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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFE671
Library VF
(Link to library)
Clone ID VFE671
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VFE671P
(Link to Original site)
Representative DNA sequence 
>VFE671 (VFE671Q) /CSM/VF/VFE6-C/VFE671Q.Seq.d/
AATNATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATG
AGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTT
CATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCAT
AAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAA
AATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTC
CCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAA
AAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCA
TTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAG
AGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAAC
AAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACC
TACAAAGTACTCGTCGATAACAAXXXXXXXXXXTGACGTAAAGGAAATCGATGACCCAGA
AGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGT
CAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAA
TCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGA
ATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTT
TAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAA
CAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGT
TGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGA
GAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGA
AGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGACAAAAA
AGAAGAAGTCAAAAAATCAACCAAAAAAGTNATCAGACGAACTTTAAANAAATAAATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
nxhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWH
KEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQ
KVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEIN
KVETDQLTHQYTLVISPDNTYKVLVDN---

---DVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWK
AKKIPNPEYKGEWVHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSV
EEAKDFSEKTFVANQEAEKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEV
AEEDLVKTDDKKEEVKKSTKKVIRRTLXK*i
Translated Amino Acid sequence (All Frames) 
Frame A:
nxhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWH
KEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQ
KVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEIN
KVETDQLTHQYTLVISPDNTYKVLVDN---

---*rkgnr*prrc*tsrs*rypsfnr*srscqtrgle*rrrr*mgspnhrqsriqr*me
skenpksriqr*mgtpin**prir*rq*tlpl**srcyrfrimas*iwfhl*qydrhrlc
rrs*rfl*kdlccqprsrkenv*rprsrqs*rekes*rkiscrksc*krsrrs**rrrrs
r*rrfs*n**qkrrsqkinqksxqtnfkxin

Frame B:
xiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigi
kkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnk
klivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkkst
klkpin*lintlwsfhqitptkyssit---

---DVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWK
AKKIPNPEYKGEWVHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSV
EEAKDFSEKTFVANQEAEKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEV
AEEDLVKTDDKKEEVKKSTKKVIRRTLXK*i

Frame C:
xsyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*
rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tk
s*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqernqq
s*nrsinssihfghftr*hlqstrr*q---

---t*rksmtqkmlnqpvmtisqlqsliqklsnqrigmkkttvngkpqpspiqntkvngk
qrksqiqntkvngyth*litqntlktmnstslmisvlsvsnygklnlvpslti*ssptls
kklkislkrpllptkkpkrkclttskppklkrerkltkn*lpkkllkkkpkklmkkkkks
lkki*lklmtkkkksknqpkkxsdel*xnk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFE671 (VFE671Q) /CSM/VF/VFE6-C/VFE671Q.Seq.d/                       1953   0.0
VFM435 (VFM435Q) /CSM/VF/VFM4-B/VFM435Q.Seq.d/                       1027   0.0
VFM276 (VFM276Q) /CSM/VF/VFM2-D/VFM276Q.Seq.d/                       1027   0.0
VFH144 (VFH144Q) /CSM/VF/VFH1-B/VFH144Q.Seq.d/                       1027   0.0
VFH121 (VFH121Q) /CSM/VF/VFH1-A/VFH121Q.Seq.d/                       1027   0.0
VFD485 (VFD485Q) /CSM/VF/VFD4-D/VFD485Q.Seq.d/                       1027   0.0
VFD121 (VFD121Q) /CSM/VF/VFD1-A/VFD121Q.Seq.d/                       1027   0.0
VFA823 (VFA823Q) /CSM/VF/VFA8-A/VFA823Q.Seq.d/                       1027   0.0
VFA820 (VFA820Q) /CSM/VF/VFA8-A/VFA820Q.Seq.d/                       1027   0.0
SLE570 (SLE570Q) /CSM/SL/SLE5-C/SLE570Q.Seq.d/                       1027   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1019  0.0  5
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92  1e-23  3
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80  1e-17  3
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80  8e-11  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74  8e-11  2
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74  5e-09  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74  5e-09  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    74  5e-09  1
AI771812|AI771812.1 EST252912 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED38E1, mRNA sequence.    74  5e-09  1
BE435540|BE435540.1 EST406618 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG27F24, mRNA sequence.    74  5e-09  1
dna update 2003. 9.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   357   e-169
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   226   1e-98
AK318734_1(AK318734|pid:none) Arabidopsis thaliana AT1G56340 mRN...   226   3e-98
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   225   7e-98
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   223   2e-97
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   220   2e-97
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   223   2e-97
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   218   8e-97
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   223   8e-97
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   223   8e-97
protein update 2009. 6.19
PSORT 

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for VFE671 is exc
5' end seq. ID VFE671F
5' end seq. 
>VFE671F.Seq
AATNATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATG
AGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTT
CATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCAT
AAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAA
AATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTC
CCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAA
AAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCA
TTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAG
AGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAAC
AAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACC
TACAAAGTACTCGTCGATAACAA----------
Length of 5' end seq. 623
3' end seq. ID VFE671Z
3' end seq. 
>VFE671Z.Seq
----------TGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATG
ACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAG
AAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGA
AAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAATTGATA
ACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTT
TCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCGACTCTG
TCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGA
AAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAAT
TAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAG
TCGCTGAAGAAGATTTAGTTAAAACTGATGACAAAAAAGAAGAAGTCAAAAAATCAACCA
AAAAAGTNATCAGACGAACTTTAAANAAATAAATA
Length of 3' end seq. 625
Connected seq. ID VFE671P
Connected seq. 
>VFE671P.Seq
AATNATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATG
AGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTT
CATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCAT
AAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAA
AATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTC
CCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAA
AAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCA
TTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAG
AGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAAC
AAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACC
TACAAAGTACTCGTCGATAACAA----------TGACGTAAAGGAAATCGATGACCCAGA
AGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGT
CAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAA
TCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGA
ATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTT
TAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAA
CAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGT
TGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGA
GAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGA
AGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGACAAAAA
AGAAGAAGTCAAAAAATCAACCAAAAAAGTNATCAGACGAACTTTAAANAAATAAATA
Length of connected seq. 1248
Full length Seq ID -
Full length Seq. -
Length of full length seq. -