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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM380
Library AF
(Link to library)
Clone ID AFM380
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM380P
(Link to Original site)
Representative DNA sequence 
>AFM380 (AFM380Q) /CSM/AF/AFM3-D/AFM380Q.Seq.d/
AAAATTATTTCCCACCCAAAAGCACACCATAACATTGTATTTATATATAAATATTATCAC
ACAAATTATTAAATTATTACTAGTATATATAATTATTTAGAATAATAAAATAATAATAAT
AAAAATAATAATAACCGAATTTTAAAATTAATAAAAACTAAACAACTATAATATATAAAT
AGATTTAAAAAAAAAATATATAAAAAATGTCATCAGATATTTCAGAAAAAATTCAACAAG
CAAGAAGAGATGCTGAATCAATGAAAGAACAAATTAGAGCAAACAGAGATGTAATGAATG
ATACTACTCTCAAAACTTTTACTCGTGATTTACCAGGTCTTCCAAAGATGGAAGGTAAAA
TTAAAGTTAGAAGAAACCTTAAGGGTCATCTTGCTAAAATCTATGCTATGCATTGGGCTG
AAGATAATGTTCATTTAGTTTCTGCCTCTCAAGATGGTAAATTATTAGTTTGGGATGGTT
TAACTACCAACAAAGTCCACGCTATTCCATTAAGATCATCATGGGTTATGACATGTGCTT
ATTCACCAACTGCTAACCTTGTTXXXXXXXXXXCCAAATTATGGGATTTACGTAGTGGTA
AATGTGTTCAAACTTTCACTGGTCACGAAGCTGATATTAATGCAGTTCAATATTTCCCAA
ATGGTTTATCATTTGGNACTGGTTNTGATNATGCTTCATGTAGATTATTTGATATTCGTG
CCGATCGTGAATTAATGCAATAC
sequence update 2009. 4. 3
Translated Amino Acid sequence 
nyfppkstp*hciyi*ilshkllnyy*yi*lfriik****k***pnfkinkn*ttiiyk*
i*kkni*KMSSDISEKIQQARRDAESMKEQIRANRDVMNDTTLKTFTRDLPGLPKMEGKI
KVRRNLKGHLAKIYAMHWAEDNVHLVSASQDGKLLVWDGLTTNKVHAIPLRSSWVMTCAY
SPTANLV---

---KLWDLRSGKCVQTFTGHEADINAVQYFPNGLSFGTGXDXASCRLFDIRADRELMQY
Translated Amino Acid sequence (All Frames) 
Frame A:
kiishpkahhnivfiykyyhtny*iitsiynyle**nnnnknnnnrilkliktkql*yin
rfkkkiykkchqifqkkfnkqeemlnq*knkleqtem**millsklllviyqvfqrwkvk
lkleetlrvillksmlciglkimfi*flplkmvny*fgmv*lptkstlfh*dhhgl*hvl
ihqlltl---

---pnygiyvvvnvfklslvtklilmqfnisqmvyhlxlvxxmlhvdylifvpivn*cn

Frame B:
klfptqkhtitlylyiniitqiiklllvyiii*nnkiiiikiiitef*n**klnnyni*i
dlkkkyiknviryfrknstskkrc*inertn*skqrcne*yysqnfys*ftrsskdgr*n
*s*kkp*gssc*nlcyalg*r*csfsfclsrw*iislgwfnyqqsprysikiimgydmcl
ftnc*pc---

---qimgft*w*mcsnfhwsrs*y*cssifpkwfiiwxwx*xcfm*ii*yscrs*inai

Frame C:
nyfppkstp*hciyi*ilshkllnyy*yi*lfriik****k***pnfkinkn*ttiiyk*
i*kkni*KMSSDISEKIQQARRDAESMKEQIRANRDVMNDTTLKTFTRDLPGLPKMEGKI
KVRRNLKGHLAKIYAMHWAEDNVHLVSASQDGKLLVWDGLTTNKVHAIPLRSSWVMTCAY
SPTANLV---

---KLWDLRSGKCVQTFTGHEADINAVQYFPNGLSFGTGXDXASCRLFDIRADRELMQY
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM380 (AFM380Q) /CSM/AF/AFM3-D/AFM380Q.Seq.d/                       1007   0.0
SLA519 (SLA519Q) /CSM/SL/SLA5-A/SLA519Q.Seq.d/                        720   0.0
SFB734 (SFB734Q) /CSM/SF/SFB7-B/SFB734Q.Seq.d/                        720   0.0
AFO746 (AFO746Q) /CSM/AF/AFO7-B/AFO746Q.Seq.d/                        720   0.0
AFO321 (AFO321Q) /CSM/AF/AFO3-A/AFO321Q.Seq.d/                        720   0.0
VFK342 (VFK342Q) /CSM/VF/VFK3-B/VFK342Q.Seq.d/                        718   0.0
AFJ370 (AFJ370Q) /CSM/AF/AFJ3-C/AFJ370Q.Seq.d/                        712   0.0
AFK519 (AFK519Q) /CSM/AF/AFK5-A/AFK519Q.Seq.d/                        708   0.0
SSD702 (SSD702Q) /CSM/SS/SSD7-A/SSD702Q.Seq.d/                        301   8e-81
SLD136 (SLD136Q) /CSM/SL/SLD1-B/SLD136Q.Seq.d/                        272   7e-72
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(X73641) Dictyostelium discoideum gene for G-protein beta-su...   682   0.0    2
(AC116977) Dictyostelium discoideum chromosome 2 map 5515173...   406   0.0    6
(BJ329696) Dictyostelium discoideum cDNA clone:dda25p19, 5' ...   706   0.0    2
(BJ327867) Dictyostelium discoideum cDNA clone:dda22e05, 5' ...   686   0.0    2
(BJ415054) Dictyostelium discoideum cDNA clone:ddv21d11, 5' ...   696   0.0    2
(AU060176) Dictyostelium discoideum slug cDNA, clone SLA519.      698   0.0    1
(BJ387696) Dictyostelium discoideum cDNA clone:dds4d09, 5' e...   698   0.0    1
(BJ329002) Dictyostelium discoideum cDNA clone:dda30l11, 5' ...   698   0.0    1
(BJ328699) Dictyostelium discoideum cDNA clone:dda29j05, 5' ...   698   0.0    1
(BJ327222) Dictyostelium discoideum cDNA clone:dda19l17, 5' ...   690   0.0    1
dna update 2009. 4.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36408) RecName: Full=Guanine nucleotide-binding protein subuni...   242   1e-62
AF536325_1(AF536325|pid:none) Ustilago maydis G-protein beta sub...   158   1e-37
AY599846_1(AY599846|pid:none) Rhizopus stolonifer G-protein beta...   157   4e-37
AY884129_1(AY884129|pid:none) Thanatephorus cucumeris guanine nu...   155   1e-36
AF091120_1(AF091120|pid:none) Filobasidiella neoformans G-protei...   155   2e-36
AY211190_1(AY211190|pid:none) Cochliobolus heterostrophus G-prot...   153   4e-36
AY661671_1(AY661671|pid:none) Lentinula edodes guanine nucleotid...   152   1e-35
AB188197_1(AB188197|pid:none) Botryotinia fuckeliana bcgb1 gene ...   149   6e-35
AB086901_1(AB086901|pid:none) Magnaporthe grisea Mgb1 gene for h...   149   1e-34
AB072452_1(AB072452|pid:none) Fusarium oxysporum fgb1 gene for g...   147   2e-34
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM380 is

5' end seq. ID AFM380F
5' end seq. 
>AFM380F.Seq
AAAATTATTTCCCACCCAAAAGCACACCATAACATTGTATTTATATATAAATATTATCAC
ACAAATTATTAAATTATTACTAGTATATATAATTATTTAGAATAATAAAATAATAATAAT
AAAAATAATAATAACCGAATTTTAAAATTAATAAAAACTAAACAACTATAATATATAAAT
AGATTTAAAAAAAAAATATATAAAAAATGTCATCAGATATTTCAGAAAAAATTCAACAAG
CAAGAAGAGATGCTGAATCAATGAAAGAACAAATTAGAGCAAACAGAGATGTAATGAATG
ATACTACTCTCAAAACTTTTACTCGTGATTTACCAGGTCTTCCAAAGATGGAAGGTAAAA
TTAAAGTTAGAAGAAACCTTAAGGGTCATCTTGCTAAAATCTATGCTATGCATTGGGCTG
AAGATAATGTTCATTTAGTTTCTGCCTCTCAAGATGGTAAATTATTAGTTTGGGATGGTT
TAACTACCAACAAAGTCCACGCTATTCCATTAAGATCATCATGGGTTATGACATGTGCTT
ATTCACCAACTGCTAACCTTGTTXXXXXXXXXX
Length of 5' end seq. 573
3' end seq. ID AFM380Z
3' end seq. 
>AFM380Z.Seq
XXXXXXXXXXCCAAATTATGGGATTTACGTAGTGGTAAATGTGTTCAAACTTTCACTGGT
CACGAAGCTGATATTAATGCAGTTCAATATTTCCCAAATGGTTTATCATTTGGNACTGGT
TNTGATNATGCTTCATGTAGATTATTTGATATTCGTGCCGATCGTGAATTAATGCAATAC
Length of 3' end seq. 180
Connected seq. ID AFM380P
Connected seq. 
>AFM380P.Seq
AAAATTATTTCCCACCCAAAAGCACACCATAACATTGTATTTATATATAAATATTATCAC
ACAAATTATTAAATTATTACTAGTATATATAATTATTTAGAATAATAAAATAATAATAAT
AAAAATAATAATAACCGAATTTTAAAATTAATAAAAACTAAACAACTATAATATATAAAT
AGATTTAAAAAAAAAATATATAAAAAATGTCATCAGATATTTCAGAAAAAATTCAACAAG
CAAGAAGAGATGCTGAATCAATGAAAGAACAAATTAGAGCAAACAGAGATGTAATGAATG
ATACTACTCTCAAAACTTTTACTCGTGATTTACCAGGTCTTCCAAAGATGGAAGGTAAAA
TTAAAGTTAGAAGAAACCTTAAGGGTCATCTTGCTAAAATCTATGCTATGCATTGGGCTG
AAGATAATGTTCATTTAGTTTCTGCCTCTCAAGATGGTAAATTATTAGTTTGGGATGGTT
TAACTACCAACAAAGTCCACGCTATTCCATTAAGATCATCATGGGTTATGACATGTGCTT
ATTCACCAACTGCTAACCTTGTT----------CCAAATTATGGGATTTACGTAGTGGTA
AATGTGTTCAAACTTTCACTGGTCACGAAGCTGATATTAATGCAGTTCAATATTTCCCAA
ATGGTTTATCATTTGGNACTGGTTNTGATNATGCTTCATGTAGATTATTTGATATTCGTG
CCGATCGTGAATTAATGCAATAC
Length of connected seq. 733
Full length Seq ID -
Full length Seq. -
Length of full length seq. -