>VFE720 (VFE720Q) /CSM/VF/VFE7-A/VFE720Q.Seq.d/
ACACACTACATAAACATATTTTTTTCTTATACATATAAACAAATAAATCAAATACCATGG
ATGAAAGTATTAAAAAGTTACCAGATTTAGCTATTGCTGATTTAATTTTCACTGTTAAAT
TAGAAGGTAAAACTGGTACTGAATCATATAATAAATTAATTAAAGAAATTGAAACACACA
AAATGTTACCATTATATAAAATACTTGTAGAACAATTAAAATGGACAGAAGATCAAGGAT
TAGTATCAAAATTAAAAGCTGAAAATGAAAGTGAATTAAAAGCATTAGATAATAAAATTA
CTGATAGTGTTGAAAATTTTGGTGAGTCTGAAATTAGAGAAGCATATTTAGCAAAATCTG
ATTTCTATTGTCGTATCGGTGATAAAGATACAGCAGTTGAAATGTATAGACAAACGTTTG
AAAAAACAGTACCATTGGGTCAAAAATTAGATATTGTTTTTACATTAATTAGAATGGGTA
TTTTTTGGATGGATCATGATATTGTAACAAGAAATTTAGAGAAAGCTCAATCATTAGTTG
AAGAAGGTGGTGATTGGGATAGAAAGAATCGTTTGAAAXXXXXXXXXX

tlhkhifflyi*TNKSNTMDESIKKLPDLAIADLIFTVKLEGKTGTESYNKLIKEIETHK
MLPLYKILVEQLKWTEDQGLVSKLKAENESELKALDNKITDSVENFGESEIREAYLAKSD
FYCRIGDKDTAVEMYRQTFEKTVPLGQKLDIVFTLIRMGIFWMDHDIVTRNLEKAQSLVE
EGGDWDRKNRL---

Frame A:
thyiniffsytykqinqipwmkvlksyqi*llli*fslln*kvklvlnhiin*lkklkht
kcyhyikyl*nn*ngqkikd*yqn*klkmkvn*kh*iikllivlkilvslklekhi*qnl
isivvsvikiqqlkcidkrlkkqyhwvkn*ilflh*lewvffgwimil*qei*rklnh*l
kkvvigieriv*---


Frame B:
htt*tyfflihink*ikyhg*ky*kvtrfsyc*fnfhc*irr*nwy*ii**in*rn*ntq
nvtii*ntcrtikmdrrsrisikiks*k*k*iksir**ny**c*kfw*v*n*rsifski*
fllsyr**ryss*nv*tnv*knstigskirycfyin*ngyfldgs*ycnkkfressiis*
rrw*lg*kesfe---


Frame C:
tlhkhifflyi*TNKSNTMDESIKKLPDLAIADLIFTVKLEGKTGTESYNKLIKEIETHK
MLPLYKILVEQLKWTEDQGLVSKLKAENESELKALDNKITDSVENFGESEIREAYLAKSD
FYCRIGDKDTAVEMYRQTFEKTVPLGQKLDIVFTLIRMGIFWMDHDIVTRNLEKAQSLVE
EGGDWDRKNRL---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFE720 (VFE720Q) /CSM/VF/VFE7-A/VFE720Q.Seq.d/                       1104   0.0
AHB478 (AHB478Q) /CSM/AH/AHB4-D/AHB478Q.Seq.d/                       1094   0.0
AFJ152 (AFJ152Q) /CSM/AF/AFJ1-C/AFJ152Q.Seq.d/                       1094   0.0
AFF282 (AFF282Q) /CSM/AF/AFF2-D/AFF282Q.Seq.d/                       1094   0.0
AFB302 (AFB302Q) /CSM/AF/AFB3-A/AFB302Q.Seq.d/                       1094   0.0
VFB468 (VFB468Q) /CSM/VF/VFB4-C/VFB468Q.Seq.d/                       1088   0.0
VFL342 (VFL342Q) /CSM/VF/VFL3-B/VFL342Q.Seq.d/                       1086   0.0
VFE365 (VFE365Q) /CSM/VF/VFE3-C/VFE365Q.Seq.d/                       1080   0.0
AFK561 (AFK561Q) /CSM/AF/AFK5-C/AFK561Q.Seq.d/                       1080   0.0
AFN846 (AFN846Q) /CSM/AF/AFN8-B/AFN846Q.Seq.d/                       1063   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AZ674420|AZ674420.1 ENTKF14TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    46  1e-05  2
AL627106|AL627106.8 Human DNA sequence from clone RP11-7K11 on chromosome 1.    42  6e-05  4
BQ605169|BQ605169.1 ar05-a10 Artemia EII Stage, Stratagene (cat. 200450) Artemia franciscana cDNA clone ar05-a10 5' similar to 26S proteasome regulatory complex subunit p42A, mRNA sequence.    48  0.005  2
AG261377|AG261377.1 Lotus corniculatus var. japonicus DNA, clone:LjT52o21_sfi.    52  0.008  1
AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    38  0.016  9
BI643417|BI643417.1 RS1_B03 Sugar beet root cDNA library (subtracted) Beta vulgaris cDNA 5', mRNA sequence.    50  0.031  1
BI643292|BI643292.1 RS3_C04 Sugar beet root cDNA library (subtracted) Beta vulgaris cDNA 5' similar to 26S proteasome regulatory particle-like (e-60), mRNA sequence.    50  0.031  1
BI643279|BI643279.1 RS3_E12 Sugar beet root cDNA library (subtracted) Beta vulgaris cDNA 5' similar to 26S proteasome regulatory particle-like (e-60), mRNA sequence.    50  0.031  1
CA835497|CA835497.1 MCS037F12_160502 Ice plant Lambda Uni-Zap XR expression library, 5 days 0.5 M NaCl treatment, Crassulacean acid metabolism, phase I (2 AM). Mesembryanthemum crystallinum cDNA clone MCS037F12 5, mRNA sequence.    50  0.031  1
AP004173|AP004173.1 Mycoplasma penetrans genomic DNA, complete sequence, section 4/5.    32  0.041  11

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55C75) RecName: Full=26S proteasome non-ATPase regulatory subu...   347   1e-94
BC064247_1(BC064247|pid:none) Xenopus tropicalis proteasome (pro...   183   3e-45
BC059159_1(BC059159|pid:none) Rattus norvegicus proteasome (pros...   182   4e-45
(Q3T0B2) RecName: Full=26S proteasome non-ATPase regulatory subu...   182   6e-45
(Q15008) RecName: Full=26S proteasome non-ATPase regulatory subu...   181   1e-44
BT075430_1(BT075430|pid:none) Osmerus mordax clone omor-eva-513-...   180   2e-44
BT043716_1(BT043716|pid:none) Salmo salar clone HM5_2909 proteas...   179   4e-44
BT073643_1(BT073643|pid:none) Oncorhynchus mykiss clone omyk-evn...   178   1e-43
BT082315_1(BT082315|pid:none) Anoplopoma fimbria clone afim-evh-...   176   4e-43
BT047707_1(BT047707|pid:none) Salmo salar clone ssal-eve-502-367...   174   1e-42

 psg: 0.75  gvh: 0.39  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 24.0 %: cytoskeletal
	 24.0 %: cytoplasmic
	 12.0 %: mitochondrial

>> prediction for VFE720 is nuc

>VFE720F.Seq
ACACACTACATAAACATATTTTTTTCTTATACATATAAACAAATAAATCAAATACCATGG
ATGAAAGTATTAAAAAGTTACCAGATTTAGCTATTGCTGATTTAATTTTCACTGTTAAAT
TAGAAGGTAAAACTGGTACTGAATCATATAATAAATTAATTAAAGAAATTGAAACACACA
AAATGTTACCATTATATAAAATACTTGTAGAACAATTAAAATGGACAGAAGATCAAGGAT
TAGTATCAAAATTAAAAGCTGAAAATGAAAGTGAATTAAAAGCATTAGATAATAAAATTA
CTGATAGTGTTGAAAATTTTGGTGAGTCTGAAATTAGAGAAGCATATTTAGCAAAATCTG
ATTTCTATTGTCGTATCGGTGATAAAGATACAGCAGTTGAAATGTATAGACAAACGTTTG
AAAAAACAGTACCATTGGGTCAAAAATTAGATATTGTTTTTACATTAATTAGAATGGGTA
TTTTTTGGATGGATCATGATATTGTAACAAGAAATTTAGAGAAAGCTCAATCATTAGTTG
AAGAAGGTGGTGATTGGGATAGAAAGAATCGTTTGAAA----------