>VFE256 (VFE256Q) /CSM/VF/VFE2-C/VFE256Q.Seq.d/
AATCAAATATTATTATAATAAAAGATTGGTAAAAAACCATGTCAAAATTATTAATTTTAT
TGTTATTATCATTGGTTGCTTCAATCTTTTCAACTCCATTGGATGATTATGTCAATGCTC
CTGACGATACCTACAAGTGGACATTAAATAATACAATTGAATATGAAACTTTTACAGGTT
ACATTTTAGAGTTGACATCACAAACATGGATGGCTGAAAAATCAGATTGGCCAGTTTGGA
AACATTGGGTTTCAATTTGTGTACCAAAAGGTGTTACAACTACAACTACATTTATATATG
TTGATGGTGGTTCAAATGATAACTGGAAAGTACCAGGTTCAATGGATCAAACAATTGAAA
TTGTTTGTCTTTCATCTGGTTCAGTTAGTGTTGGTTTAACTCAAATTCCAAATCAACCAA
TCATTTTCAATAATGATGGTGTTCAAAGATTTGAAGATGATCTCGTTGCTTATACTTGGA
GACAATTTTTAGGAAATACAAGTGAACXXXXXXXXXXGTTTTAACAGNACCANAAGGTGG
NATCATTCCANACAAAGTTAAAGTTTGGACAGCAGTAACTGAAAGNACAACCANAANANA
TTTCCGNATCATCACATGNATGGATATCNCCAAATGNATTCAATTCATCATTTGGGATCC
TTNTGANATTACNCCAACTTCCNCTGGNGNANATTCAATCNCTTTATCAAAACCANATGC
TGGTTGGANAGCNTTNTTCCTTGAAGCTGAANATTTANATGCTAAAAATTCNATCGATGA
TGAANANACNCTTAAATTCNCNTCTGAAGNTGCCNTTGGACCAAANACNTTACCNTTCGG

sniiiikdw*KTMSKLLILLLLSLVASIFSTPLDDYVNAPDDTYKWTLNNTIEYETFTGY
ILELTSQTWMAEKSDWPVWKHWVSICVPKGVTTTTTFIYVDGGSNDNWKVPGSMDQTIEI
VCLSSGSVSVGLTQIPNQPIIFNNDGVQRFEDDLVAYTWRQFLGNTSE---

---VLTXPXGGIIPXKVKVWTAVTEXTTXXXFRIITXMDIXKXIQFIIWDPXXITPTSXG
XXSIXLSKPXAGWXAXFLEAEXLXAKNSIDDEXTLKFXSEXAXGPXTLPF

Frame A:
nqill**kigkkpcqny*fycyyhwllqsfqlhwmimsmlltiptsgh*iiqlnmkllqv
tf*s*hhkhgwlknqigqfgnigfqfvyqkvlqlqlhlymlmvvqmitgkyqvqwikqlk
lfvfhlvqlvlv*lkfqinqsfsimmvfkdlkmisllilgdnf*eiqvn---

---VLTXPXGGIIPXKVKVWTAVTEXTTXXXFRIITXMDIXKXIQFIIWDPXXITPTSXG
XXSIXLSKPXAGWXAXFLEAEXLXAKNSIDDEXTLKFXSEXAXGPXTLPF

Frame B:
ikyyynkrlvknhvkiinfiviiigcfnlfnsig*lcqcs*rylqvdik*yn*i*nfyrl
hfrvditnmdg*kirlasletlgfnlctkrcynynyiyic*wwfk**lestrfngsnn*n
clsfiwfs*cwfnsnskstnhfq**wcski*r*srclyletifrkyk*---

---f*qxxkvxsfxtklkfgqq*lkxqpxxisxsshxwispnxfnssfgilxxlxqlplx
xiqslyqnxmlvgxxxslklxixmlkixsmmxxxlnsxlkxpldqxxyxs

Frame C:
sniiiikdw*KTMSKLLILLLLSLVASIFSTPLDDYVNAPDDTYKWTLNNTIEYETFTGY
ILELTSQTWMAEKSDWPVWKHWVSICVPKGVTTTTTFIYVDGGSNDNWKVPGSMDQTIEI
VCLSSGSVSVGLTQIPNQPIIFNNDGVQRFEDDLVAYTWRQFLGNTSE---

---fnxtxrwxhsxqs*sldssn*kxnxxxfpxhhmxgyxqmxsihhlgsx*xyxnfxwx
xfnxfiktxcwlxsxxp*s*xfxc*kfxr**xxx*ixx*xcxwtkxxtxr

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFE256 (VFE256Q) /CSM/VF/VFE2-C/VFE256Q.Seq.d/                       1386   0.0
VFN176 (VFN176Q) /CSM/VF/VFN1-D/VFN176Q.Seq.d/                       1005   0.0
VFL666 (VFL666Q) /CSM/VF/VFL6-C/VFL666Q.Seq.d/                       1005   0.0
VFL194 (VFL194Q) /CSM/VF/VFL1-D/VFL194Q.Seq.d/                       1005   0.0
VFI702 (VFI702Q) /CSM/VF/VFI7-A/VFI702Q.Seq.d/                       1005   0.0
VFI335 (VFI335Q) /CSM/VF/VFI3-B/VFI335Q.Seq.d/                       1005   0.0
VFI263 (VFI263Q) /CSM/VF/VFI2-C/VFI263Q.Seq.d/                       1005   0.0
VFF783 (VFF783Q) /CSM/VF/VFF7-D/VFF783Q.Seq.d/                       1005   0.0
VFD645 (VFD645Q) /CSM/VF/VFD6-B/VFD645Q.Seq.d/                       1005   0.0
VFD303 (VFD303Q) /CSM/VF/VFD3-A/VFD303Q.Seq.d/                       1005   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116989|AC116989.2 Dictyostelium discoideum chromosome 2 map complement(3527391-3470188) strain AX4, complete sequence.    38  0.025  5
BX088589|BX088589.1 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone  DKEY-27E7.    42  0.23  3
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.    32  0.29  6
Z68213|Z68213.1 Caenorhabditis elegans cosmid C01F6.              44  0.51  2
AU086601|AU086601.1 Plasmodium falciparum 3D7 cDNA clone from Sugano malaria cDNA library: XPFn3021.    42  0.59  2
BD140494|BD140494.1 Elongation factor-2 kinase (EF-2 kinase) and methods of use therefor.    38  0.64  2
AR193041|AR193041.1 Sequence 7 from patent US 6346406.            38  0.64  2
U90946|U90946.1 Dictyostelium discoideum myosin heavy chain kinase B (MHCK-B) mRNA, complete cds.    38  0.64  2
AZ202314|AZ202314.1 SP_0056_A2_F09_SP6E Strongylocentrotus purpuratus, purple sea urchin, sperm genomic BAC library Strongylocentrotus purpuratus genomic clone Plate=56 Col=18 Row=K, DNA sequence.    46  0.70  1
BX147732|BX147732.1 Danio rerio genomic clone DKEY-106C4, genomic survey sequence.    46  0.70  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q5XM24) RecName: Full=Autocrine proliferation repressor; AltNam...   288   1e-76
AC116987_4(AC116987|pid:none) Dictyostelium discoideum chromosom...   120   7e-26
CP000473_2389(CP000473|pid:none) Solibacter usitatus Ellin6076, ...   101   3e-20
CP000127_1294(CP000127|pid:none) Nitrosococcus oceani ATCC 19707...    58   3e-07
AE015451_4974(AE015451|pid:none) Pseudomonas putida KT2440 compl...    53   1e-05
CP000712_4860(CP000712|pid:none) Pseudomonas putida F1, complete...    52   2e-05
AE003849_1380(AE003849|pid:none) Xylella fastidiosa 9a5c, comple...    51   5e-05
AE008922_2895(AE008922|pid:none) Xanthomonas campestris pv. camp...    49   2e-04
AM920689_1230(AM920689|pid:none) Xanthomonas campestris pv. camp...    49   2e-04
CU694393_139(CU694393|pid:none) Ralstonia solanacearum strain Mo...    49   3e-04

 psg: 0.97  gvh: 0.90  alm: 0.39  top: 0.13  tms: 0.00  mit: 0.42  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: extracellular, including cell wall
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: cytoplasmic
	  4.0 %: mitochondrial

>> prediction for VFE256 is exc

>VFE256F.Seq
AATCAAATATTATTATAATAAAAGATTGGTAAAAAACCATGTCAAAATTATTAATTTTAT
TGTTATTATCATTGGTTGCTTCAATCTTTTCAACTCCATTGGATGATTATGTCAATGCTC
CTGACGATACCTACAAGTGGACATTAAATAATACAATTGAATATGAAACTTTTACAGGTT
ACATTTTAGAGTTGACATCACAAACATGGATGGCTGAAAAATCAGATTGGCCAGTTTGGA
AACATTGGGTTTCAATTTGTGTACCAAAAGGTGTTACAACTACAACTACATTTATATATG
TTGATGGTGGTTCAAATGATAACTGGAAAGTACCAGGTTCAATGGATCAAACAATTGAAA
TTGTTTGTCTTTCATCTGGTTCAGTTAGTGTTGGTTTAACTCAAATTCCAAATCAACCAA
TCATTTTCAATAATGATGGTGTTCAAAGATTTGAAGATGATCTCGTTGCTTATACTTGGA
GACAATTTTTAGGAAATACAAGTGAAC----------

>VFE256Z.Seq
----------GTTTTAACAGNACCANAAGGTGGNATCATTCCANACAAAGTTAAAGTTTG
GACAGCAGTAACTGAAAGNACAACCANAANANATTTCCGNATCATCACATGNATGGATAT
CNCCAAATGNATTCAATTCATCATTTGGGATCCTTNTGANATTACNCCAACTTCCNCTGG
NGNANATTCAATCNCTTTATCAAAACCANATGCTGGTTGGANAGCNTTNTTCCTTGAAGC
TGAANATTTANATGCTAAAAATTCNATCGATGATGAANANACNCTTAAATTCNCNTCTGA
AGNTGCCNTTGGACCAAANACNTTACCNTTCGG

>VFE256P.Seq
AATCAAATATTATTATAATAAAAGATTGGTAAAAAACCATGTCAAAATTATTAATTTTAT
TGTTATTATCATTGGTTGCTTCAATCTTTTCAACTCCATTGGATGATTATGTCAATGCTC
CTGACGATACCTACAAGTGGACATTAAATAATACAATTGAATATGAAACTTTTACAGGTT
ACATTTTAGAGTTGACATCACAAACATGGATGGCTGAAAAATCAGATTGGCCAGTTTGGA
AACATTGGGTTTCAATTTGTGTACCAAAAGGTGTTACAACTACAACTACATTTATATATG
TTGATGGTGGTTCAAATGATAACTGGAAAGTACCAGGTTCAATGGATCAAACAATTGAAA
TTGTTTGTCTTTCATCTGGTTCAGTTAGTGTTGGTTTAACTCAAATTCCAAATCAACCAA
TCATTTTCAATAATGATGGTGTTCAAAGATTTGAAGATGATCTCGTTGCTTATACTTGGA
GACAATTTTTAGGAAATACAAGTGAAC----------GTTTTAACAGNACCANAAGGTGG
NATCATTCCANACAAAGTTAAAGTTTGGACAGCAGTAACTGAAAGNACAACCANAANANA
TTTCCGNATCATCACATGNATGGATATCNCCAAATGNATTCAATTCATCATTTGGGATCC
TTNTGANATTACNCCAACTTCCNCTGGNGNANATTCAATCNCTTTATCAAAACCANATGC
TGGTTGGANAGCNTTNTTCCTTGAAGCTGAANATTTANATGCTAAAAATTCNATCGATGA
TGAANANACNCTTAAATTCNCNTCTGAAGNTGCCNTTGGACCAAANACNTTACCNTTCGG