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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM409
Library AF
(Link to library)
Clone ID AFM409
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM409P
(Link to Original site)
Representative DNA sequence 
>AFM409 (AFM409Q) /CSM/AF/AFM4-A/AFM409Q.Seq.d/
ATTTTGTATTATATATTTAAATAATAATGCAATCACTTGCACGTTTATCTCGTACCTCTT
TGGTTCAAAAGGGTCTTGTTCCACAAACCATTAAAAACTATTCATCAGTTTCACAAGCTG
AAATTGATAATGAACCAAGATTTTTAGAATGTTTCAAAACTTTCTTTGATAAAGCAGCTG
GTTTAACCAATTTAAAACCAGGTGTTTTAAATAATATGAAAGAATGTAATGTTGCTCTCC
GTGTTGAATTCCCAATCAAGAATGAACATGGTGATGTTGATATTATTGCAGGTTATCGTG
CACAACATTCACACCATCGTTTACCATGCAAAGGTGGTATTCGTTTCAGTGAAGAAGTAG
ATCTCCAAGAAGTTATGGCCTTAGCTTCATTGATGACTTATAAATGTGCNTGTCGTCGAT
GTTCCATTTGGTGGTGCTAAAGGTGGTGTCCGTATCGACCCAAAGAAATACACTGTCGCC
CAACGTGAAAAGATCACTCGTGCCTACACTCTCCTCTTATGTCAAAAGAATTTCATCGGA
CCAXXXXXXXXXXCTTTGAACAAGCTGGTGCTAAAGTTATTGCCGTCGCCGAACACAATG
GTGCCGTCTACAACGCCGAAGGTCTCAACATTGATGCCCTCAACAAATATAAACTCCAAC
ACGGTACTTTCATTGATTTCCCAGGTGCCACCAACATTGTCGACTCTGTCAAAGCCTTAG
AGATCCCATGTGATATCTTAATCCCAGCTGCCCTCGAAAAACAAATCCACATTGGTAACG
TCGCCGATATTCAAGCTAAATTAATTGGTGAAGCTGCCAACGGTCCAATGACCCCACGTG
CTGACCAAATCCTCCTCAATAGAGGTCATGTCATCATTCCAGATCTCCTCTTAAATGCTG
GTGGTGTCACTGTATCATACTTTGAATGGTTAAAGAATCTTTCTCACGTTCGTTTCGGTC
GTTTGAACAAGAAATGGGAAGAATCCTCAAAGAAATTATTATTAGAATTTGTTGAATCAA
CCGTCAACAAGAAATTATCAGAAGCTGAAAGAAGCTTAATCATCCATGGTGCTGATGAAA
TCGATATCGTTCGTTCAGGTCTCGAAGATACCATGCAAAATGCTTGTGCTGAAACCAGAA
AGACTGCAAATGAAAAGAACACTGATTACCGTTCTGCCGCTTTATACAATGCTATCATGA
AGATTAAAGCTGTCTATGAATCATCGGTAATGTCTTCTCTTAAATATTTTAAAAAAACCC
TTTTAAG
sequence update 2009. 4. 3
Translated Amino Acid sequence 
ilyyifk**cnhlhvylvplwfkrvlfhkplktihqfhklklimnqdf*nvsklslikql
v*pi*nqvf*ii*knvmllsvlnsqsrmnmvmlillqvivhnihtivyhakvvfvsvkk*
iskklwp*lh**LINVXVVDVPFGGAKGGVRIDPKKYTVAQREKITRAYTLLLCQKNFIG
P---

---FEQAGAKVIAVAEHNGAVYNAEGLNIDALNKYKLQHGTFIDFPGATNIVDSVKALEI
PCDILIPAALEKQIHIGNVADIQAKLIGEAANGPMTPRADQILLNRGHVIIPDLLLNAGG
VTVSYFEWLKNLSHVRFGRLNKKWEESSKKLLLEFVESTVNKKLSEAERSLIIHGADEID
IVRSGLEDTMQNACAETRKTANEKNTDYRSAALYNAIMKIKAVYESSVMSSLKYFKKTLL
Translated Amino Acid sequence (All Frames) 
Frame A:
ilyyifk**cnhlhvylvplwfkrvlfhkplktihqfhklklimnqdf*nvsklslikql
v*pi*nqvf*ii*knvmllsvlnsqsrmnmvmlillqvivhnihtivyhakvvfvsvkk*
iskklwp*lh**LINVXVVDVPFGGAKGGVRIDPKKYTVAQREKITRAYTLLLCQKNFIG
P---

---l*tswc*sycrrrtqwcrlqrrrsqh*cpqqi*tptryfh*fprchqhcrlcqslrd
pm*ylnpscprktnphw*rrryss*inw*scqrsndptc*pnppq*rschhsrsplkcww
chciil*mvkesfsrsfrsfeqemgrilkeiiiric*inrqqeiirs*kklnhpwc**nr
yrsfrsrryhakclc*nqkdck*keh*lpfcrfiqcyhed*scl*iignvfs*if*knpf
k

Frame B:
fciiylnnnaitctfisylfgskgscstnh*klfisfts*n***tkifrmfqnfl**ssw
fnqfktrcfk*yerm*ccspc*ipnqe*tw*c*yycrlscttftpsftmqrwysfq*rsr
sprsyglsfiddl*mcxssmfhlvvlkvvsvstqrntlspnvkrslvptlssyvkrissd
---

---FEQAGAKVIAVAEHNGAVYNAEGLNIDALNKYKLQHGTFIDFPGATNIVDSVKALEI
PCDILIPAALEKQIHIGNVADIQAKLIGEAANGPMTPRADQILLNRGHVIIPDLLLNAGG
VTVSYFEWLKNLSHVRFGRLNKKWEESSKKLLLEFVESTVNKKLSEAERSLIIHGADEID
IVRSGLEDTMQNACAETRKTANEKNTDYRSAALYNAIMKIKAVYESSVMSSLKYFKKTLL

Frame C:
fvlyi*iimqslarlsrtslvqkglvpqtiknyssvsqaeidneprflecfktffdkaag
ltnlkpgvlnnmkecnvalrvefpiknehgdvdiiagyraqhshhrlpckggirfseevd
lqevmalaslmtykcacrrcsiwwc*rwcpyrpkeihcrpt*kdhsclhsplmskefhrt
---

---lnklvlkllpspntmvpsttpkvstlmpstninsntvlslisqvpptlstlskp*rs
hvis*sqlpsknkstlvtspifkln*lvklptvq*phvltksssievmssfqiss*mlvv
slyhtlng*rifltfvsvv*trngknpqrnyy*nllnqpstrnyqklkea*ssmvlmksi
sfvqvskipckmlvlkperlqmkrtlitvlplytmls*rlklsmnhr*clllnilkkpf*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM409 (AFM409Q) /CSM/AF/AFM4-A/AFM409Q.Seq.d/                       2413   0.0
SFI734 (SFI734Q) /CSM/SF/SFI7-B/SFI734Q.Seq.d/                       1368   0.0
SLG743 (SLG743Q) /CSM/SL/SLG7-B/SLG743Q.Seq.d/                       1360   0.0
SLE716 (SLE716Q) /CSM/SL/SLE7-A/SLE716Q.Seq.d/                       1360   0.0
SFG859 (SFG859Q) /CSM/SF/SFG8-C/SFG859Q.Seq.d/                       1360   0.0
CFF488 (CFF488Q) /CSM/CF/CFF4-D/CFF488Q.Seq.d/                       1360   0.0
CFE718 (CFE718Q) /CSM/CF/CFE7-A/CFE718Q.Seq.d/                       1360   0.0
AFN728 (AFN728Q) /CSM/AF/AFN7-B/AFN728Q.Seq.d/                       1360   0.0
AFC621 (AFC621Q) /CSM/AF/AFC6-A/AFC621Q.Seq.d/                       1358   0.0
VFD217 (VFD217Q) /CSM/VF/VFD2-A/VFD217Q.Seq.d/                       1354   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ346774) Dictyostelium discoideum cDNA clone:dda25b04, 3' ...  1382   0.0    1
(BJ402787) Dictyostelium discoideum cDNA clone:dds18d09, 3' ...  1368   0.0    1
(AU039915) Dictyostelium discoideum slug cDNA, clone SLG743.     1324   0.0    2
(AU039538) Dictyostelium discoideum slug cDNA, clone SLE716.     1324   0.0    2
(BJ372285) Dictyostelium discoideum cDNA clone:ddc11p22, 3' ...  1324   0.0    2
(BJ371862) Dictyostelium discoideum cDNA clone:ddc10d05, 3' ...  1324   0.0    2
(BJ345643) Dictyostelium discoideum cDNA clone:dda28h07, 3' ...  1324   0.0    2
(BJ400736) Dictyostelium discoideum cDNA clone:dds14f16, 3' ...  1322   0.0    2
(BJ430360) Dictyostelium discoideum cDNA clone:ddv7a06, 3' e...  1318   0.0    2
(BJ346827) Dictyostelium discoideum cDNA clone:dda25o02, 3' ...  1318   0.0    2
dna update 2009. 4.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54KB7) RecName: Full=Glutamate dehydrogenase, mitochondrial;  ...   396   e-109
CP000159_2202(CP000159|pid:none) Salinibacter ruber DSM 13855, c...   184   7e-45
CP000760_126(CP000760|pid:none) Ochrobactrum anthropi ATCC 49188...   183   1e-44
CP000362_1668(CP000362|pid:none) Roseobacter denitrificans OCh 1...   182   2e-44
(P82264) RecName: Full=Glutamate dehydrogenase, mitochondrial;  ...   179   2e-43
EF107099_5(EF107099|pid:none) Uncultured marine bacterium EB0_49...   178   3e-43
BT059207_1(BT059207|pid:none) Salmo salar clone ssal-rgf-532-192...   177   7e-43
BT072604_1(BT072604|pid:none) Salmo salar clone ssal-rgf-532-071...   176   1e-42
BT044837_1(BT044837|pid:none) Salmo salar clone ssal-rgf-505-100...   176   1e-42
AJ556996_1(AJ556996|pid:none) Oncorhynchus mykiss partial mRNA f...   176   2e-42
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM409 is

5' end seq. ID AFM409F
5' end seq. 
>AFM409F.Seq
ATTTTGTATTATATATTTAAATAATAATGCAATCACTTGCACGTTTATCTCGTACCTCTT
TGGTTCAAAAGGGTCTTGTTCCACAAACCATTAAAAACTATTCATCAGTTTCACAAGCTG
AAATTGATAATGAACCAAGATTTTTAGAATGTTTCAAAACTTTCTTTGATAAAGCAGCTG
GTTTAACCAATTTAAAACCAGGTGTTTTAAATAATATGAAAGAATGTAATGTTGCTCTCC
GTGTTGAATTCCCAATCAAGAATGAACATGGTGATGTTGATATTATTGCAGGTTATCGTG
CACAACATTCACACCATCGTTTACCATGCAAAGGTGGTATTCGTTTCAGTGAAGAAGTAG
ATCTCCAAGAAGTTATGGCCTTAGCTTCATTGATGACTTATAAATGTGCNTGTCGTCGAT
GTTCCATTTGGTGGTGCTAAAGGTGGTGTCCGTATCGACCCAAAGAAATACACTGTCGCC
CAACGTGAAAAGATCACTCGTGCCTACACTCTCCTCTTATGTCAAAAGAATTTCATCGGA
CCAXXXXXXXXXX
Length of 5' end seq. 553
3' end seq. ID AFM409Z
3' end seq. 
>AFM409Z.Seq
XXXXXXXXXXCTTTGAACAAGCTGGTGCTAAAGTTATTGCCGTCGCCGAACACAATGGTG
CCGTCTACAACGCCGAAGGTCTCAACATTGATGCCCTCAACAAATATAAACTCCAACACG
GTACTTTCATTGATTTCCCAGGTGCCACCAACATTGTCGACTCTGTCAAAGCCTTAGAGA
TCCCATGTGATATCTTAATCCCAGCTGCCCTCGAAAAACAAATCCACATTGGTAACGTCG
CCGATATTCAAGCTAAATTAATTGGTGAAGCTGCCAACGGTCCAATGACCCCACGTGCTG
ACCAAATCCTCCTCAATAGAGGTCATGTCATCATTCCAGATCTCCTCTTAAATGCTGGTG
GTGTCACTGTATCATACTTTGAATGGTTAAAGAATCTTTCTCACGTTCGTTTCGGTCGTT
TGAACAAGAAATGGGAAGAATCCTCAAAGAAATTATTATTAGAATTTGTTGAATCAACCG
TCAACAAGAAATTATCAGAAGCTGAAAGAAGCTTAATCATCCATGGTGCTGATGAAATCG
ATATCGTTCGTTCAGGTCTCGAAGATACCATGCAAAATGCTTGTGCTGAAACCAGAAAGA
CTGCAAATGAAAAGAACACTGATTACCGTTCTGCCGCTTTATACAATGCTATCATGAAGA
TTAAAGCTGTCTATGAATCATCGGTAATGTCTTCTCTTAAATATTTTAAAAAAACCCTTT
TAAG
Length of 3' end seq. 724
Connected seq. ID AFM409P
Connected seq. 
>AFM409P.Seq
ATTTTGTATTATATATTTAAATAATAATGCAATCACTTGCACGTTTATCTCGTACCTCTT
TGGTTCAAAAGGGTCTTGTTCCACAAACCATTAAAAACTATTCATCAGTTTCACAAGCTG
AAATTGATAATGAACCAAGATTTTTAGAATGTTTCAAAACTTTCTTTGATAAAGCAGCTG
GTTTAACCAATTTAAAACCAGGTGTTTTAAATAATATGAAAGAATGTAATGTTGCTCTCC
GTGTTGAATTCCCAATCAAGAATGAACATGGTGATGTTGATATTATTGCAGGTTATCGTG
CACAACATTCACACCATCGTTTACCATGCAAAGGTGGTATTCGTTTCAGTGAAGAAGTAG
ATCTCCAAGAAGTTATGGCCTTAGCTTCATTGATGACTTATAAATGTGCNTGTCGTCGAT
GTTCCATTTGGTGGTGCTAAAGGTGGTGTCCGTATCGACCCAAAGAAATACACTGTCGCC
CAACGTGAAAAGATCACTCGTGCCTACACTCTCCTCTTATGTCAAAAGAATTTCATCGGA
CCA----------CTTTGAACAAGCTGGTGCTAAAGTTATTGCCGTCGCCGAACACAATG
GTGCCGTCTACAACGCCGAAGGTCTCAACATTGATGCCCTCAACAAATATAAACTCCAAC
ACGGTACTTTCATTGATTTCCCAGGTGCCACCAACATTGTCGACTCTGTCAAAGCCTTAG
AGATCCCATGTGATATCTTAATCCCAGCTGCCCTCGAAAAACAAATCCACATTGGTAACG
TCGCCGATATTCAAGCTAAATTAATTGGTGAAGCTGCCAACGGTCCAATGACCCCACGTG
CTGACCAAATCCTCCTCAATAGAGGTCATGTCATCATTCCAGATCTCCTCTTAAATGCTG
GTGGTGTCACTGTATCATACTTTGAATGGTTAAAGAATCTTTCTCACGTTCGTTTCGGTC
GTTTGAACAAGAAATGGGAAGAATCCTCAAAGAAATTATTATTAGAATTTGTTGAATCAA
CCGTCAACAAGAAATTATCAGAAGCTGAAAGAAGCTTAATCATCCATGGTGCTGATGAAA
TCGATATCGTTCGTTCAGGTCTCGAAGATACCATGCAAAATGCTTGTGCTGAAACCAGAA
AGACTGCAAATGAAAAGAACACTGATTACCGTTCTGCCGCTTTATACAATGCTATCATGA
AGATTAAAGCTGTCTATGAATCATCGGTAATGTCTTCTCTTAAATATTTTAAAAAAACCC
TTTTAAG
Length of connected seq. 1257
Full length Seq ID -
Full length Seq. -
Length of full length seq. -