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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM418
Library AF
(Link to library)
Clone ID AFM418
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM418P
(Link to Original site)
Representative DNA sequence 
>AFM418 (AFM418Q) /CSM/AF/AFM4-A/AFM418Q.Seq.d/
ACACACATACTTTCATTTAATTATATTACATACTTTTATTTCAATAATTATATATTTAAG
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANGGNNCNNNTTTTTAAAAAAAAAAXXXXX
XXXXXAATTNNTNCAAANTTNAAATTAANNTTTTTNAAANNAANGGAAATTNTTTNNANG
GTTTNAAAAAAAAAAAAGGGGGGNANTTTTTTTTTTTTNTTTTTTTTTTTAANAAAACCA
NAGGGAGGGGGTTTGTTGTTTATAT
sequence update 2009. 4. 3
Translated Amino Acid sequence 
THILSFNYITYFYFNNYIFKEKKKKKKKKKXXXXFKKK---

---NXXKXXIXXFXXXGNXXXGXKKKKGGXFFFFXFFFXKTXGRGFVVY
Translated Amino Acid sequence (All Frames) 
Frame A:
THILSFNYITYFYFNNYIFKEKKKKKKKKKXXXXFKKK---

---NXXKXXIXXFXXXGNXXXGXKKKKGGXFFFFXFFFXKTXGRGFVVY

Frame B:
htyfhliilhtfisiiiylrkkkkkkkkkkxxxflkkk---

---ixxxxklxfxkxxeixxxvxkkkkggxfffxffflxkpxgggllfi

Frame C:
thtfi*lyyillfq*lyi*gkkkkkkkkkxgxxf*kk---

---xxqxxn*xfxxxxkxfxxfxkkkrgxxfffxfff*xnxregvccly
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM418 (AFM418Q) /CSM/AF/AFM4-A/AFM418Q.Seq.d/                        121   6e-27
SFH239 (SFH239Q) /CSM/SF/SFH2-B/SFH239Q.Seq.d/                         36   0.25
SFD266 (SFD266Q) /CSM/SF/SFD2-C/SFD266Q.Seq.d/                         36   0.25
CFJ829 (CFJ829Q) /CSM/CF/CFJ8-B/CFJ829Q.Seq.d/                         36   0.25
AFI638 (AFI638Q) /CSM/AF/AFI6-B/AFI638Q.Seq.d/                         36   0.25
VSI341 (VSI341Q) /CSM/VS/VSI3-B/VSI341Q.Seq.d/                         34   1.00
VHK631 (VHK631Q) /CSM/VH/VHK6-B/VHK631Q.Seq.d/                         34   1.00
VHJ752 (VHJ752Q) /CSM/VH/VHJ7-C/VHJ752Q.Seq.d/                         34   1.00
VHG206 (VHG206Q) /CSM/VH/VHG2-A/VHG206Q.Seq.d/                         34   1.00
VHF383 (VHF383Q) /CSM/VH/VHF3-D/VHF383Q.Seq.d/                         34   1.00
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ329460) Dictyostelium discoideum cDNA clone:dda25d06, 5' ...   121   1e-23  1
(BJ346786) Dictyostelium discoideum cDNA clone:dda25d06, 3' ...    58   2e-04  1
(BX908769) Zebrafish DNA sequence from clone DKEY-78I1 in li...    50   0.041  1
(CU929554) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    50   0.041  1
(CU929520) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    50   0.041  1
(CT583682) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    50   0.041  1
(FB623875) Sequence 39598 from Patent WO2004081186.                48   0.16   1
(FB623874) Sequence 39597 from Patent WO2004081186.                48   0.16   1
(FB623861) Sequence 39584 from Patent WO2004081186.                48   0.16   1
(FB623860) Sequence 39583 from Patent WO2004081186.                48   0.16   1
dna update 2009. 4.11
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 105,060,567
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM418 is

5' end seq. ID AFM418F
5' end seq. 
>AFM418F.Seq
ACACACATACTTTCATTTAATTATATTACATACTTTTATTTCAATAATTATATATTTAAG
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANGGNNCNNNTTTTTAAAAAAAAAAXXXXX
XXXXX
Length of 5' end seq. 125
3' end seq. ID AFM418Z
3' end seq. 
>AFM418Z.Seq
XXXXXXXXXXAATTNNTNCAAANTTNAAATTAANNTTTTTNAAANNAANGGAAATTNTTT
NNANGGTTTNAAAAAAAAAAAAGGGGGGNANTTTTTTTTTTTTNTTTTTTTTTTTAANAA
AACCANAGGGAGGGGGTTTGTTGTTTATAT
Length of 3' end seq. 150
Connected seq. ID AFM418P
Connected seq. 
>AFM418P.Seq
ACACACATACTTTCATTTAATTATATTACATACTTTTATTTCAATAATTATATATTTAAG
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANGGNNCNNNTTTTTAAAAAAAAAA-----
-----AATTNNTNCAAANTTNAAATTAANNTTTTTNAAANNAANGGAAATTNTTTNNANG
GTTTNAAAAAAAAAAAAGGGGGGNANTTTTTTTTTTTTNTTTTTTTTTTTAANAAAACCA
NAGGGAGGGGGTTTGTTGTTTATAT
Length of connected seq. 255
Full length Seq ID -
Full length Seq. -
Length of full length seq. -