   0.0
VSA646 (VSA646Q) /CSM/VS/VSA6-B/VSA646Q.Seq.d/                        835   0.0
own update 2001.10.16
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

D37981|D37981.1 Dictyostelium discoidium DDCOF2 gene for cofilin, complete cds.   835  0.0  3
D37980|D37980.1 Dictyostelium discoidium DDCOF1 gene for cofilin, complete cds (exon1-2).   833  0.0  3
X04004|X04004.1 Dictyostelium discoideum mRNA for contact site A (csA) protein.   168  8e-38  1
AJ511931|AJ511931.1 Apodemus sylvaticus mitochondrial partial cytb gene for cytochrome  b, tissue library JRM-417.    46  0.79  1
AJ511932|AJ511932.1 Apodemus sylvaticus mitochondrial partial cytb gene for cytochrome  b, tissue library JRM-434.    46  0.79  1
AC143994|AC143994.1 Macaca mulatta clone CH250-272N23, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    38  0.81  2
AW399043|AW399043.1 EST309543 L. pennellii trichome, Cornell University Lycopersicon pennellii cDNA clone cLPT5H2 5', mRNA sequence.    34  0.98  2
AW332691|AW332691.1 S11F8 AGS-1 Pneumocystis carinii f. sp. carinii cDNA 3', mRNA sequence.    40  2.6  2
BQ108127|BQ108127.1 shrpID000306 LvG Litopenaeus vannamei cDNA, mRNA sequence.    44  3.1  1
L46781|L46781.1 Rattus norvegicus plasma membrane calcium ATPase isoform 3 gene, exon 12.    44  3.1  1
dna update 2003. 9.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54706) RecName: Full=Cofilin-1;  &D37980_1(D37980|pid:none) &D...   280   4e-74
(P37167) RecName: Full=Actophorin;  &M93361_1(M93361|pid:none)        130   6e-29
FM992692_38(FM992692|pid:none) Candida dubliniensis CD36 chromos...   125   2e-27
(Q96VU9) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   125   3e-27
AY428595_1(AY428595|pid:none) Helianthus annuus clone HA10-7 put...   123   8e-27
CP000501_299(CP000501|pid:none) Pichia stipitis CBS 6054 chromos...   120   9e-26
(Q9HF97) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   119   2e-25
(Q6CQ22) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   119   2e-25
CU928179_306(CU928179|pid:none) Zygosaccharomyces rouxii strain ...   116   1e-24
(Q03048) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   116   1e-24
protein update 2009. 6.19
PSORT 

 psg: 0.55  gvh: 0.34  alm: 0.58  top: 0.53  tms: 0.00  mit: 0.57  mip: 0.00
 nuc: 0.24  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 32.0 %: nuclear
	 12.0 %: cytoplasmic
	  4.0 %: endoplasmic reticulum

>> prediction for VFE845 is mit
5' end seq. ID VFE845F
5' end seq. 
>VFE845F.Seq
AACTTACTCAACTAAATAAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCT
CCAAACTGCGTTTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATC
TACAGAATCTCTGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGT
TCATTCGATGAATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGAC
TATCAATACAAAGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCA
GATACCGCCAACATTAAAAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAA
GCCTGTGTTGGTATCCAAGTTGAAATCCAAGGTACCGATGCCTCTGAAGTCAAAGATTCA
TGTTTCTATGAAAAATGTACCAAAATCTAAATAATTTGAAAAAATTTAAAAAAAAAA---
-------
Length of 5' end seq. 477
3' end seq. ID VFE845Z
3' end seq. 
>VFE845Z.Seq
----------AACTTACTCAACTAAATAAAAAACTATATATAAAAAATGTCTTCAGGTAT
TGCTTTAGCTCCAAACTGCGTTTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGG
TGGTATCATCTACAGAATCTCTGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACC
AGCTGGTTGTTCATTCGATGAATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGT
TGTCCTCGACTATCAATACAAAGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGC
CTGGTGCCCAGATACCGCCAACATTAAAAAGAAAATGATGGCCACCTCATCAAAAGACTC
TTTACGTAAAGCCTGTGTTGGTATCCAAGTTGAAATCCAAGGTACCGATGCCTNCTGAAG
TCAAAGATTCATGTTTCTATAAAAATGTACCAAAATCTAAATA
Length of 3' end seq. 453
Connected seq. ID VFE845P
Connected seq. 
>VFE845P.Seq
AACTTACTCAACTAAATAAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCT
CCAAACTGCGTTTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATC
TACAGAATCTCTGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGT
TCATTCGATGAATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGAC
TATCAATACAAAGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCA
GATACCGCCAACATTAAAAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAA
GCCTGTGTTGGTATCCAAGTTGAAATCCAAGGTACCGATGCCTCTGAAGTCAAAGATTCA
TGTTTCTATGAAAAATGTACCAAAATCTAAATAATTTGAAAAAATTTAAAAAAAAAA---
-------AACTTACTCAACTAAATAAAAAACTATATATAAAAAATGTCTTCAGGTATTGC
TTTAGCTCCAAACTGCGTTTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGG
TATCATCTACAGAATCTCTGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAGC
TGGTTGTTCATTCGATGAATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTGT
CCTCGACTATCAATACAAAGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTG
GTGCCCAGATACCGCCAACATTAAAAAGAAAATGATGGCCACCTCATCAAAAGACTCTTT
ACGTAAAGCCTGTGTTGGTATCCAAGTTGAAATCCAAGGTACCGATGCCTNCTGAAGTCA
AAGATTCATGTTTCTATAAAAATGTACCAAAATCTAAATA
Length of connected seq. 930
Full length Seq ID -
Full length Seq. -
Length of full length seq. -