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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFE249
Library VF
(Link to library)
Clone ID VFE249
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16317-1
Original site URL
Representative seq. ID VFE249P
(Link to Original site)
Representative DNA sequence 
>VFE249 (VFE249Q) /CSM/VF/VFE2-C/VFE249Q.Seq.d/
ATTTTTTTAAACACACTAACACATAAATAATATTTATCTATCTATCCACACACCCAAAGT
ATTCATATTAATGGCAGAAATAACAATATCAATTGAAAATGAAAATTTCATTCAAGTAAA
TTTACAACCAGATCAAACAGTTGAAGATCTAAAACGTAGAGTAGAATTTGAAACCACAAT
TTTAGTGAACAATCAAGTTTTAACACTTGATGGAAAAGTATTAGATAATGAAAAGAAATT
ATCAGACTATTCAATTAAAGGTGGTGATTTCTTATTGATAACAAAAAATGTTTTAAGAGC
ACCACAACAAAGATCACAACAACCCCAACAACCACAACAACCACAACAACCACAACAACA
ACGTCAACCACAAAGAGATCCTCTCAATTCACCACAAGATATTTTAGACCACTTTACAAA
CAATCCTGAAGACTTAXXXXXXXXXXACTTCAACCACTACCACTACCACTACCACTACTT
CACCCCAAACTCCAACTAATGCCACTTCTCCTGTAAATAATAATAATAATAATAATAACA
ACAACAACAACACTAGACCTCCAATTAATTCACCACCAGTTGCAAAGACACCGCAACCAA
CACCACAACCAACTACAAATCAATATGCTTCTGAATCCAATATCCAAACCCTTATTGGAC
TTGGTTGTTCAAGAGATAAGGCAATTAGACTTTTAACTCAAGCTAAAGGCAATGTTGATG
TTGCTGCTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ff*th*HINNIYLSIHTPKVFILMAEITISIENENFIQVNLQPDQTVEDLKRRVEFETTI
LVNNQVLTLDGKVLDNEKKLSDYSIKGGDFLLITKNVLRAPQQRSQQPQQPQQPQQPQQQ
RQPQRDPLNSPQDILDHFTNNPEDL---

---TSTTTTTTTTTSPQTPTNATSPVNNNNNNNNNNNNTRPPINSPPVAKTPQPTPQPTT
NQYASESNIQTLIGLGCSRDKAIRLLTQAKGNVDVAA
Translated Amino Acid sequence (All Frames) 
Frame A:
iflntlthk*ylsiyphtqsihingrnnnin*k*kfhsskfttrsns*rskt*sri*nhn
fseqssfnt*wksir**keiirlfn*rw*flidnkkcfkstttkitttpttttttttttt
tsttkrssqfttryfrplykqs*rl---

---TSTTTTTTTTTSPQTPTNATSPVNNNNNNNNNNNNTRPPINSPPVAKTPQPTPQPTT
NQYASESNIQTLIGLGCSRDKAIRLLTQAKGNVDVAA

Frame B:
ff*th*HINNIYLSIHTPKVFILMAEITISIENENFIQVNLQPDQTVEDLKRRVEFETTI
LVNNQVLTLDGKVLDNEKKLSDYSIKGGDFLLITKNVLRAPQQRSQQPQQPQQPQQPQQQ
RQPQRDPLNSPQDILDHFTNNPEDL---

---lqplplplpllhpklqlmplll*iiiiiiitttttldlqlihhqlqrhrnqhhnqlq
inmllnpiskplldlvvqeirqldf*lklkamlmlll

Frame C:
ffkhtnt*iifiylsthpkysy*wqk*qyqlkmkisfk*iynqikqlki*nve*nlkpqf
**tikf*hlmeky*imkrnyqtiqlkvvisy**qkmf*ehhnkdhnnpnnhnnhnnhnnn
vnhkeilsihhkif*ttlqtilkt---

---fnhyhyhyhyftpnsn*chfsck*******qqqqh*tsn*fttsckdtatntttnyk
sicf*iqypnpywtwlfkr*gn*tfnss*rqc*ccc
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFE249 (VFE249Q) /CSM/VF/VFE2-C/VFE249Q.Seq.d/                        577   e-164
VFE196 (VFE196Q) /CSM/VF/VFE1-D/VFE196Q.Seq.d/                        577   e-164
SFL472 (SFL472Q) /CSM/SF/SFL4-C/SFL472Q.Seq.d/                        577   e-164
SFL211 (SFL211Q) /CSM/SF/SFL2-A/SFL211Q.Seq.d/                        577   e-164
SFK703 (SFK703Q) /CSM/SF/SFK7-A/SFK703Q.Seq.d/                        577   e-164
SFJ734 (SFJ734Q) /CSM/SF/SFJ7-B/SFJ734Q.Seq.d/                        577   e-164
SFJ357 (SFJ357Q) /CSM/SF/SFJ3-C/SFJ357Q.Seq.d/                        577   e-164
SFG344 (SFG344Q) /CSM/SF/SFG3-B/SFG344Q.Seq.d/                        577   e-164
SFF829 (SFF829Q) /CSM/SF/SFF8-B/SFF829Q.Seq.d/                        577   e-164
SFF802 (SFF802Q) /CSM/SF/SFF8-A/SFF802Q.Seq.d/                        577   e-164
own update 2001.11.28
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AQ935615|AQ935615.1 CpG2561B CpIOWAgDNA1 Cryptosporidium parvum genomic, DNA sequence.    54  3e-05  2
AC115598|AC115598.2 Dictyostelium discoideum chromosome 2 map 581427-735498 strain AX4, complete sequence.    48  3e-04  5
AC123513|AC123513.1 Dictyostelium discoideum chromosome 2 map 2779865-2840915 strain AX4, *** SEQUENCING IN PROGRESS ***, in ordered pieces.    58  3e-04  7
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    56  5e-04  5
AC117070|AC117070.2 Dictyostelium discoideum chromosome 2 map 2097701-2233941 strain AX4, complete sequence.    58  5e-04  3
AY162212|AY162212.1 Dictyostelium discoideum kinesin 1 gene, partial cds.    56  6e-04  1
AC119130|AC119130.3 Rattus norvegicus clone CH230-414I23, *** SEQUENCING IN PROGRESS ***, 20 unordered pieces.    54  7e-04  5
AC125395|AC125395.1 Mus musculus chromosome UNK clone RP23-213J1, WORKING DRAFT SEQUENCE, 11 unordered pieces.    52  9e-04  3
AC103123|AC103123.5 Rattus norvegicus clone CH230-226B23, WORKING DRAFT SEQUENCE, 1 ordered piece.    52  0.001  3
AC098951|AC098951.5 Rattus norvegicus clone CH230-173E15, WORKING DRAFT SEQUENCE, 5 unordered pieces.    56  0.001  3
dna update 2003. 9.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54JB0) RecName: Full=Protein DDI1 homolog;                          184   3e-45
AK067974_1(AK067974|pid:none) Oryza sativa Japonica Group cDNA c...    52   1e-05
BT058466_1(BT058466|pid:none) Salmo salar clone Contig1073 UV ex...    46   0.001
BC055645_1(BC055645|pid:none) Danio rerio neural precursor cell ...    43   0.009
BT073010_1(BT073010|pid:none) Oncorhynchus mykiss clone omyk-evn...    43   0.009
BC078600_1(BC078600|pid:none) Xenopus laevis hypothetical protei...    43   0.009
BT075193_1(BT075193|pid:none) Osmerus mordax clone omor-eva-507-...    43   0.009
BC076245_1(BC076245|pid:none) Danio rerio neural precursor cell ...    43   0.009
BT083291_1(BT083291|pid:none) Anoplopoma fimbria clone afim-evh-...    43   0.009
EF103394_1(EF103394|pid:none) Haliotis discus discus ubiquitin-l...    42   0.015
protein update 2009. 6.18
PSORT 

 psg: 0.83  gvh: 0.33  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: plasma membrane

>> prediction for VFE249 is cyt
5' end seq. ID VFE249F
5' end seq. 
>VFE249F.Seq
ATTTTTTTAAACACACTAACACATAAATAATATTTATCTATCTATCCACACACCCAAAGT
ATTCATATTAATGGCAGAAATAACAATATCAATTGAAAATGAAAATTTCATTCAAGTAAA
TTTACAACCAGATCAAACAGTTGAAGATCTAAAACGTAGAGTAGAATTTGAAACCACAAT
TTTAGTGAACAATCAAGTTTTAACACTTGATGGAAAAGTATTAGATAATGAAAAGAAATT
ATCAGACTATTCAATTAAAGGTGGTGATTTCTTATTGATAACAAAAAATGTTTTAAGAGC
ACCACAACAAAGATCACAACAACCCCAACAACCACAACAACCACAACAACCACAACAACA
ACGTCAACCACAAAGAGATCCTCTCAATTCACCACAAGATATTTTAGACCACTTTACAAA
CAATCCTGAAGACTTA----------
Length of 5' end seq. 436
3' end seq. ID VFE249Z
3' end seq. 
>VFE249Z.Seq
----------ACTTCAACCACTACCACTACCACTACCACTACTTCACCCCAAACTCCAAC
TAATGCCACTTCTCCTGTAAATAATAATAATAATAATAATAACAACAACAACAACACTAG
ACCTCCAATTAATTCACCACCAGTTGCAAAGACACCGCAACCAACACCACAACCAACTAC
AAATCAATATGCTTCTGAATCCAATATCCAAACCCTTATTGGACTTGGTTGTTCAAGAGA
TAAGGCAATTAGACTTTTAACTCAAGCTAAAGGCAATGTTGATGTTGCTGCTT
Length of 3' end seq. 283
Connected seq. ID VFE249P
Connected seq. 
>VFE249P.Seq
ATTTTTTTAAACACACTAACACATAAATAATATTTATCTATCTATCCACACACCCAAAGT
ATTCATATTAATGGCAGAAATAACAATATCAATTGAAAATGAAAATTTCATTCAAGTAAA
TTTACAACCAGATCAAACAGTTGAAGATCTAAAACGTAGAGTAGAATTTGAAACCACAAT
TTTAGTGAACAATCAAGTTTTAACACTTGATGGAAAAGTATTAGATAATGAAAAGAAATT
ATCAGACTATTCAATTAAAGGTGGTGATTTCTTATTGATAACAAAAAATGTTTTAAGAGC
ACCACAACAAAGATCACAACAACCCCAACAACCACAACAACCACAACAACCACAACAACA
ACGTCAACCACAAAGAGATCCTCTCAATTCACCACAAGATATTTTAGACCACTTTACAAA
CAATCCTGAAGACTTA----------ACTTCAACCACTACCACTACCACTACCACTACTT
CACCCCAAACTCCAACTAATGCCACTTCTCCTGTAAATAATAATAATAATAATAATAACA
ACAACAACAACACTAGACCTCCAATTAATTCACCACCAGTTGCAAAGACACCGCAACCAA
CACCACAACCAACTACAAATCAATATGCTTCTGAATCCAATATCCAAACCCTTATTGGAC
TTGGTTGTTCAAGAGATAAGGCAATTAGACTTTTAACTCAAGCTAAAGGCAATGTTGATG
TTGCTGCTT
Length of connected seq. 719
Full length Seq ID -
Full length Seq. -
Length of full length seq. -