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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM386
Library AF
(Link to library)
Clone ID AFM386
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM386P
(Link to Original site)
Representative DNA sequence 
>AFM386 (AFM386Q) /CSM/AF/AFM3-D/AFM386Q.Seq.d/
AGAACTGTTACAGCAGTAAATGGTCCACTCGTTATTTTAGATAATATCAGAGGTCCAAAA
TTCTCTGAAATTGTAACTTTAACTTTAGGTGATGGTACTCAAAGAAAAGGTCAAGTTTTG
GAAATCCAAGGTAAAAGAGCTGTTGTACAAGTTTTCGAAGGTACAATTGGTATTGATGCC
AAACACACTCGTTGTGAATTCTCTGGTGATATTTTAAAGATGCCAGTATCAGAAGATTCA
TTAGGTCGTATTTTCAATGGTTCAGGTAAACCAGTTGATAAAGGTCCAAATGTTTTAGCT
GAAGAATACTTAGATATTCAAGGTCAACCAATCAATCCATCAGTTAGAGTTTACCCACAA
GAAATGATTCAAACTGGTATCTCTGCTATTGATACTATGAATTCAATTGCTCGTGGTCAA
AAGATTCCAATTTTCTCTGCTGCAGGTTTACCACACAATGAAATTGCCGCTCAAATTTGT
CGTCAAGCTGGTTTAGTTAAAAAATCAGGTAAAGGTGTTATTGATGATCATGAAGATAAT
TTTGCTATTGTATTCGCAGCTATGGGTGTTAATATGGAAACTGCATCATTTTTCAAACGT
GATTTCGAXXXXXXXXXXCTTGCATTGACCACCGCCGAATATCTTGCATATCAATGTGAA
AAACACGTTTTTAGTTCTTTTAACCGATATGTCTTCATATGCTGATGCTCTTCGTGAGGT
ATCTGCTGCTCGTGAAGAAGTACCAGGTCGTCGTGGTTATCCAGGTTATATGTATACAGA
TCTTTCAACCATTTACGAACGTGCTGGTCGTATTCAAGGTCGTAATGGTTCTATCACTCA
AATTCCAATTTTGACTATGCCAAATGATGATATTACCCATCCAATTCCAGATTTAACTGG
TTACATTACTGAAGGTCAAATTTTCATTGATCGTCAAATCAATAACAGACAAATCTACCC
ACCAATCAATGTATTACCATCACTTTCTCGTCTTATGAAATCCGCCATTGGTGACGACAT
GACCCGTGGCGATCACTCTGAAGTCTCAAATCAAATGTATGCCAACTATGCCATCGGTAA
AGATGTCCAAGCTATGAAAGCTGTCGTTGGTGAGGAAGCACTTTCTTCTGAAGATAAACT
CTACTTAGAATTTTTAGAACGTTTCGAATCTAGCTTTGTCGGTCAAAACCATTATGAAAA
TCGTGATATCTTTAATTCATTGGATCTTGGTTGGTCTTTATTAAGAACTTTCCCAAGCAA
TCTCCTCAAACGTATCACTGAAAAAACTATTAAACAATACTATTCACGTTCATCAAAGGG
TACTGTAGATTCAACTAACTAAATTTTAAAAAGAAAAAAATATAA
sequence update 2009. 4. 3
Translated Amino Acid sequence 
RTVTAVNGPLVILDNIRGPKFSEIVTLTLGDGTQRKGQVLEIQGKRAVVQVFEGTIGIDA
KHTRCEFSGDILKMPVSEDSLGRIFNGSGKPVDKGPNVLAEEYLDIQGQPINPSVRVYPQ
EMIQTGISAIDTMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSGKGVIDDHEDN
FAIVFAAMGVNMETASFFKRDF---

---LALTTAEYLAYQCEKHVFSSFNRYVFIC*css*giccs*rstrsswlsrlyvyrsfn
hlrtcwsysrs*wfyhsnsnfdyak**yypsnsrfnwlhy*rsnfh*ssnq*qtnlptnq
cititfssyeirhw*rhdpwrsl*slksnvcqlchr*rcpsyescrw*gstff*r*tllr
ifrtfri*lcrskpl*ks*yl*figswlvfiknfpkqspqtyh*kny*tilftfikgycr
fn*lnfkkkki*
Translated Amino Acid sequence (All Frames) 
Frame A:
RTVTAVNGPLVILDNIRGPKFSEIVTLTLGDGTQRKGQVLEIQGKRAVVQVFEGTIGIDA
KHTRCEFSGDILKMPVSEDSLGRIFNGSGKPVDKGPNVLAEEYLDIQGQPINPSVRVYPQ
EMIQTGISAIDTMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSGKGVIDDHEDN
FAIVFAAMGVNMETASFFKRDF---

---LALTTAEYLAYQCEKHVFSSFNRYVFIC*css*giccs*rstrsswlsrlyvyrsfn
hlrtcwsysrs*wfyhsnsnfdyak**yypsnsrfnwlhy*rsnfh*ssnq*qtnlptnq
cititfssyeirhw*rhdpwrsl*slksnvcqlchr*rcpsyescrw*gstff*r*tllr
ifrtfri*lcrskpl*ks*yl*figswlvfiknfpkqspqtyh*kny*tilftfikgycr
fn*lnfkkkki*

Frame B:
ellqq*mvhslf*iisevqnslkl*l*l*vmvlkekvkfwkskvkellykfskvqlvlmp
ntlvvnslvif*rcqyqkih*vvfsmvqvnqlikvqmf*lknt*ifkvnqsihqlefthk
k*fklvslllil*iqllvvkrfqfsllqvyhtmklplkfvvklv*lknqvkvllmimkii
lllysqlwvliwklhhfsnvis---

---lh*pppnilhinvkntflvlltdmssyadalrevsaareevpgrrgypgymytdlst
iyeragriqgrngsitqipiltmpnddithpipdltgyitegqifidrqinnrqiyppin
vlpslsrlmksaigddmtrgdhsevsnqmyanyaigkdvqamkavvgeealssedklyle
flerfessfvgqnhyenrdifnsldlgwsllrtfpsnllkritektikqyysrsskgtvd
stn*ilkrkky

Frame C:
ncysskwstryfr*yqrskil*ncnfnfr*wyskkrssfgnpr*kscctsfrrynwy*cq
thsl*ilw*yfkdasirrfirsyfqwfr*ts**rskcfs*rilrysrstnqsis*slptr
ndsnwylcy*yyefncswskdsnflccrfttq*ncrsnlssswfs*kir*rcy**s*r*f
cycirsygc*ygnciifqt*fr---

---cidhrriscism*ktrf*ff*piclhmlmlfvrylllvkkyqvvvviqviciqifqp
ftnvlvvfkvvmvlslkfqf*lcqmmilpiqfqi*lvtllkvkfslivksitdksthqsm
yyhhflvl*npplvtt*pvaitlksqikcmptmpsvkmskl*klslvrkhfllkinst*n
f*nvsnlalsvktimkivislihwilvgly*elsqaissnvslkkllnntihvhqrvl*i
qltkf*kekni
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM386 (AFM386Q) /CSM/AF/AFM3-D/AFM386Q.Seq.d/                       2611   0.0
CHB350 (CHB350Q) /CSM/CH/CHB3-C/CHB350Q.Seq.d/                       1429   0.0
AFM777 (AFM777Q) /CSM/AF/AFM7-D/AFM777Q.Seq.d/                       1429   0.0
AFC794 (AFC794Q) /CSM/AF/AFC7-D/AFC794Q.Seq.d/                       1429   0.0
CHB733 (CHB733Q) /CSM/CH/CHB7-B/CHB733Q.Seq.d/                       1427   0.0
SFK761 (SFK761Q) /CSM/SF/SFK7-C/SFK761Q.Seq.d/                       1423   0.0
CFJ409 (CFJ409Q) /CSM/CF/CFJ4-A/CFJ409Q.Seq.d/                       1423   0.0
AFK619 (AFK619Q) /CSM/AF/AFK6-A/AFK619Q.Seq.d/                       1423   0.0
AFH841 (AFH841Q) /CSM/AF/AFH8-B/AFH841Q.Seq.d/                       1413   0.0
AFO705 (AFO705Q) /CSM/AF/AFO7-A/AFO705Q.Seq.d/                       1407   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ347033) Dictyostelium discoideum cDNA clone:dda25l21, 3' ...  1445   0.0    1
(AC117081) Dictyostelium discoideum chromosome 2 map 5862124...  1346   0.0    2
(BJ401247) Dictyostelium discoideum cDNA clone:dds22j15, 3' ...  1346   0.0    2
(BJ377653) Dictyostelium discoideum cDNA clone:ddc25b09, 3' ...  1346   0.0    2
(BJ377426) Dictyostelium discoideum cDNA clone:ddc24d13, 3' ...  1346   0.0    2
(BJ375513) Dictyostelium discoideum cDNA clone:ddc19b04, 3' ...  1346   0.0    2
(BJ375428) Dictyostelium discoideum cDNA clone:ddc18m17, 3' ...  1346   0.0    2
(BJ375262) Dictyostelium discoideum cDNA clone:ddc18f02, 3' ...  1346   0.0    2
(BJ347357) Dictyostelium discoideum cDNA clone:dda26j19, 3' ...  1346   0.0    2
(BJ341495) Dictyostelium discoideum cDNA clone:dda6l23, 3' e...  1346   0.0    2
dna update 2009. 4.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q76NU1) RecName: Full=V-type proton ATPase subunit B;          ...   445   0.0
EU727173_1(EU727173|pid:none) Bombyx mori strain NB vacuolar ATP...   353   0.0
(P31410) RecName: Full=V-type proton ATPase subunit B;          ...   352   0.0
AY197611_1(AY197611|pid:none) Artemia franciscana vacuolar proto...   353   0.0
AF037468_1(AF037468|pid:none) Culex pipiens quinquefasciatus B s...   352   0.0
BC115214_1(BC115214|pid:none) Danio rerio ATPase, H+ transportin...   357   0.0
AF472615_1(AF472615|pid:none) Danio rerio vacuolar-type H+ trans...   354   0.0
AY891921_1(AY891921|pid:none) Synthetic construct Homo sapiens c...   355   0.0
B44138(B44138;A39212;S29665) H+-exporting ATPase (EC 3.6.3.6) 56...   355   0.0
BC007309_1(BC007309|pid:none) Homo sapiens ATPase, H+ transporti...   355   0.0
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM386 is

5' end seq. ID AFM386F
5' end seq. 
>AFM386F.Seq
AGAACTGTTACAGCAGTAAATGGTCCACTCGTTATTTTAGATAATATCAGAGGTCCAAAA
TTCTCTGAAATTGTAACTTTAACTTTAGGTGATGGTACTCAAAGAAAAGGTCAAGTTTTG
GAAATCCAAGGTAAAAGAGCTGTTGTACAAGTTTTCGAAGGTACAATTGGTATTGATGCC
AAACACACTCGTTGTGAATTCTCTGGTGATATTTTAAAGATGCCAGTATCAGAAGATTCA
TTAGGTCGTATTTTCAATGGTTCAGGTAAACCAGTTGATAAAGGTCCAAATGTTTTAGCT
GAAGAATACTTAGATATTCAAGGTCAACCAATCAATCCATCAGTTAGAGTTTACCCACAA
GAAATGATTCAAACTGGTATCTCTGCTATTGATACTATGAATTCAATTGCTCGTGGTCAA
AAGATTCCAATTTTCTCTGCTGCAGGTTTACCACACAATGAAATTGCCGCTCAAATTTGT
CGTCAAGCTGGTTTAGTTAAAAAATCAGGTAAAGGTGTTATTGATGATCATGAAGATAAT
TTTGCTATTGTATTCGCAGCTATGGGTGTTAATATGGAAACTGCATCATTTTTCAAACGT
GATTTCGAXXXXXXXXXX
Length of 5' end seq. 618
3' end seq. ID AFM386Z
3' end seq. 
>AFM386Z.Seq
XXXXXXXXXXCTTGCATTGACCACCGCCGAATATCTTGCATATCAATGTGAAAAACACGT
TTTTAGTTCTTTTAACCGATATGTCTTCATATGCTGATGCTCTTCGTGAGGTATCTGCTG
CTCGTGAAGAAGTACCAGGTCGTCGTGGTTATCCAGGTTATATGTATACAGATCTTTCAA
CCATTTACGAACGTGCTGGTCGTATTCAAGGTCGTAATGGTTCTATCACTCAAATTCCAA
TTTTGACTATGCCAAATGATGATATTACCCATCCAATTCCAGATTTAACTGGTTACATTA
CTGAAGGTCAAATTTTCATTGATCGTCAAATCAATAACAGACAAATCTACCCACCAATCA
ATGTATTACCATCACTTTCTCGTCTTATGAAATCCGCCATTGGTGACGACATGACCCGTG
GCGATCACTCTGAAGTCTCAAATCAAATGTATGCCAACTATGCCATCGGTAAAGATGTCC
AAGCTATGAAAGCTGTCGTTGGTGAGGAAGCACTTTCTTCTGAAGATAAACTCTACTTAG
AATTTTTAGAACGTTTCGAATCTAGCTTTGTCGGTCAAAACCATTATGAAAATCGTGATA
TCTTTAATTCATTGGATCTTGGTTGGTCTTTATTAAGAACTTTCCCAAGCAATCTCCTCA
AACGTATCACTGAAAAAACTATTAAACAATACTATTCACGTTCATCAAAGGGTACTGTAG
ATTCAACTAACTAAATTTTAAAAAGAAAAAAATATAA
Length of 3' end seq. 757
Connected seq. ID AFM386P
Connected seq. 
>AFM386P.Seq
AGAACTGTTACAGCAGTAAATGGTCCACTCGTTATTTTAGATAATATCAGAGGTCCAAAA
TTCTCTGAAATTGTAACTTTAACTTTAGGTGATGGTACTCAAAGAAAAGGTCAAGTTTTG
GAAATCCAAGGTAAAAGAGCTGTTGTACAAGTTTTCGAAGGTACAATTGGTATTGATGCC
AAACACACTCGTTGTGAATTCTCTGGTGATATTTTAAAGATGCCAGTATCAGAAGATTCA
TTAGGTCGTATTTTCAATGGTTCAGGTAAACCAGTTGATAAAGGTCCAAATGTTTTAGCT
GAAGAATACTTAGATATTCAAGGTCAACCAATCAATCCATCAGTTAGAGTTTACCCACAA
GAAATGATTCAAACTGGTATCTCTGCTATTGATACTATGAATTCAATTGCTCGTGGTCAA
AAGATTCCAATTTTCTCTGCTGCAGGTTTACCACACAATGAAATTGCCGCTCAAATTTGT
CGTCAAGCTGGTTTAGTTAAAAAATCAGGTAAAGGTGTTATTGATGATCATGAAGATAAT
TTTGCTATTGTATTCGCAGCTATGGGTGTTAATATGGAAACTGCATCATTTTTCAAACGT
GATTTCGA----------CTTGCATTGACCACCGCCGAATATCTTGCATATCAATGTGAA
AAACACGTTTTTAGTTCTTTTAACCGATATGTCTTCATATGCTGATGCTCTTCGTGAGGT
ATCTGCTGCTCGTGAAGAAGTACCAGGTCGTCGTGGTTATCCAGGTTATATGTATACAGA
TCTTTCAACCATTTACGAACGTGCTGGTCGTATTCAAGGTCGTAATGGTTCTATCACTCA
AATTCCAATTTTGACTATGCCAAATGATGATATTACCCATCCAATTCCAGATTTAACTGG
TTACATTACTGAAGGTCAAATTTTCATTGATCGTCAAATCAATAACAGACAAATCTACCC
ACCAATCAATGTATTACCATCACTTTCTCGTCTTATGAAATCCGCCATTGGTGACGACAT
GACCCGTGGCGATCACTCTGAAGTCTCAAATCAAATGTATGCCAACTATGCCATCGGTAA
AGATGTCCAAGCTATGAAAGCTGTCGTTGGTGAGGAAGCACTTTCTTCTGAAGATAAACT
CTACTTAGAATTTTTAGAACGTTTCGAATCTAGCTTTGTCGGTCAAAACCATTATGAAAA
TCGTGATATCTTTAATTCATTGGATCTTGGTTGGTCTTTATTAAGAACTTTCCCAAGCAA
TCTCCTCAAACGTATCACTGAAAAAACTATTAAACAATACTATTCACGTTCATCAAAGGG
TACTGTAGATTCAACTAACTAAATTTTAAAAAGAAAAAAATATAA
Length of connected seq. 1355
Full length Seq ID -
Full length Seq. -
Length of full length seq. -