>VFE666 (VFE666Q) /CSM/VF/VFE6-C/VFE666Q.Seq.d/
ATTTTTTTAAAGTTTTACATATATATAAACAATCAATTTAAAAAATGATGAAATTTTTAA
TTGTTTTAATTTTAGCATTATTCGCTTTATCAAGTGTTAATGCAAGCAGCGCTATTGAAT
GCGAACTTTGTGAATATGCTGCCAATTATGGTGAATTGCTCATTCAATCAAATGCCACCG
AAACTGAAATTATCGATAAAGTTGAAAACTTTTGCAAAGTTATCCCATCTAGTTTCCAAG
CCACTTGTGATAGTTTAATCGCAGACTATGGTAAACAACTCATTCAAATGATTGTCAACA
AAGAATCACCAAGCACTTTATGCGCTCAAATTGATATGTGTGCATCATCAACTGAAGCCG
TCCAAGGTATTTTAGAGTGTGACATTTGCCAATTCATCGTXXXXXXXXXX

ffkvlhiykqsi*KMMKFLIVLILALFALSSVNASSAIECELCEYAANYGELLIQSNATE
TEIIDKVENFCKVIPSSFQATCDSLIADYGKQLIQMIVNKESPSTLCAQIDMCASSTEAV
QGILECDICQFI---

Frame A:
iflkfyiyinnqfkk**nf*lf*f*hyslyqvlmqaallnanfvnmlpimvncsfnqmpp
klklsiklktfaklshlvskplviv*sqtmvnnsfk*lstknhqalyalklicvhhqlkp
skvf*svtfanss---


Frame B:
ff*sftyi*tinlkndeifncfnfsiirfikc*ckqry*mrtl*iccqlw*iahsikchr
n*nyr*s*kllqsypi*fpshl**fnrrlw*tthsndcqqritkhfmrsn*yvciin*sr
pryfrv*hlpih---


Frame C:
ffkvlhiykqsi*KMMKFLIVLILALFALSSVNASSAIECELCEYAANYGELLIQSNATE
TEIIDKVENFCKVIPSSFQATCDSLIADYGKQLIQMIVNKESPSTLCAQIDMCASSTEAV
QGILECDICQFI---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFE666 (VFE666Q) /CSM/VF/VFE6-C/VFE666Q.Seq.d/                        777   0.0
VFO870 (VFO870Q) /CSM/VF/VFO8-C/VFO870Q.Seq.d/                        761   0.0
VFM374 (VFM374Q) /CSM/VF/VFM3-D/VFM374Q.Seq.d/                        761   0.0
VFL608 (VFL608Q) /CSM/VF/VFL6-A/VFL608Q.Seq.d/                        761   0.0
VFL571 (VFL571Q) /CSM/VF/VFL5-C/VFL571Q.Seq.d/                        761   0.0
VFH688 (VFH688Q) /CSM/VF/VFH6-D/VFH688Q.Seq.d/                        761   0.0
VFF888 (VFF888Q) /CSM/VF/VFF8-D/VFF888Q.Seq.d/                        761   0.0
VFF843 (VFF843Q) /CSM/VF/VFF8-B/VFF843Q.Seq.d/                        761   0.0
VFF267 (VFF267Q) /CSM/VF/VFF2-C/VFF267Q.Seq.d/                        761   0.0
VFN547 (VFN547Q) /CSM/VF/VFN5-B/VFN547Q.Seq.d/                        755   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AU206530|AU206530.1 Caenorhabditis elegans cDNA clone:yk877f01 : 5' end, single read.   230  1e-56  1
AF175768|AF175768.1 Dictyostelium discoideum SAPLIP C protein mRNA, complete cds.    84  1e-13  2
AE001438|AE001438.3 Clostridium acetobutylicum megaplasmid pSOL1, complete sequence.    50  0.003  2
AC125718|AC125718.5 Rattus norvegicus clone CH230-24O15, WORKING DRAFT SEQUENCE, 3 unordered pieces.    48  0.084  1
AL731894|AL731894.5 Human DNA sequence from clone RP11-386G5 on chromosome 6.    38  0.28  4
BZ016954|BZ016954.1 oeh10e08.g1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    46  0.33  1
AI647659|AI647659.1 uk37e01.x1 Sugano mouse kidney mkia Mus musculus cDNA clone IMAGE:1971192 3', mRNA sequence.    46  0.33  1
AC124516|AC124516.2 Mus musculus chromosome UNK clone RP23-387C9, WORKING DRAFT SEQUENCE, 15 unordered pieces.    46  0.33  1
AF267217|AF267217.1 Candidatus Carsonella ruddii natural-host Pachypsylla celtidis ATP synthase alpha subunit (atpA) gene, partial cds; ATP synthase gamma subunit (atpG) gene, complete cds; and ATP synthase beta subunit (atpD) gene, partial cds.    46  0.33  1
BZ452600|BZ452600.1 BONEW76TR BO_1.6_2_KB_tot Brassica oleracea genomic clone BONEW76, DNA sequence.    46  0.33  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U66367_1(U66367|pid:none) Dictyostelium discoideum SapA (sapA) m...    74   1e-12
BT045905_1(BT045905|pid:none) Salmo salar clone ssal-rgf-536-147...    72   8e-12
CR388420_1(CR388420|pid:none) Zebrafish DNA sequence from clone ...    70   2e-11
BC056717_1(BC056717|pid:none) Danio rerio prosaposin, mRNA (cDNA...    70   2e-11
AF276996_1(AF276996|pid:none) Danio rerio lysosomal cofactor/neu...    70   2e-11
BT045327_1(BT045327|pid:none) Salmo salar clone ssal-rgf-518-210...    69   5e-11
BT058972_1(BT058972|pid:none) Salmo salar clone ssal-rgf-517-167...    69   5e-11
AC115608_3(AC115608|pid:none) Dictyostelium discoideum chromosom...    68   9e-11
AF108656_1(AF108656|pid:none) Gallus gallus prosaposin mRNA, com...    68   1e-10
(O13035) RecName: Full=Proactivator polypeptide; Contains:   Rec...    68   1e-10

 psg: 0.99  gvh: 0.88  alm: 0.48  top: 0.07  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: endoplasmic reticulum
	  8.0 %: vacuolar
	  4.0 %: nuclear

>> prediction for VFE666 is exc

>VFE666F.Seq
ATTTTTTTAAAGTTTTACATATATATAAACAATCAATTTAAAAAATGATGAAATTTTTAA
TTGTTTTAATTTTAGCATTATTCGCTTTATCAAGTGTTAATGCAAGCAGCGCTATTGAAT
GCGAACTTTGTGAATATGCTGCCAATTATGGTGAATTGCTCATTCAATCAAATGCCACCG
AAACTGAAATTATCGATAAAGTTGAAAACTTTTGCAAAGTTATCCCATCTAGTTTCCAAG
CCACTTGTGATAGTTTAATCGCAGACTATGGTAAACAACTCATTCAAATGATTGTCAACA
AAGAATCACCAAGCACTTTATGCGCTCAAATTGATATGTGTGCATCATCAACTGAAGCCG
TCCAAGGTATTTTAGAGTGTGACATTTGCCAATTCATCGT----------