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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM425
Library AF
(Link to library)
Clone ID AFM425
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM425P
(Link to Original site)
Representative DNA sequence 
>AFM425 (AFM425Q) /CSM/AF/AFM4-B/AFM425Q.Seq.d/
AAAAAAATAATGATCAGATCAGCAAGACTCATTTCAAATATTAAATTTGGACAAAAAAAT
ATTAGACAATTCTCAACCAATACAAATTGGTGGGCAAATGTTCAAAAGGGACCAGAAGAT
CCAATTTTAGGTGTTAGCATTGCTTACAATAAAGATACCTCACCATCAAAGATCAATTTA
GGTGTTGGTGCATACAGAGATGAAAATGGTAAACCATACGTATTGGATTGCGTAAAGAAA
GCAGACAAAAAGATCTATGAAGCCAACGTCGATCACGAATACGCACCAATTGTTGGTGTT
GCAGCATTCAATCAATTGGCAGCACAATTAGCATTGGGTGAAGAGTGTAAACATATTAAA
GAGAAACGTATTGCCACCGTTCAATCGATTTCAGGTACTGGTGCTTTAAGAATTGCCGCT
GATTTCTTTGCCCGTTTCCTCAAGGGTAAAACCGCATACGTACCAAACCCAACATGGGGT
AACCATAATGTTATTTTCAACGATGCAGGCATTCCAGTCAAATCCTACGXXXXXXXXXXG
AAGCCATGACAAAGGATATCGCTGCCGCTCCAGAGGGTTCAATCATTTTATTACATGCAT
GTGCTCACAATCCAACCGGTGTCGATCCAACCGCTGAGCAATGGAAAAAGATCTCTGAAA
TCTGTAAAGAGCGTGGTCATTTTGTTCTCTTTGATTTCGCTTATCAAGGTTTCGCATCTG
GTAGCCCAGAAAAGGATGCTGCCGCTGTCCGTATGTTTGTCGAAGATGGTCACAACATTG
CTCTCTGTCAATCATTTGCTAAAAACTTTGGTCTCTATGGTGAACGTATTGGTGCTTTCT
CCATTCTCACTGAAACTTCAGATCAAGCTCTCAACGTTGAATCTCAATTAAAGATCTTAA
TTCGTCCAATGTACTCTAATCCACCAGTTTATGGAGCTCGTCTCGTTCAAGCTATCCTCA
AAGATAAAGAATTAACCAATGAATGGAGATCCGAAGTTAAAGGTATGGCCGATCGTATCA
TTAACATGAGAGAACAATTAGTCAAATACCTTAAAAAACATGGTTCAACTCGTGATTGGT
CTCATATTACCACTCAAATTGGTATGTTTTGTTTCACTGGTTTAACTCCAGAACAAGTTG
ATCGTTTGGCAAATGAATATCACATCTATTTAACTCGTAATGGTCGTATCTCAATTGCTG
GTATAATTCAACAAATGTNAATATCTCGCTAAAGCAAGGCGCCGTACAAAAGA
sequence update 2009. 4. 3
Translated Amino Acid sequence 
KKIMIRSARLISNIKFGQKNIRQFSTNTNWWANVQKGPEDPILGVSIAYNKDTSPSKINL
GVGAYRDENGKPYVLDCVKKADKKIYEANVDHEYAPIVGVAAFNQLAAQLALGEECKHIK
EKRIATVQSISGTGALRIAADFFARFLKGKTAYVPNPTWGNHNVIFNDAGIPVKSY---

---EAMTKDIAAAPEGSIILLHACAHNPTGVDPTAEQWKKISEICKERGHFVLFDFAYQG
FASGSPEKDAAAVRMFVEDGHNIALCQSFAKNFGLYGERIGAFSILTETSDQALNVESQL
KILIRPMYSNPPVYGARLVQAILKDKELTNEWRSEVKGMADRIINMREQLVKYLKKHGST
RDWSHITTQIGMFCFTGLTPEQVDRLANEYHIYLTRNGRISIAGIIQQMXISR*skapyk
r
Translated Amino Acid sequence (All Frames) 
Frame A:
KKIMIRSARLISNIKFGQKNIRQFSTNTNWWANVQKGPEDPILGVSIAYNKDTSPSKINL
GVGAYRDENGKPYVLDCVKKADKKIYEANVDHEYAPIVGVAAFNQLAAQLALGEECKHIK
EKRIATVQSISGTGALRIAADFFARFLKGKTAYVPNPTWGNHNVIFNDAGIPVKSY---

---EAMTKDIAAAPEGSIILLHACAHNPTGVDPTAEQWKKISEICKERGHFVLFDFAYQG
FASGSPEKDAAAVRMFVEDGHNIALCQSFAKNFGLYGERIGAFSILTETSDQALNVESQL
KILIRPMYSNPPVYGARLVQAILKDKELTNEWRSEVKGMADRIINMREQLVKYLKKHGST
RDWSHITTQIGMFCFTGLTPEQVDRLANEYHIYLTRNGRISIAGIIQQMXISR*skapyk
r

Frame B:
kk**sdqqdsfqilnldkkildnsqpiqiggqmfkrdqkiqf*vlalltikiphhqrsi*
vlvhtemkmvnhtywia*rkqtkrsmkptsitnthqllvlqhsinwqhn*hwvksvnilk
rnvlppfnrfqvlvl*elplislpvssrvkphtyqtqhgvtimlfstmqafqsnpt---

---kp*qrislplqrvqsfyymhvltiqpvsiqplsngkrslksvksvvilfslislikv
shlvaqkrmlplsvclskmvttllsvnhllktlvsmvnvlvlspfslklqiklstlnln*
rs*fvqctlihqfmelvsfklsskikn*pmngdpklkvwpivslt*enn*sntlknmvql
viglilplklvcfvslv*lqnklivwqmnitsi*lvmvvsqllv*fnkcxylakarrrtk

Frame C:
knndqiskthfky*iwtkky*tilnqyklvgkcskgtrrsnfrc*hclq*ryltikdqfr
cwciqr*kw*tiriglrkesrqkdl*sqrrsrirtncwccsiqsigstisig*rv*ty*r
etychrsidfrywcfkncr*flcpfpqg*nrirtkpnmg*p*cyfqrcrhssqil---

---shdkgyrcrsrgfnhfitcmcsqsnrcrsnr*amekdl*nl*rawsfcsl*frlsrf
riw*prkgccrcpyvcrrwsqhcslsiic*klwslw*tywcflhsh*nfrsssqr*isik
dlnssnvl*stslwsssrssypqr*rinq*meirs*rygrsyh*hertisqip*ktwfns
*lvsyyhsnwyvlfhwfnsrts*sfgk*ishlfns*wsylncwynstnvnislkqgavqk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM425 (AFM425Q) /CSM/AF/AFM4-B/AFM425Q.Seq.d/                       2363   0.0
AFM850 (AFM850Q) /CSM/AF/AFM8-C/AFM850Q.Seq.d/                       1370   0.0
AFM514 (AFM514Q) /CSM/AF/AFM5-A/AFM514Q.Seq.d/                       1368   0.0
SFH219 (SFH219Q) /CSM/SF/SFH2-A/SFH219Q.Seq.d/                       1366   0.0
SFI739 (SFI739Q) /CSM/SF/SFI7-B/SFI739Q.Seq.d/                       1352   0.0
VFJ338 (VFJ338Q) /CSM/VF/VFJ3-B/VFJ338Q.Seq.d/                       1350   0.0
AHJ715 (AHJ715Q) /CSM/AH/AHJ7-A/AHJ715Q.Seq.d/                       1342   0.0
SFE417 (SFE417Q) /CSM/SF/SFE4-A/SFE417Q.Seq.d/                       1340   0.0
AFN637 (AFN637Q) /CSM/AF/AFN6-B/AFN637Q.Seq.d/                       1340   0.0
VFL403 (VFL403Q) /CSM/VF/VFL4-A/VFL403Q.Seq.d/                       1318   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ346841) Dictyostelium discoideum cDNA clone:dda25b08, 3' ...  1407   0.0    1
(BJ400838) Dictyostelium discoideum cDNA clone:dds15e06, 3' ...  1350   0.0    1
(BJ400155) Dictyostelium discoideum cDNA clone:dds9b06, 3' e...  1338   0.0    1
(BJ345650) Dictyostelium discoideum cDNA clone:dda28i10, 3' ...  1336   0.0    1
(BJ432675) Dictyostelium discoideum cDNA clone:ddv19l09, 3' ...  1316   0.0    2
(BJ347243) Dictyostelium discoideum cDNA clone:dda26d14, 3' ...  1316   0.0    2
(BJ347111) Dictyostelium discoideum cDNA clone:dda26k03, 3' ...  1316   0.0    2
(BJ347702) Dictyostelium discoideum cDNA clone:dda27k24, 3' ...  1302   0.0    1
(BJ353441) Dictyostelium discoideum cDNA clone:dda50n03, 3' ...  1292   0.0    2
(BJ401762) Dictyostelium discoideum cDNA clone:dds19g07, 3' ...  1267   0.0    1
dna update 2009. 4.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55F21) RecName: Full=Aspartate aminotransferase, mitochondrial...   451   0.0
(P00505) RecName: Full=Aspartate aminotransferase, mitochondrial...   299   e-122
(Q4R559) RecName: Full=Aspartate aminotransferase, mitochondrial...   300   e-121
CR860094_1(CR860094|pid:none) Pongo abelii mRNA; cDNA DKFZp469B0...   298   e-121
(P08907) RecName: Full=Aspartate aminotransferase, mitochondrial...   291   e-120
AK290847_1(AK290847|pid:none) Homo sapiens cDNA FLJ78439 complet...   295   e-120
U82470_1(U82470|pid:none) Mus musculus aspartate aminotransferas...   294   e-119
(P05202) RecName: Full=Aspartate aminotransferase, mitochondrial...   294   e-119
(P12344) RecName: Full=Aspartate aminotransferase, mitochondrial...   288   e-119
BC054684_1(BC054684|pid:none) Danio rerio glutamic-oxaloacetic t...   296   e-119
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM425 is

5' end seq. ID AFM425F
5' end seq. 
>AFM425F.Seq
AAAAAAATAATGATCAGATCAGCAAGACTCATTTCAAATATTAAATTTGGACAAAAAAAT
ATTAGACAATTCTCAACCAATACAAATTGGTGGGCAAATGTTCAAAAGGGACCAGAAGAT
CCAATTTTAGGTGTTAGCATTGCTTACAATAAAGATACCTCACCATCAAAGATCAATTTA
GGTGTTGGTGCATACAGAGATGAAAATGGTAAACCATACGTATTGGATTGCGTAAAGAAA
GCAGACAAAAAGATCTATGAAGCCAACGTCGATCACGAATACGCACCAATTGTTGGTGTT
GCAGCATTCAATCAATTGGCAGCACAATTAGCATTGGGTGAAGAGTGTAAACATATTAAA
GAGAAACGTATTGCCACCGTTCAATCGATTTCAGGTACTGGTGCTTTAAGAATTGCCGCT
GATTTCTTTGCCCGTTTCCTCAAGGGTAAAACCGCATACGTACCAAACCCAACATGGGGT
AACCATAATGTTATTTTCAACGATGCAGGCATTCCAGTCAAATCCTACGXXXXXXXXXX
Length of 5' end seq. 539
3' end seq. ID AFM425Z
3' end seq. 
>AFM425Z.Seq
XXXXXXXXXXGAAGCCATGACAAAGGATATCGCTGCCGCTCCAGAGGGTTCAATCATTTT
ATTACATGCATGTGCTCACAATCCAACCGGTGTCGATCCAACCGCTGAGCAATGGAAAAA
GATCTCTGAAATCTGTAAAGAGCGTGGTCATTTTGTTCTCTTTGATTTCGCTTATCAAGG
TTTCGCATCTGGTAGCCCAGAAAAGGATGCTGCCGCTGTCCGTATGTTTGTCGAAGATGG
TCACAACATTGCTCTCTGTCAATCATTTGCTAAAAACTTTGGTCTCTATGGTGAACGTAT
TGGTGCTTTCTCCATTCTCACTGAAACTTCAGATCAAGCTCTCAACGTTGAATCTCAATT
AAAGATCTTAATTCGTCCAATGTACTCTAATCCACCAGTTTATGGAGCTCGTCTCGTTCA
AGCTATCCTCAAAGATAAAGAATTAACCAATGAATGGAGATCCGAAGTTAAAGGTATGGC
CGATCGTATCATTAACATGAGAGAACAATTAGTCAAATACCTTAAAAAACATGGTTCAAC
TCGTGATTGGTCTCATATTACCACTCAAATTGGTATGTTTTGTTTCACTGGTTTAACTCC
AGAACAAGTTGATCGTTTGGCAAATGAATATCACATCTATTTAACTCGTAATGGTCGTAT
CTCAATTGCTGGTATAATTCAACAAATGTNAATATCTCGCTAAAGCAAGGCGCCGTACAA
AAGA
Length of 3' end seq. 724
Connected seq. ID AFM425P
Connected seq. 
>AFM425P.Seq
AAAAAAATAATGATCAGATCAGCAAGACTCATTTCAAATATTAAATTTGGACAAAAAAAT
ATTAGACAATTCTCAACCAATACAAATTGGTGGGCAAATGTTCAAAAGGGACCAGAAGAT
CCAATTTTAGGTGTTAGCATTGCTTACAATAAAGATACCTCACCATCAAAGATCAATTTA
GGTGTTGGTGCATACAGAGATGAAAATGGTAAACCATACGTATTGGATTGCGTAAAGAAA
GCAGACAAAAAGATCTATGAAGCCAACGTCGATCACGAATACGCACCAATTGTTGGTGTT
GCAGCATTCAATCAATTGGCAGCACAATTAGCATTGGGTGAAGAGTGTAAACATATTAAA
GAGAAACGTATTGCCACCGTTCAATCGATTTCAGGTACTGGTGCTTTAAGAATTGCCGCT
GATTTCTTTGCCCGTTTCCTCAAGGGTAAAACCGCATACGTACCAAACCCAACATGGGGT
AACCATAATGTTATTTTCAACGATGCAGGCATTCCAGTCAAATCCTACG----------G
AAGCCATGACAAAGGATATCGCTGCCGCTCCAGAGGGTTCAATCATTTTATTACATGCAT
GTGCTCACAATCCAACCGGTGTCGATCCAACCGCTGAGCAATGGAAAAAGATCTCTGAAA
TCTGTAAAGAGCGTGGTCATTTTGTTCTCTTTGATTTCGCTTATCAAGGTTTCGCATCTG
GTAGCCCAGAAAAGGATGCTGCCGCTGTCCGTATGTTTGTCGAAGATGGTCACAACATTG
CTCTCTGTCAATCATTTGCTAAAAACTTTGGTCTCTATGGTGAACGTATTGGTGCTTTCT
CCATTCTCACTGAAACTTCAGATCAAGCTCTCAACGTTGAATCTCAATTAAAGATCTTAA
TTCGTCCAATGTACTCTAATCCACCAGTTTATGGAGCTCGTCTCGTTCAAGCTATCCTCA
AAGATAAAGAATTAACCAATGAATGGAGATCCGAAGTTAAAGGTATGGCCGATCGTATCA
TTAACATGAGAGAACAATTAGTCAAATACCTTAAAAAACATGGTTCAACTCGTGATTGGT
CTCATATTACCACTCAAATTGGTATGTTTTGTTTCACTGGTTTAACTCCAGAACAAGTTG
ATCGTTTGGCAAATGAATATCACATCTATTTAACTCGTAATGGTCGTATCTCAATTGCTG
GTATAATTCAACAAATGTNAATATCTCGCTAAAGCAAGGCGCCGTACAAAAGA
Length of connected seq. 1243
Full length Seq ID -
Full length Seq. -
Length of full length seq. -