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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFE616
Library VF
(Link to library)
Clone ID VFE616
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15137-1
Original site URL
Representative seq. ID VFE616P
(Link to Original site)
Representative DNA sequence 
>VFE616 (VFE616Q) /CSM/VF/VFE6-A/VFE616Q.Seq.d/
ATACACTCAAAACTCTTAATAAATATATAATATACAATGACTTGGCAAGCATACGTCGAT
AACAACTTACTTGGTGCTGGTTTCGCATCAGCTGCTCTTTTAGGTGCTGCTGACGGTAGT
GTTTGGGCACACTCAGCTGGATTCAATGTCGCTGAAGGTAAGGCAATAACCGCACTTTTT
CAAAAGGATGGAGCTGCATTCGCAACTGGCATCCACGTTGCTGGTAAGAAATATATGGCC
ATCAAAAGTGACACCAGATCTGCTTACGGTAAATTAGGTGCTGGTGGTGTTGTTTGCGTT
AAAACCTTAACTTGTATCATCGTTGCTGTCTATGATGACAAATTACAACCAGGTGCTGCT
GCTAACATTGCTGAAAAATTAGCCGATTATTTAATTGACAACAACTGTTAAATTAGTATT
CTTTTCAAAAATAAATAACGTAAAATAATATATAAAATAATAATAAAAAAATGTTGTAAC
TCTATAXXXXXXXXXXATACACTCAAAACTCTTAATAAATATATAATATACAATGACTTG
GCAAGCATACGTCGATAACAACTTACTTGGTGCTGGTTTCGCATCAGCTGCTCTTTTAGG
TGCTGCTGACGGTAGTGTTTGGGCACACTCAGCTGGATTCAATGTCGCTGAAGGTAAGGC
AATAACCGCACTTTTTCAAAAGGATGGAGCTGCATTCGCAACTGGCATCCACGTTGCTGG
TAAGAAATATATGGCCATCAAAAGTGACACCAGATCTGCTTACGGTAAATTAGGTGCTGG
TGGTGTTGTTTGCGTTAAAACCTTAACTTGTATCATCGTTGCTGTCTATGATGACAAATT
ACAACCAGGTGCTGCTGCTAACATTGCTGAAAAATTAGCCGATTATTTAATTGACAACAA
CTGTTAAATTAGTATTCTTTTCAAAAATAAATAACGTAAAATAATATATAAAATAATAAT
AAAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ihskllini*ytmtwqayvdnnllgagfasaallgaadgsvwahsagfnvaegkaitalf
qkdgaafatgihvagkkymaiksdtrsaygklgaggvvcvktltciivavyddklqpgaa
aniaekladylidnnc*isilfknk*RKIIYKIIIKKCCNSI---

---TLKTLNKYIIYNDLASIRR*qltwcwfriscsfrcc*r*clgtlswiqcr*r*gnnr
tfskgwscirnwhprcw*eiyghqk*hqiclr*ircwwcclr*nlnlyhrccl**qittr
ccc*hc*kisrlfn*qqlln*ysfqk*it*nni*nnnkk
Translated Amino Acid sequence (All Frames) 
Frame A:
ihskllini*ytmtwqayvdnnllgagfasaallgaadgsvwahsagfnvaegkaitalf
qkdgaafatgihvagkkymaiksdtrsaygklgaggvvcvktltciivavyddklqpgaa
aniaekladylidnnc*isilfknk*RKIIYKIIIKKCCNSI---

---ihskllini*ytmtwqayvdnnllgagfasaallgaadgsvwahsagfnvaegkait
alfqkdgaafatgihvagkkymaiksdtrsaygklgaggvvcvktltciivavyddklqp
gaaaniaekladylidnnc*isilfknk*rkiiykiiik

Frame B:
ytqns**iyniq*lgkhtsittylvlvshqllf*vlltvvfghtqldsmslkvrq*phff
krmelhsqlastllvrniwpskvtpdlltvn*vlvvlfalkp*lvssllsmmtnynqvll
ltllkn*pii*ltttvklvffskinnvk*yik***knvvtl---

---ytqns**iyniq*lgkhtsittylvlvshqllf*vlltvvfghtqldsmslkvrq*p
hffkrmelhsqlastllvrniwpskvtpdlltvn*vlvvlfalkp*lvssllsmmtnynq
vllltllkn*pii*ltttvklvffskinnvk*yik***k

Frame C:
tlktlnkyiiyndlasirr*qltwcwfriscsfrcc*r*clgtlswiqcr*r*gnnrtfs
kgwscirnwhprcw*eiyghqk*hqiclr*ircwwcclr*nlnlyhrccl**qittrccc
*hc*kisrlfn*qqlln*ysfqk*it*nni*nnnkkml*ly---

---TLKTLNKYIIYNDLASIRR*qltwcwfriscsfrcc*r*clgtlswiqcr*r*gnnr
tfskgwscirnwhprcw*eiyghqk*hqiclr*ircwwcclr*nlnlyhrccl**qittr
ccc*hc*kisrlfn*qqlln*ysfqk*it*nni*nnnkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHN529 (VHN529Q) /CSM/VH/VHN5-B/VHN529Q.Seq.d/                        844   0.0
VHH513 (VHH513Q) /CSM/VH/VHH5-A/VHH513Q.Seq.d/                        844   0.0
VFK707 (VFK707Q) /CSM/VF/VFK7-A/VFK707Q.Seq.d/                        844   0.0
VFI876 (VFI876Q) /CSM/VF/VFI8-D/VFI876Q.Seq.d/                        844   0.0
VFE616 (VFE616Q) /CSM/VF/VFE6-A/VFE616Q.Seq.d/                        844   0.0
VSH127 (VSH127Q) /CSM/VS/VSH1-B/VSH127Q.Seq.d/                        827   0.0
SSL648 (SSL648Q) /CSM/SS/SSL6-B/SSL648Q.Seq.d/                        817   0.0
SSH172 (SSH172Q) /CSM/SS/SSH1-C/SSH172Q.Seq.d/                        817   0.0
FC-AX21 (FC-AX21Q) /CSM/FC/FC-AX/FC-AX21Q.Seq.d/                      817   0.0
SSJ209 (SSJ209Q) /CSM/SS/SSJ2-A/SSJ209Q.Seq.d/                        815   0.0
own update 2002.12.17
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X61580|X61580.1 D.discoideum mRNA for profilin II.               779  0.0  1
BZ215884|BZ215884.1 CH230-332M13.TV CHORI-230 Segment 2 Rattus norvegicus genomic clone CH230-332M13, DNA sequence.    44  2e-06  2
AC136243|AC136243.1 Rattus norvegicus clone CH230-14L1, *** SEQUENCING IN PROGRESS ***, 68 unordered pieces.    44  3e-05  3
AJ430084|AJ430084.1 Dictyostelium discoideum p3 gene for profilin III.    40  2e-04  3
AC134501|AC134501.2 Rattus norvegicus clone CH230-332M13, WORKING DRAFT SEQUENCE, 3 unordered pieces.    44  4e-04  2
BH946669|BH946669.1 obu86g03.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    54  0.003  1
BZ061901|BZ061901.1 ljq72h01.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    54  0.003  1
AB028622|AB028622.1 Arabidopsis thaliana genomic DNA, chromosome 3, P1 clone:MZN24.    40  0.077  3
AP005815|AP005815.1 Homo sapiens genomic DNA, chromosome 11q clone:CMF9-380I9, WORKING DRAFT SEQUENCE, 3 unordered pieces.    38  0.21  2
BH726784|BH726784.1 BOHUE41TF BO_2_3_KB Brassica oleracea genomic clone BOHUE41, DNA sequence.    38  0.24  2
dna update 2003. 9.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P26200) RecName: Full=Profilin-2; AltName: Full=Profilin II;  &...   213   1e-53
(Q95VF7) RecName: Full=Profilin-1B; AltName: Full=Profilin IB; A...   105   3e-21
(P68696) RecName: Full=Profilin-1A; AltName: Full=Profilin IA; A...   103   7e-21
C48405(C48405)profilin-IB - Acanthamoeba sp. (tentative sequence)     103   1e-20
NRL(1ACF) Profilin i - Acanthamoeba castellanii                       102   3e-20
NRL(2PRF) Profilin ia (nmr, 19 structures) - Acanthamoeba sp.         102   3e-20
(P39825) RecName: Full=Profilin;  &A53952(A53952;T38785) &CU3296...    97   8e-19
S13199(S13199)profilin - slime mold (Physarum polycephalum)            97   8e-19
(Q8T8M2) RecName: Full=Profilin-3; AltName: Full=Profilin III;  ...    96   2e-18
CR382128_274(CR382128|pid:none) Yarrowia lipolytica strain CLIB1...    89   2e-16
protein update 2009. 6.19
PSORT 

 psg: 0.73  gvh: 0.30  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.46  mip: 0.00
 nuc: 0.12  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 16.0 %: nuclear
	  4.0 %: peroxisomal

>> prediction for VFE616 is mit
5' end seq. ID VFE616F
5' end seq. 
>VFE616F.Seq
ATACACTCAAAACTCTTAATAAATATATAATATACAATGACTTGGCAAGCATACGTCGAT
AACAACTTACTTGGTGCTGGTTTCGCATCAGCTGCTCTTTTAGGTGCTGCTGACGGTAGT
GTTTGGGCACACTCAGCTGGATTCAATGTCGCTGAAGGTAAGGCAATAACCGCACTTTTT
CAAAAGGATGGAGCTGCATTCGCAACTGGCATCCACGTTGCTGGTAAGAAATATATGGCC
ATCAAAAGTGACACCAGATCTGCTTACGGTAAATTAGGTGCTGGTGGTGTTGTTTGCGTT
AAAACCTTAACTTGTATCATCGTTGCTGTCTATGATGACAAATTACAACCAGGTGCTGCT
GCTAACATTGCTGAAAAATTAGCCGATTATTTAATTGACAACAACTGTTAAATTAGTATT
CTTTTCAAAAATAAATAACGTAAAATAATATATAAAATAATAATAAAAAAATGTTGTAAC
TCTATA----------
Length of 5' end seq. 486
3' end seq. ID VFE616Z
3' end seq. 
>VFE616Z.Seq
----------ATACACTCAAAACTCTTAATAAATATATAATATACAATGACTTGGCAAGC
ATACGTCGATAACAACTTACTTGGTGCTGGTTTCGCATCAGCTGCTCTTTTAGGTGCTGC
TGACGGTAGTGTTTGGGCACACTCAGCTGGATTCAATGTCGCTGAAGGTAAGGCAATAAC
CGCACTTTTTCAAAAGGATGGAGCTGCATTCGCAACTGGCATCCACGTTGCTGGTAAGAA
ATATATGGCCATCAAAAGTGACACCAGATCTGCTTACGGTAAATTAGGTGCTGGTGGTGT
TGTTTGCGTTAAAACCTTAACTTGTATCATCGTTGCTGTCTATGATGACAAATTACAACC
AGGTGCTGCTGCTAACATTGCTGAAAAATTAGCCGATTATTTAATTGACAACAACTGTTA
AATTAGTATTCTTTTCAAAAATAAATAACGTAAAATAATATATAAAATAATAATAAAAAA
Length of 3' end seq. 470
Connected seq. ID VFE616P
Connected seq. 
>VFE616P.Seq
ATACACTCAAAACTCTTAATAAATATATAATATACAATGACTTGGCAAGCATACGTCGAT
AACAACTTACTTGGTGCTGGTTTCGCATCAGCTGCTCTTTTAGGTGCTGCTGACGGTAGT
GTTTGGGCACACTCAGCTGGATTCAATGTCGCTGAAGGTAAGGCAATAACCGCACTTTTT
CAAAAGGATGGAGCTGCATTCGCAACTGGCATCCACGTTGCTGGTAAGAAATATATGGCC
ATCAAAAGTGACACCAGATCTGCTTACGGTAAATTAGGTGCTGGTGGTGTTGTTTGCGTT
AAAACCTTAACTTGTATCATCGTTGCTGTCTATGATGACAAATTACAACCAGGTGCTGCT
GCTAACATTGCTGAAAAATTAGCCGATTATTTAATTGACAACAACTGTTAAATTAGTATT
CTTTTCAAAAATAAATAACGTAAAATAATATATAAAATAATAATAAAAAAATGTTGTAAC
TCTATA----------ATACACTCAAAACTCTTAATAAATATATAATATACAATGACTTG
GCAAGCATACGTCGATAACAACTTACTTGGTGCTGGTTTCGCATCAGCTGCTCTTTTAGG
TGCTGCTGACGGTAGTGTTTGGGCACACTCAGCTGGATTCAATGTCGCTGAAGGTAAGGC
AATAACCGCACTTTTTCAAAAGGATGGAGCTGCATTCGCAACTGGCATCCACGTTGCTGG
TAAGAAATATATGGCCATCAAAAGTGACACCAGATCTGCTTACGGTAAATTAGGTGCTGG
TGGTGTTGTTTGCGTTAAAACCTTAACTTGTATCATCGTTGCTGTCTATGATGACAAATT
ACAACCAGGTGCTGCTGCTAACATTGCTGAAAAATTAGCCGATTATTTAATTGACAACAA
CTGTTAAATTAGTATTCTTTTCAAAAATAAATAACGTAAAATAATATATAAAATAATAAT
AAAAAA
Length of connected seq. 956
Full length Seq ID -
Full length Seq. -
Length of full length seq. -