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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM422
Library AF
(Link to library)
Clone ID AFM422
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM422P
(Link to Original site)
Representative DNA sequence 
>AFM422 (AFM422Q) /CSM/AF/AFM4-A/AFM422Q.Seq.d/
TCACAAAAAGAAACAGCATGGTTAATTTTTCTTTCTCAAAAATTAAAAGAAAAAATGAAT
GGACCAAATGGTTTTAACCCTTTAGAACCATTTAATTCATTATTTGCAGAATTTAAAAAA
TACTCAACTCTTGCATGTTTAGGTTGTTTTAATATTGGTAAAACATTCTTTATCAATAAA
TACAATAATTCATATTTACCAAGTGGAACTCAATCACAAACCATTGGATTATCACTTTCA
ATTTGCCACAATAATGAAACAATTACAATTGATACAGCCGGTTCAAATACTTCATTAAAA
GTTGGTGAAAATAGATCAGAGGAACAATTGGCAAGAAAAGAATCCACTGAAATGTTTATT
CGTGATATGTCATTTTCATTAGCAAATACCATTGTTTATGTTTTAAATGAACTCACATGG
ACTGATCAAAGATTTATCTTGGCACTTCAATCAAAAATTCAATCATTAAGAACAGAACAA
AAAATAAACAAAACATTAATAATTGTTCATAACTATCCAAAAATCAATTCAATGGAGGAG
CTATTGGTTGAAATTAAAACATATATGGATCACCCATTTAATGGXXXXXXXXXXATCAGA
TCAACAAATAAAGAATATAATGACAAATTGGATTTTGATAAAGTTTTATTAAGTGGACTT
CAAAATAATATGGTTTCATATTGTAAATCACCATCAAAACTAAAGATTAGCGAATTTACA
AAAGATGATAAAATCATTGAAAATTGCAATAAAGAAAATGGTAAAATCTTATTTGACAAA
GAATCTGGTGAATTTTCAGTGCACTTAAAAGAACTTACAGAAATTTATGAAAAAGTTACT
CCACATAAAACTACAATTTCTCAAATACCAGTTTTCAAAATATATCCAGAAAATGATAGT
AATAGGGAAAATGAATTTAAATTAATAGATAACAAAGTTGAATTCATTGGTTTACAAATG
GTATTTTGGAATGGATTCAGACCAAACTATGATATTGTAAAAGTTGGTAGTGATTTACGT
TACCTTATTGAAGTTCCTCAAATGGTATTGGATGAAATTTTCCATGAAACAAAGTATGAA
AATACAAAATGGAATCTAATTGTCAAAGGTGTTAAAAAGTTACAATTTGACATGAAAAAT
TCAGAAAGTGGCTACCCAAGTGAAGAAACATCATTATACCTTTCAAATAATCAAAGACGT
GATGGTTCATTTGAACTCATACTCAACATTCCAAACAATTATCATCCAAAGAACCCAGAA
GTAGNANTAGAAAAAGGT
sequence update 2009. 4. 3
Translated Amino Acid sequence 
SQKETAWLIFLSQKLKEKMNGPNGFNPLEPFNSLFAEFKKYSTLACLGCFNIGKTFFINK
YNNSYLPSGTQSQTIGLSLSICHNNETITIDTAGSNTSLKVGENRSEEQLARKESTEMFI
RDMSFSLANTIVYVLNELTWTDQRFILALQSKIQSLRTEQKINKTLIIVHNYPKINSMEE
LLVEIKTYMDHPFN---

---IRSTNKEYNDKLDFDKVLLSGLQNNMVSYCKSPSKLKISEFTKDDKIIENCNKENGK
ILFDKESGEFSVHLKELTEIYEKVTPHKTTISQIPVFKIYPENDSNRENEFKLIDNKVEF
IGLQMVFWNGFRPNYDIVKVGSDLRYLIEVPQMVLDEIFHETKYENTKWNLIVKGVKKLQ
FDMKNSESGYPSEETSLYLSNNQRRDGSFELILNIPNNYHPKNPEVXXEKG
Translated Amino Acid sequence (All Frames) 
Frame A:
SQKETAWLIFLSQKLKEKMNGPNGFNPLEPFNSLFAEFKKYSTLACLGCFNIGKTFFINK
YNNSYLPSGTQSQTIGLSLSICHNNETITIDTAGSNTSLKVGENRSEEQLARKESTEMFI
RDMSFSLANTIVYVLNELTWTDQRFILALQSKIQSLRTEQKINKTLIIVHNYPKINSMEE
LLVEIKTYMDHPFN---

---IRSTNKEYNDKLDFDKVLLSGLQNNMVSYCKSPSKLKISEFTKDDKIIENCNKENGK
ILFDKESGEFSVHLKELTEIYEKVTPHKTTISQIPVFKIYPENDSNRENEFKLIDNKVEF
IGLQMVFWNGFRPNYDIVKVGSDLRYLIEVPQMVLDEIFHETKYENTKWNLIVKGVKKLQ
FDMKNSESGYPSEETSLYLSNNQRRDGSFELILNIPNNYHPKNPEVXXEKG

Frame B:
hkkkqhg*ffflkn*kkk*mdqmvltl*nhlihylqnlkntqllhv*vvlilvkhslsin
tiihiyqvelnhkpldyhfqfatimkqlqliqpvqilh*klvkidqrnnwqeknplkclf
vichfh*qiplfmf*mnshglikdlswhfnqkfnh*eqnkk*tkh**lfitiqksiqwrs
ywlklkhiwithlm---

---sdqqiknimtnwilikfy*vdfkiiwfhivnhhqn*rlanlqkmikslkiaikkmvk
syltknlvnfqct*knlqkfmkkllhiklqflkyqfskyiqkmivigkmnln**itklns
lvykwyfgmdsdqtmil*klvviyvtllkflkwywmkfsmkqsmkiqngi*lskvlksyn
lt*kiqkvatqvkkhhytfqiikdvmvhlnsystfqtiiiqrtqk*x*kk

Frame C:
tkrnsmvnfsfskikrknewtkwf*pfrti*fiicri*kilnscmfrlf*yw*nilyq*i
q*fiftkwnsitnhwiitfnlpq**nnyn*ysrfkyfiksw*k*irgtigkkrih*nvys
*yvifiskyhclcfk*thmd*skiylgtsiknsiiknrtknkqninncs*lsknqfngga
ig*n*niygspi*w---

---qink*ri**qigf**sfikwtsk*ygfil*itiktkd*riykr**nh*klq*rkw*n
li*qriw*ifsalkrtyrnl*ksyst*nynfsntsfqnisrk****gk*i*inr*qs*ih
wftngilewiqtkl*ycksw**ftlpy*sssngig*nfp*nkv*kykmesncqrc*kvti
*hekfrkwlpk*rniiipfk*skt*wfi*thtqhskqlsskeprsxxrkr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM422 (AFM422Q) /CSM/AF/AFM4-A/AFM422Q.Seq.d/                       2353   0.0
SFE471 (SFE471Q) /CSM/SF/SFE4-C/SFE471Q.Seq.d/                       1328   0.0
AFN817 (AFN817Q) /CSM/AF/AFN8-A/AFN817Q.Seq.d/                       1070   0.0
AFE688 (AFE688Q) /CSM/AF/AFE6-D/AFE688Q.Seq.d/                       1055   0.0
AFO508 (AFO508Q) /CSM/AF/AFO5-A/AFO508Q.Seq.d/                       1053   0.0
AFM284 (AFM284Q) /CSM/AF/AFM2-D/AFM284Q.Seq.d/                       1041   0.0
AFI319 (AFI319Q) /CSM/AF/AFI3-A/AFI319Q.Seq.d/                       1017   0.0
SLB350 (SLB350Q) /CSM/SL/SLB3-C/SLB350Q.Seq.d/                        912   0.0
AFC213 (AFC213Q) /CSM/AF/AFC2-A/AFC213Q.Seq.d/                        906   0.0
SSC196 (SSC196Q) /CSM/SS/SSC1-D/SSC196Q.Seq.d/                        250   5e-65
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ346817) Dictyostelium discoideum cDNA clone:dda25l06, 3' ...  1344   0.0    1
(BJ400307) Dictyostelium discoideum cDNA clone:dds9n18, 3' e...  1328   0.0    1
(BJ329488) Dictyostelium discoideum cDNA clone:dda25l06, 5' ...  1045   0.0    2
(BJ345535) Dictyostelium discoideum cDNA clone:dda28b06, 3' ...   952   0.0    2
(BJ339918) Dictyostelium discoideum cDNA clone:dda10o22, 3' ...  1055   0.0    1
(BJ346203) Dictyostelium discoideum cDNA clone:dda30o01, 3' ...  1053   0.0    1
(BJ347009) Dictyostelium discoideum cDNA clone:dda25g22, 3' ...  1041   0.0    1
(BJ343754) Dictyostelium discoideum cDNA clone:dda17f05, 3' ...  1013   0.0    1
(AU033715) Dictyostelium discoideum slug cDNA, clone SLB350.      912   0.0    1
(BJ341071) Dictyostelium discoideum cDNA clone:dda5i04, 3' e...   906   0.0    1
dna update 2009. 4.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54QE8) RecName: Full=Heat shock protein DDB_G0283913;               311   3e-83
AC116979_66(AC116979|pid:none) Dictyostelium discoideum chromoso...    48   8e-04
AP008971_512(AP008971|pid:none) Finegoldia magna ATCC 29328 DNA,...    44   0.009
AC116979_67(AC116979|pid:none) Dictyostelium discoideum chromoso...    42   0.034
T28676(T28676;A45521)rhoptry protein - Plasmodium yoelii (fragme...    42   0.045
AL035475_18(AL035475|pid:none) Plasmodium falciparum MAL4P2.  &A...    42   0.045
AM406671_1226(AM406671|pid:none) Lactococcus lactis subsp. cremo...    41   0.099
CP000123_39(CP000123|pid:none) Mycoplasma capricolum subsp. capr...    40   0.13
CP000312_335(CP000312|pid:none) Clostridium perfringens SM101, c...    40   0.22
AJ489165_1(AJ489165|pid:none) Plasmodium yoelii yoelii partial m...    39   0.38
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM422 is

5' end seq. ID AFM422F
5' end seq. 
>AFM422F.Seq
TCACAAAAAGAAACAGCATGGTTAATTTTTCTTTCTCAAAAATTAAAAGAAAAAATGAAT
GGACCAAATGGTTTTAACCCTTTAGAACCATTTAATTCATTATTTGCAGAATTTAAAAAA
TACTCAACTCTTGCATGTTTAGGTTGTTTTAATATTGGTAAAACATTCTTTATCAATAAA
TACAATAATTCATATTTACCAAGTGGAACTCAATCACAAACCATTGGATTATCACTTTCA
ATTTGCCACAATAATGAAACAATTACAATTGATACAGCCGGTTCAAATACTTCATTAAAA
GTTGGTGAAAATAGATCAGAGGAACAATTGGCAAGAAAAGAATCCACTGAAATGTTTATT
CGTGATATGTCATTTTCATTAGCAAATACCATTGTTTATGTTTTAAATGAACTCACATGG
ACTGATCAAAGATTTATCTTGGCACTTCAATCAAAAATTCAATCATTAAGAACAGAACAA
AAAATAAACAAAACATTAATAATTGTTCATAACTATCCAAAAATCAATTCAATGGAGGAG
CTATTGGTTGAAATTAAAACATATATGGATCACCCATTTAATGGXXXXXXXXXX
Length of 5' end seq. 594
3' end seq. ID AFM422Z
3' end seq. 
>AFM422Z.Seq
XXXXXXXXXXATCAGATCAACAAATAAAGAATATAATGACAAATTGGATTTTGATAAAGT
TTTATTAAGTGGACTTCAAAATAATATGGTTTCATATTGTAAATCACCATCAAAACTAAA
GATTAGCGAATTTACAAAAGATGATAAAATCATTGAAAATTGCAATAAAGAAAATGGTAA
AATCTTATTTGACAAAGAATCTGGTGAATTTTCAGTGCACTTAAAAGAACTTACAGAAAT
TTATGAAAAAGTTACTCCACATAAAACTACAATTTCTCAAATACCAGTTTTCAAAATATA
TCCAGAAAATGATAGTAATAGGGAAAATGAATTTAAATTAATAGATAACAAAGTTGAATT
CATTGGTTTACAAATGGTATTTTGGAATGGATTCAGACCAAACTATGATATTGTAAAAGT
TGGTAGTGATTTACGTTACCTTATTGAAGTTCCTCAAATGGTATTGGATGAAATTTTCCA
TGAAACAAAGTATGAAAATACAAAATGGAATCTAATTGTCAAAGGTGTTAAAAAGTTACA
ATTTGACATGAAAAATTCAGAAAGTGGCTACCCAAGTGAAGAAACATCATTATACCTTTC
AAATAATCAAAGACGTGATGGTTCATTTGAACTCATACTCAACATTCCAAACAATTATCA
TCCAAAGAACCCAGAAGTAGNANTAGAAAAAGGT
Length of 3' end seq. 694
Connected seq. ID AFM422P
Connected seq. 
>AFM422P.Seq
TCACAAAAAGAAACAGCATGGTTAATTTTTCTTTCTCAAAAATTAAAAGAAAAAATGAAT
GGACCAAATGGTTTTAACCCTTTAGAACCATTTAATTCATTATTTGCAGAATTTAAAAAA
TACTCAACTCTTGCATGTTTAGGTTGTTTTAATATTGGTAAAACATTCTTTATCAATAAA
TACAATAATTCATATTTACCAAGTGGAACTCAATCACAAACCATTGGATTATCACTTTCA
ATTTGCCACAATAATGAAACAATTACAATTGATACAGCCGGTTCAAATACTTCATTAAAA
GTTGGTGAAAATAGATCAGAGGAACAATTGGCAAGAAAAGAATCCACTGAAATGTTTATT
CGTGATATGTCATTTTCATTAGCAAATACCATTGTTTATGTTTTAAATGAACTCACATGG
ACTGATCAAAGATTTATCTTGGCACTTCAATCAAAAATTCAATCATTAAGAACAGAACAA
AAAATAAACAAAACATTAATAATTGTTCATAACTATCCAAAAATCAATTCAATGGAGGAG
CTATTGGTTGAAATTAAAACATATATGGATCACCCATTTAATGG----------ATCAGA
TCAACAAATAAAGAATATAATGACAAATTGGATTTTGATAAAGTTTTATTAAGTGGACTT
CAAAATAATATGGTTTCATATTGTAAATCACCATCAAAACTAAAGATTAGCGAATTTACA
AAAGATGATAAAATCATTGAAAATTGCAATAAAGAAAATGGTAAAATCTTATTTGACAAA
GAATCTGGTGAATTTTCAGTGCACTTAAAAGAACTTACAGAAATTTATGAAAAAGTTACT
CCACATAAAACTACAATTTCTCAAATACCAGTTTTCAAAATATATCCAGAAAATGATAGT
AATAGGGAAAATGAATTTAAATTAATAGATAACAAAGTTGAATTCATTGGTTTACAAATG
GTATTTTGGAATGGATTCAGACCAAACTATGATATTGTAAAAGTTGGTAGTGATTTACGT
TACCTTATTGAAGTTCCTCAAATGGTATTGGATGAAATTTTCCATGAAACAAAGTATGAA
AATACAAAATGGAATCTAATTGTCAAAGGTGTTAAAAAGTTACAATTTGACATGAAAAAT
TCAGAAAGTGGCTACCCAAGTGAAGAAACATCATTATACCTTTCAAATAATCAAAGACGT
GATGGTTCATTTGAACTCATACTCAACATTCCAAACAATTATCATCCAAAGAACCCAGAA
GTAGNANTAGAAAAAGGT
Length of connected seq. 1268
Full length Seq ID -
Full length Seq. -
Length of full length seq. -