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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFE580
Library VF
(Link to library)
Clone ID VFE580
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14931-1
Original site URL
Representative seq. ID VFE580P
(Link to Original site)
Representative DNA sequence 
>VFE580 (VFE580Q) /CSM/VF/VFE5-D/VFE580Q.Seq.d/
ACTGTTGGCCTACTGGNAATTTTTAACAAATCATTAATCAAAATGTCTTCAAATAAAATT
GGTAATAAATTAAGTCTTGACAAGGTAGATGTAAAAGGTAAAAGAGTTTTAATTATGAGT
TGATTATAATGTACCATTAGATAAAAATAATAATATTACAAGTACTCAAAGAATTGATGC
ATNAATTCCAACATTAGAATATTGNTTAAAGAATGGTGCTAAATCAATTGTATTGATGTC
ACATTTAGGNCGTXXXXXXXXXXTTCAAGATCAAGGTATCCCAGAAGGTTGNATGGGTTT
AGATTGTGGTCCAGAGACTATCAAAGAAANCAGAGATACCATCTCAAGAGCAAAAACCAT
CGTTTGGAATGGTCCTATGGGTGTATTTGAAAAGAGCAATTTCGAAGCCGGTACAAAAGC
TGCCATGGATGATGTTGTCAATGCCACAACCAATGGTGCTATCACTATCATTGGTGGTGG
TGATACCGCCACTTGTGCTGCCAAATACAACACTGAAGATAAAGTTAGTCATGTCTCAAC
TGGTGGTGGTGCTTCATTAGAATATTAGAAGGTAAGAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
llaywxfltnh*skclqiklvin*vltr*m*kvkef*l*VDYNVPLDKNNNITSTQRIDA
XIPTLEYXLKNGAKSIVLMSHLGR---

---QDQGIPEGXMGLDCGPETIKEXRDTISRAKTIVWNGPMGVFEKSNFEAGTKAAMDDV
VNATTNGAITIIGGGDTATCAAKYNTEDKVSHVSTGGGASLEY*kvr
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxifnkslikmssnkignklsldkvdvkgkrvlims*l*ctir*k**yykyskn*c
xnsnirilxkewc*incidvtfrx---

---fkikvsqkvxwv*ivvqrlskkxeipsqeqkpsfgmvlwvylkraiskpvqklpwmm
lsmpqpmvlslslvvvipplvlpnttlkiklvmsqlvvvlh*nirr*e

Frame B:
llaywxfltnh*skclqiklvin*vltr*m*kvkef*l*VDYNVPLDKNNNITSTQRIDA
XIPTLEYXLKNGAKSIVLMSHLGR---

---srsryprrlxgfrlwsrdyqrxqryhlksknhrlewsygci*keqfrsrykschg*c
cqchnqwcyhyhwww*yrhlccqiqh*r*s*sclnwwwcfirilegkn

Frame C:
cwptgnf*qiinqnvfk*nw**iks*qgrckr*ksfnyeliimyh*ikiiilqvlkelmh
xfqh*nix*rmvlnqly*chi*x---

---QDQGIPEGXMGLDCGPETIKEXRDTISRAKTIVWNGPMGVFEKSNFEAGTKAAMDDV
VNATTNGAITIIGGGDTATCAAKYNTEDKVSHVSTGGGASLEY*kvr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFE580 (VFE580Q) /CSM/VF/VFE5-D/VFE580Q.Seq.d/                       1053   0.0
SFD565 (SFD565Q) /CSM/SF/SFD5-C/SFD565Q.Seq.d/                        605   e-172
VSI344 (VSI344Q) /CSM/VS/VSI3-B/VSI344Q.Seq.d/                        591   e-168
VSD369 (VSD369Q) /CSM/VS/VSD3-C/VSD369Q.Seq.d/                        591   e-168
VHD789 (VHD789Q) /CSM/VH/VHD7-D/VHD789Q.Seq.d/                        591   e-168
VFO749 (VFO749Q) /CSM/VF/VFO7-C/VFO749Q.Seq.d/                        591   e-168
VFO594 (VFO594Q) /CSM/VF/VFO5-D/VFO594Q.Seq.d/                        591   e-168
VFN471 (VFN471Q) /CSM/VF/VFN4-C/VFN471Q.Seq.d/                        591   e-168
VFL513 (VFL513Q) /CSM/VF/VFL5-A/VFL513Q.Seq.d/                        591   e-168
VFL140 (VFL140Q) /CSM/VF/VFL1-B/VFL140Q.Seq.d/                        591   e-168
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF316577|AF316577.2 Dictyostelium discoideum phosphoglycerate kinase (pgkA) mRNA, complete cds.   581  0.0  4
AF018637|AF018637.1 Dictyostelium discoideum phosphoglycerate kinase (pgkA) mRNA, partial cds.   581  e-162  2
BU776741| (L36833) phosphoglycerate kinase [Schistosoma mansoni], mRNA sequence.    62  2e-13  2
BU766685| (L36833) phosphoglycerate kinase [Schistosoma mansoni], mRNA sequence.    62  2e-13  2
BU771972|PGK_SCHMA PHOSPHOGLYCERATE KINASE, mRNA sequence.    62  2e-13  2
BU724812|PGK_SCHMA PHOSPHOGLYCERATE KINASE, mRNA sequence.    62  3e-13  2
BU722559|PGK_SCHMA PHOSPHOGLYCERATE KINASE, mRNA sequence.    62  3e-13  2
BU725857|PGK_SCHMA PHOSPHOGLYCERATE KINASE, mRNA sequence.    62  3e-13  2
BU715686|PGK_SCHMA PHOSPHOGLYCERATE KINASE, mRNA sequence.    62  3e-13  2
BU773106| (L36833) phosphoglycerate kinase [Schistosoma mansoni], mRNA sequence.    62  3e-13  2
dna update 2003. 9.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9GPM4) RecName: Full=Phosphoglycerate kinase;          EC=2.7....   152   6e-36
AF018637_1(AF018637|pid:none) Dictyostelium discoideum phosphogl...   152   6e-36
AY309061_1(AY309061|pid:none) Schistosoma japonicum phosphoglyce...   118   1e-25
FN315990_1(FN315990|pid:none) Schistosoma japonicum isolate Anhu...   118   1e-25
EZ000104_1(EZ000104|pid:none) TSA: Schistosoma japonicum SJCHGC0...   118   1e-25
AY223444_1(AY223444|pid:none) Schistosoma japonicum clone ZZZ452...   118   1e-25
FN322120_1(FN322120|pid:none) Schistosoma japonicum isolate Anhu...   118   1e-25
FN315993_1(FN315993|pid:none) Schistosoma japonicum isolate Anhu...   116   4e-25
BC110004_1(BC110004|pid:none) Bos taurus phosphoglycerate kinase...   112   6e-24
AY826867_1(AY826867|pid:none) Guillardia theta clone GTPGK1 phos...   111   1e-23
protein update 2009. 6.19
PSORT 

 psg: 0.75  gvh: 0.48  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VFE580 is cyt
5' end seq. ID VFE580F
5' end seq. 
>VFE580F.Seq
ACTGTTGGCCTACTGGNAATTTTTAACAAATCATTAATCAAAATGTCTTCAAATAAAATT
GGTAATAAATTAAGTCTTGACAAGGTAGATGTAAAAGGTAAAAGAGTTTTAATTATGAGT
TGATTATAATGTACCATTAGATAAAAATAATAATATTACAAGTACTCAAAGAATTGATGC
ATNAATTCCAACATTAGAATATTGNTTAAAGAATGGTGCTAAATCAATTGTATTGATGTC
ACATTTAGGNCGT----------
Length of 5' end seq. 253
3' end seq. ID VFE580Z
3' end seq. 
>VFE580Z.Seq
----------TTCAAGATCAAGGTATCCCAGAAGGTTGNATGGGTTTAGATTGTGGTCCA
GAGACTATCAAAGAAANCAGAGATACCATCTCAAGAGCAAAAACCATCGTTTGGAATGGT
CCTATGGGTGTATTTGAAAAGAGCAATTTCGAAGCCGGTACAAAAGCTGCCATGGATGAT
GTTGTCAATGCCACAACCAATGGTGCTATCACTATCATTGGTGGTGGTGATACCGCCACT
TGTGCTGCCAAATACAACACTGAAGATAAAGTTAGTCATGTCTCAACTGGTGGTGGTGCT
TCATTAGAATATTAGAAGGTAAGAAT
Length of 3' end seq. 316
Connected seq. ID VFE580P
Connected seq. 
>VFE580P.Seq
ACTGTTGGCCTACTGGNAATTTTTAACAAATCATTAATCAAAATGTCTTCAAATAAAATT
GGTAATAAATTAAGTCTTGACAAGGTAGATGTAAAAGGTAAAAGAGTTTTAATTATGAGT
TGATTATAATGTACCATTAGATAAAAATAATAATATTACAAGTACTCAAAGAATTGATGC
ATNAATTCCAACATTAGAATATTGNTTAAAGAATGGTGCTAAATCAATTGTATTGATGTC
ACATTTAGGNCGT----------TTCAAGATCAAGGTATCCCAGAAGGTTGNATGGGTTT
AGATTGTGGTCCAGAGACTATCAAAGAAANCAGAGATACCATCTCAAGAGCAAAAACCAT
CGTTTGGAATGGTCCTATGGGTGTATTTGAAAAGAGCAATTTCGAAGCCGGTACAAAAGC
TGCCATGGATGATGTTGTCAATGCCACAACCAATGGTGCTATCACTATCATTGGTGGTGG
TGATACCGCCACTTGTGCTGCCAAATACAACACTGAAGATAAAGTTAGTCATGTCTCAAC
TGGTGGTGGTGCTTCATTAGAATATTAGAAGGTAAGAAT
Length of connected seq. 569
Full length Seq ID -
Full length Seq. -
Length of full length seq. -