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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFE631
Library VF
(Link to library)
Clone ID VFE631
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16272-1
Original site URL
Representative seq. ID VFE631P
(Link to Original site)
Representative DNA sequence 
>VFE631 (VFE631Q) /CSM/VF/VFE6-B/VFE631Q.Seq.d/
ACTTCTAAACAACAATAACAATATGAGTCATCGTAAATTTGAAGCTCCACGTCACGGTAA
TCTCGGTTTCAGACCAAGAAAGAGAGCCGCCAGACATCAAGGTAAAGTCAAGTCATTCCC
AAAGGATGACCGTACCCAAAAAGTCCACTTAACTGCATTCATGGGTTACAAAGCTGGTAT
GACTCACGTTGTCAGAGATTTAGAAAAACCAGGTTCAAAGATGCACAAAAAAGAAATCGT
TGAAGCCGTCACCATCATTGAATGTCCACCAATGTACATTGTTGGTTTAGTTGGTTATGT
TGAAACCGCTCAAGGTCTCAAAACCTACAAAACCGTCTGGGCTCAACATTTAAGTGATAA
CTTCCGTAGGAGATTATACAAAAAACTGGTACAAATCAAAATCAAAGAAAGCTTTCACCX
XXXXXXXXXANCNAATCTNANCCGGNAAGACNGAATTCGATTTAACTAAAANGNCTATCA
ATCCAATGGGTGGTTTCGCTNAAATACGGTATGGTTAAACATGAATTCTTAATGCTTAAA
GGTTGTGTTGCTGGTCCAAGAAAGAGAGCCTTAACCTTAAGAAAGAGTATCACCACTCAA
ACTGGTCGTGCTGCCCTCGAGAAAATCACTCTCAAATTCATCGATACCTCCAGCAAGTTT
GGTCACGGTCTTCATCAAACCGCTGAAGATAAGACTAAATATTTCGGCGTCAAGAAATCA
AG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LLNNNNNMSHRKFEAPRHGNLGFRPRKRAARHQGKVKSFPKDDRTQKVHLTAFMGYKAGM
THVVRDLEKPGSKMHKKEIVEAVTIIECPPMYIVGLVGYVETAQGLKTYKTVWAQHLSDN
FRRRLYKKLVQIKIKESFH---

---XIXXGKTEFDLTKXXINPMGGFAXIRYG*t*ilna*rlccwskkeslnlkkeyhhsn
wsccprenhsqihrylqqvwsrsssnr*r*d*ifrrqeik
Translated Amino Acid sequence (All Frames) 
Frame A:
tskqq*qyess*i*sstsr*srfqtkkesrqtsr*sqvipkg*pypksplncihglqswy
dsrcqrfrktrfkdaqkrnr*srhhh*mstnvhcwfswlc*nrsrsqnlqnrlgstfk**
lp*eiiqktgtnqnqrkls---

---xxsxpxrxnsi*lkxlsiqwvvslkygmvkheflmlkgcvagprkraltlrksittq
tgraalekitlkfidtsskfghglhqtaedktkyfgvkks

Frame B:
LLNNNNNMSHRKFEAPRHGNLGFRPRKRAARHQGKVKSFPKDDRTQKVHLTAFMGYKAGM
THVVRDLEKPGSKMHKKEIVEAVTIIECPPMYIVGLVGYVETAQGLKTYKTVWAQHLSDN
FRRRLYKKLVQIKIKESFH---

---xnlxrxdxirfn*xxyqsngwfrxntvwlnmns*clkvvllvqerep*p*ervsplk
lvvlpsrkslsnssippaslvtvfikplkirlnisasrnq

Frame C:
f*ttiti*vivnlklhvtvisvsdqereppdikvksshsqrmtvpkkst*lhswvtklv*
ltlsei*knqvqrctkkkslkpspslnvhqctllv*lvmlkplkvskptkpsglni*vit
svgdytknwykskskkaft---

---XIXXGKTEFDLTKXXINPMGGFAXIRYG*t*ilna*rlccwskkeslnlkkeyhhsn
wsccprenhsqihrylqqvwsrsssnr*r*d*ifrrqeik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFE631 (VFE631Q) /CSM/VF/VFE6-B/VFE631Q.Seq.d/                       1318   0.0
VHA485 (VHA485Q) /CSM/VH/VHA4-D/VHA485Q.Seq.d/                        807   0.0
VFO878 (VFO878Q) /CSM/VF/VFO8-D/VFO878Q.Seq.d/                        807   0.0
VFO705 (VFO705Q) /CSM/VF/VFO7-A/VFO705Q.Seq.d/                        807   0.0
VFO454 (VFO454Q) /CSM/VF/VFO4-C/VFO454Q.Seq.d/                        807   0.0
VFO195 (VFO195Q) /CSM/VF/VFO1-D/VFO195Q.Seq.d/                        807   0.0
VFO193 (VFO193Q) /CSM/VF/VFO1-D/VFO193Q.Seq.d/                        807   0.0
VFN841 (VFN841Q) /CSM/VF/VFN8-B/VFN841Q.Seq.d/                        807   0.0
VFN362 (VFN362Q) /CSM/VF/VFN3-C/VFN362Q.Seq.d/                        807   0.0
VFN190 (VFN190Q) /CSM/VF/VFN1-D/VFN190Q.Seq.d/                        807   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

L08391|L08391.1 Dictyostelium discoideum ribosomal protein (L3) gene, complete cds.   704  0.0  6
AX489150|AX489150.1 Sequence 6450 from Patent WO02053728.         42  3e-19  6
J01351|J01351.1 S.cerevisiae ribosomal protein L3 (tcm1) gene.    46  3e-11  4
AL398773|AL398773.1 T7 end of clone AS0AA011B09 of library AS0AA from strain CLIB 533 of Saccharomyces bayanus.    46  3e-11  4
BI419178|BI419178.1 LjNEST29c8r Lotus japonicus nodule library 5 and 7 week-old Lotus japonicus cDNA 5', mRNA sequence.    62  3e-11  2
AV429051|AV429051.1 Lotus japonicus cDNA clone:MWM118e09_r, 5' end.    62  4e-11  2
AV410149|AV410149.1 Lotus japonicus cDNA clone:MWL068f12_r, 5' end.    62  5e-11  2
AV413065|AV413065.1 Lotus japonicus cDNA clone:MWM227f10_r, 5' end.    62  5e-11  2
BG662092|BG662092.1 Ljirnpest38-108-g6 Ljirnp Lambda HybriZap two-hybrid library Lotus japonicus cDNA clone LP108-38-g6 5' similar to homolog of 60S ribosomal protein L3, mRNA sequence.    62  5e-11  2
BI419201|BI419201.1 LjNEST29f10r Lotus japonicus nodule library 5 and 7 week-old Lotus japonicus cDNA 5', mRNA sequence.    62  5e-11  2
dna update 2003. 9.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34113) RecName: Full=60S ribosomal protein L3;  &JC4254(JC4254...   255   8e-67
AK151084_1(AK151084|pid:none) Mus musculus bone marrow macrophag...   193   4e-48
AK151426_1(AK151426|pid:none) Mus musculus bone marrow macrophag...   193   4e-48
AK151142_1(AK151142|pid:none) Mus musculus bone marrow macrophag...   193   4e-48
AJ238851_1(AJ238851|pid:none) Bos taurus rpl3 gene for ribosomal...   193   4e-48
(P39872) RecName: Full=60S ribosomal protein L3;  &BC102253_1(BC...   193   4e-48
AK151057_1(AK151057|pid:none) Mus musculus bone marrow macrophag...   193   4e-48
CR926481_1(CR926481|pid:none) Pongo abelii mRNA; cDNA DKFZp468G0...   193   5e-48
BC008492_1(BC008492|pid:none) Homo sapiens ribosomal protein L3,...   193   5e-48
(P39023) RecName: Full=60S ribosomal protein L3; AltName: Full=H...   193   5e-48
protein update 2009. 6.19
PSORT 

 psg: 0.48  gvh: 0.21  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.36  mip: 0.06
 nuc: 0.18  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: nuclear
	 20.0 %: cytoplasmic
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for VFE631 is nuc
5' end seq. ID VFE631F
5' end seq. 
>VFE631F.Seq
ACTTCTAAACAACAATAACAATATGAGTCATCGTAAATTTGAAGCTCCACGTCACGGTAA
TCTCGGTTTCAGACCAAGAAAGAGAGCCGCCAGACATCAAGGTAAAGTCAAGTCATTCCC
AAAGGATGACCGTACCCAAAAAGTCCACTTAACTGCATTCATGGGTTACAAAGCTGGTAT
GACTCACGTTGTCAGAGATTTAGAAAAACCAGGTTCAAAGATGCACAAAAAAGAAATCGT
TGAAGCCGTCACCATCATTGAATGTCCACCAATGTACATTGTTGGTTTAGTTGGTTATGT
TGAAACCGCTCAAGGTCTCAAAACCTACAAAACCGTCTGGGCTCAACATTTAAGTGATAA
CTTCCGTAGGAGATTATACAAAAAACTGGTACAAATCAAAATCAAAGAAAGCTTTCACC-
---------
Length of 5' end seq. 419
3' end seq. ID VFE631Z
3' end seq. 
>VFE631Z.Seq
----------ANCNAATCTNANCCGGNAAGACNGAATTCGATTTAACTAAAANGNCTATC
AATCCAATGGGTGGTTTCGCTNAAATACGGTATGGTTAAACATGAATTCTTAATGCTTAA
AGGTTGTGTTGCTGGTCCAAGAAAGAGAGCCTTAACCTTAAGAAAGAGTATCACCACTCA
AACTGGTCGTGCTGCCCTCGAGAAAATCACTCTCAAATTCATCGATACCTCCAGCAAGTT
TGGTCACGGTCTTCATCAAACCGCTGAAGATAAGACTAAATATTTCGGCGTCAAGAAATC
AAG
Length of 3' end seq. 293
Connected seq. ID VFE631P
Connected seq. 
>VFE631P.Seq
ACTTCTAAACAACAATAACAATATGAGTCATCGTAAATTTGAAGCTCCACGTCACGGTAA
TCTCGGTTTCAGACCAAGAAAGAGAGCCGCCAGACATCAAGGTAAAGTCAAGTCATTCCC
AAAGGATGACCGTACCCAAAAAGTCCACTTAACTGCATTCATGGGTTACAAAGCTGGTAT
GACTCACGTTGTCAGAGATTTAGAAAAACCAGGTTCAAAGATGCACAAAAAAGAAATCGT
TGAAGCCGTCACCATCATTGAATGTCCACCAATGTACATTGTTGGTTTAGTTGGTTATGT
TGAAACCGCTCAAGGTCTCAAAACCTACAAAACCGTCTGGGCTCAACATTTAAGTGATAA
CTTCCGTAGGAGATTATACAAAAAACTGGTACAAATCAAAATCAAAGAAAGCTTTCACC-
---------ANCNAATCTNANCCGGNAAGACNGAATTCGATTTAACTAAAANGNCTATCA
ATCCAATGGGTGGTTTCGCTNAAATACGGTATGGTTAAACATGAATTCTTAATGCTTAAA
GGTTGTGTTGCTGGTCCAAGAAAGAGAGCCTTAACCTTAAGAAAGAGTATCACCACTCAA
ACTGGTCGTGCTGCCCTCGAGAAAATCACTCTCAAATTCATCGATACCTCCAGCAAGTTT
GGTCACGGTCTTCATCAAACCGCTGAAGATAAGACTAAATATTTCGGCGTCAAGAAATCA
AG
Length of connected seq. 712
Full length Seq ID -
Full length Seq. -
Length of full length seq. -