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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFE411
Library VF
(Link to library)
Clone ID VFE411
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14191-1
Original site URL
Representative seq. ID VFE411P
(Link to Original site)
Representative DNA sequence 
>VFE411 (VFE411Q) /CSM/VF/VFE4-A/VFE411Q.Seq.d/
AATTGTTTATTTATTCACCAAAATGCTTTCATTCAAATTCGCCAAATCTGCCTCAAAGGT
TATTGGAAATAGAAATTTCCACTCAAGTTCTGCTTCTTTAGCAAATACAACTAAATTATT
TATTAATGGTAAATTTGTTGAATCAAAAACTAAAGAATGGCTCGAAGTTAGAAATCCAGC
CACTCAAGAACTTGTAACTAAAGTACCAGTTTCAACTAAAGAAGAGATGGAGGCCGCAGT
TAAAGCAGCATCAGATGCTTTCCCAGCATGGAGAGATACATCAGTTTCAAACAGATCAAG
AATCATTAGCAACTATAAGAATTTAATCAATAAAAATATGGATAAAATTGCAGCAATCAT
TACAGAAGAACAAGGTAAAACCCTCCCAGACGCAGAGGGTGATGTTTTCAGAGGTTTAGA
AGTTGTTGAACATTCAGTCAATGTTGCATCATTAATGATGGGTGAAACCGTTGAAAATGT
TTCAAAGAATGTCGATATCTATTCATACGTTCAACCATTAGGTGTTTGTGCAGGTATCAC
TCCATTCAATTTCCCAGCAATGATTCCATTATGGATGTTCCCATTAGCAATTGCATGTGG
TAACACTTTTGTTTTGAAACCATCAGAACGTGTACCATCAGCATCXXXXXXXXXXAGAAC
GTACTTTAGATGCATTGACTGGTGCTGCTTTTGGTGCCTCTGGTCAACGTTGTATGGCTT
TAAGTGCTGCTGTATTCGTTGGTGAATCTAAAAATTGGATTCCAGAATTAGCTGAACGTG
CCAAGAAATTAAAGGTTGCAGGTGGTGCTGCACCAGGTGCTGATTTAGGTCCAGTGATTT
CAGCAGCCTCAAAACAAAGAATTCACGAACTCATTCAATCTGGTATTGATGAAGGTGCCA
AATGTATTTTAGATGGTCGTAACGTTGTCGTTGATCCAGAATTTTCAAAGGGTAACTTTG
TTGGTCCAACCATTCTCACTGATGTCAAACCACATATGAGATGTTACAAAGAGGAAATCT
TTGGTCCAGTTTTAGTTTGTCTCAATGTTGATACTGTCGATCAAGCCATTCAACTCATCA
ATGCTAACCCATACGGTAATGGTACTGCTGTCTTCACCTCATCTGGTGCTGTCGCTCGTA
AATATCAACGTGAAATCGATGTTGGTCAAGTTGGTATCAATTTACCAATCCCAGTTCCAC
TTCCATTCTTCTCCTTCACTGGTTCTCGTGGTTCTTTTGTTGGTGCTGGTCACTTCTACG
GTAAGACTGGTGTTCAATTCTTTACTCAAATTAAAACAATCACTTCAAACTGGAGAGATG
ATGATATTTCACACGGTGTTTCTTCATCAATGAATATGCCAATTTTAGGTAAAAATTAAA
AATTTAATTAATTAAAAAATATAATAAAAAAATAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
IVYLFTKMLSFKFAKSASKVIGNRNFHSSSASLANTTKLFINGKFVESKTKEWLEVRNPA
TQELVTKVPVSTKEEMEAAVKAASDAFPAWRDTSVSNRSRIISNYKNLINKNMDKIAAII
TEEQGKTLPDAEGDVFRGLEVVEHSVNVASLMMGETVENVSKNVDIYSYVQPLGVCAGIT
PFNFPAMIPLWMFPLAIACGNTFVLKPSERVPSA---

---ERTLDALTGAAFGASGQRCMALSAAVFVGESKNWIPELAERAKKLKVAGGAAPGADL
GPVISAASKQRIHELIQSGIDEGAKCILDGRNVVVDPEFSKGNFVGPTILTDVKPHMRCY
KEEIFGPVLVCLNVDTVDQAIQLINANPYGNGTAVFTSSGAVARKYQREIDVGQVGINLP
IPVPLPFFSFTGSRGSFVGAGHFYGKTGVQFFTQIKTITSNWRDDDISHGVSSSMNMPIL
GKN*kfn*lkniikk*
Translated Amino Acid sequence (All Frames) 
Frame A:
nclfihqnafiqirqiclkgywk*kfplkfcffskyn*iiy*w*ic*ikn*rmars*kss
hsrtcn*stsfn*rrdggrs*ssircfpsmeryisfkqiknh*ql*efnq*kyg*ncsnh
yrrtr*npprrrg*cfqrfrsc*tfsqcciindg*nr*kcfkecrylfirstirclcryh
siqfpsndsimdvpisncmw*hfcfetirtctisi---

---rtyfrcidwccfwclwstlygfkcccirw*i*kldsris*tcqeikgcrwcctrc*f
rssdfsslktknsrthsiwy**rcqmyfrws*rcr*srifkg*lcwsnhsh*cqttyeml
qrgnlwssfslsqc*ycrsshsthqc*pir*wycclhliwccrs*ist*nrcwsswyqft
npsstsilllhwfswffcwcwsllr*dwcsilysn*nnhfkler**yftrcffineyanf
r*klki*likkynkki

Frame B:
IVYLFTKMLSFKFAKSASKVIGNRNFHSSSASLANTTKLFINGKFVESKTKEWLEVRNPA
TQELVTKVPVSTKEEMEAAVKAASDAFPAWRDTSVSNRSRIISNYKNLINKNMDKIAAII
TEEQGKTLPDAEGDVFRGLEVVEHSVNVASLMMGETVENVSKNVDIYSYVQPLGVCAGIT
PFNFPAMIPLWMFPLAIACGNTFVLKPSERVPSA---

---ERTLDALTGAAFGASGQRCMALSAAVFVGESKNWIPELAERAKKLKVAGGAAPGADL
GPVISAASKQRIHELIQSGIDEGAKCILDGRNVVVDPEFSKGNFVGPTILTDVKPHMRCY
KEEIFGPVLVCLNVDTVDQAIQLINANPYGNGTAVFTSSGAVARKYQREIDVGQVGINLP
IPVPLPFFSFTGSRGSFVGAGHFYGKTGVQFFTQIKTITSNWRDDDISHGVSSSMNMPIL
GKN*kfn*lkniikk*

Frame C:
lfiyspkcfhsnspnlpqrlleieistqvlll*qiqlnyllmvnllnqklkngskleiqp
lknl*lkyqfqlkkrwrpqlkqhqmlsqhgeihqfqtdqeslatiri*sikiwiklqqsl
qknkvkpsqtqrvmfsev*kllniqsmlhh**wvkplkmfqrmsisihtfnh*vfvqvsl
hsisqq*fhygcsh*qlhvvtllf*nhqnvyhqh---

---nvl*mh*lvlllvplvnvvwl*vllyslvnlkigfqn*lnvprn*rlqvvlhqvli*
vq*fqqpqnkeftnsfnlvlmkvpnvf*mvvtlsliqnfqrvtllvqpfslmsnhi*dvt
krkslvqf*fvsmlilsikpfnssmlthtvmvllssphlvlslvninvksmlvklvsiyq
sqfhfhsspslvlvvlllvlvtstvrlvfnsllklkqslqtgemmifhtvflhq*icqf*
vkiknlin*ki**kn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFE411 (VFE411Q) /CSM/VF/VFE4-A/VFE411Q.Seq.d/                       2654   0.0
VFF882 (VFF882Q) /CSM/VF/VFF8-D/VFF882Q.Seq.d/                       1415   0.0
VFI639 (VFI639Q) /CSM/VF/VFI6-B/VFI639Q.Seq.d/                       1413   0.0
VFB842 (VFB842Q) /CSM/VF/VFB8-B/VFB842Q.Seq.d/                       1413   0.0
VHB389 (VHB389Q) /CSM/VH/VHB3-D/VHB389Q.Seq.d/                       1409   0.0
CFG290 (CFG290Q) /CSM/CF/CFG2-D/CFG290Q.Seq.d/                       1407   0.0
VFC565 (VFC565Q) /CSM/VF/VFC5-C/VFC565Q.Seq.d/                       1358   0.0
VFG168 (VFG168Q) /CSM/VF/VFG1-C/VFG168Q.Seq.d/                       1322   0.0
SFA764 (SFA764Q) /CSM/SF/SFA7-C/SFA764Q.Seq.d/                       1235   0.0
SFH845 (SFH845Q) /CSM/SF/SFH8-B/SFH845Q.Seq.d/                       1223   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

CD230130|CD230130.1 SS1_41_C04.b1_A012 Salt-stressed seedlings Sorghum bicolor cDNA clone SS1_41_C04_A012 3', mRNA sequence.    82  3e-21  3
BM393668|BM393668.1 50072-2-10-C10.f.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    52  2e-18  4
BM392567|BM392567.1 50071-2-10-C10.f.1 Chilcoat/Turkewitz cDNA (small fraction) Tetrahymena thermophila cDNA, mRNA sequence.    52  2e-18  4
AE015613|AE015613.1 Shewanella oneidensis MR-1 section 162 of 457 of the complete genome.    52  2e-14  5
AY255673|AY255673.1 Triticum aestivum methylmalonate semialdehyde dehydrogenase mRNA, partial cds; nuclear gene for mitochondrial product.    54  3e-11  3
BM396124|BM396124.1 5009-0-17-F02.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    48  8e-10  3
AK128924|AK128924.1 Mus musculus cDNA fis, clone TRACH3001119, highly similar to Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial precursor (EC 1.2.1.27).    44  9e-10  4
AF297860|AF297860.1 Mus musculus methylmalonate-semialdehyde dehydrogenase mRNA, complete cds.    44  9e-10  4
BC033440|BC033440.1 Mus musculus aldehyde dehydrogenase family 6, subfamily A1, mRNA (cDNA clone MGC:28587 IMAGE:4212084), complete cds.    44  9e-10  4
BC031148|BC031148.1 Mus musculus, Similar to RIKEN cDNA 1110038I05 gene, clone MGC:37325 IMAGE:4975560, mRNA, complete cds.    44  1e-09  4
dna update 2003. 9.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q7QC84) RecName: Full=Probable methylmalonate-semialdehyde dehy...   321   4e-86
(Q29HB2) RecName: Full=Probable methylmalonate-semialdehyde dehy...   303   1e-80
BT031194_1(BT031194|pid:none) Drosophila melanogaster GH07313 fu...   301   3e-80
BC108615_1(BC108615|pid:none) Xenopus laevis hypothetical protei...   291   5e-77
BT072671_1(BT072671|pid:none) Salmo salar clone ssal-rgf-535-350...   287   6e-76
BT045940_1(BT045940|pid:none) Salmo salar clone ssal-rgf-537-096...   287   6e-76
(Q02253) RecName: Full=Methylmalonate-semialdehyde dehydrogenase...   284   5e-75
(Q0WM29) RecName: Full=Methylmalonate-semialdehyde dehydrogenase...   283   8e-75
AK230004_1(AK230004|pid:none) Arabidopsis thaliana mRNA for F15N...   283   8e-75
M93405_1(M93405|pid:none) Human methylmalonate semialdehyde dehy...   283   1e-74
protein update 2009. 6.19
PSORT 

 psg: 0.74  gvh: 0.38  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.42  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane

>> prediction for VFE411 is mit
5' end seq. ID VFE411F
5' end seq. 
>VFE411F.Seq
AATTGTTTATTTATTCACCAAAATGCTTTCATTCAAATTCGCCAAATCTGCCTCAAAGGT
TATTGGAAATAGAAATTTCCACTCAAGTTCTGCTTCTTTAGCAAATACAACTAAATTATT
TATTAATGGTAAATTTGTTGAATCAAAAACTAAAGAATGGCTCGAAGTTAGAAATCCAGC
CACTCAAGAACTTGTAACTAAAGTACCAGTTTCAACTAAAGAAGAGATGGAGGCCGCAGT
TAAAGCAGCATCAGATGCTTTCCCAGCATGGAGAGATACATCAGTTTCAAACAGATCAAG
AATCATTAGCAACTATAAGAATTTAATCAATAAAAATATGGATAAAATTGCAGCAATCAT
TACAGAAGAACAAGGTAAAACCCTCCCAGACGCAGAGGGTGATGTTTTCAGAGGTTTAGA
AGTTGTTGAACATTCAGTCAATGTTGCATCATTAATGATGGGTGAAACCGTTGAAAATGT
TTCAAAGAATGTCGATATCTATTCATACGTTCAACCATTAGGTGTTTGTGCAGGTATCAC
TCCATTCAATTTCCCAGCAATGATTCCATTATGGATGTTCCCATTAGCAATTGCATGTGG
TAACACTTTTGTTTTGAAACCATCAGAACGTGTACCATCAGCATC----------
Length of 5' end seq. 645
3' end seq. ID VFE411Z
3' end seq. 
>VFE411Z.Seq
----------AGAACGTACTTTAGATGCATTGACTGGTGCTGCTTTTGGTGCCTCTGGTC
AACGTTGTATGGCTTTAAGTGCTGCTGTATTCGTTGGTGAATCTAAAAATTGGATTCCAG
AATTAGCTGAACGTGCCAAGAAATTAAAGGTTGCAGGTGGTGCTGCACCAGGTGCTGATT
TAGGTCCAGTGATTTCAGCAGCCTCAAAACAAAGAATTCACGAACTCATTCAATCTGGTA
TTGATGAAGGTGCCAAATGTATTTTAGATGGTCGTAACGTTGTCGTTGATCCAGAATTTT
CAAAGGGTAACTTTGTTGGTCCAACCATTCTCACTGATGTCAAACCACATATGAGATGTT
ACAAAGAGGAAATCTTTGGTCCAGTTTTAGTTTGTCTCAATGTTGATACTGTCGATCAAG
CCATTCAACTCATCAATGCTAACCCATACGGTAATGGTACTGCTGTCTTCACCTCATCTG
GTGCTGTCGCTCGTAAATATCAACGTGAAATCGATGTTGGTCAAGTTGGTATCAATTTAC
CAATCCCAGTTCCACTTCCATTCTTCTCCTTCACTGGTTCTCGTGGTTCTTTTGTTGGTG
CTGGTCACTTCTACGGTAAGACTGGTGTTCAATTCTTTACTCAAATTAAAACAATCACTT
CAAACTGGAGAGATGATGATATTTCACACGGTGTTTCTTCATCAATGAATATGCCAATTT
TAGGTAAAAATTAAAAATTTAATTAATTAAAAAATATAATAAAAAAATAA
Length of 3' end seq. 760
Connected seq. ID VFE411P
Connected seq. 
>VFE411P.Seq
AATTGTTTATTTATTCACCAAAATGCTTTCATTCAAATTCGCCAAATCTGCCTCAAAGGT
TATTGGAAATAGAAATTTCCACTCAAGTTCTGCTTCTTTAGCAAATACAACTAAATTATT
TATTAATGGTAAATTTGTTGAATCAAAAACTAAAGAATGGCTCGAAGTTAGAAATCCAGC
CACTCAAGAACTTGTAACTAAAGTACCAGTTTCAACTAAAGAAGAGATGGAGGCCGCAGT
TAAAGCAGCATCAGATGCTTTCCCAGCATGGAGAGATACATCAGTTTCAAACAGATCAAG
AATCATTAGCAACTATAAGAATTTAATCAATAAAAATATGGATAAAATTGCAGCAATCAT
TACAGAAGAACAAGGTAAAACCCTCCCAGACGCAGAGGGTGATGTTTTCAGAGGTTTAGA
AGTTGTTGAACATTCAGTCAATGTTGCATCATTAATGATGGGTGAAACCGTTGAAAATGT
TTCAAAGAATGTCGATATCTATTCATACGTTCAACCATTAGGTGTTTGTGCAGGTATCAC
TCCATTCAATTTCCCAGCAATGATTCCATTATGGATGTTCCCATTAGCAATTGCATGTGG
TAACACTTTTGTTTTGAAACCATCAGAACGTGTACCATCAGCATC----------AGAAC
GTACTTTAGATGCATTGACTGGTGCTGCTTTTGGTGCCTCTGGTCAACGTTGTATGGCTT
TAAGTGCTGCTGTATTCGTTGGTGAATCTAAAAATTGGATTCCAGAATTAGCTGAACGTG
CCAAGAAATTAAAGGTTGCAGGTGGTGCTGCACCAGGTGCTGATTTAGGTCCAGTGATTT
CAGCAGCCTCAAAACAAAGAATTCACGAACTCATTCAATCTGGTATTGATGAAGGTGCCA
AATGTATTTTAGATGGTCGTAACGTTGTCGTTGATCCAGAATTTTCAAAGGGTAACTTTG
TTGGTCCAACCATTCTCACTGATGTCAAACCACATATGAGATGTTACAAAGAGGAAATCT
TTGGTCCAGTTTTAGTTTGTCTCAATGTTGATACTGTCGATCAAGCCATTCAACTCATCA
ATGCTAACCCATACGGTAATGGTACTGCTGTCTTCACCTCATCTGGTGCTGTCGCTCGTA
AATATCAACGTGAAATCGATGTTGGTCAAGTTGGTATCAATTTACCAATCCCAGTTCCAC
TTCCATTCTTCTCCTTCACTGGTTCTCGTGGTTCTTTTGTTGGTGCTGGTCACTTCTACG
GTAAGACTGGTGTTCAATTCTTTACTCAAATTAAAACAATCACTTCAAACTGGAGAGATG
ATGATATTTCACACGGTGTTTCTTCATCAATGAATATGCCAATTTTAGGTAAAAATTAAA
AATTTAATTAATTAAAAAATATAATAAAAAAATAA
Length of connected seq. 1405
Full length Seq ID -
Full length Seq. -
Length of full length seq. -