>VFE480 (VFE480Q) /CSM/VF/VFE4-D/VFE480Q.Seq.d/
XXXXXXXXXXAAGAATTAAAAGATTTAATTTTGCAAACAAAAAATGCTTATTAATTAAAT
ATAGTAAAGATACTAGATATAACGATAACATTGATAAATCATTTTTAGTTACCCATGACA
AACAAAATTATCAAGCTTTCCCATGTTCAATTTTAGAAGATGTTAAAGAACAAGCACAAA
ACTATGATGTAATTGGTATTGATGAAGGCCAATTTTTCCCAGACGTTGTACAATTTAGTG
AAGACTTGGCAAATCAAGGTAAANCCGTTATAATTGCCGCACTAGATGGTACCTTCCAAA
GAAAACCATTCCAATCAGTCATTGATTTAGTATCAAAGGCTGAATATATTACAAAGTTAA
CTGCCGTTTGTATGGTTTGCTACAATGAAGCCGCCTTTTCAAAACGTATCGTCGAAAGTG
ATGATATCGAATTAATTGGTGGTATAGATAAATATATTTCCGTTTGCCGTGGTTGCTACA
ATAGTGATCAAAATGAA

---RIKRFNFANKKCLLIKYSKDTRYNDNIDKSFLVTHDKQNYQAFPCSILEDVKEQAQN
YDVIGIDEGQFFPDVVQFSEDLANQGKXVIIAALDGTFQRKPFQSVIDLVSKAEYITKLT
AVCMVCYNEAAFSKRIVESDDIELIGGIDKYISVCRGCYNSDQNE

Frame A:
---RIKRFNFANKKCLLIKYSKDTRYNDNIDKSFLVTHDKQNYQAFPCSILEDVKEQAQN
YDVIGIDEGQFFPDVVQFSEDLANQGKXVIIAALDGTFQRKPFQSVIDLVSKAEYITKLT
AVCMVCYNEAAFSKRIVESDDIELIGGIDKYISVCRGCYNSDQNE


Frame B:
---elkdlilqtknay*lnivkildititlinhf*lpmtnkiiklshvqf*kmlknkhkt
mm*lvlmkanfsqtlynlvktwqikvxpl*lph*mvpskenhsnqsli*yqrlnilqs*l
pfvwfatmkppfqnvsskvmisn*lvv*inifpfavvativikm


Frame C:
---n*ki*fckqkmlin*i**ry*i*r*h**iifsyp*qtklssfpmfnfrrc*rtstkl
*cnwy**rpifprrcti**rlgksr*xryncrtrwylpkktipish*fsikg*iyykvnc
rlygllq*srlfktyrrk**yrinwwyr*iyfrlpwllq**sk*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ693 (VSJ693Q) /CSM/VS/VSJ6-D/VSJ693Q.Seq.d/                        959   0.0
VFE480 (VFE480Q) /CSM/VF/VFE4-D/VFE480Q.Seq.d/                        959   0.0
SSI374 (SSI374Q) /CSM/SS/SSI3-D/SSI374Q.Seq.d/                        959   0.0
SSF620 (SSF620Q) /CSM/SS/SSF6-A/SSF620Q.Seq.d/                        959   0.0
VHQ109 (VHQ109Q) /CSM/VH/VHQ1-A/VHQ109Q.Seq.d/                        954   0.0
SLH838 (SLH838Q) /CSM/SL/SLH8-B/SLH838Q.Seq.d/                        954   0.0
SSC346 (SSC346Q) /CSM/SS/SSC3-B/SSC346Q.Seq.d/                        952   0.0
VSI601 (VSI601Q) /CSM/VS/VSI6-A/VSI601Q.Seq.d/                        946   0.0
SFK520 (SFK520Q) /CSM/SF/SFK5-A/SFK520Q.Seq.d/                        946   0.0
VFH874 (VFH874Q) /CSM/VF/VFH8-D/VFH874Q.Seq.d/                        944   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF510846|AF510846.1 Dictyostelium discoideum calmodulin-binding protein ThyB (thyB) mRNA, complete cds.   959  0.0  1
U61990|U61990.1 Dictyostelium discoideum ThyB (thyB) mRNA, complete cds.   959  0.0  1
CB888459|CB888459.1 taa92g08.x1 Hydra EST -III Hydra magnipapillata cDNA 3' similar to SW:KITH_CHICK P04047 THYMIDINE KINASE, CYTOSOLIC ;, mRNA sequence.    56  2e-06  2
AZ692494|AZ692494.1 ENTJU18TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    48  0.002  2
CB823537|CB823537.1 EST 4761 Ripe Apricot Fruit Lambda Zap II Library Prunus armeniaca cDNA clone bh006p21 3', mRNA sequence.    50  0.026  1
CB823884|CB823884.1 EST 5108 Ripe Apricot Fruit Lambda Zap II Library Prunus armeniaca cDNA clone bh002g05 3', mRNA sequence.    50  0.026  1
BG661802|BG661802.1 kx06e10.y1 Parastrongyloides trichosuri IL SL1 TOPO v2 Parastrongyloides trichosuri cDNA similar to SW:KITH_CAPVK P16600 THYMIDINE KINASE ;, mRNA sequence.    44  0.22  2
AC027323|AC027323.5 Homo sapiens chromosome 5 clone CTD-2034A10, complete sequence.    46  0.35  3
AC010622|AC010622.4 Homo sapiens chromosome 5 clone CTD-2064E13, WORKING DRAFT SEQUENCE, 21 ordered pieces.    46  0.41  1
AC024288|AC024288.9 Homo sapiens, clone RP11-23B5, complete sequence.    46  0.41  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P04183) RecName: Full=Thymidine kinase, cytosolic;          EC=...   183   2e-45
AY335565_1(AY335565|pid:none) Synthetic construct Homo sapiens t...   183   2e-45
BT030694_1(BT030694|pid:none) Bos taurus thymidine kinase 1, sol...   182   3e-45
K02581_1(K02581|pid:none) Human thymidine kinase mRNA, complete ...   182   4e-45
AB451268_1(AB451268|pid:none) Homo sapiens TK1 mRNA for thymidin...   182   5e-45
DQ441416_85(DQ441416|pid:none) Variola virus strain Benin, Dahom...   181   1e-44
(P04364) RecName: Full=Thymidine kinase;          EC=2.7.1.21;  ...   181   1e-44
AM905441_1(AM905441|pid:none) Suberites domuncula mRNA for thymi...   180   1e-44
(P09768) RecName: Full=Thymidine kinase, cytosolic;          EC=...   180   1e-44
(P68563) RecName: Full=Thymidine kinase;          EC=2.7.1.21;  ...   180   2e-44

 psg: 0.68  gvh: 0.25  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall

>> prediction for VFE480 is nuc

>VFE480Z.Seq
----------AAGAATTAAAAGATTTAATTTTGCAAACAAAAAATGCTTATTAATTAAAT
ATAGTAAAGATACTAGATATAACGATAACATTGATAAATCATTTTTAGTTACCCATGACA
AACAAAATTATCAAGCTTTCCCATGTTCAATTTTAGAAGATGTTAAAGAACAAGCACAAA
ACTATGATGTAATTGGTATTGATGAAGGCCAATTTTTCCCAGACGTTGTACAATTTAGTG
AAGACTTGGCAAATCAAGGTAAANCCGTTATAATTGCCGCACTAGATGGTACCTTCCAAA
GAAAACCATTCCAATCAGTCATTGATTTAGTATCAAAGGCTGAATATATTACAAAGTTAA
CTGCCGTTTGTATGGTTTGCTACAATGAAGCCGCCTTTTCAAAACGTATCGTCGAAAGTG
ATGATATCGAATTAATTGGTGGTATAGATAAATATATTTCCGTTTGCCGTGGTTGCTACA
ATAGTGATCAAAATGAA